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New regulatory mechanisms of cellular Ca2+ signaling by chloroplasts

Research Project

Project/Area Number 17H03968
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionSetsunan University (2020-2021)
Kyoto Prefectural University (2017-2019)

Principal Investigator

Shiina Takashi  摂南大学, 農学部, 教授 (10206039)

Co-Investigator(Kenkyū-buntansha) 野村 裕也  岐阜女子大学, 家政学部, 准教授 (00547028)
熊崎 茂一  京都大学, 理学研究科, 准教授 (40293401)
石崎 陽子  摂南大学, 農学部, 助手 (50423869)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2020: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords葉緑体 / ミトコンドリア / Ca2+ / ROS / シグナル伝達 / ストレス応答 / 気孔 / カルシウム / サリチル酸 / 機械受容チャネル / Ca2+チャネル / 環境センサー / 免疫応答 / 感染防御 / 接触刺激 / レトログレードシグナル / 感染防御応答 / 光合成
Outline of Final Research Achievements

In mammalian cells, mitochondria play a vital role in maintaining intracellular calcium(Ca2+) homeostasis. In contrast, the role of symbiotic organelles such as chloroplasts and mitochondria in plant cellular Ca2+ signaling remains elusive. In this study, we characterized two chloroplast-localized Ca2+-binding proteins CAS and CRSH. We found that light-dependent phosphorylation of CAS mediated by thylakoid protein kinase STN8 is critical for the light-induced opening of stomata. In addition, CRSH is responsible for the darkness-induced accumulation of (p)ppGpp, which is necessary to downregulate some plastidial gene expression. Furthermore, we found that chloroplast-localized mechano-sensitive channels are involved in the regulation of cellular ROS localization. Moreover, we found that mitochondrial dysfunction leads to the generation of a cytosolic Ca2+ transient and subsequent activation of Ca2+-dependent stress gene expression.

Academic Significance and Societal Importance of the Research Achievements

植物の環境応答やストレス応答で、細胞内のCa2+シグナルと活性酸素種(ROS) シグナルは相互に作用しながら重要なシグナル伝達の役割を果たしている。植物細胞には葉緑体やミトコンドリアなどの細胞小器官が存在し、細胞質との間でCa2+やROS信号の伝達をしていると考えられるが、その分子機構はわかっていない。本研究では、葉緑体内でCa2+やROSシグナルに関わる遺伝子の機能を解析し明らかにした。また、ミトコンドリアによるCa2+シグナル制御の可能性も示した。

Report

(5 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (41 results)

All 2021 2020 2019 2018 2017

All Journal Article (10 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 10 results,  Open Access: 3 results) Presentation (30 results) (of which Int'l Joint Research: 7 results) Book (1 results)

  • [Journal Article] Mass Production of Virus-Like Particles Using Chloroplast Genetic Engineering for Highly Immunogenic Oral Vaccine Against Fish Disease.2021

    • Author(s)
      Nakahira Y, Mizuno K, Yamashita H, Tsuchikura M, Takeuchi K, Shiina T, Kawakami H
    • Journal Title

      Front Plant Sci.

      Volume: 12 Pages: 717952-717952

    • DOI

      10.3389/fpls.2021.717952

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Selective Activation of Chloroplast psbD Light-Responsive Promoter and psaA/B Promoter in Transplastomic Tobacco Plants Overexpressing Arabidopsis Sigma Factor AtSIG52020

    • Author(s)
      Mikio Nozoe, Yuichi Tsunoyama, Yoko Ishizaki, Yoichi Nakahira, Takashi Shiina
    • Journal Title

      Protein & Peptide Letters

      Volume: 27 Issue: 2 Pages: 168-175

    • DOI

      10.2174/0929866526666191014130605

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plastidial (p)ppGpp Synthesis by the Ca2+-Dependent RelA?SpoT Homolog Regulates the Adaptation of Chloroplast Gene Expression to Darkness in Arabidopsis2020

    • Author(s)
      Ono Sumire、Suzuki Sae、Ito Doshun、Tagawa Shota、Shiina Takashi、Masuda Shinji
    • Journal Title

      Plant and Cell Physiology

      Volume: 61 Issue: 12 Pages: 2077-2086

    • DOI

      10.1093/pcp/pcaa124

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of Bacterial and Fungal Communities in Soils under Different Farming Systems. The Cacao Plantation in Sulawesi Island―Indonesia2019

    • Author(s)
      Suwastika, I. N.; Cruz, A. F.; Pakawaru, N. A.; Wijayanti, W.; Muslimin; Basri, Z.; Ishizaki, Y.; Tanaka, T.; Ono, N.; Kanaya, S.; Shiina, T
    • Journal Title

      Eurasian Soil Sc.

      Volume: 52 Issue: 10 Pages: 1234-1243

    • DOI

      10.1134/s1064229319100144

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The Cacao plantation in Sulawesi Island – Indonesia: ;Characterization of the soil microbial community.2019

    • Author(s)
      I Negah Suwastika, Andre; Freire Cruz, Nurul Aisyah Pakawaru, Wahyuningsih Wijayanti, Muslimin, Zainuddin Basri, Yoko Ishizaki, Tetsushi Tanaka, Naoaki Ono, Shigehiko Kanaya, Takashi Shiina
    • Journal Title

      urasian Soil Science

      Volume: 印刷中

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Selective activation of chloroplast psbD light-responsive promoter and psaA/B promoter in transplastomic tobacco plants overexpressing Arabidopsis sigma factor AtSIG52019

    • Author(s)
      Mikio Nozoe, Yuichi Tsunoyama, Yoko Ishizaki, Yoichi Nakahira, Takashi Shiina
    • Journal Title

      Protein & Peptide Letters

      Volume: 印刷中

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Cacao plantation in Sulawesi Island, Indonesia:I an agro-ecological analysis of conventional and organic farms2018

    • Author(s)
      Andre; Freire Cruz, I Nengah Suwastika, Hayao Sasaki, Tomoharu Uchiyama, Nurul Aisyah Pakawaru, Wahyuningsih Wijayanti, Muslimin, Zainuddin Basri, Yoko Ishizaki, and Takashi Shiina
    • Journal Title

      Organic Agriculture

      Volume: 9 Issue: 2 Pages: 225-234

    • DOI

      10.1007/s13165-018-0224-z

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A Ycf2-FtsHi heteromeric AAA-ATPase complex is required for chloroplast protein import2018

    • Author(s)
      Kikuchi Shingo、Asakura Yukari、Imai Midori、Nakahira Yoichi、Kotani Yoshiko、Hashiguchi Yasuyuki、Nakai Yumi、Takafuji Kazuaki、B?dard Jocelyn、Hirabayashi-Ishioka Yoshino、Mori Hitoshi、Shiina Takashi、Nakai Masato
    • Journal Title

      The Plant Cell

      Volume: 30 Issue: 11 Pages: 2677-2703

    • DOI

      10.1105/tpc.18.00357

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Ferritin 2 domain-containing protein found in lacquer tree (Toxicodendron vernicifluum) sap has negative effects on laccase and peroxidase reactions.2017

    • Author(s)
      Kitajima, S., Imamura, T., Iibushi, J., Ikenaga, M., Tachibana, Y., Andoh, N., Oyabu, H., Hirooka, K., Shiina, T., Ishizaki, Y
    • Journal Title

      Biosci Biotechnol Biochem.

      Volume: 81 Issue: 6 Pages: 1165-1175

    • DOI

      10.1080/09168451.2017.1289814

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparative analysis of chloroplast psbD promoters in terrestrial plants2017

    • Author(s)
      Shimmura S., Nozoe, M., Kitora, S., Kin S., Matsutani, S., Ishizaki, Y., Nakahira, Y. and *Shiina, T
    • Journal Title

      Front. in Plant Sci

      Volume: 8 Pages: 1186-1186

    • DOI

      10.3389/fpls.2017.01186

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] シロイヌナズナの機械刺 激応答における活性酸素生成誘導2020

    • Author(s)
      田川 翔大,山岡 征矢,渡辺 拓也,豊田 正嗣,朽津 和幸, 椎名 隆
    • Organizer
      日本植物学会 第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Toxicodendron 属の葉緑体ゲノム塩基配列の解析と比較2020

    • Author(s)
      石崎 陽子,椎名 隆,北島 佐紀人
    • Organizer
      日本植物学会 第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 気孔応答におけるミトコンドリア機械受容チャネル MSL1 の役割2019

    • Author(s)
      藤森 達郎,泉田 颯太,渡辺 健太,椎名 隆
    • Organizer
      第83回植物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] マイハギ托葉の自律的旋回運動機構の解析2019

    • Author(s)
      後藤 遼子,高尾 実波,北島 佐紀人,石崎 陽子, 椎名 隆
    • Organizer
      第83回植物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] シロイヌナズナの気孔応答における Ca2+チャネル CNGC5 の役割の検証2019

    • Author(s)
      佐伯 雄亮,尾上 真里奈,水野 公貴,村田 鷹規,石崎 陽子, 椎名 隆
    • Organizer
      第83回植物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 光依存的気孔開口における葉緑体 Ca2+ 結 合タンパク質 CAS のリン酸化の役割2019

    • Author(s)
      上村優奈,桐島祐貴,水野公貴,石崎陽子, 椎名隆
    • Organizer
      第60回日本植物生理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ミトコンドリア阻害が誘導する細胞質ゾルの Ca2+濃度上昇による防御関連遺伝子の発現誘導2019

    • Author(s)
      村田鷹規,下谷紘司,小谷美穂,尾上真里奈,山崎加奈子,佐野智,椎名隆
    • Organizer
      第60回日本植物生理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 葉緑体タンパク質CASによる気 孔免疫応答の制御2018

    • Author(s)
      水野 公貴,上村 優奈,椎名 隆
    • Organizer
      第82回日本植物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] シロイヌナズナの CNGC 変異体の表現型解析2018

    • Author(s)
      尾上 真里奈,村田 鷹規,椎名 隆
    • Organizer
      第82回日本植物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 機械刺激による表皮細胞での ROS 生成の解析2018

    • Author(s)
      田川 翔大,山岡 征矢,渡辺 拓也,朽津 和幸,椎名 隆
    • Organizer
      第82回日本植物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ミトコンドリア内膜に局在する機械受容チャネル MSL1 の 気孔応答における役割2018

    • Author(s)
      渡辺 健太,泉田 颯太,椎名 隆
    • Organizer
      第82回日本植物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] シロイヌナズナの CAS リン酸化部位置換変異体におけるフ ラジェリン応答遺伝子の発現解析2018

    • Author(s)
      桐島 祐貴,上村 優奈,水野 公貴,石崎 陽子,椎名 隆
    • Organizer
      第82回日本植物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ウルシ及び近縁種の葉緑体ゲノムシークエンス2018

    • Author(s)
      石崎 陽子,桐島 祐貴,菅 裕,椎名 隆,北島 佐紀人
    • Organizer
      第82回日本植物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] DBMIB induced mitochondrial Ca2+ release and stress/defense gene expression2018

    • Author(s)
      Takaki Murata, Takanori Iwaki, Koji Shimotani, Miho Kotani, Kanako Yamasaki, Satoshi Sano, Takashi Shiina
    • Organizer
      ISPCB2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Role of Phosphorylation of Chloroplast Ca2+ Binding Protein CAS in the Regulation of Stomatal Movement2018

    • Author(s)
      Yuna Uemura, Yuki Kirishima, Masaki Mizuno, Koji Shimotani, Kanako Yamasaki, Yoko Ishizaki, Takashi Shiina
    • Organizer
      ISPCB2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulation of PAMP-induced ROS burst in stomatal immunity by Chloroplast protein CAS2018

