Analyses of plant parasitic nematode infection mechanisms

• Project/Area Number: 17H03967
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Kumamoto University
• Principal Investigator: Sawa Shinichiro 熊本大学, 大学院先端科学研究部(理), 教授 (00315748)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
• Keywords: 植物感染性線虫 / 植物感染性センチュウ / サツマイモネコブセンチュウ

Outline of Final Research Achievements

Seed or root exudate components are known to regulate nematode behaviours, however currently it’s unclear how plant affect the spermosphere or rhizosphere microbiota composition. Here we showed compounds associated with the seed coat mucilage or root exudates can regulate the behaviour of soil-borne pathogens, the root-knot nematodes, M. incognita.
We found that flax seed exudate is capable of attracting RKN in a dose-dependent manner. The RKN attractant could be purified from flax mucilage and consists of a hydrophilic carbohydrate derivatives. On the other hand, we screened a chemical library of synthetic compound for M. incognita attractants. M. incognita were found to be attracted specifically by natural compounds that possess three to five methylene groups between two terminal amino groups. Using cryo-TOF-SIMS/SEM, one of the diamine was indeed detected in soybean root cortex cells and the surrounding rhizosphere, establishing a chemical gradient.

Academic Significance and Societal Importance of the Research Achievements

本研究により、線虫の植物誘引物質が同定出来た。また、植物への感染過程における分子機構の一端も明らかになった。これらの情報は、学術的には、植物－動物相互作用の分子機構が明らかになっただけでなく、どのようにパラサイトーホストの関係が進化してきたかを紐解く鍵になると考えられる。また、これらの情報は、線虫のトラップ剤の開発や、線虫に抵抗性を持つ作物品種の開発にも繋がると考えられ、基礎研究だけでなく、応用研究分野にも、今後、広く、利用されると考えられる。

