| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2019: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
Pseudomonas chlororaphis B23 yields nitrile hydratase (NHase) used for the production of 5- cyanovaleramide at the industrial level.
Here, we constructed several plasmids for the expression of NHases (and these mutant enzymes) in the presence and absence of each accessory protein of the NHase. Besides the expression of each of these NHases in E. coli carrying the resultant plasmid, we purified and characterized them. Based on the results of a series of experiments, we clarified the mechanisms for post-translational activation of NHase.
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