| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2020: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Outline of Final Research Achievements |
A new system for fish seedling production through germline chimera will be able to breed tuna species which is believed to be difficult to perform a conventional selective breeding. This system is based on fish surrogate propagation that produce donor-derived gametes through host fish by transplanting donor germ cells. In this study, we aimed to develop methods for inducing sterilized host in small tuna species, kawakawa, Euthynnus affinis. First, we confirmed that genome editing using TALEN and CRISPR/Cas9 was able to edit body color related genes in kawakawa. Then, we developed methods for inducing a complete or partial sterilization by blocking translation of dead end gene or induction of exogenous gene expression in PGC as a method without gene-modified.
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