Research Products

• [Int'l Joint Research] INRA/ENS Lyon(フランス)
• [Int'l Joint Research] UNIVERSIDAD DE CASTILLA LA MANCHA(スペイン)
• [Int'l Joint Research] INRA/ENS Lyon(フランス)
• [Int'l Joint Research] UNIVERSIDAD DE CASTILLA LA MANCHA(スペイン)
• [Journal Article] Effect of the CLE14 polypeptide on <i>GLABRA2</i> homolog gene expression in rice and tomato roots (2019)
  • Author(s): Hayashi, N., Rongkavilit, N., Tetsumura, T., Sawa, S., Wada, T., and Tominaga-Wada, R.
  • Journal Title: Plant Biotechnology Volume: 36 Issue: 3 Pages: 205-208
  • DOI: 10.5511/plantbiotechnology.19.0725a
  • NAID: 130007724770
  • ISSN: 1342-4580, 1347-6114
  • Year and Date: 2019-09-25
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A new forma of the mycoheterotrophic orchid Lechanorchis japonica from Japan (2019)
  • Author(s): Suetsugu K., Kaida, S., Fukunaga, H., and Sawa, S.
  • Journal Title: Acta Phytotax. Geobot. Volume: In Press
  • Peer Reviewed
• [Journal Article] The RopGEF KARAPPO Is Essential for the Initiation of Vegetative Reproduction in Marchantia polymorpha (2019)
  • Author(s): Hiwatashi T., Goh H., Yasui Y., Koh L.Q., Takami H., Kajikawa M., Kirita H., Kanazawa T., Minamino N., Togawa T., Sato M., Wakazaki M., Yamaguchi K., Shigenobu S., Fukaki H., Mimura T., Toyooka K., Sawa S., Yamato K.T.,Ueda T., Urano D., Kohchi T., Ishizaki K.
  • Journal Title: Current Biology Volume: 29 Issue: 20 Pages: 3525-3531
  • DOI: 10.1016/j.cub.2019.08.071
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Developing heritable mutations in Arabidopsis thaliana using a modified CRISPR/Cas9 toolkit comprising PAM-altered Cas9 variants and gRNAs. (2019)
  • Author(s): Yamamoto, A., Ishida, T., Yoshimura, M., Kimura, Y., Sawa, S.
  • Journal Title: Plant Cell Physiol Volume: 60 Issue: 10 Pages: 2255-2262
  • DOI: 10.1093/pcp/pcz118
  • Peer Reviewed
• [Journal Article] Lecanorchis moritae (Orchidaceae, Vanilloideae), a new mycoheterotrophic species from Amami- Oshima Island, Japan, based on morphological and molecular data (2019)
  • Author(s): Suetsugu, K., Kaida, S., Hsu, T.-C., Sawa, S.
  • Journal Title: Phytotaxa Volume: 404 Pages: 137-145
  • DOI: 10.5962/bhl.title.154048
  • NAID: 120006951628
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Diverse function of plant peptide hormones in local signaling and development (2019)
  • Author(s): Hirakawa Yuki、Sawa Shinichiro
  • Journal Title: Current Opinion in Plant Biology Volume: 51 Pages: 81-87
  • DOI: 10.1016/j.pbi.2019.04.005
  • Peer Reviewed
• [Journal Article] Control of proliferation in the haploid meristem by CLE peptide signaling in Marchantia polymorpha. (2019)
  • Author(s): Hirakawa Y, Uchida N, Yamaguchi YL, Tabata R, Ishida S, Ishizaki K, Nishihama R, Kohchi T, Sawa S, Bowman JL
  • Journal Title: PLoS Genet. Volume: 7 Issue: 3 Pages: e1007997-e1007997
  • DOI: 10.1371/journal.pgen.1007997
  • NAID: 120006621164
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Regulation of Root-Knot Nematode Behavior by Seed-Coat Mucilage-Derived Attractants. (2019)
  • Author(s): Tsai, A, Y-L., Higaki, T., Nguyen, C-N., Perfus-Barbeoch, L., Favery, B., and Sawa, S.
  • Journal Title: Mol. Plants. Volume: 12 Issue: 1 Pages: 99-122
  • DOI: 10.1016/j.molp.2018.11.008
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] CLE9 peptide-induced stomatal closure is mediated by abscisic acid, hydrogen peroxide, and nitric oxide in Arabidopsis thaliana 5.発行年 2019年 (2019)
  • Author(s): Zang, L., Shi, X., Zhang, Y., Wang, J., Yang, J., Ishida, T., Jiang, W., Han, X., Kang, J., Wang, X., Pan, L., Lv, S., Cao, B., Zhang, Y., Wu, J., Han, H., Hu, Z., Cui, L, Sawa, S., He, J., and Wang, G
