• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanisms of qualitative and quantitative control of skeletal muscle tissue approached by hormones and glycosaminoglycan

Research Project

Project/Area Number 17H03934
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Integrative animal science
Research InstitutionTottori University

Principal Investigator

HOSAKA Yoshinao  鳥取大学, 農学部, 教授 (00337023)

Co-Investigator(Kenkyū-buntansha) 割田 克彦  鳥取大学, 農学部, 准教授 (40452669)
樋口 雅司  鳥取大学, 農学部, 講師 (70614791)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2019: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Keywordsホルモン / 糖鎖 / エストロゲン / コンドロイチン硫酸 / chst / ヒアルロニダーゼ / 筋芽細胞 / 再生 / 筋組織の損傷と再生 / OVXマウス / 骨格筋組織 / エストロゲン受容体 / 筋組織の再生 / 筋分化 / 脂肪分化 / 増殖と再生 / 脂肪細胞 / 線維芽細胞 / 発達と再生
Outline of Final Research Achievements

Based on histological analysis in the muscle repair process and analysis of chondroitin sulfate (CS) synthesis/degradation-related enzymes, CS is a factor that promotes the regulation of myoblast differentiation and the smooth transition to the differentiation stage of myoblasts. In addition, the tissue analysis and muscle mass recovery in the muscle repair process in the low estrogen (E2) state were analyzed using ovariectomized (OVX) mice and gene-modified mice. Tissue recovery is delayed when muscle damage is induced in a low E2 state, but it is recovered by additional administration of E2 to OVX mice. And it is suggested that E2 receptor Esr2 is involved in muscle regeneration more than Esr1.

Academic Significance and Societal Importance of the Research Achievements

ホルモンと糖鎖に主眼をおき、筋組織の修復過程でのこれら因子の関与の解明と筋量の制御を試みた。損傷筋組織の回復には、女性ホルモン・エストロゲン(E2)が重要であり、その際、E2の受容体であるEsr2を介する機構が主に機能していると考えられた。また、組織学的解析や検索対象とした糖鎖(コンドロイチン硫酸(CS))の合成や分解に関連する酵素の解析から、CSの合成/分解関連酵素は、炎症時に多量に発現し、それらは、炎症性細胞によって産生されている可能性を示した。CSは体内で筋分化制御因子として機能しているのかもしれない。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (14 results)

All 2020 2019 2018 2017 Other

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (10 results) (of which Int'l Joint Research: 4 results) Remarks (2 results)

  • [Journal Article] Neutralization of transforming growth factor (TGF)-β1 activity reduced fibrosis and enhanced regeneration of glycerol-injured rat muscle2020

    • Author(s)
      MAHDY Mohamed A. A.、WARITA Katsuhiko、HOSAKA Yoshinao Z.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 82 Issue: 2 Pages: 168-171

    • DOI

      10.1292/jvms.19-0446

    • NAID

      130007799362

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Compositional analysis of the glycosaminoglycan family in velvet antlers of Sika deer (Cervus nippon) at different growing stages.2019

    • Author(s)
      Takeda-Okuda N, Mizumoto S, Zhang Z, Kim SK, Lee CH, Jeon BT, Hosaka YZ, Kadomatsu K, Yamada S, Tamura JI.
    • Journal Title

      Glycoconj. J.

      Volume: 1 Issue: 2 Pages: 114-124

    • DOI

      10.1007/s10719-019-09859-1

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 筋再生過程で特徴的なコンドロイチン硫酸合成および同分解酵素の発現と産生細胞の同定2020

    • Author(s)
      保坂善真、鷲江壮太、割田克彦
    • Organizer
      第125回日本解剖学会総会・全国学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Comparative study of morphological change during myoregeneration in cardiotoxininjured muscle2020

    • Author(s)
      Chaiyasing Rattanatrai, Katsuhiko Warita, Yoshinao Z. Hosaka
    • Organizer
      第125回日本解剖学会総会・全国学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 筋再生過程でのコンドロイチン硫酸合成・分解酵素の動態2019

    • Author(s)
      鷲江壮太、割田克彦、保坂善真
    • Organizer
      第162回日本獣医学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Functional analysis of estrogen in the process of myogenesis and myoregeneration2019

    • Author(s)
      Rattanatrai Chaiyasing, Katsuhiko Warita, Yoshinao Z. Hosaka
    • Organizer
      The 7th Asian Association of Veterinary Anatomists
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of dynamics of chondroitin sulfate-synthesis and -degrading enzymes during muscle regeneration2019

    • Author(s)
      Sota Washie, Katsuhiko Warita, Yoshinao Z. Hosaka
    • Organizer
      The 7th Asian Association of Veterinary Anatomists
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Creation of scaffold derived from muscle tissue and evaluation of its function2019

    • Author(s)
      40)Yuhei Ogura, Sota Washie, Rattanatrai Chaiyasing, Katsuhiko Warita, Yoshinao Z. Hosaka;
    • Organizer
      The 7th Asian Association of Veterinary Anatomists
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 筋組織修復過程におけるコンドロイチン硫酸合成・分解関連酵素の発現とその機能2019

    • Author(s)
      保坂善真、鷲江壮太、ラッタナトライ=チャイヤシン、割田克彦
    • Organizer
      第38回日本糖質学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ニホンジカ(Cervus nippon)鹿茸と消化管に含まれるグリコサミノ グリカンの分析2019

    • Author(s)
      武田-奥田尚子、水本秀二、Zui Zhang、金守基、李致鎬、全炳台、延水晶、保坂善真、門松健治、山田修平、田村純一
    • Organizer
      第38回日本糖質学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] コンドロイチン硫酸を通して明らかとなった筋管の形成2018

    • Author(s)
      保坂善真
    • Organizer
      第161回日本獣医学会学術集会 獣医解剖分科会シンポジウム「透けてきたSkeleton構造-骨格筋・結合組織の形態形成機構とその利用-」
    • Related Report
      2018 Annual Research Report
  • [Presentation] IDENTIFICATION AND CHARACTERIZATION OF CHONDROITIN SULFATE SYNTHESIZING-CELLS IN DEER ANTLER2017

    • Author(s)
      Hosaka YZ, Suzuki A, Takeda-Okuda N, Tamura JI, Warita K
    • Organizer
      Eurocarb2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Remarks]

    • URL

      http://staff.muses.tottori-u.ac.jp/y-hosa/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 鳥取大学農学部ホームページ

    • URL

      http://staff.muses.tottori-u.ac.jp

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi