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Analyses of plant parasitic nematode infection mechanisms

Research Project

Project/Area Number 17H03967
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionKumamoto University

Principal Investigator

Sawa Shinichiro  熊本大学, 大学院先端科学研究部(理), 教授 (00315748)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Keywords植物感染性線虫 / 植物感染性センチュウ / サツマイモネコブセンチュウ
Outline of Final Research Achievements

Seed or root exudate components are known to regulate nematode behaviours, however currently it’s unclear how plant affect the spermosphere or rhizosphere microbiota composition. Here we showed compounds associated with the seed coat mucilage or root exudates can regulate the behaviour of soil-borne pathogens, the root-knot nematodes, M. incognita.
We found that flax seed exudate is capable of attracting RKN in a dose-dependent manner. The RKN attractant could be purified from flax mucilage and consists of a hydrophilic carbohydrate derivatives. On the other hand, we screened a chemical library of synthetic compound for M. incognita attractants. M. incognita were found to be attracted specifically by natural compounds that possess three to five methylene groups between two terminal amino groups. Using cryo-TOF-SIMS/SEM, one of the diamine was indeed detected in soybean root cortex cells and the surrounding rhizosphere, establishing a chemical gradient.

Academic Significance and Societal Importance of the Research Achievements

本研究により、線虫の植物誘引物質が同定出来た。また、植物への感染過程における分子機構の一端も明らかになった。これらの情報は、学術的には、植物-動物相互作用の分子機構が明らかになっただけでなく、どのようにパラサイトーホストの関係が進化してきたかを紐解く鍵になると考えられる。また、これらの情報は、線虫のトラップ剤の開発や、線虫に抵抗性を持つ作物品種の開発にも繋がると考えられ、基礎研究だけでなく、応用研究分野にも、今後、広く、利用されると考えられる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (44 results)

All 2019 2018 2017 Other

All Int'l Joint Research (4 results) Journal Article (19 results) (of which Int'l Joint Research: 9 results,  Peer Reviewed: 19 results,  Open Access: 7 results) Presentation (18 results) (of which Int'l Joint Research: 9 results,  Invited: 11 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Int'l Joint Research] INRA/ENS Lyon(フランス)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] UNIVERSIDAD DE CASTILLA LA MANCHA(スペイン)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] INRA/ENS Lyon(フランス)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] UNIVERSIDAD DE CASTILLA LA MANCHA(スペイン)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Effect of the CLE14 polypeptide on <i>GLABRA2</i> homolog gene expression in rice and tomato roots2019

    • Author(s)
      Hayashi, N., Rongkavilit, N., Tetsumura, T., Sawa, S., Wada, T., and Tominaga-Wada, R.
    • Journal Title

      Plant Biotechnology

      Volume: 36 Issue: 3 Pages: 205-208

    • DOI

      10.5511/plantbiotechnology.19.0725a

    • NAID

      130007724770

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2019-09-25
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A new forma of the mycoheterotrophic orchid Lechanorchis japonica from Japan2019

    • Author(s)
      Suetsugu K., Kaida, S., Fukunaga, H., and Sawa, S.
    • Journal Title

      Acta Phytotax. Geobot.

      Volume: In Press

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The RopGEF KARAPPO Is Essential for the Initiation of Vegetative Reproduction in Marchantia polymorpha2019

    • Author(s)
      Hiwatashi T., Goh H., Yasui Y., Koh L.Q., Takami H., Kajikawa M., Kirita H., Kanazawa T., Minamino N., Togawa T., Sato M., Wakazaki M., Yamaguchi K., Shigenobu S., Fukaki H., Mimura T., Toyooka K., Sawa S., Yamato K.T.,Ueda T., Urano D., Kohchi T., Ishizaki K.
    • Journal Title