  • Journal Title: Plant Cell & Environment. Volume: 42 Issue: 3 Pages: 1033-1044
  • DOI: 10.1111/pce.13475
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The CLE9/10 secretory peptide regulates stomatal and vascular development through distinct receptors. (2018)
  • Author(s): Qian P, Song W, Yokoo T, Minobe A, Wang G, Ishida T, Sawa S, Chai Jijie; Kakimoto T.
  • Journal Title: Nature Plants Volume: 4 Issue: 12 Pages: 1071-1081
  • DOI: 10.1038/s41477-018-0317-4
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 3D Body Evolution: Adding a New Dimension to Colonize the Land (2018)
  • Author(s): Furumizu, C., Hirakawa, Y., Bowman, J. L., and *Sawa, S.
  • Journal Title: Curr. Biol. Volume: 28 Issue: 15 Pages: 838-840
  • DOI: 10.1016/j.cub.2018.06.040
  • Peer Reviewed
• [Journal Article] The dynamics of root cap sloughing in Arabidopsis is regulated by peptide signaling. (2018)
  • Author(s): Shin C.L., Wangenheim, D. Herrmann, U., Wildhagen, M., Kulik, I., Kopf, A., Ishida, T., Olsson, V., Anker, M. K., Albert, M., Butenko, M., Felix, G., Sawa, S., Claassen, M., Friml, J., and Aalen, R. B.
  • Journal Title: Nature Plants Volume: 4 Issue: 8 Pages: 596-604
  • DOI: 10.1038/s41477-018-0212-z
  • Peer Reviewed
• [Journal Article] CLE14 peptide signaling in Arabidopsis root hair cell fate determination (2018)
  • Author(s): Naoto Hayashi, Takuya Tetsumura, Shinichiro Sawa, Takuji Wada, and Rumi Tominaga-Wada
  • Journal Title: Plant Biotechnology Volume: 35 Issue: 1 Pages: 17-22
  • DOI: 10.5511/plantbiotechnology.18.0122a
  • NAID: 130006727675
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed / Open Access
• [Journal Article] Artificial Cultivation System for Gastrodia spp. and Identification of Associated Mycorrhizal Fungi. (2017)
  • Author(s): Shimaoka, C., Fukunaga, H., Inagaki, S., and Sawa, S.
  • Journal Title: J. Biol. Volume: 9 Issue: 4 Pages: 27-34
  • DOI: 10.5539/ijb.v9n4p27
  • Peer Reviewed
• [Journal Article] A Collection of Mutants for CLE-Peptide-Encoding Genes in Arabidopsis Generated by CRISPR/Cas9-Mediated Gene Targeting (2017)
  • Author(s): Yamaguchi Yasuka L、Ishida Takashi、Yoshimura Mika、Imamura Yuko、Shimaoka Chie、Sawa Shinichiro
  • Journal Title: Plant and Cell Physiology Volume: 58 Issue: 11 Pages: 1848-1856
  • DOI: 10.1093/pcp/pcx139
  • Peer Reviewed / Open Access
• [Journal Article] Insights into Land Plant Evolution Garnered from the Marchantia polymorpha Genome. (2017)
  • Author(s): Bowman J, Kouchi T,..(106名）..Yokoyama R, ..(4名)
  • Journal Title: Cell Volume: 171 Issue: 2 Pages: 287-304
  • DOI: 10.1016/j.cell.2017.09.030
  • NAID: 120006353839
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Root-knot and cyst nematodes activate procambium-associated genes in Arabidopsis roots (2017)
  • Author(s): Yasuka L Yamaguchi, Reira Suzuki, Javier Cabrera, Satoru Nakagami, Tomomi Sagara, Chika Ejima, Ryosuke Sano, Yuichi Aoki, Rocio Olmo Lopez, Tetsuya Kurata, Takeshi Obayashi, Taku Demura, Takashi Ishida, Carolina Escobar, Shinichiro Sawa
  • Journal Title: Frontiers in Plant Science Volume: 8 Pages: 1195-1195
  • DOI: 10.3389/fpls.2017.01195
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Negative phototaxis in M. incognita (2017)
  • Author(s): Oota M, Gotoh E, Endo M, Ishida T, Matsushita T, and Sawa S
  • Journal Title: International Journal of Biology Volume: 9 Issue: 3 Pages: 12-16
  • DOI: 10.5539/ijb.v9n3p51
  • Peer Reviewed
• [Journal Article] Chloroplastic ATP synthase builds up proton motive force for preventing reactive oxygen species production in photosystemI. (2017)
  • Author(s): Takagi D., Amako, K., Hashiguchi M., Fukaki, H., Ishizaki, K., Goh T., Fukao Y., Sano, R., Kurata, T., Demura, T., Sawa, S., and Miyake C.
