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Evolution process of microbes in subseafloor

Research Project

Project/Area Number 17H03956
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental agriculture(including landscape science)
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

HOSHINO Tatsuhiko  国立研究開発法人海洋研究開発機構, 超先鋭研究開発部門(高知コア研究所), 主任研究員 (30386619)

Co-Investigator(Kenkyū-buntansha) 浦本 豪一郎  高知大学, 海洋コア総合研究センター, 特任助教 (70612901)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2019: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Keywords海底下堆積物 / 微生物 / 微生物多様性 / 海底下微生物 / 進化 / 微生物生態 / 掘削化学 / 海底下生命圏 / IODP / 生物多様性 / メタゲノム解析 / ゲノム / 海底下 / 次世代シーケンサー / 堆積物 / 海洋堆積物
Outline of Final Research Achievements

Below the seafloor, which accounts for 70% of the Earth's surface, there is a unique ecosystem that is different from the surface biosphere, including ocean and soil, and a huge number of microorganisms have been confirmed to exist. In this study, we investigated how the ecosystem was constructed in the low energy subseafloor environment by analyzing sediments from 0-600 m below the seafloor. Microbial community structure in subseafloor differed significantly between anaerobic-eutrophic and aerobic-oligotrophic environments, with the archaea/bacteria ratio in the former being significantly higher than in the latter. The proportion of archaeal cells in the total sediment was estimated to be about 37%, comparable to that in the ocean.

Academic Significance and Societal Importance of the Research Achievements

統一された高精度なDNA分析により、全地球レベルで海底下堆積物中の微生物群集の比較解析を世界で初めて行なった。我々の研究成果は、海底下の生態系がどのように構築されてきたのか、どの程度多様なのかという基礎的ではあるがこれまでに分かっていなかった問題の答えを与えるものである。また、本研究成果は、地上のエネルギーに満ちた環境とは全く異なる海底下という極限環境でどのように生命が進化・適応してきたのか明らかにするための進化学的研究の礎となるものである。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (26 results)

All 2019 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (10 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 10 results,  Open Access: 9 results) Presentation (14 results) (of which Int'l Joint Research: 9 results,  Invited: 1 results)

  • [Int'l Joint Research] University of Rhode Island(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Bremen(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Draft Genome Sequences of Penicillium spp. from Deeply Buried Oligotrophic Marine Sediments2019

    • Author(s)
      Sobol Morgan S.、Hoshino Tatsuhiko、Futagami Taiki、Inagaki Fumio、Kiel Reese Brandi
    • Journal Title

      Microbiology Resource Announcements

      Volume: 8 Issue: 6

    • DOI

      10.1128/mra.01613-18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cultivable microbial community in 2-km-deep, 20-million-year-old subseafloor coalbeds through ~1000 days anaerobic bioreactor cultivation2019

    • Author(s)
      Imachi Hiroyuki、Tasumi Eiji、Takaki Yoshihiro、Hoshino Tatsuhiko、Schubotz Florence、Gan Shuchai、Tu Tzu-Hsuan、Saito Yumi、Yamanaka Yuko、Ijiri Akira、Matsui Yohei、Miyazaki Masayuki、Morono Yuki、Takai Ken、Hinrichs Kai-Uwe、Inagaki Fumio
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1

    • DOI

      10.1038/s41598-019-38754-w

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Microbial dormancy in the marine subsurface: Global endospore abundance and response to burial2019

    • Author(s)
      Woermer Lars、Hoshino Tatsuhiko、Bowles Marshall W.、Viehweger Bernhard、Adhikari Rishi R.、Xiao Nan、Uramoto Go-ichiro、K?nneke Martin、Lazar Cassandre S.、Morono Yuki、Inagaki Fumio、Hinrichs Kai-Uwe
    • Journal Title

      Science Advances

      Volume: 5 Issue: 2

    • DOI

      10.1126/sciadv.aav1024

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Microbial Metabolism and Community Dynamics in Hydraulic Fracturing Fluids Recovered From Deep Hydrocarbon-Rich Shale2019

