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Analysis of the cell functions depending on rare earth elements in the C1 microorganisms and its application to plant growth promoting system

Research Project

Project/Area Number 17H03949
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental agriculture(including landscape science)
Research InstitutionGifu University

Principal Investigator

Nakagawa Tomoyuki  岐阜大学, 応用生物科学部, 教授 (70318179)

Co-Investigator(Kenkyū-buntansha) 清水 将文  岐阜大学, 応用生物科学部, 准教授 (60378320)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2020: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2018: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Keywordsレアアース / メチロトローフ / 根粒菌 / 植物成長促進技術 / Methylobacterium属 / Bradyrhizobium属 / シロイヌナズナ / C1細菌 / 植物生育促進技術 / C1尾生物 / 植物生育促進 / C1微生物 / メチロバクテリウム属 / 多糖 / 植物共生細菌 / メチロバクテリウム属細菌
Outline of Final Research Achievements

In this research project, we aimed to elucidate the mechanism of activation of cell functions and plant growth promoting functions by REE of phytosymbiotic bacteria, such as methylotrophic bacteria and rhizobia. First, regarding the control mechanism of REE-dependent methanol metabolism of methylotrophic bacteria and rhizobia, the structural characteristics of methanol dehydrogenase (MDH) was shown, and regulation mechanism of its expression and activation mechanism of cell proliferation are mainly demonstrated. In addition, it could be to show the details of the structure of MDH from rhizobia and the uptake mechanism of REE into the cell.
In addition, we found the growth-promoting function of Arabidopsis thaliana by REE-dependent methylotrophic bacteria, and were able to show that it has the ability to promote root growth in particular.

Academic Significance and Societal Importance of the Research Achievements

本研究は、私たちが見出したREEを生命活動に利用する最初の事例であるREE依存型メタノール代謝系の生態系における機能と役割を解明するのみならず、その細胞機能を作物生育促進に活用するものである。REE依存型メタノール生育をする微生物は自然界に広く分布することが推測されており、その生態系での役割を知る以上にその細胞機能の新たな活用は大きな学術的・社会的意義を持つものと考えられる。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (10 results)

All 2021 2020 2019 2018 2017

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 6 results) Presentation (3 results)

  • [Journal Article] Discovery of lanthanide-dependent methylotrophy and screening methods for lanthanide-dependent methylotrophs2021

    • Author(s)
      Tani Akio、Mitsui Ryoji、Nakagawa Tomoyuki
    • Journal Title

      Methods in Enzymology

      Volume: 650 Pages: 1-18

    • DOI

      10.1016/bs.mie.2021.01.031

    • ISBN
      9780128238561
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of lanthanide-dependent methanol oxidation pathway in the legume symbiotic nitrogen-fixing bacterium Bradyrhizobium sp. strain Ce-32020

    • Author(s)
      Viagian Pastawan, Soya Suganuma, Kosuke Mizuno, Lun Wang, Akio Tani, Ryoji Mitsui, Kohei Nakamura, Masaya Shimada, Takashi Hayakawa, Nanung Agus Fitriyanto, and Tomoyuki Nakagawa
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 130 Issue: 6 Pages: 582-587

    • DOI

      10.1016/j.jbiosc.2020.07.012

    • NAID

      40022443010

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Preference for particular lanthanide species and thermal stability of XoxFs in Methylorubrum extorquens strain AM1.2020

    • Author(s)
      Wang L., Hibino A., Suganuma S., Ebihara A., Iwanoto S., Mitsiu R., Tani A. ,Shimada M., Hayakawa T., Nakagawa T.
    • Journal Title

      Enzyme and Microbial Technology

      Volume: 136 Pages: 109518-109518

    • DOI

      10.1016/j.enzmictec.2020.109518

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biological Function of Lanthanide in Plant-Symbiotic Bacteria: Lanthanide-Dependent Methanol Oxidation System2020

    • Author(s)
      Pastawan Viagian、Agus Fitriyanto Nanung、Nakagawa Tomoyuki
    • Journal Title

      Reviews in Agricultural Science

      Volume: 8 Issue: 0 Pages: 186-198

    • DOI

      10.7831/ras.8.0_186

    • NAID

      130007910010

    • ISSN
      2187-090X
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lanthanide-Dependent Methanol and Formaldehyde Oxidation in Methylobacterium aquaticum Strain 22A2020

    • Author(s)
      Patcha Yanpirat, Yukari Nakatsuji, Shota Hiraga, Yoshiko Fujitani, Terumi Izumi, Sachiko Masuda, Ryoji Mitsui, Tomoyuki Nakagawa and Akio Tani
    • Journal Title

      microorganisms

      Volume: 8 Issue: 6 Pages: 822-822

    • DOI

      10.3390/microorganisms8060822

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lanthanide-dependent methanol dehydrogenase from the legume symbiotic nitrogen-fixing bacterium Bradyrhizobium diazoefficiens strain USDA1102019

    • Author(s)
      Wang Lun、Suganuma Soya、Hibino Ayumi、Mitsui Ryoji、Tani Akio、Matsumoto Takashi、Ebihara Akio、Fitriyanto Nanung Agus、Pertiwiningrum Ambar、Shimada Masaya、Hayakawa Takashi、Nakagawa Tomoyuki
    • Journal Title

      Enzyme and Microbial Technology

      Volume: 130 Pages: 109371-109371

    • DOI

      10.1016/j.enzmictec.2019.109371

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lanthanide-Dependent Regulation of Methylotrophy in Methylobacterium aquaticum Strain 22A2018

    • Author(s)
      Masuda Sachiko、Suzuki Yutaka、Fujitani Yoshiko、Mitsui Ryoji、Nakagawa Tomoyuki、Shintani Masaki、Tani Akio
    • Journal Title

      mSphere

      Volume: 3 Issue: 1

    • DOI

      10.1128/msphere.00462-17

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] ランタノイドSmはMethylorubrum属細菌の低栄養環境下での生育を促進させる.2019

    • Author(s)
      水野洸介, 原田雄斗, 岩本悟志, 島田昌也, 早川享志, 中川智行.
    • Organizer
      第71回 日本生物工学会 大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ホウレンソウのポストハーベストにおける菌叢変遷と鮮度劣化へのPPFMsの関与.2019

    • Author(s)
      Wang Lun, 繁原安美, 阪口由佳, 谷明生,中野浩平, 島田昌也, 早川享志, 中川智行.
    • Organizer
      第71回 日本生物工学会 大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 植物共生細菌によるレアアース元素の利用 ―新奇なレアアース依存型メタノール代謝系―2017

    • Author(s)
      中川智行
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2022-01-27  

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