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Responses of gene expressions in rice plants to micro-meteorological conditions; their physiological role in absorption of water and nutrients from roots

Research Project

Project/Area Number 17H03896
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Agricultural environmental engineering/Agricultural information engineering
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

KUWAGATA Tsuneo  国立研究開発法人農業・食品産業技術総合研究機構, 農業環境変動研究センター, ユニット長 (90195602)

Co-Investigator(Kenkyū-buntansha) 近藤 始彦  名古屋大学, 生命農学研究科, 教授 (00355538)
伊川 浩樹  国立研究開発法人農業・食品産業技術総合研究機構, 農業環境変動研究センター, 主任研究員 (10754393)
杉浦 大輔  名古屋大学, 生命農学研究科, 助教 (50713913)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2017: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Keywordsアクアポリン / イネ / 環境応答 / 群落微気象 / 蒸散 / 植物生理 / トランスクリプトーム / 農業生産環境
Outline of Final Research Achievements

We experimentally investigated how micro-meteorological conditions influence expression levels of the functional genes which contribute to absorption of water and nutrients by the rice crops, and examined the physiological roles of the environmental responses of these genes to the growth of rice. The experimental results indicated that the expression levels of many kinds of aquaporin genes both in roots and leaves increased with the transpirational demand during the all growth stages, and that expression level of about half of all genes in the rice leaves had a positive (or negative) correlation with transpiration in the early ripening period. Finally, we demonstrated that the environmental response of functional genes to day-to-day variation in micro-meteorological conditions could play important roles in several eco-physiological processes, such as physiological response of roots to decrease in soil water content and change in water use efficiency with atmospheric CO2 concentration.

Academic Significance and Societal Importance of the Research Achievements

植物の生育は多数の遺伝子によって調整されているが、植物が生育する野外では、光環境や気温・湿度、地温などの複数の環境がさまざまな時間スケールで複雑に変化する。野外で生育してるイネを対象として、水や養分の輸送を担う各種遺伝子の状態と生育状態の変化を調べたところ、イネは日々の蒸散要求量や土壌水分などの微気象環境の変化に応じて、それら遺伝子の発現量をダイナミックに変化させ、自らの水輸送や養分吸収、光合成などの機能を柔軟に調節している可能性が示された。これは植物の生存戦略において重要な調節機能であり、気候変動下における作物の安定生産技術を構築する上で役立つ基礎的な知見である。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (26 results)

All 2020 2019 2018 2017

All Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 7 results) Presentation (16 results) (of which Int'l Joint Research: 9 results)

  • [Journal Article] A Literature Review on Vegetation-Atmosphere Interaction Research for Carbon Cycle and Energy Balance in Terrestrial Ecosystems2019

    • Author(s)
      IKAWA Hiroki
    • Journal Title

      低温科学

      Volume: 77 Pages: 1-15

    • DOI

      10.14943/lowtemsci.77.1

    • NAID

      120006628718

    • ISSN
      1880-7593
    • Year and Date
      2019-03-20
    • Related Report
      2018 Annual Research Report
    • Open Access
  • [Journal Article] Micro-climate model for a rice paddy field and its application to agriculture2019

    • Author(s)
      桑形恒男,伊川浩樹,丸山篤志,小野圭介,吉本真由美,石田祐宣,渡辺力
    • Journal Title

      低温科学

      Volume: 77 Pages: 125-136

    • DOI

      10.14943/lowtemsci.77.125

    • NAID

      120006628730

    • ISSN
      1880-7593
    • Year and Date
      2019-03-20
    • Related Report
      2018 Annual Research Report
    • Open Access
  • [Journal Article] Simulation of paddy water temperature for advanced water management on agricultural lands2019

    • Author(s)
      丸山篤志,石田祐宣,桑形恒男,渡辺力
    • Journal Title

      低温科学

      Volume: 77 Pages: 137-144

    • DOI

      10.14943/lowtemsci.77.137

    • NAID

      120006628731

    • ISSN
      1880-7593
    • Year and Date
      2019-03-20
    • Related Report
      2018 Annual Research Report
    • Open Access
  • [Journal Article] High mesophyll conductance in the high-yielding rice cultivar Takanari quantified with the combined gas exchange and chlorophyll fluorescence measurements under free-air CO2 enrichment2019

