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Development of mycovirus resources and its usage

Research Project

Project/Area Number 17H03950
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental agriculture(including landscape science)
Research InstitutionNagoya University

Principal Investigator

CHIBA Sotaro  名古屋大学, 生命農学研究科, 准教授 (70754521)

Co-Investigator(Kenkyū-buntansha) 藤森 文啓  東京家政大学, 家政学部, 教授 (50318226)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2020: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2019: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2018: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Keywordsマイコウイルス / 代謝攪乱 / IRES / 発現ベクター / 菌類ウイルス / RNAウイルス / 翻訳機構 / 代謝産物 / 病原性制御 / マイコトキシン / 翻訳開始機構 / 多重遺伝子発現 / 代謝変化 / 宿主抵抗性 / 有効利用 / 毒素 / 遺伝資源
Outline of Final Research Achievements

Mycoviruses are discovered with the potential to regulate non-welcomed fungi such as phytopathogenic fungi. It leads an intensive virus hunting worldwide. In this study, 40 mycoviruses are newly identified and characterized, that are mostly isolated from Fusarium species. The study found some of those viruses altered basic metabolites productivity and mycotoxin biogenesis, suggesting the viruses are fundamental disturbant element in fungi In addition, the study identified virus cis-acting elements important to virus gene expressions, and applied these for multiple expression of foreign gene in filamentous fungi, for the first time. Overall, the study showed new aspects of mycoviruses that are useful as an experimental tool and a fungal metabolism disturber.

Academic Significance and Societal Importance of the Research Achievements

本研究では,菌類ウイルス(マイコウイルス)を有用資源と捉え,その利用価値について検証を行った。新規ウイルスの分離と多様性を明らかにし,予てから期待されている植物病原菌制御に加え,カビ毒生産制御,代謝攪乱,ウイルス遺伝子発現機構を利用した新規発現ベクターの開発において利用価値を見出した.また,ウイルス感染機構,ウイルス-宿主間のせめぎ合いやゲノム決定法の評価も行い,学術的に重要な知見を多数得ることができた。これらの知見は,菌類ウイルスが有用資源であることを端的に示しており,病害防除,代謝変動への外的刺激因子,菌類における実験ツール提供という点で社会への還元が期待される。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (30 results)

All 2021 2020 2019 2018 2017 Other

All Int'l Joint Research (9 results) Journal Article (6 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 6 results,  Open Access: 6 results) Presentation (13 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results) Remarks (2 results)

  • [Int'l Joint Research] University of Bologna(イタリア)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] National Agricultural Research Centre(パキスタン)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Addis Ababa S & T University(エチオピア)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Bologna(イタリア)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] National Agricultural Research Centre(パキスタン)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Addis Ababa S & T University(エチオピア)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Bologna(イタリア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] National Agricultural Research Centre(パキスタン)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Addis Ababa S & T University(エチオピア)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Cryphonectria nitschkei chrysovirus 1 with unique molecular features and a very narrow host range.2021

    • Author(s)
      Shahi, S., Chiba, S., Kondo, H., and Suzuki, N.
    • Journal Title

      Virology

      Volume: 554 Pages: 55-65

    • DOI

      10.1016/j.virol.2020.11.011

    • NAID

      120007174661

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] De novo sequencing of novel mycoviruses from Fusarium sambucinum: An attempt on direct RNA sequencing of viral dsRNAs2021

    • Author(s)
      Mizutani Y, Uesaka K, Ota A, Calassanzio M, Ratti C, Suzuki T, Fujimori F, Chiba S.
    • Journal Title

      Frontiers in Microbiology

      Volume: 12

    • DOI

      10.3389/fmicb.2021.641484

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Two novel fungal negative-strand RNA viruses related to mymonaviruses and phenuiviruses in the shiitake mushroom (Lentinula edodes).2019

    • Author(s)
      Lin YH, Fujita M, Chiba S, Hyodo K, Andika IB, Suzuki N, Kondo H
    • Journal Title

      Virology

      Volume: 533 Pages: 125-136

    • DOI

      10.1016/j.virol.2019.05.008

    • NAID

      120006847074

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Novel mitoviruses and a unique tymo-like virus in hypovirulent and virulent strains of the Fusarium Head Blight fungus, Fusarium boothii2018

    • Author(s)
      Mizutani, Y., Abraham, A., Uesaka, K., Kondo, H., Suga, H., Suzuki, N., Chiba, S.
    • Journal Title

      Viruses

      Volume: 10 Issue: 11 Pages: 584-584

    • DOI

      10.3390/v10110584

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] ICTV Virus Taxonomy Profile: Quadriviridae2018

    • Author(s)
      Chiba Sotaro、Caston Jose R.、Ghabrial Said A.、Suzuki Nobuhiro、ICTV Report Consortium
    • Journal Title

      Journal of General Virology

      Volume: 99 Issue: 11 Pages: 1480-1481

    • DOI

      10.1099/jgv.0.001152

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] First evidence for internal ribosomal entry sites in diverse fungal virus genomes.2018

