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Development of biosensor system for fish physiological state by using bidirectional communication technology

Research Project

Project/Area Number 17H03871
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Aquatic life science
Research InstitutionTokyo University of Marine Science and Technology

Principal Investigator

Endo Hideaki  東京海洋大学, 学術研究院, 教授 (50242326)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Keywordsバイオセンサ / 魚類 / モニタリング / 通信 / 双方向 / ストレス / 雌雄判別 / 産卵 / 生理 / バイオセンシング
Outline of Final Research Achievements

The aim of this research was to develop a novel bidirectional wireless biosensing system for easily and intuitively monitoring the physiological state of fish. The system allows for direct visual judgment of different states and real-time monitoring of fish stress according to the commands sent by the client. We first developed a biosensor and a bidirectional communication system for monitoring the physiologic state of fish, such as the stress response, and attached them to multiple test fish. Next, we tried to identify specific individuals swimming in the water by controlling the LED pattern remotely. The fish stress states could also be visualized automatically by changing the color of the LED (Red ⇔ Yellow ⇔ Green) through 2 different preset thresholds. The proposed biosensor system fulfilled the objectives of the research.

Academic Significance and Societal Importance of the Research Achievements

バイオセンサと双方向通信技術を用いることにより,魚類の生体内情報の可視化と定量的なモニタリングが可能なバイオセンシングシステムを構築することができた.これにより,魚を自由に遊泳させた状態で,その生理状態と各種センサによる応答値との関わりを容易かつ詳細に解析することが可能となった.本システムによって得られる新知見は,魚類行動学,魚類生理学,水産化学等の分野の横断的発展のために大いに貢献できる可能性があり,その学術的意義は極めて高いと考えられる.また,水族館や河川で発生した魚の不明死や疾病などに対する原因究明の手がかりとなるような知見も得られる可能性があり,本研究の社会的意義も大きいと考えている.

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (45 results)

All 2021 2020 2019 2018 2017

All Journal Article (14 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 12 results,  Open Access: 5 results,  Acknowledgement Compliant: 3 results) Presentation (29 results) (of which Int'l Joint Research: 14 results) Book (2 results)

  • [Journal Article] Development of biosensor for measuring oxidative stress of fish2021

    • Author(s)
      Wu Haiyun、Ogata Madoka、Ohnuki Hitoshi、Endo Hideaki
    • Journal Title

      Fisheries Science

      Volume: 87 Issue: 1 Pages: 151-159

    • DOI

      10.1007/s12562-020-01484-4

    • NAID

      40022461851

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Integrated Biosensor System for Monitoring and Visualizing Fish Stress Response2020

    • Author(s)
      Wu Haiyun、Murata Masataka、Matsumoto Haruto、Ohnuki Hitoshi、Endo Hideaki
    • Journal Title

      Sensors and Materials

      Volume: 32 Issue: 12 Pages: 4095

    • DOI

      10.18494/SAM.2020.3121

    • ISSN
      0914-4935, 2435-0869
    • Year and Date
      2020-12-10
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Monitoring of Fish Stress Response Using Biosensor System2020

    • Author(s)
      遠藤英明
    • Journal Title

      The Journal of The Institute of Electrical Engineers of Japan

      Volume: 140 Issue: 10 Pages: 676-677

    • DOI

      10.1541/ieejjournal.140.676

    • NAID

      130007920092

    • ISSN
      1340-5551, 1881-4190
    • Year and Date
      2020-10-01
    • Related Report
      2020 Annual Research Report
  • [Journal Article] Development of an enzyme-functionalized immunosensor for measuring maturation-inducing hormone in fish2020

    • Author(s)
      Wu Haiyun、Saito Yusuke、Yoshiura Yasutoshi、Ohnuki Hitoshi、Endo Hideaki
    • Journal Title

      Biochemical Engineering Journal

      Volume: 154 Pages: 107460-107460

    • DOI

      10.1016/j.bej.2019.107460

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a novel LED color-switching type biosensor system for the visualization of fish stress response2019

    • Author(s)
      H. Wu, R. Shinoda, M. Murata, H. Matsumoto, H. Ohnuki, H. Endo
    • Journal Title

      Anal. Methods

      Volume: 11 Issue: 43 Pages: 5623-5628

    • DOI

      10.1039/c9ay01752f

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biosensors for the assessment of fish health: a review2019

    • Author(s)
      H. Endo and H. Wu
    • Journal Title

      Fish. Sci.