    • Author(s)
      Ikawa, H., Sakai, H., Chen, C.P., Soong, T.H., Yonemura, S., Taniguchi, Y.,Yoshimoto, M., Tokida, T., Zhang, G., Kuwagata, T., Nakamura, H., Avenson,T., and Hasegawa, T.
    • Journal Title

      Plant Production Science

      Volume: 22 Issue: 3 Pages: 395-406

    • DOI

      10.1080/1343943x.2019.1626253

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effective methods for practical application of gene expression analysis in field-grown rice roots2018

    • Author(s)
      Maya Matsunami, Hidehiro Hayashi, Yoko Tominaga, Yoshiaki Nagamura, Mari Murai-Hatano, Junko Ishikawa-Sakurai, Tsuneo Kuwagata
    • Journal Title

      Plant and Soil

      Volume: 433 Issue: 1-2 Pages: 173-187

    • DOI

      10.1007/s11104-018-3834-z

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Influence of local land cover on meteorological conditions in farmland: Case study of a rice paddy field near Tsukuba City, Japan2018

    • Author(s)
      KUWAGATA Tsuneo、HAGINOYA Shigenori、ONO Keisuke、ISHIGOOKA Yasushi、MIYATA Akira
    • Journal Title

      Journal of Agricultural Meteorology

      Volume: 74 Issue: 4 Pages: 140-153

    • DOI

      10.2480/agrmet.D-18-00018

    • NAID

      130007496544

    • ISSN
      0021-8588, 1881-0136
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Increasing canopy photosynthesis in rice can be achieved without a large increase in water use-a model based on free-air CO2 enrichment2018

    • Author(s)
      Ikawa, H., Chen, C.P., Sikma, M., Yoshimoto, M., Sakai, H., Tokida, T., Usui, Y., Nakamura, H., Ono, K., Maruyama, M., Watanabe, T., Kuwagata, T., and Hasegawa, T.
    • Journal Title

      Global Change Biology

      Volume: 24 Issue: 3 Pages: 1321-1341

    • DOI

      10.1111/gcb.13981

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Taking account of water temperature effects on phenology improves the estimation of rice heading dates: Evidence from 758 field observations across Japan2017

    • Author(s)
      Fukui S., Ishigooka Y., Kuwagata T., Kondo M., and Hasegawa T.
    • Journal Title

      Journal of Agricultural Meteorology

      Volume: 73 Issue: 3 Pages: 84-91

    • DOI

      10.2480/agrmet.D-16-00018

    • NAID

      130005797420

    • ISSN
      0021-8588, 1881-0136
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evapotranspiration in a rice paddy field over 13 crop years2017

    • Author(s)
      Ikawa H., Ono K., Mano M., Kobayashi K., Takimoto T., Kuwagata T., and Miyata A.
    • Journal Title

      Journal of Agricultural Meteorology

      Volume: 73 Issue: 3 Pages: 109-118

    • DOI

      10.2480/agrmet.D-16-00011

    • NAID

      130005797425

    • ISSN
      0021-8588, 1881-0136
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] イネにおけるアクアポリンの機能と環境応答2017

    • Author(s)
      石川(櫻井)淳子, 羽田野麻理, 林秀洋, 松波麻耶, 桑形恒男
    • Journal Title

      根の研究

      Volume: 26(3) Pages: 39-55

    • NAID

      130006072896

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] Characteristics and monitoring of local climate in rice paddy area of Japan2020

    • Author(s)
      Tsuneo Kuwagata, Yasushi Ishigooka Shigenori Haginoya Hiroki Ikawa, Keisuke Ono, Mayumi Yoshimoto, Minehiko Fukuoka, Motoki Nishimori, Toshihiro Hasegawa, Yasuhiro Usui, and Akira Miyata
    • Organizer
      49th Asia Pacific Advanced Network (APAN) Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 蒸散要求量はイネのトランスクリプトームを制御する重要な微気象要因である2020

