Studies on environment control for and the mechanism underlying its effects on plant-based protein production

• Project/Area Number: 17H03893
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Agricultural environmental engineering/Agricultural information engineering
• Research Institution: The University of Tokyo
• Principal Investigator: MATSUDA Ryo 東京大学, 大学院農学生命科学研究科(農学部), 准教授 (20547228)
• Co-Investigator(Kenkyū-buntansha): 富士原 和宏 東京大学, 大学院農学生命科学研究科(農学部), 教授 (30211535)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2017: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: 環境制御 / バイオ医薬品 / 植物工場 / 一過性遺伝子発現 / ベンサミアナタバコ / インフルエンザワクチン / ヘマグルチニン / 温室 / 人工光型植物工場 / 遺伝子発現 / 小胞体ストレス / 植物利用型有用タンパク質生産 / 二酸化炭素施用 / 農業工学 / 環境 / 植物 / バイオテクノロジー / 蛋白質

Outline of Final Research Achievements

Effects of environmental conditions before and after gene transfer and the mechanism underlying the effects in plant-based protein production with transient gene expression system were investigated. It was found that high light intensity and high CO2 concentration after gene transfer were not necessarily effective in hemagglutinin (HA) prodution and that PPFD and temperature control before gene transfer is important for HA production after gene transfer. It was also suggested that ER stress can be involved in leaf necrosis observed after gene transfer under a specific temperature condition.

Academic Significance and Societal Importance of the Research Achievements

一過性遺伝子発現法を用いた植物利用型有用タンパク質生産において，遺伝子導入前後の環境制御の効果と，その効果の合理的解釈に関する知見が得られた。また，遺伝子発現の定量解析により，環境制御の効果をもたらす生理的メカニズムの一端が明らかになった。いずれも，実用的な植物利用型有用タンパク質生産において有用な知見となりうると考える。

Research Products

• [Int'l Joint Research] ルイビル大学(米国)
• [Int'l Joint Research] ルイビル大学(米国)
• [Journal Article] Effects of environmental conditions before gene transfer on the amount of influenza hemagglutinin transiently expressed in <I>Nicotiana benthamiana</I> leaves (2019)
  • Author(s): Matsuda, R., A. Ueno, K. Fujiwara
  • Journal Title: Journal of Agricultural Meteorology Volume: 75 Issue: 3 Pages: 129-136
  • DOI: 10.2480/agrmet.D-18-00050
  • NAID: 130007677014
  • ISSN: 0021-8588, 1881-0136
  • Peer Reviewed / Open Access
• [Journal Article] Gas exchange rates decrease and leaf temperature increases in Nicotiana benthamiana leaves transiently overexpressing hemagglutinin in an Agrobacterium-assisted viral vector system (2018)
  • Author(s): Matsuda, R., A. Ueno, H. Nakaigawa, K. Fujiwara
  • Journal Title: Frontiers in Plant Science Volume: 9 Pages: 1315-1315
  • DOI: 10.3389/fpls.2018.01315
  • Peer Reviewed / Open Access
• [Journal Article] 植物を利用した医薬用タンパク質生産のための環境調節 (2018)
  • Author(s): 松田 怜・藤内直道
  • Journal Title: 関東の農業気象 Volume: 44 Pages: 6-11
  • NAID: 40021691857
  • Open Access
• [Journal Article] Agroinfiltration of leaves for deconstructed viral vector-based transient gene expression: infiltrated leaf area affects recombinant hemagglutinin yield (2018)
  • Author(s): Matsuda, R., T. Kushibiki, N. Fujiuchi, K. Fujiwara
  • Journal Title: Horticulture, Environment, and Biotechnology Volume: 印刷中
  • Peer Reviewed
• [Presentation] Engineering, physiology, and biotechnology: research efforts toward next-generation controlled environment agriculture (2019)
  • Author(s): Matsuda, R.
  • Organizer: American Society for Horticultural Science 2019 Annual Meeting
  • Int'l Joint Research / Invited
• [Presentation] Plant-based influenza hemagglutinin production under controlled environment: efforts toward optimizing plant growth conditions for a transient gene expression system (2019)
  • Author(s): Matsuda, R.
  • Organizer: PBVAB 2019
  • Int'l Joint Research
• [Presentation] The effect of temperature post inoculation on hemagglutinin transcription in Nicotiana benthamiana leaves in Agrobacterium-mediated transient gene expression (2019)
  • Author(s): Atsumi, K., Y. Oh, N. Matoba, K. Fujiwara, R. Matsuda
  • Organizer: PBVAB 2019
  • Int'l Joint Research
• [Presentation] 一過性遺伝子発現法における遺伝子導入前の栽培環境が葉内ワクチン含量に及ぼす影響 (2019)
  • Author(s): 松田 怜・上野彰大・富士原和宏
  • Organizer: 日本農業気象学会2019年全国大会
• [Presentation] Environment control for plant-based biopharmaceutical protein production using transient gene expression technology (2018)
  • Author(s): Matsuda, R.
  • Organizer: The 3rd Trilateral (Korea-China-Japan) Research Association of Plant Biotechnology Symposium
  • Int'l Joint Research / Invited
• [Presentation] 人工光型植物工場は必要なのか？ (2018)
  • Author(s): 松田 怜
  • Organizer: 日本農業気象学会75周年記念大会
• [Presentation] Environmental control for recombinant protein production in plants using transient gene expression technology (2017)
  • Author(s): Matsuda, R., N. Fujiuchi, K. Fujiwara
  • Organizer: GreenSys 2017
  • Int'l Joint Research
• [Presentation] Environment control for recombinant protein production in Nicotiana benthamiana plants using a transient gene expression system (2017)
  • Author(s): Matsuda, R.
  • Organizer: PMPAsia 2017
  • Int'l Joint Research