    • Author(s)
      Morono Yuki、Wishart Jessie R.、Ito Motoo、Ijiri Akira、Hoshino Tatsuhiko、Torres Marta、Verba Circe、Terada Takeshi、Inagaki Fumio、Colwell Frederick S.
    • Journal Title

      Frontiers in Microbiology

      Volume: 10 Pages: 1-14

    • DOI

      10.3389/fmicb.2019.00376

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Deep-biosphere methane production stimulated by geofluids in the Nankai accretionary complex2018

    • Author(s)
      Ijiri Akira et al.
    • Journal Title

      Science Advances

      Volume: 4 Issue: 6 Pages: 4631-4631

    • DOI

      10.1126/sciadv.aao4631

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments2018

    • Author(s)
      Morono Yuki、Hoshino Tatsuhiko、Terada Takeshi、Suzuki Taketo、Sato Takahiro、Yuasa Hisashi、Kubota Yuji、Inagaki Fumio
    • Journal Title

      Microbes and Environments

      Volume: 33 Issue: 2 Pages: 222-226

    • DOI

      10.1264/jsme2.ME18012

    • NAID

      130007393763

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Abundance and distribution of Archaea in the subseafloor sedimentary biosphere2018

    • Author(s)
      Hoshino Tatsuhiko、Inagaki Fumio
    • Journal Title

      The ISME Journal

      Volume: 13 Issue: 1 Pages: 227-231

    • DOI

      10.1038/s41396-018-0253-3

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] In-situ mechanical weakness of subducting sediments beneath a plate boundary d?collement in the Nankai Trough2018

    • Author(s)
      Yohei Hamada, Takehiro Hirose, Akira Ijiri, Yasuhiro Yamada, Yoshinori Sanada, Saneatsu Saito, Noriaki Sakurai, Takamitsu Sugihara, Takahiro Yokoyama, Tomokazu Saruhashi, Tatsuhiko Hoshino, Nana Kamiya, Stephen Bowden, Margaret Cramm, Susann Henkel, Kira Homola, Hiroyuki Imachi, Masanori Kaneko, (他23名)
    • Journal Title

      Progress in Earth and Planetary Science

      Volume: 5 Issue: 1 Pages: 1-70

    • DOI

      10.1186/s40645-018-0228-z

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Methyl-compound use and slow growth characterize microbial life in 2-km-deep subseafloor coal and shale beds2017

    • Author(s)
      Trembath-Reichert, E., Morono, Y., Ijiri, A., Hoshino, T., Dowson, K. S., Inagaki, F., and Orphan, V. J.
    • Journal Title

      Proc. Natl. Acad. Sci. U.S.A.

      Volume: 114 Issue: 44

    • DOI

      10.1073/pnas.1707525114

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Atribacteria from the Subseafloor Sedimentary Biosphere Disperse to the Hydrosphere through Submarine Mud Volcanoes2017

    • Author(s)
      Hoshino, T., Toki, T., Ijiri, A., Morono, Y., Machiyama, H., Ashi, J., Okamura, K., and Inagaki, F.
    • Journal Title

      Frontiers in Microbiology

      Volume: 8 Pages: 1135-1135

    • DOI

      10.3389/fmicb.2017.01135

    • NAID

      120006383302

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Interaction between submarine landslides and subseafloor sedimentary microbiome: Chikyu SCORE Expedition 910 off Cape Erimo2019

    • Author(s)
      星野辰彦、浦本豪一郎、藤内智士、諸野祐樹、井尻暁、鈴木志野、久保雄介、稲垣史生
    • Organizer
      JpGU2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Global diversity of subseafloor sedimentary microbiome2019

    • Author(s)
      Tatsuhiko Hoshino, Hideyuki Doi, Go-Ichiro Uramoto, Lars Woemar, Rishi R. Adikari, Nan Xiao, Yuki Morono, Steven D'Hondt, Kai-Uwe Hinrichs, Fumio Inagaki
    • Organizer
      Deep Carbon 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 次世代シーケンシングを用いた核酸定量法の環境影響評価への適用2019