    • Author(s)
      桑形恒男、石田祐宣、羽田野(村井)麻理、松波麻耶、照井慎吾、永野惇
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 土壌の酸化還元状態に対する応答性のイネ品種間差異の解明2020

    • Author(s)
      平松秀一、杉浦大輔、永野惇、近藤始彦
    • Organizer
      日本作物学会第249回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Characteristics of local climate in rice paddy area of Japan2019

    • Author(s)
      Tsuneo Kuwagata, Yasushi Ishigooka Shigenori Haginoya Hiroki Ikawa, Keisuke Ono, Mayumi Yoshimoto, Minehiko Fukuoka, Motoki Nishimori, Toshihiro Hasegawa, Yasuhiro Usui, and Akira Miyata
    • Organizer
      AgMIP-East Asia and Climate Smart Agriculture Symposium & The 6th Symposium on the Development and Application of Agricultural System Models in China
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Mining physiological and phenological traits to increase water use efficiency of a high-yielding rice cultivar using a crop growth model2019

    • Author(s)
      Hiroki Ikawa, Keisuke Ono, Akira Miyata, Yasushi Ishigooka, Tsuneo Kuwagata, Tsutomu Watanabe, Motoki Nishimori, Hidemitsu Sakai, Charles P. Chen, Mayumi Yoshimoto, Takeshi Tokida, Hirofumi Nakamura, Hitomi Wakatsuki, and Toshihiro Hasegawa
    • Organizer
      gMIP-East Asia and Climate Smart Agriculture Symposium & The 6th Symposium on the Development and Application of Agricultural System Models in China
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Rice quality analysis using the Crop Survey database of Japan for climate impact assessment2019

    • Author(s)
      Nishimori, M., Ishiooka, Y., Kuwagata T., Wakatsuki, H., and Hasegawa, T.
    • Organizer
      3rd Agriculture and Climate Change Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Field-scale productivity and water use of a high-yielding rice cultivar2019

    • Author(s)
      Ono, K., and Ikawa, H.
    • Organizer
      International Symposium on Agricultural Meteorology 2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Simple physical-based model of transpiration and water uptake of plants for physio-ecological study2019

    • Author(s)
      桑形恒男、伊川浩樹
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 農耕地における土壌温度・水分推定システムのプロトタイピング2019

    • Author(s)
      滝本貴弘、高田裕介、桑形恒男
    • Organizer
      日本農業気象学会2019年全国大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Modeling of energy exchange and water temperature for paddy fields with variable water depth and surface roughness2018

    • Author(s)
      Maruyama, A. Nemoto, M., Hamasaku, T., Ishida, S., and Kuwagata, T.
    • Organizer
      AGU Fall Meeting 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 還元的根圏環境に対する応答性のイネ品種間差2018

    • Author(s)
      平松秀一、杉浦大輔、近藤始彦
    • Organizer
      日本作物学会第246回講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Rice production, water use, and atmospheric feedbacks2018

    • Author(s)
      Ikawa, H.
    • Organizer
      International Symposium on Agricultural Meteorology 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Influence of local land cover to the meteorological conditions in farmland -Case Study for rice paddy field near Tsukuba City in Japan-2018

    • Author(s)
      Kuwagata, T., Haginoya, S., Ono, K., and Ishigooka, Y.
    • Organizer
      International Symposium on Agricultural Meteorology 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Differences of canopy environment and stomatal conductance in sugar beet (Beta vulgaris)2018

    • Author(s)
      Usui, Y., Hirota, T., Kuwagata, T., Taniguchi, K., and Hirafuji, M.
    • Organizer
      International Symposium on Agricultural Meteorology 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 野外群落環境における水稲根の水と窒素の吸収に関連する遺伝子の発現動態2018

    • Author(s)
      松波麻耶,羽田野麻理,林秀洋,桑形恒男,石川淳子
    • Organizer
      日本作物学会第245回講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 異なる水稲品種による群落が大気の熱環境に及ぼす影響2017

    • Author(s)
      伊川浩樹,桑形恒男
    • Organizer
      日本気象学会2017年度秋季大会
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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