Comprehensive analyses in components of Toxoplasma dormant parasites.

• Project/Area Number: 17H03913
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Tohoku University (2018-2020); Obihiro University of Agriculture and Veterinary Medicine (2017)
• Principal Investigator: KATO Kentaro 東北大学, 農学研究科, 教授 (30401178)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000); Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2018: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: トキソプラズマ / 原虫 / 潜伏感染 / 感染症 / 抗原虫薬 / 人獣共通感染症

Outline of Final Research Achievements

This research focuses on molecular mechanism of Toxoplasma latent infection, which has not been studied yet. By the characterization of cyst, a dormant parasite, which is a source of infection, we performed to select parasite factors enough which can be targeted for anti-parasite drugs. At first, we detected the parasite factors constituting cyst wall comprehensively in the dormant Toxoplasma-infected cells. Moreover, we characterized the detected parasite factors in the latent infection.

Academic Significance and Societal Importance of the Research Achievements

本研究では、トキソプラズマの潜伏感染虫体の性状解析を行ったが、ここで得られた知見は休眠型虫体を持つ三日熱マラリア等の他の原虫のライフサイクルの理解とその感染阻止につながる。本研究で同定した潜伏感染制御因子は、トキソプラズマの潜伏感染阻止薬の開発に結び付くことが期待できる。このような新規の薬剤は、食肉となる家畜の感染阻止、食品価値の向上に役立つだけでなく、近年産婦人科領域で問題となっている妊婦でのトキソプラズマ初感染時の処方薬としての利用が期待できる。