  • Journal Title: Plant J. Volume: 91 Issue: 2 Pages: 306-324
  • DOI: 10.1111/tpj.13566
  • Peer Reviewed
• [Presentation] Systemic long distance signal supports successful infection of plant parasitic root-knot nematodes, M. incognita (2019)
  • Author(s): 澤 進一郎
  • Organizer: International workshop of plants and nematodes interaction
  • Int'l Joint Research / Invited
• [Presentation] Plant attractant and systemic-peptide signaling supporting nematode successful infection. (2019)
  • Author(s): 澤 進一郎
  • Organizer: Mechanisms of Plant Perception -From endogenous to exogenous Lausanne
  • Int'l Joint Research / Invited
• [Presentation] Plant attractant and systemic peptide signaling supporting nematode successful infection (2019)
  • Author(s): 澤 進一郎
  • Organizer: Department seminar; Horticulture biology and metabolomics center
  • Int'l Joint Research / Invited
• [Presentation] Evolution of peptide signalling pathways in land plants (2019)
  • Author(s): Chihiro Furumizu, Shinichiro Saw
  • Organizer: The Vassilios Sarafis OzBryo Meeting
  • Int'l Joint Research
• [Presentation] マイクロチャンバーを用いた植物寄生性線虫の集団行動の分析 (2019)
  • Author(s): 肥田博隆, 森仁志, 神野伊策, 古水千尋, 澤進一郎
  • Organizer: 化学とマイクロ・ナノシステム学会 第４０回研究会
• [Presentation] Systemic long distance signal supports successful infection of plant parasitic root-knot nematodes, M. incognita (2018)
  • Author(s): Shinichiro Sawa
  • Organizer: International workshop of plants and nematodes interaction
  • Int'l Joint Research / Invited
• [Presentation] ネコブセンチュウ感染抵抗性トマトの原因究明と応用 (2018)
  • Author(s): 澤 進一郎
  • Organizer: くまもと産業復興支援プロジェクトフォーラム
  • Invited
• [Presentation] サツマイモネコブセンチュウの誘引物質の解析 (2018)
  • Author(s): 澤 進一郎
  • Organizer: 日本線虫学会大会公開シンポジウム
  • Invited
• [Presentation] 植物規制性線虫-植物間コミュニケーションの実態解明 (2018)
  • Author(s): 澤 進一郎
  • Organizer: 第22回天然薬物の開発と応用シンポジウム
  • Invited
• [Presentation] SpCas9 改変体を用いたシロイヌナズナゲノム編集 (2018)
  • Author(s): 山本晃大
  • Organizer: 第59回日本植物生理学会
• [Presentation] CLE-CLV1 は長距離シグナルを介して線虫感染を制御する (2018)
  • Author(s): 中上 知
  • Organizer: 第59回日本植物生理学会
• [Presentation] ペプチドホルモンCLE14がシロイヌナズナの根毛形成機構に及ぼす影響 (2018)
  • Author(s): 林直人
  • Organizer: 日本農芸化学会中四国支部第50回記念講演会
• [Presentation] サツマイモネコブセンチュウの根こぶ形成におけるオーキシンシグナリングと前形成層発生メカニズムの関与 (2018)
  • Author(s): 鈴木 れいら、澤 進一郎
  • Organizer: 第59 回日本植物生理学会
• [Presentation] 植物感染性線虫の植物感染に関わるホルモンの役割 (2017)
  • Author(s): 澤 進一郎
  • Organizer: ConBio2017
  • Int'l Joint Research / Invited
• [Presentation] Molecular mechanisms of gall development during plant parasitic nematode, M. incognita infection (2017)
  • Author(s): 澤 進一郎
  • Organizer: GDRI meeting 2017
  • Int'l Joint Research / Invited
• [Presentation] サツマイモネコブセンチュウに対する誘引物質の探索 (2017)
  • Author(s): 澤 進一郎
  • Organizer: 日本植物学会第81 回大会
  • Invited
• [Presentation] Release of novel root-knot nematode attractants through seed coat mucilage extrusion (2017)
  • Author(s): 澤 進一郎
  • Organizer: International workshop on Plant Parasitic Nematode in Kumamoto
  • Int'l Joint Research
• [Presentation] Regualtion of meristematic activity by CLE signaling pathway in Arabidopsis (2017)
  • Author(s): 澤 進一郎
  • Organizer: Plant Cell Signaling 2017
  • Int'l Joint Research / Invited
• [Remarks] The Sawa Lab.
  • URL: http://www.sci.kumamoto-u.ac.jp/~sawa/
• [Patent(Industrial Property Rights)] ピロール化合物を有効成分として含有する農園芸用殺菌剤または医用抗真菌剤 (2019)
  • Inventor(s): 谷時雄; 石川勇人; 澤進一郎; 石田喬志 他４名
  • Industrial Property Rights Holder: 谷時雄; 石川勇人; 澤進一郎; 石田喬志 他４名
  • Industrial Property Rights Type: 特許
  • Filing Date: 2019
• [Patent(Industrial Property Rights)] 植物成長抑制剤、およびそれを用いた植物成長抑制方法 (2018)
  • Inventor(s): 石川勇人、谷時雄、澤進一郎、石田喬志
  • Industrial Property Rights Holder: 石川勇人、谷時雄、澤進一郎、石田喬志
  • Industrial Property Rights Type: 特許
  • Filing Date: 2018