      Volume: 85 Issue: 4 Pages: 641-654

    • DOI

      10.1007/s12562-019-01318-y

    • NAID

      40021934517

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Real-time fish stress visualization came true: a novel multi-stage color-switching wireless biosensor system2019

    • Author(s)
      H. Wu, R. Shinoda, M. Murata, H. Matsumoto, H. Ohnuki, H. Endo
    • Journal Title

      Biosens. Bioelecton.

      Volume: 130 Pages: 360-366

    • DOI

      10.1016/j.bios.2018.09.042

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Self-powered hydrogen peroxide sensor and its application as a biosensor2019

    • Author(s)
      H. Ohnuki, T. Wako, B. Mecheri, H. Wu, D. Tsuya, H. Endo
    • Journal Title

      Jap. J. Applied Physics

      Volume: 58 Issue: SB Pages: SBBG16-SBBG16

    • DOI

      10.7567/1347-4065/ab01d2

    • NAID

      210000135445

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Studies on development of biosensor system for fish health2018

    • Author(s)
      遠藤 英明
    • Journal Title

      NIPPON SUISAN GAKKAISHI

      Volume: 84 Issue: 3 Pages: 350-353

    • DOI

      10.2331/suisan.WA2524

    • NAID

      130007382225

    • ISSN
      0021-5392, 1349-998X
    • Related Report
      2018 Annual Research Report
  • [Journal Article] New approach for monitoring fish stress: A novel enzyme-functionalized label-free immunosensor system for detecting cortisol levels in fish2017

    • Author(s)
      H. Wu, H. Ohnuki, S. Ota, M. Murata, Y. Yoshiura, H. Endo
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 93 Pages: 57-64

    • DOI

      10.1016/j.bios.2016.10.001

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Flow immunosensor system with an electrode replacement unit for continuous cortisol monitoring for fish2017

    • Author(s)
      H. Wu, H. Ohnuki, M. Murata, H. End
    • Journal Title

      Sensing and Bio-Sensing Research

      Volume: 13 Pages: 122-127

    • DOI

      10.1016/j.sbsr.2017.01.002

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Development of an optical communication type biosensor for real-time monitoring of fish stress2017

    • Author(s)
      R. Shinoda, H. Wu, M. Murata, H. Ohnuki, Y. Yoshiura, H. Endo
    • Journal Title

      Sensors & Actuators: B. Chemical

      Volume: 247 Pages: 765-773

    • DOI

      10.1016/j.snb.2017.03.034

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Oriented antibody immobilization on self-assembled monolayers applied as impedance biosensors2017

    • Author(s)
      Kaiki Tsugimura, Hitoshi Ohnuki, Haiyun Wu, Hideaki Endo, Daiju Tsuya, and Mitsuru Izumi
    • Journal Title

      Journal of Physics: Conference Series

      Volume: 924 Pages: 012015-012015

    • DOI

      10.1088/1742-6596/924/1/012015

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ultra highly sensitive method for detecting Edwardsiella ictaluri using high gradient immunomagnetic separation with polymerase chain reaction2017

    • Author(s)
      H. Wu, K. Sugata, M. Hirai, Y. Yoshiura, K. Hibi, H. Endo
    • Journal Title

      Bio-Sens. Res.