Research Products

• [Int'l Joint Research] 内モンゴル民族大学(中国)
• [Int'l Joint Research] シェレバングラ農科大学(バングラデシュ)
• [Int'l Joint Research] LANDMARK UNIVERSITY(ナイジェリア)
• [Int'l Joint Research] 内モンゴル民族大学(中国)
• [Int'l Joint Research] LANDMARK UNIVERSITY(ナイジェリア)
• [Int'l Joint Research] アルベルト・アインシュタイン医学校(米国)
• [Journal Article] Screening of a library of traditional Chinese medicines to identify compounds and extracts which inhibit <i>Toxoplasma gondii</i> growth (2020)
  • Author(s): SAITO Harunobu、MURATA Yuho、NONAKA Motohiro、KATO Kentaro
  • Journal Title: Journal of Veterinary Medical Science Volume: 82 Issue: 2 Pages: 184-187
  • DOI: 10.1292/jvms.19-0241
  • NAID: 130007799363
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Tree of motility : A proposed history of motility systems in the tree of life (2020)
  • Author(s): M Miyata, R C Robinson, T QP Uyeda, Y Fukumori, S Fukushima, S Haruta, M Homma, K Inaba, M Ito, C Kaito, K Kato, TKenri, Y Kinosita, S Kojima, T Minamino, H Mori, S Nakamura, D Nakane, K Nakayama, M Nishiyama, S Shibata, K Shimabukuro, M Tamakoshi, A Taoka, Y Tashiro, ITulum, HiWada, K Wakabayashi
  • Journal Title: Genes to Cells Volume: 25 Issue: 1 Pages: 6-21
  • DOI: 10.1111/gtc.12737
  • NAID: 130007881664
  • URL: https://ocu-omu.repo.nii.ac.jp/records/2019864
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Repurposing existing drugs: identification of irreversible IMPDH inhibitors by high-throughput screening (2019)
  • Author(s): Sarwono Albertus Eka Yudistira、Mitsuhashi Shinya、Kabir Mohammad Hazzaz Bin、Shigetomi Kengo、Okada Tadashi、Ohsaka Fumina、Otsuguro Satoko、Maenaka Katsumi、Igarashi Makoto、Kato Kentaro、Ubukata Makoto
  • Journal Title: Journal of Enzyme Inhibition and Medicinal Chemistry Volume: 34 Issue: 1 Pages: 171-178
  • DOI: 10.1080/14756366.2018.1540474
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Nanoparticles show potential to retard bradyzoites in vitro formation of Toxoplasma gondii (2019)
  • Author(s): Adeyemi Oluyomi Stephen、Murata Yuho、Sugi Tatsuki、Han Yongmei、Kato Kentaro
  • Journal Title: Folia Parasitologica Volume: 66
  • DOI: 10.14411/fp.2019.001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] How does <i>Toxoplama gondii</i> invade host cells? (2018)
  • Author(s): KATO Kentaro
  • Journal Title: Journal of Veterinary Medical Science Volume: 80 Issue: 11 Pages: 1702-1706
  • DOI: 10.1292/jvms.18-0344
  • NAID: 130007521222
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Metal nanoparticles restrict the growth of protozoan parasites (2018)
  • Author(s): Adeyemi Oluyomi Stephen、Molefe Nthatisi Innocentia、Awakan Oluwakemi Josephine、Nwonuma Charles Obiora、Alejolowo Omokolade Oluwaseyi、Olaolu Tomilola、Maimako Rotdelmwa Filibus、Suganuma Keisuke、Han Yongmei、Kato Kentaro
  • Journal Title: Artificial Cells, Nanomedicine, and Biotechnology Volume: 46 Issue: sup3 Pages: 86-94
  • DOI: 10.1080/21691401.2018.1489267
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Exploring Amino Acid-Capped Nanoparticles for Selective Anti-Parasitic Action and Improved Host Biocompatibility (2018)
  • Author(s): Adeyemi Oluyomi Stephen、Murata Yuho、Sugi Tatsuki、Han Yongmei、Kato Kentaro
  • Journal Title: Journal of Biomedical Nanotechnology Volume: 14 Issue: 5 Pages: 847-867
  • DOI: 10.1166/jbn.2018.2544
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Toxoplasma gondii RON4 binds to heparan sulfate on the host cell surface (2018)
  • Author(s): Takemae, H., Kobayashi, K., Sugi, T., Han, Y., Gong, H., Ishiwa, A., Recuenco, F. C., Murakoshi, F., Takano, R., Murata, Y., Nagamune, K., Horimoto, T., Akashi, H., Kato, K.
  • Journal Title: Parasitol. Int. Volume: 67 Issue: 2 Pages: 123-130
  • DOI: 10.1016/j.parint.2017.10.008
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Screening of chemical compound libraries identified new anti-Toxoplasma gondii agents (2017)
  • Author(s): Adeyemi Oluyomi Stephen、Sugi Tatsuki、Han Yongmei、Kato Kentaro
  • Journal Title: Parasitology Research Volume: 117 Issue: 2 Pages: 355-363
  • DOI: 10.1007/s00436-017-5698-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Modulation of host HIF-1α activity and the tryptophan pathway contributes to the anti- Toxoplasma gondii potential of nanoparticles (2017)
