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Basic studies toward prevention of blood fluke infection of cultured bluefin tunainfection

Research Project

Project/Area Number 17H03865
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Aquatic bioproduction science
Research InstitutionMeguro Parasitological Museum

Principal Investigator

Ogawa Kazuo  公益財団法人目黒寄生虫館, その他部局等, 館長 (20092174)

Co-Investigator(Kenkyū-buntansha) 脇 司  東邦大学, 理学部, 講師 (50775963)
白樫 正  近畿大学, 水産研究所, 准教授 (70565936)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2019: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Keywords住血吸虫 / クロマグロ / 生活環 / 中間宿主 / 寄生虫 / 養殖 / 住血吸虫症 / フタエラフサゴカイ / スポロシスト / 対策
Outline of Final Research Achievements

Year-round monthly surveys of the intermediate host polychaetes proliferating on the net cages of tuna cages revealed that polychaetes became infected in summer, while in winter they grew in numbers but no infection occurred. Unexpectedly, removal of polychaetes on the net cages resulted in drastic decrease in the infection of the intermediate host. This suggests that eradication of polychaetes around the culture systems will be an effective control measure of the blood fluke infection of net cage cultured bluefin tuna.
We succeeded in in vitro maintenance of infected polychaetes by injecting sporocysts from naturally infected polychaetes into uninfected ones. This has enabled to maintain developmental stages of the blood fluke and to observe its development and proliferation in the intermediate host.

Academic Significance and Societal Importance of the Research Achievements

クロマグロ養殖場で発生するカルジコラ属住血吸虫による寄生虫病は産業的被害が大きい。駆虫剤が水産用医薬品として承認されたが、根本的な対策となっていない。そこで、予防法の開発を目指した。中間宿主ゴカイにおける感染時期が明らかになり、養殖場から中間宿主を駆除することがクロマグロにおける寄生を予防することにきわめて有効であるという結果も得られた。一方、自然感染ゴカイから得た住血吸虫のスポロシスト期幼生を無感染ゴカイに接種する人為感染にも成功した。感染ゴカイを維持することによって、これまで知見の乏しかった中間宿主内の住血吸虫の発育や増殖が実験室内で観察することが可能になった。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (8 results)

All 2020 2019 2018 2017

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results) Presentation (4 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] In vivo cultivation of tuna blood fluke Cardicola orientalis in terebellid intermediate hosts2020

    • Author(s)
      S. Shirakashi, T. Matsuda, N. Asai, T. Honryo and K. Ogawa
    • Journal Title

      International Journal for Parasitology

      Volume: in press Issue: 10-11 Pages: 851-857

    • DOI

      10.1016/j.ijpara.2020.04.006

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spatial and temporal changes in the distribution of blood fluke infection in Nicolea gracilibranchis (Polychaeta: Terebellidae)2017

    • Author(s)
      Shirakashi, S., K. Tani, K. Ishimaru, T. Honryo, S. P. Shin, H. Uchida and K. Ogawa
    • Journal Title

      Journal of Parasitology

      Volume: 103 (5) Issue: 5 Pages: 541-546

    • DOI

      10.1645/17-70

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Identification of Blood Flukes Infecting Tiger Puffer <i>Takifugu rubripes</i>2017

    • Author(s)
      Ogawa, K. and Shengfa Liu
    • Journal Title

      Fish Pathology

      Volume: 52 Issue: 3 Pages: 131-140

    • DOI

      10.3147/jsfp.52.131

    • NAID

      130006179600

    • ISSN
      0388-788X, 1881-7335
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Blood Fluke Infection of Japanese Amberjack <i>Seriola quinqueradiata</i> in Fish Farms along the Western Coastal Area of Bungo Channel, Japan2017

    • Author(s)
      Fukuda, Y., K. Miyamura, E. Hitaka, K. Kimoto, Y. Sanada, T. Asai and K. Ogawa
    • Journal Title

      Fish Pathology

      Volume: 52 Issue: 4 Pages: 191-197

    • DOI

      10.3147/jsfp.52.191

    • NAID

      130006321465

    • ISSN
      0388-788X, 1881-7335
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] Transplantation of fish blood fluke sporocysts to natural- and unnatural polychaete intermediate hosts2019

    • Author(s)
      S. Shirakashi, T. Matsuda, N. Asai and K. Ogawa
    • Organizer
      Annual Meeting of Canadian Society of Zoologists
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ヒラマサに寄生する住血吸虫2019

    • Author(s)
      小川 和夫, 杉原 志貴, 秋山 孝介, 福田 穣, D. Grabner
    • Organizer
      第88回日本寄生虫学会大会, 長崎県長崎市(長崎大学)
    • Related Report
      2018 Annual Research Report
  • [Presentation] Cardicola orientalis, the blood fluke of Pacific bluefin tuna, in the polychaete intermediate host.2018

    • Author(s)
      Ogawa, K., S. Shirakashi, T. Honryo and H. Uchida
    • Organizer
      14th International Congress of Parasitology, Daegu, Republic of Korea
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Possibility for non-drug control of blood fluke infections in farmed bluefin tuna.2018

    • Author(s)
      Shirakashi, S., Honryo, T., Kawahara, M.,Ogawa, K.
    • Organizer
      AQUA 2018, Le Corum, Montpellier.
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2021-02-19  

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