Regulation of flavivirus infection by virus-derived long non-coding RNA
Project/Area Number |
17H03910
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Veterinary medical science
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Research Institution | Nagasaki University (2019) Hokkaido University (2017-2018) |
Principal Investigator |
YOSHII Kentaro 長崎大学, 感染症共同研究拠点, 教授 (50421988)
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Co-Investigator(Kenkyū-buntansha) |
田仲 哲也 鹿児島大学, 農水産獣医学域獣医学系, 教授 (00322842)
小林 進太郎 北海道大学, 獣医学研究院, 助教 (00634205)
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Project Period (FY) |
2017-04-01 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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Keywords | フラビウイルス / ノンコーディングRNA / 節足動物 / 人獣共通感染症 |
Outline of Final Research Achievements |
The 3'-untranslated region (3'-UTR) of tick-borne encephalitis virus (TBEV) plays an important role in the viral pathogenesis. In this study, we investigated detailed mechanisms of virus-derived long non-coding RNA (lncRNA) from the 3'-UTR of TBEV in TBEV infection. Deletions in the 3'-UTR increased virulence in a mouse model and affected the production of virus-derived lncRNA. We identified host proteins interacting the 3'-UTR of TBEV RNA in mammalian and tick cells, and revealed that the interaction affected viral replication mechanisms. These insights are useful for understanding virus transmission and pathogenic mechanism of flavivirus.
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Academic Significance and Societal Importance of the Research Achievements |
節足動物媒介性フラビウイルスは、媒介動物であるダニや蚊においては、病原性を示すことなく持続感染が成立しているのに対し、脊椎動物に感染した場合、宿主に対して様々な病原性を示すが、これらの機序には不明な点が多く、有効な治療法や感染の制御法の開発には至っていない。本研究ではウイルスのコードする蛋白質ではなく、蛋白をコードしないRNAに着目し解析することにより、これらのRNAが宿主の応答を制御することによって、感染に影響を与えていることを示した。これらの成果は、フラビウイルスの感染現象の全容解明の基盤となるとともに、将来的な流行防止対策等にも応用可能な発展性を有していると考えられる。
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Report
(4 results)
Research Products
(152 results)
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[Journal Article] Detection of a novel tick-borne flavivirus and its serological surveillance.2019
Author(s)
Shimoda H, Hayasaka D, Yoshii K, Yokoyama M, Suzuki K, Kodera Y, Takeda T, Mizuno J, Noguchi K, Yonemitsu K, Minami S, Kuwata R, Takano A, Maeda K.
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Journal Title
Ticks Tick Borne Dis.
Volume: -
Issue: 4
Pages: 742-748
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Peroxiredoxins are important for the regulation of hydrogen peroxide concentrations in ticks and tick cell line2018
Author(s)
Kusakisako, K., Hernandez, E. P., Talactac, M. R., Yoshii, K., Umemiya-Shirafuji, R., Fujisaki, K. and Tanaka, T.
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Journal Title
Ticks Tick Borne Dis.
Volume: In press
Issue: 4
Pages: 870-881
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Induction of intracellular ferritin expression in embryo-derived Ixodes scapularis cell line (ISE6)2018
Author(s)
Hernandez, E. P., Kusakisako, K., Talactac, M. R., Galay, R. L., Yoshii, K. and Tanaka, T.
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Journal Title
Sci. Rep.
Volume: 8(1)
Issue: 1
Pages: 16566-16566
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] High-throughput neutralization assay for multiple flaviviruses based on single-round infectious particles using dengue virus type 1 reporter replicon.2018
Author(s)
Matsuda M, Yamanaka A, Yato K, Yoshii K, Watashi K, Aizaki H, Konishi E, Takasaki T, Kato T, Muramatsu M, Wakita T, Suzuki R
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Journal Title
Sci Rep
Volume: 8
Issue: 1
Pages: 16624-16624
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Characterization and antiviral activity of a newly identified defensin-like peptide, HEdefensin, in the hard tick Haemaphysalis longicornis2017
Author(s)
Talactac, M. R., Yada, Y., Yoshii, K., Hernandez, E. P., Kusakisako, K., Maeda, H., Galay, R. L., Fujisaki, K., Mochizuki, M. and Tanaka, T.
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Journal Title
Dev. Comp. Immunol.
Volume: 68
Pages: 98-107
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Synchronous Langat virus infection of Haemaphysalis longicornis using anal pore microinjection2017
Author(s)
Talactac, M. R., Yoshii, K., Hernandez, E. P., Kusakisako, K., Galay, R. L., Fujisaki, K., Mochizuki, M. and Tanaka, T.
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Journal Title
Viruses
Volume: 9(7)
Issue: 7
Pages: 189-189
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Escape of tick-borne flavivirus from 2’-C-methylated nucleoside antivirals is mediated by a single conservative mutation in NS5 that has a dramatic effect on viral fitness2017
Author(s)
Eyer L, Kondo H, Zouharov D, Hirano M, Valds J, Muto M, Kastl T, Kobayashi S, Haviernik J, Igarashi M, Kariwa H, Vaculovicova M, Cerny J, Kizek R, Krger A, Lienenklaus S, Dejmek M, Nencka R, Palus M, Salat J, Clercq ED, Yoshii K, Ruzek D
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Journal Title
J Virol
Volume: 91
Issue: 21
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Putative RNA viral sequences detected in an Ixodes scapularis-derived cell line.2016
Author(s)
Nakao R, Matsuno K, Qiu Y, Maruyama J, Eguchi N, Nao N, Kajihara M, Yoshii K, Sawa H, Takada A, Sugimoto C.
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Journal Title
Ticks Tick Borne Dis.
Volume: 8
Issue: 1
Pages: 103-111
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] A novel tick-borne flavivirus discovered in Zambia is closely related to Ngoye virus, representing a distinct lineage within the genus Flavivirus.2019
Author(s)
Hayato Harima, Shiho Torii, Yongjin Qiu, Masahiro Kajihara, Yoshiki Eto, Yasuko Orba, Yuki Eshita, Barnard M Hang' ombe, Kentaro Yoshii, Martin Simuunza, Hirofumi Sawa.
Organizer
第67回日本ウイルス学会学術集会
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[Presentation] Characterization and antiviral activity of a newly identified defensin-like peptide, HEdefensin, in the hard tick Haemaphysalis longicornis2017
Author(s)
Melbourne Rio Talactac, Yurika Yada, Kentaro Yoshii, Emmanuel Pacia Hernandez, Kodai Kusakisako, Hiroki Maeda, Kozo Fujisaki, Masami Mochizuki, Tetsuya Tanaka
Organizer
第160回日本獣医学会学術集会
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