    • Author(s)
      猪又 健太郎, 北橋 倫, 西島 美由紀, 星野 辰彦, 下田代 雅文, 山本 啓之
    • Organizer
      ブルーアースサイエンス・テク2019
    • Related Report
      2018 Annual Research Report
  • [Presentation] Distribution of aerobic microbial activities in ultra-oligotrophic sediments of the South Pacific Gyre2018

    • Author(s)
      YUKI MORONO, TAKESHI TERADA, MOTOO ITO, STEVEN D'HONDT, TATSUHIKO HOSHINO, FUMIO INAGAKI
    • Organizer
      日本地球惑星科学連合大会
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 海底下深部生命圏から海水中への泥火山を通した微生物の拡散2018

    • Author(s)
      星野辰彦、土岐知弘、井尻 暁、諸野祐樹、芦寿一郎、稲垣史生
    • Organizer
      日本微生物生態学会第32回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] クリーンフィルターはDNAエアロゾルを止められるのか?汚染除去効果の検証2018

    • Author(s)
      諸野祐樹、星野辰彦、寺田武志、鈴木剛人、佐藤卓広、湯浅久史、久保田裕仁、稲垣史生
    • Organizer
      極限環境生物学会2018年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 海底下堆積物中に残存する真核生物由来DNAの分布:古代生態系復元への応用の可能性を探る2018

    • Author(s)
      星野 辰彦、肖 楠、浦本 豪一郎、諸野 祐樹、Rishi Adhikari、稲垣 史生
    • Organizer
      日本地球惑星科学連合大会
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 微小生命活動の現場を視る-X線マイクロ/ナノCTによる微生物細胞可視化の試み2018

    • Author(s)
      諸野祐樹、浦本豪一郎、上杉健太朗、竹内晃久、上椙真之、久保田健吾、星野辰彦、稲垣史生
    • Organizer
      微小生命活動の現場を視る-X線マイクロ/ナノCTによる微生物細胞可視化の試み
    • Related Report
      2018 Annual Research Report
  • [Presentation] 海底下深部堆積物中の真核生物環境DNAによる古代生態系の復元2018

    • Author(s)
      星野辰彦、伊達康博、肖楠、浦本豪一郎、諸野祐樹、稲垣史生
    • Organizer
      第1回 環境DNA学会 東京大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Global diversity of subseafloor microbial community2018

    • Author(s)
      Hoshino Tatsuhiko, Doi Hideyuki, Wormer Lars, Morono Yuki, D'Hondt Steven, Hinrichs Kai-Uwe, Inagaki Fumio
    • Organizer
      AGU fall meeting 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Exploration of the sedimentary biosphere in Bengal Fan (IODP Exp. 354)2018

    • Author(s)
      Adhikari Rishi, Heuer Verena, Elvert Marcus, Hoshino Tatsuhiko, Inagaki Fumio, Kallmeyer Jens, Morono Yuki, Wormer Lars, Hinrichs Kai-Uwe
    • Organizer
      AGU fall meeting 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The global survey of subseafloor sedimentary microbiomes2017

    • Author(s)
      TATSUHIKO HOSHINO, HIDEYUKI DOI, LARS WORMER, YUKI MORONO, STEVEN D’HONDT, KAI-UWE HINRICHS, FUMIO INAGAKI
    • Organizer
      Goldschmidt 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Global microbial diversity in subseafloor sediment2017

    • Author(s)
      Tatsuhiko Hoshino, Hideyuki Doi, Lars Wormer, Yuki Morono, Kai-Uwe Hinrichs, Fumio Inagaki
    • Organizer
      The international society for subsurface microbiology 2017 conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The deep biosphere in Bengal Fan sediments (IODP Exp. 354)2017

    • Author(s)
      R.R. ADHIKARI, V.B. HEUER, M. ELVERT, T. HOSHINO, F. INAGAKI, J. KALLMEYER, A. KITTE, L. WOERMER, K.-U. HINRICHS
    • Organizer
      Goldschmidt2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2021-02-19  

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