      Current Biology

      Volume: 29 Issue: 20 Pages: 3525-3531

    • DOI

      10.1016/j.cub.2019.08.071

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Developing heritable mutations in Arabidopsis thaliana using a modified CRISPR/Cas9 toolkit comprising PAM-altered Cas9 variants and gRNAs.2019

    • Author(s)
      Yamamoto, A., Ishida, T., Yoshimura, M., Kimura, Y., Sawa, S.
    • Journal Title

      Plant Cell Physiol

      Volume: 60 Issue: 10 Pages: 2255-2262

    • DOI

      10.1093/pcp/pcz118

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lecanorchis moritae (Orchidaceae, Vanilloideae), a new mycoheterotrophic species from Amami- Oshima Island, Japan, based on morphological and molecular data2019

    • Author(s)
      Suetsugu, K., Kaida, S., Hsu, T.-C., Sawa, S.
    • Journal Title

      Phytotaxa

      Volume: 404 Pages: 137-145

    • DOI

      10.5962/bhl.title.154048

    • NAID

      120006951628

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Diverse function of plant peptide hormones in local signaling and development2019

    • Author(s)
      Hirakawa Yuki、Sawa Shinichiro
    • Journal Title

      Current Opinion in Plant Biology

      Volume: 51 Pages: 81-87

    • DOI

      10.1016/j.pbi.2019.04.005

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Control of proliferation in the haploid meristem by CLE peptide signaling in Marchantia polymorpha.2019

    • Author(s)
      Hirakawa Y, Uchida N, Yamaguchi YL, Tabata R, Ishida S, Ishizaki K, Nishihama R, Kohchi T, Sawa S, Bowman JL
    • Journal Title

      PLoS Genet.

      Volume: 7 Issue: 3 Pages: e1007997-e1007997

    • DOI

      10.1371/journal.pgen.1007997

    • NAID

      120006621164

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Regulation of Root-Knot Nematode Behavior by Seed-Coat Mucilage-Derived Attractants.2019

    • Author(s)
      Tsai, A, Y-L., Higaki, T., Nguyen, C-N., Perfus-Barbeoch, L., Favery, B., and Sawa, S.
    • Journal Title

      Mol. Plants.

      Volume: 12 Issue: 1 Pages: 99-122

    • DOI

      10.1016/j.molp.2018.11.008

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] CLE9 peptide-induced stomatal closure is mediated by abscisic acid, hydrogen peroxide, and nitric oxide in Arabidopsis thaliana 5.発行年 2019年2019

    • Author(s)
      Zang, L., Shi, X., Zhang, Y., Wang, J., Yang, J., Ishida, T., Jiang, W., Han, X., Kang, J., Wang, X., Pan, L., Lv, S., Cao, B., Zhang, Y., Wu, J., Han, H., Hu, Z., Cui, L, Sawa, S., He, J., and Wang, G
    • Journal Title

      Plant Cell & Environment.

      Volume: 42 Issue: 3 Pages: 1033-1044

    • DOI

      10.1111/pce.13475

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The CLE9/10 secretory peptide regulates stomatal and vascular development through distinct receptors.2018

    • Author(s)
      Qian P, Song W, Yokoo T, Minobe A, Wang G, Ishida T, Sawa S, Chai Jijie; Kakimoto T.
    • Journal Title

      Nature Plants

      Volume: 4 Issue: 12 Pages: 1071-1081

    • DOI

      10.1038/s41477-018-0317-4

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 3D Body Evolution: Adding a New Dimension to Colonize the Land2018

    • Author(s)
      Furumizu, C., Hirakawa, Y., Bowman, J. L., and *Sawa, S.
    • Journal Title

      Curr. Biol.