    • Author(s)
      Masaki Mizuno, Yuna Uemura, Takashi Shiina
    • Organizer
      ISPCB2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 機械刺激に対する局所的な防御遺伝子の発現におけるNADPOHオキシダーゼの役割2018

    • Author(s)
      田川翔大,山岡征矢,渡辺拓也,椎名隆
    • Organizer
      第59回日本植物生理学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] オルガネラ電子伝達阻害と遺伝子発現応答2017

    • Author(s)
      村田 鷹規 , 下谷 講司 , 岩城 宇律, 小谷 美穂, 山崎 加奈子, 佐野 智, 椎名 隆
    • Organizer
      第81回日本植物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シロイヌナズナの機械刺激応答時におけるRbohの役割2017

    • Author(s)
      田川 翔大, 山岡 征矢, 渡辺 拓也, 椎名 隆
    • Organizer
      第81回日本植物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 葉緑体タンパク質CASによる細胞内Ca2+;応答の制御2017

    • Author(s)
      水野 公貴, 小谷 美穂, 上村 優奈, 椎名 隆
    • Organizer
      第81回日本植物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 葉緑体Ca2+結合タンパク質CASのリン酸化と気孔開閉制御2017

    • Author(s)
      上村 優奈 水野 公貴, 下谷 紘司, 山崎 加奈子, 椎名 隆
    • Organizer
      第81回日本植物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シロイヌナズナにおけるミトコンドリア機械刺激受容チャネルMSL1の解析2017

    • Author(s)
      泉田 颯太, 艾原 佐紀, 濱谷 昭寿, 椎名 隆
    • Organizer
      第81回日本植物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シロイヌナズナの葉緑体カルシウムの役割2017

    • Author(s)
      久保 有沙, 小谷 美穂, 上村 優奈, 椎名 隆
    • Organizer
      第81回日本植物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Possible role of phosphorylation of chloroplast Ca2+ binding protein CAS in the regulation of stomatal opening.2017

    • Author(s)
      Yuna Uemura, Masaki Mizuno, Koji Shimotani, Kanako Yamasaki, Yoko Ishizaki, Takashi Shiina
    • Organizer
      TJPB2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Organelle electron transfer inhibition and gene expression response2017

    • Author(s)
      Takaki Murata, Takanori Iwaki, Koji Shimotani, Miho Kotani, Kanako Yamasaki Satoshi Sano, Takashi Shiina
    • Organizer
      TJPB2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The role of mitochondrial mechanosensitive receptor channel MSL1 in seed germination in Arabidopsis thaliana2017

    • Author(s)
      Souta Izumida , Saki Yomogihara, Akitoshi Hamatani and Takashi Shiina
    • Organizer
      TJPB2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Tissue specific transcriptome analyses of Asian Lacquer tree2017

    • Author(s)
      Yoko Ishizaki Sakito Kitajima Yuki Kirishima Takashi Shiina
    • Organizer
      TJPB2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ca2+依存のミトコンドリアから核への レトログレードシグナルは防御関連応答の制御2017

    • Author(s)
      村田 鷹規 , 岩城 宇律, 下谷 講司 , 小谷 美穂, 山崎 加奈子, 佐野 智, 椎名 隆
    • Organizer
      第59回日本植物生理学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Ca2+による葉緑体機能制御の検討2017

    • Author(s)
      久保有沙,小谷美穂,村田鷹規、椎名隆
    • Organizer
      第59回日本植物生理学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] flg22誘導の気孔閉口における葉緑体タンパク質の役割2017

    • Author(s)
      水野公貴,上村優奈,小谷美穂,椎名隆
    • Organizer
      第59回日本植物生理学会
    • Related Report
      2017 Annual Research Report
  • [Book] Calcium transport elements in plants2021

    • Author(s)
      Nomura , H., Shiina, T.
    • Total Pages
      464
    • Publisher
      Elsevier
    • ISBN
      9780128217924
    • Related Report
      2020 Annual Research Report

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Published: 2017-04-28   Modified: 2023-01-30  

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