      Volume: 16 Pages: 68-73

    • DOI

      10.1016/j.sbsr.2017.11.003

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Development of Self-Powered Glucose Biosensors for Fish Stress Monitoring2020

    • Author(s)
      H. Wu, L. Shen, H. Ohnuki, H. Endo
    • Organizer
      PRiME 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 魚類のためのグルコース測定用フレキシブルバイオセンサの試作に関する研究2020

    • Author(s)
      宇部ゆきの・呉 海雲・村田 政隆・松本 陽斗・大貫 等・遠藤英明
    • Organizer
      令和3年度日本水産学会秋季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 生体試料を用いた魚類のための酸化ストレス測定用バイオセンサの特性評価2020

    • Author(s)
      水野 伶,呉 海雲,大貫 等,遠藤 英明
    • Organizer
      令和2年度日本水産学会春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 魚のための自家発電型グルコースバイオセンサの改良に関する研究2020

    • Author(s)
      沈 立航,呉 海雲,大貫 等,遠藤 英明
    • Organizer
      令和2年度日本水産学会春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Visual Monitoring by A Wireless Biosensor System with Bi-Directional Communication Technology2019

    • Author(s)
      H. Wu, K. Yamada, M. Murata, H. Matsumoto, H. Ohnuki, H. Endo,
    • Organizer
      WSCE 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 魚類の酸化ストレス応答モニタリングのためのスーパーオキシド測定用バイオセンサの基礎的研究2019

    • Author(s)
      呉 海雲,尾形まどか,大貫 等,遠藤英明
    • Organizer
      令和元年度日本水産学会秋季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 自家発電型グルコースバイオセンサの試作に関する基礎的研究2019

    • Author(s)
      沈 立航,呉 海雲,大貫 等,遠藤英明
    • Organizer
      令和元年度日本水産学会秋季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 魚類のストレス応答測定のための双方向通信型バイオセンサシステムの試作2019

    • Author(s)
      呉 海雲,山田和輝,村田政隆,松本陽斗,大貫 等,遠藤英明
    • Organizer
      平成31年度日本水産学会春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] カーボンナノチューブを用いた魚類の雌雄判別のための11-Ketotestosterone測定用イムノセンサの試作2019

    • Author(s)
      斉藤祐介,宇部ゆきの,呉 海雲,吉崎悟朗,大貫 等,遠藤英明
    • Organizer
      平成31年度日本水産学会春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 魚類の腹水に着目したバイオセンサによる魚のストレス応答モニタリング2019

    • Author(s)
      平井愛美,呉 海雲,村田政隆,松本陽斗,大貫 等,遠藤英明
    • Organizer
      平成31年度日本水産学会春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Development of an enzyme-functionalized immunosensor system for detecting oocyte maturation-inducing hormone in fish2018

    • Author(s)
      Y. Saito, H. Wu, H. Ohnuki, H. Endo
    • Organizer
      Biosensors 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] LED color-switching type wireless biosensor system for the visualization of fish stress2018

    • Author(s)
      H. Wu, R. Shinoda, M. Murata, H. Matsumoto, H. Ohnuki, H. Endo
    • Organizer
      Biosensors 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Micro-gapped parallel plate electrodes: a new design for electrochemical impedance biosensor2018

    • Author(s)
      H. Ohnuki, Y. Kusaka, H. Wu, H. Endo, D. Tsuya
    • Organizer
      Biosensors 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Biosensor system for simultaneous stress visualizing and monitoring for fish using optical communication technology2018

    • Author(s)
      N. Hirai, H. Wu, M. Murata, H. Matsumoto, H. Ohnuki, H. Endo
    • Organizer
      3rd Aquaculture Conference 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Novel remote bio-sensing system by using bi-directional communication technology for fish stress real-time monitoring2018

    • Author(s)
      K. Yamada, H. Wu, M. Murata, H. Matsumoto, H. Ohnuki, H. Endo
    • Organizer
      3rd Aquaculture Conference 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fish stress monitoring system by continuous measuring cortisol using microfluidic bio-sensing technology2018

    • Author(s)
      H. Wu, K. Yoshida, H. Ohnuki, Y. Yoshiura, H. Endo
    • Organizer
      3rd Aquaculture Conference 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Self-Powered Hydrogen Peroxise Sensor and Its Biosensor Application2018

    • Author(s)
      H. Ohnuki, T. Wako, B. Mecheri, H. Wu, D. Tsuya, H. Endo
    • Organizer
      International Conference on Solid State Devices and Materials
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 櫛型電極ミオグロビンバイオセンサの再生技術の探索2018