      Volume: 28 Issue: 15 Pages: 838-840

    • DOI

      10.1016/j.cub.2018.06.040

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The dynamics of root cap sloughing in Arabidopsis is regulated by peptide signaling.2018

    • Author(s)
      Shin C.L., Wangenheim, D. Herrmann, U., Wildhagen, M., Kulik, I., Kopf, A., Ishida, T., Olsson, V., Anker, M. K., Albert, M., Butenko, M., Felix, G., Sawa, S., Claassen, M., Friml, J., and Aalen, R. B.
    • Journal Title

      Nature Plants

      Volume: 4 Issue: 8 Pages: 596-604

    • DOI

      10.1038/s41477-018-0212-z

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CLE14 peptide signaling in Arabidopsis root hair cell fate determination2018

    • Author(s)
      Naoto Hayashi, Takuya Tetsumura, Shinichiro Sawa, Takuji Wada, and Rumi Tominaga-Wada
    • Journal Title

      Plant Biotechnology

      Volume: 35 Issue: 1 Pages: 17-22

    • DOI

      10.5511/plantbiotechnology.18.0122a

    • NAID

      130006727675

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Artificial Cultivation System for Gastrodia spp. and Identification of Associated Mycorrhizal Fungi.2017

    • Author(s)
      Shimaoka, C., Fukunaga, H., Inagaki, S., and Sawa, S.
    • Journal Title

      J. Biol.

      Volume: 9 Issue: 4 Pages: 27-34

    • DOI

      10.5539/ijb.v9n4p27

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Collection of Mutants for CLE-Peptide-Encoding Genes in Arabidopsis Generated by CRISPR/Cas9-Mediated Gene Targeting2017

    • Author(s)
      Yamaguchi Yasuka L、Ishida Takashi、Yoshimura Mika、Imamura Yuko、Shimaoka Chie、Sawa Shinichiro
    • Journal Title

      Plant and Cell Physiology

      Volume: 58 Issue: 11 Pages: 1848-1856

    • DOI

      10.1093/pcp/pcx139

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Insights into Land Plant Evolution Garnered from the Marchantia polymorpha Genome.2017

    • Author(s)
      Bowman J, Kouchi T,..(106名)..Yokoyama R, ..(4名)
    • Journal Title

      Cell

      Volume: 171 Issue: 2 Pages: 287-304

    • DOI

      10.1016/j.cell.2017.09.030

    • NAID

      120006353839

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Root-knot and cyst nematodes activate procambium-associated genes in Arabidopsis roots2017

    • Author(s)
      Yasuka L Yamaguchi, Reira Suzuki, Javier Cabrera, Satoru Nakagami, Tomomi Sagara, Chika Ejima, Ryosuke Sano, Yuichi Aoki, Rocio Olmo Lopez, Tetsuya Kurata, Takeshi Obayashi, Taku Demura, Takashi Ishida, Carolina Escobar, Shinichiro Sawa
    • Journal Title

      Frontiers in Plant Science

      Volume: 8 Pages: 1195-1195

    • DOI

      10.3389/fpls.2017.01195

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Negative phototaxis in M. incognita2017

    • Author(s)
      Oota M, Gotoh E, Endo M, Ishida T, Matsushita T, and Sawa S
    • Journal Title

      International Journal of Biology

      Volume: 9 Issue: 3 Pages: 12-16

    • DOI

      10.5539/ijb.v9n3p51

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chloroplastic ATP synthase builds up proton motive force for preventing reactive oxygen species production in photosystemI.2017

    • Author(s)
      Takagi D., Amako, K., Hashiguchi M., Fukaki, H., Ishizaki, K., Goh T., Fukao Y., Sano, R., Kurata, T., Demura, T., Sawa, S., and Miyake C.
    • Journal Title

      Plant J.