    • Author(s)
      Chiba, S., Jamal, A., and Suzuki. N.
    • Journal Title

      mBio

      Volume: 9 Issue: 2

    • DOI

      10.1128/mbio.02350-17

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 菌類ウイルス新規IRESエレメントの探索および多重遺伝子発現系への応用2020

    • Author(s)
      村田佳乃子・Matteo Calassanzio・鈴木信弘・千葉壮太郎
    • Organizer
      令和3年度植物病理学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 植物共生菌Epichloe festucaeの抗ウイルス防御機構2020

    • Author(s)
      佐藤優貴子・生川千晶・佐藤育男・竹本大吾・鈴木信弘・千葉壮太郎
    • Organizer
      令和2年度日本植物病理学会関西部会
    • Related Report
      2020 Annual Research Report
  • [Presentation] タイ産イネ褐色菌核病菌に感染する新規アルファパルティティウイルスの性状解析2020

    • Author(s)
      ニアン ソックティ・シュバ ダス・近藤秀樹・鈴木信弘・荒川征夫・ラッタナクリータク チャイナロン・ポンピスッタ ラティア・千葉壮太郎
    • Organizer
      令和2年度日本植物病理学会関西部会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Mycoviruses infecting Fusarium species isolated from Ethiopian wheat fields and their potential association with hypovirulece of a Fusarium Head Bright fungus.2019

    • Author(s)
      Mizutani Y., Adane A., Uesaka K., Kondo H., Suga H., Suzuki N. and Chiba S.
    • Organizer
      XVIII International Congress on Molecular Plant-Microbe Interactions (Glasgow, Scotland)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of internal ribosomal entry sites in the RNA genome of a hypovirulence-conferring mycovirus of the white root rot fungus.2019

    • Author(s)
      Murata K., Jamal A., Kubo H., Chiba S., and Suzuki N.
    • Organizer
      Asian Mycological Congress 2019 (Tsu)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Potential contribution of a defective RNA segment of Fusarium boothii large flexivirus 1 on hypovirulence of the host Fusarium Head Blight fungus.2019

    • Author(s)
      Chiba S., Mizutani Y., Adane A., Uesaka K., Kondo H., Suga H. and Suzuki N.
    • Organizer
      Asian Mycological Congress 2019 (Tsu)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 植物病原糸状菌を制御する菌類ウイルスの探索とゲノム解析の迅速化2019

    • Author(s)
      水谷行善, カラッサンチオ マッテオ, 太田綺弥, 藤森文啓, 上坂 一馬, 千葉壮太郎.
    • Organizer
      物工学会大会(岡山)
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 糸状菌ウイルスゲノム中の新規IRES探索とゲノム比較2019

    • Author(s)
      村田佳乃子, Calassanzio Matteo, 久保弘法, Jamal Atif, 鈴木信弘, 千葉壮太郎
    • Organizer
      令和2年度 日本植物病理学会(開催中止・発表扱い)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 本邦産 Fusarium 属菌に感染するマイコウイルスの探索および生物学的解析2018

    • Author(s)
      太田綺弥・水谷行善・佐藤育男・竹本大吾・川北一人・藤森文啓・千葉壮太郎
    • Organizer
      平成31年度 日本植物病理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Fusarium boothii 病原性衰退株BL13に感染する新規Tymovirales目ウイルスの性状解析2018

    • Author(s)
      水谷行善・Adane Abraham・上坂一馬・近藤秀樹・須賀晴久・鈴木信弘・千葉壮太郎
    • Organizer
      平成31年度 日本植物病理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] メガビルナウイルスRnMBV1のIRES領域の同定2018

    • Author(s)
      村田佳乃子・Atif Jamal・久保弘法・鈴木信弘・千葉壮太郎
    • Organizer
      平成31年度 日本植物病理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Host-specific RNA silencing (Dicer)-mediated virus interference revealed by characterizing a new fungal partitivirus2017

    • Author(s)
      Sotaro Chiba, Annisa Aulia, Sakae Hisano, Lin YuHsin, Hideki Kondo, Satoko Kanematsu, Hajime Yaegashi, and Nobuhiro Suzuki
    • Organizer
      4th International Mycovirus Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characterization of mitoviruses found in Ethiopian isolates of Fusarium spp.2017

    • Author(s)
      Yukiyoshi Mizutani, Adane M Abraham, Haruhisa Suga, Nobuhiro Suzuki, Sotaro Chiba
    • Organizer
      4th International Mycovirus Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 原著論文

    • URL

      https://nagoyaplantpathol.wixsite.com/nagoya-plant-pathol/blank-8

    • Related Report
      2019 Annual Research Report
  • [Remarks] 植物病理学研究室 原著論文

    • URL

      https://nagoyaplantpathol.wixsite.com/nagoya-plant-pathol/blank-8

    • Related Report
      2018 Annual Research Report

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Published: 2017-04-28   Modified: 2022-01-27  

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