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Control of the penetration of toxic heavy metal elements into plant roots - Establishment of a research base for vegetation restoration

Research Project

Project/Area Number 17H03952
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental agriculture(including landscape science)
Research InstitutionTokyo University of Agriculture

Principal Investigator

Nakamura Shin-ichi  東京農業大学, 生命科学部, 教授 (00322339)

Co-Investigator(Kenkyū-buntansha) 中井 雄治  弘前大学, 地域戦略研究所, 教授 (10321788)
鈴井 伸郎  国立研究開発法人量子科学技術研究開発機構, 高崎量子応用研究所 放射線生物応用研究部, 主幹研究員 (20391287)
頼 泰樹  秋田県立大学, 生物資源科学部, 准教授 (30503099)
野下 浩二  秋田県立大学, 生物資源科学部, 准教授 (40423008)
大津 直子  東京農工大学, (連合)農学研究科(研究院), 教授 (40513437)
及川 彰  山形大学, 農学部, 教授 (50442934)
尹 永根  国立研究開発法人量子科学技術研究開発機構, 高崎量子応用研究所 放射線生物応用研究部, 主任研究員 (50609708)
俵谷 圭太郎  山形大学, 農学部, 教授 (70179919)
野副 朋子  明治学院大学, 教養教育センター, 准教授 (90590208)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Keywordsグルタチオン / カドミウム / 亜鉛 / ポジトロンイメージング / 銅 / PETIS
Outline of Final Research Achievements

Establishment of cultivation technology for field crops with low accumulation of cadmium (Cd), one of the threats to food safety, is an urgent issue. Our previous research has shown that glutathione, given to plant roots site-specifically, selectively inhibits Cd translocation from roots to shoots and Cd accumulation in shoots of oilseed rape plants. Molecular mechanisms of this phenomenon are not fully understood. This study revealed that glutathione, applied to roots, activate Cd return to roots at the shoot base. Our research also demonstrated glutathione has different functions depending on the site where it is applied. Glutathione applied to leaves activated Zinc (Zn) translocation and Zn accumulation in shoots.

Academic Significance and Societal Importance of the Research Achievements

グルタチオンによって活性化されるカドミウム動態を制御する複数の物質動態制御機構の存在を明らかにできた。様々な生理活性を持つグルタチオンに新たな生理学的な意義を見出すことができた。
本研究で得られた成果を応用展開することで、低Cd 吸収作物を持続的に生産するための新たな栽培技術を創出できる。そして、この新規栽培技術は、多くの生産現場が望む遺伝子組み換え技術を利用しない、即効性のある畑作物のCd 対策となり得る。さらに有害重金属元素の植物根への侵入を抑制し、効果的に根細胞壁に留めることができれば、植物の重金属耐性を強化でき、重金属元素汚染土壌での植物(農作物)の栽培も可能になる。

Report

(5 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (11 results)

All 2021 2019 2018 Other

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (6 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] Shoot base responds to root-applied glutathione and functions as a critical region to inhibit cadmium translocation from the roots to shoots in oilseed rape (Brassica napus)2021

    • Author(s)
      Li, J.-S., Suzui, N., Nakai, Y., Yin, Y.-G., Ishii, S., Fujimaki, S., Kawachi, N., Rai, H., Matsumoto, T., Sato-Izawa, K., Ohkama-Ohtsu, N., and Nakamura S.
    • Journal Title

      Plant Science

      Volume: 305 Pages: 110822-110822

    • DOI

      10.1016/j.plantsci.2021.110822

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Phloem-specific overexpression of AtOPT6 in Arabidopsis enhances Zn transport into shoots2019

    • Author(s)
      Wongkaew Arunee、Nakamura Shin-ichi、Sekimoto Hitoshi、Yokoyama Tadashi、Ohkama-Ohtsu Naoko
    • Journal Title

      Plant Science

      Volume: in Press Pages: 91-98

    • DOI

      10.1016/j.plantsci.2019.04.022

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Foliar-applied glutathione activates zinc transport from roots to shoots in oilseed rape2018

    • Author(s)
      Nakamura Shin-ichi、Wongkaew Arunee、Nakai Yuji、Rai Hiroki、Ohkama-Ohtsu Naoko
    • Journal Title

      Plant Science

      Volume: - Pages: 424-434

    • DOI

      10.1016/j.plantsci.2018.10.018

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Elevated glutathione synthesis in leaves contributes to zinc transport from roots to shoots in Arabidopsis2018

    • Author(s)
      Wongkaew Arunee、Nakamura Shin-ichi、Suzui Nobuo、Yin Yong-Gen、Ishii Satomi、Kawachi Naoki、Kojima Katsuhiro、Sekimoto Hitoshi、Yokoyama Tadashi、Ohkama-Ohtsu Naoko
    • Journal Title

      Plant Science

      Volume: - Pages: 416-423

    • DOI

      10.1016/j.plantsci.2018.11.003

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] ポジトロンイメージング技術を利用して高等植物のカドミウム動態制御機構を明らかにする2021

    • Author(s)
      中村進一
    • Organizer
      第58回アイソトープ・放射線研究会(招待講演)
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] グルタチオンが植物の銅耐性機構に及ぼす影響2021

    • Author(s)
      中村進一
    • Organizer
      短寿命RI供給プラットフォーム成果報告会
    • Related Report
      2020 Annual Research Report
  • [Presentation] アブラナの葉へ施用したグルタチオンは根から地上部への亜鉛の移行を活性化する2019

    • Author(s)
      中村進一、ワオンケオアルニー、中井雄治、頼泰樹、大津直子
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Assessment of glutathione transporter AtOPT6 functions on sink organs in Arabidopsis2018

    • Author(s)
      Arunee Wongkaew, Shin-ichi Nakamura, Tadashi Yokoyama, Naoko Ohkama-Ohtsu
    • Organizer
      日本土壌肥料学会2018年度神奈川大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] カドミウム存在下での根へのグルタチオン添加に応答する遺伝子の発現解析および導管液と篩管液のメタボロミクス解析2018

    • Author(s)
      李俊松、及川彰、中井雄治、中村進一、大津直子
    • Organizer
      日本土壌肥料学会2018年度神奈川大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Evaluation of the effect of glutathione in root zone on cadmium behaviors in oilseed rape plants2018

    • Author(s)
      S. Nakamura, N. Suzui, Y.-G. Yin, S. Ishii, S. Fujimaki, N. Kawachi, K. Noge, H. Rai, K. Izawa-Sato, T. Matsumoto
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Remarks] リサーチマップ

    • URL

      https://researchmap.jp/read0072042

    • Related Report
      2020 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2023-01-30  

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