      Volume: 91 Issue: 2 Pages: 306-324

    • DOI

      10.1111/tpj.13566

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] Systemic long distance signal supports successful infection of plant parasitic root-knot nematodes, M. incognita2019

    • Author(s)
      澤 進一郎
    • Organizer
      International workshop of plants and nematodes interaction
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Plant attractant and systemic-peptide signaling supporting nematode successful infection.2019

    • Author(s)
      澤 進一郎
    • Organizer
      Mechanisms of Plant Perception -From endogenous to exogenous Lausanne
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Plant attractant and systemic peptide signaling supporting nematode successful infection2019

    • Author(s)
      澤 進一郎
    • Organizer
      Department seminar; Horticulture biology and metabolomics center
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Evolution of peptide signalling pathways in land plants2019

    • Author(s)
      Chihiro Furumizu, Shinichiro Saw
    • Organizer
      The Vassilios Sarafis OzBryo Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マイクロチャンバーを用いた植物寄生性線虫の集団行動の分析2019

    • Author(s)
      肥田博隆, 森仁志, 神野伊策, 古水千尋, 澤進一郎
    • Organizer
      化学とマイクロ・ナノシステム学会 第40回研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Systemic long distance signal supports successful infection of plant parasitic root-knot nematodes, M. incognita2018

    • Author(s)
      Shinichiro Sawa
    • Organizer
      International workshop of plants and nematodes interaction
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ネコブセンチュウ感染抵抗性トマトの原因究明と応用2018

    • Author(s)
      澤 進一郎
    • Organizer
      くまもと産業復興支援プロジェクトフォーラム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] サツマイモネコブセンチュウの誘引物質の解析2018

    • Author(s)
      澤 進一郎
    • Organizer
      日本線虫学会大会公開シンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 植物規制性線虫-植物間コミュニケーションの実態解明2018

    • Author(s)
      澤 進一郎
    • Organizer
      第22回天然薬物の開発と応用シンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] SpCas9 改変体を用いたシロイヌナズナゲノム編集2018

    • Author(s)
      山本晃大
    • Organizer
      第59回日本植物生理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] CLE-CLV1 は長距離シグナルを介して線虫感染を制御する2018

    • Author(s)
      中上 知
    • Organizer
      第59回日本植物生理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ペプチドホルモンCLE14がシロイヌナズナの根毛形成機構に及ぼす影響2018

    • Author(s)
      林直人
    • Organizer
      日本農芸化学会中四国支部第50回記念講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] サツマイモネコブセンチュウの根こぶ形成におけるオーキシンシグナリングと前形成層発生メカニズムの関与2018

    • Author(s)
      鈴木 れいら、澤 進一郎
    • Organizer
      第59 回日本植物生理学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 植物感染性線虫の植物感染に関わるホルモンの役割2017

    • Author(s)
      澤 進一郎
    • Organizer
      ConBio2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular mechanisms of gall development during plant parasitic nematode, M. incognita infection2017

    • Author(s)
      澤 進一郎
    • Organizer
      GDRI meeting 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] サツマイモネコブセンチュウに対する誘引物質の探索2017

    • Author(s)
      澤 進一郎
    • Organizer
      日本植物学会第81 回大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Release of novel root-knot nematode attractants through seed coat mucilage extrusion2017

    • Author(s)
      澤 進一郎
    • Organizer
      International workshop on Plant Parasitic Nematode in Kumamoto
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regualtion of meristematic activity by CLE signaling pathway in Arabidopsis2017

    • Author(s)
      澤 進一郎
    • Organizer
      Plant Cell Signaling 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Remarks] The Sawa Lab.

    • URL

      http://www.sci.kumamoto-u.ac.jp/~sawa/

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
  • [Patent(Industrial Property Rights)] ピロール化合物を有効成分として含有する農園芸用殺菌剤または医用抗真菌剤2019

    • Inventor(s)
      谷時雄; 石川勇人; 澤進一郎; 石田喬志 他4名
    • Industrial Property Rights Holder
      谷時雄; 石川勇人; 澤進一郎; 石田喬志 他4名
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] 植物成長抑制剤、およびそれを用いた植物成長抑制方法2018

    • Inventor(s)
      石川勇人、谷時雄、澤進一郎、石田喬志
    • Industrial Property Rights Holder
      石川勇人、谷時雄、澤進一郎、石田喬志
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2018 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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