    • Author(s)
      藤城志遥,大貫等,津谷大樹, 呉海雲,遠藤英明
    • Organizer
      第66回 応用物理学会春季学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 電気化学インピーダンスバイオセンサにおける非特異吸着の除去2018

    • Author(s)
      本田翔陽,大貫 等,津谷大樹,呉 海雲,遠藤英明
    • Organizer
      第66回 応用物理学会春季学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 電気化学インピーダンス法によるマルチセンシング平行平板型電極の開発2018

    • Author(s)
      本田翔陽,大貫 等,津谷大樹,呉 海雲,遠藤英明
    • Organizer
      第79回応用物理学会秋季学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 魚類のための双方向電波通信型バイオセンサシステムの試作に関する研究2018

    • Author(s)
      山田和輝・呉海雲・村田政隆・松本陽斗・大貫等・遠藤英明
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 魚類のストレス応答可視化のためのLED通信・点灯一体型バイオセンサシステムの試作に関する研究2018

    • Author(s)
      平井愛美・呉海雲・村田政隆・松本陽斗・大貫等・遠藤英明
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 魚類のためのコルチゾル連続測定用フロー式バイオセンサシステムの試作2018

    • Author(s)
      呉海雲・吉田兼也・大貫等・遠藤英明
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Development of a immunosensor system using enzyme reaction for detecting oocyte maturation-inducing hormone in fish2017

    • Author(s)
      Y. Saito, H. Wu, H. Ohnuki, H. Endo
    • Organizer
      International Symposium “Fisheries Science for Future Generations”, Tokyo, Japan
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of novel glucose biosensor for fish using self-assembled monolayer2017

    • Author(s)
      Y. Fujii, K. Yamada, H. Wu, H. Ohnuki, M. Murata, K. Hibi, H. Endo
    • Organizer
      International Symposium “Fisheries Science for Future Generations”, Tokyo, Japan
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of a novel immunosenser system for fish sex determination.2017

    • Author(s)
      T. Sakurai, H. Wu, G. Yoshizaki, H. Ohnuki, H. Endo
    • Organizer
      International Symposium “Fisheries Science for Future Generations”, Tokyo, Japan
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of LED-color-switching type biosensor for the visualization of fish stress response2017

    • Author(s)
      H. Endo, H. Wu, R. Shinoda, M. Murata, H. Ohnuki:
    • Organizer
      BBMEC 12: Workshop on Biosensor & Bioanalytical Microtechniques in Environment, Food & Clinical Analysis, Rome, Italy
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of novel biosensor system for fish stress monitoring using self-assembled monolayer2017

    • Author(s)
      H. Wu, Y. Fujii, H. Ohnuki, M. Murata, H. Endo
    • Organizer
      BBMEC 12: Workshop on Biosensor & Bioanalytical Microtechniques in Environment, Food & Clinical Analysis, Rome, Italy
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] New design of label-free human immunoglobulin G impedance biosensor with micro-gap parallel plate electrodes2017

    • Author(s)
      Y. Kusaka, H. Ohnuki, H. Wu, H. Endo, D. Tsuya
    • Organizer
      BBMEC 12: Workshop on Biosensor & Bioanalytical Microtechniques in Environment, Food & Clinical Analysis, Rome, Italy
    • Related Report
      2017 Annual Research Report
  • [Book] 酵素トランスデューサーと酵素技術展開(バイオセンサによる魚類のストレス応答の可視化)2020

    • Author(s)
      遠藤英明,呉海云(分担執筆)(三林浩二監修)
    • Total Pages
      300
    • Publisher
      シーエムシー出版
    • ISBN
      9784781314884
    • Related Report
      2020 Annual Research Report
  • [Book] バイオセンサによる魚類のストレス応答の可視化~酵素トランスデューサーと酵素技術展開(三林浩二監修)2020

    • Author(s)
      遠藤英明,呉海云(分担執筆)
    • Total Pages
      304
    • Publisher
      シーエムシー出版書籍
    • ISBN
      9784781314884
    • Related Report
      2019 Annual Research Report

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Published: 2017-04-28   Modified: 2022-01-27  

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