  • Author(s): Adeyemi Oluyomi Stephen、Murata Yuho、Sugi Tatsuki、Han Yongmei、Kato Kentaro
  • Journal Title: Biochemistry and Biophysics Reports Volume: 11 Pages: 84-92
  • DOI: 10.1016/j.bbrep.2017.07.004
  • NAID: 120006390983
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of compounds that suppress Toxoplasma gondii tachyzoites and bradyzoites (2017)
  • Author(s): Murata Yuho、Sugi Tatsuki、Weiss Louis M.、Kato Kentaro
  • Journal Title: PLOS ONE Volume: 12 Issue: 6 Pages: e0178203-e0178203
  • DOI: 10.1371/journal.pone.0178203
  • NAID: 120006391021
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] ヒト脳細胞におけるトキソプラズマのステージ変換機構の解明 (2019)
  • Author(s): 伴戸寛徳、加藤健太郎
  • Organizer: 第 27 回分子寄生虫学ワークショップ
• [Presentation] ヒト脳細胞における宿主-トキソプラズマ間相互作用の解明 (2019)
  • Author(s): 伴戸寛徳、山本雅裕、加藤健太郎
  • Organizer: 第162回日本獣医学会学術集会
• [Presentation] 抗トキソプラズマ作用を持つプロテインキナーゼ阻害剤のスクリーニング (2019)
  • Author(s): 韓 永梅、Oluyomi Stephen Adeyemi、加藤健太郎
  • Organizer: 第162回日本獣医学会学術集会
• [Presentation] 原虫の特異的な宿主細胞侵入マシナリーと薬剤ターゲットとしての可能性 (2019)
  • Author(s): 加藤健太郎
  • Organizer: 第92回日本生化学会大会
  • Invited
• [Presentation] トキソプラズマのChitinase Like Protein 1の機能解析 (2019)
  • Author(s): 齋藤晴伸、韓 永梅、村田優穂、加藤健太郎
  • Organizer: 第88回日本寄生虫学会大会（長崎大学、長崎）
• [Presentation] In vitro target inhibition of Toxoplasma gondii growth using tryptophan-capped nanoparticles (2019)
  • Author(s): 韓 永梅, Oluyomi Stephen Adeyemi, 加藤 健太郎
  • Organizer: 第88回日本寄生虫学会大会（長崎大学、長崎）
• [Presentation] ヒト脳細胞における宿主-トキソプラズマ間相互作用の解明 (2019)
  • Author(s): Adeyemi Oluyomi Stephen、加藤 健太郎
  • Organizer: 第88回日本寄生虫学会大会（長崎大学、長崎）
• [Presentation] Amino acids-capped nanoparticles show multiple fold anti-Toxoplasma gondii action (2018)
  • Author(s): ADEYEMI Oluyomi Stephen、加藤健太郎
  • Organizer: 第161回日本獣医学会（つくば国際会議場）
• [Presentation] トキソプラズマ原虫の増殖と侵入を抑制する薬剤のスクリーニング (2018)
  • Author(s): 韓永梅、Oluyomi Stephen Adeyemi、加藤健太郎
  • Organizer: 第161回日本獣医学会（つくば国際会議場）
• [Presentation] トキソプラズマ原虫の宿主細胞侵入におけるRON4とヘパラン硫酸の相互作用 (2018)
  • Author(s): 加藤健太郎
  • Organizer: 生体運動研究合同班会議
• [Presentation] New imidazole derivatives active against Toxoplasma gondii in vitro (2018)
  • Author(s): Oluyomi Stephen Adeyemi、加藤健太郎
  • Organizer: 第87回日本寄生虫学会
• [Presentation] トキソプラズマ原虫の増殖を抑制するプロテインキナーゼ阻害剤ライブラリーのスクリーニング (2018)
  • Author(s): 韓永梅、Oluyomi Stephen Adeyemi、加藤健太郎
  • Organizer: 第87回日本寄生虫学会
• [Presentation] トキソプラズマ原虫のChitinase Like Protein 1 は潜伏虫体の再活性化に関与する (2017)
  • Author(s): 村田優穂、高野量、五十嵐慎、杉達紀、加藤健太郎
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] Exploring amino acid modification of nanoparticle surface for selective anti-parasite action and enhanced biocompatibility (2017)
  • Author(s): Oluyomi Stephen Adeyemi、加藤健太郎
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] トキソプラズマ原虫の潜伏再活性化因子の機能解析 (2017)
  • Author(s): 村田優穂、高野量、五十嵐慎、杉達紀、加藤健太郎
  • Organizer: 第160回日本獣医学会
• [Presentation] Exploring amino acid modification of nanoparticles for selective anti-parasite action and enhanced biocompatibility (2017)
  • Author(s): Oluyomi Adeyemi、加藤 健太郎
  • Organizer: 第160回日本獣医学会
  • Invited
• [Presentation] トキソプラズマ症の病態発現機構と対策技術に関する研究 (2017)
  • Author(s): 加藤健太郎
  • Organizer: 第160回日本獣医学会
  • Invited
• [Presentation] トキソプラズマ症の潜伏再活性化機構の解析 (2017)
  • Author(s): 加藤健太郎、村田優穂、高野量、五十嵐慎、杉達紀
  • Organizer: グローバルヘルス合同大会2017
• [Remarks] 東北大学大学院農学研究科動物環境システム学分野のホームページ
  • URL: https://www.agri.tohoku.ac.jp/health/index.html
• [Patent(Industrial Property Rights)] 防除用製剤、並びに土壌処理方法 (2019)
  • Inventor(s): 加藤健太郎、大川和久、升水紀郎
  • Industrial Property Rights Holder: 加藤健太郎、大川和久、升水紀郎
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2019-085952
  • Filing Date: 2019
• [Patent(Industrial Property Rights)] 抗トキソプラズマ剤及びこれを含む医薬 (2017)
  • Inventor(s): 加藤健太郎、アデエミ オルヨミ
  • Industrial Property Rights Holder: 国立大学法人帯広畜産大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-076436
  • Filing Date: 2017
• [Patent(Industrial Property Rights)] 抗原虫作用を持つ生薬由来化合物と生薬エキス (2017)
  • Inventor(s): 加藤 健太郎、村田 優穂、杉 達紀、野中 基弘
  • Industrial Property Rights Holder: 国立大学法人帯広畜産大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-083202
  • Filing Date: 2017
• [Patent(Industrial Property Rights)] 抗トキソプラズマ原虫剤 (2017)
  • Inventor(s): 加藤健太郎、アデエミ オルヨミ
  • Industrial Property Rights Holder: 国立大学法人帯広畜産大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-205211
  • Filing Date: 2017