• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Functional and structural analyses of iterative type I polypektide synthases

Research Project

Project/Area Number 17H03995
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Natural medicines
Research InstitutionKitasato University (2021)
Iwate Medical University (2017-2020)

Principal Investigator

Fujii Isao  北里大学, 薬学部, 客員教授 (70181302)

Project Period (FY) 2017-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2021: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2020: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2019: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2018: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Keywordsポリケタイド / ポリケタイド合成酵素 / 生合成 / iPKS / 構造解析
Outline of Final Research Achievements

As a part of studies on fungal iterative type I polyketide synthases (iPKSs) to elucidate their reaction control mechanisms, the biosynthetic gene cluster of shimlactone was cloned from Emericella variecolor GF10 and analyzed. Although it was difficult to express ShmA protein in yeast, ShmA protein with His-tag was well expressed in Aspergillus oryzae and successfully purified using Ni-affinity chromatography. Thus obtained homogeneous ShmA iPKS protein paves the way to further studies on its three dimensional structural elucidation as an example of fungal iPKS.

Academic Significance and Societal Importance of the Research Achievements

糸状菌は様々なポリケタイド化合物を生産し、重要な医薬供給源となっている。本研究では、神経突起生成活性を有し特異なビシクロ環構造をもつシマラクトンについて、ポリケタイド合成酵素ShmAを含むシマラクトン生合成遺伝子クラスターを同定するとともにShmAタンパクの異種発現、精製法を確立した。これは今後のポリケタイド合成酵素を利用した生物合成系開発への足掛かりとなるものと考えられる。

Report

(6 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (20 results)

All 2021 2020 2019 2018 2017 Other

All Journal Article (7 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 7 results,  Open Access: 4 results) Presentation (10 results) (of which Invited: 1 results) Remarks (3 results)

  • [Journal Article] Monapinone Coupling Enzyme Produces Non-Natural Heterodimers2021

    • Author(s)
      Ohte Satoshi、Toyoda Masayuki、Kobayashi Keisuke、Fujii Isao、Ohshiro Taichi、Tomoda Hiroshi
    • Journal Title

      Catalysts

      Volume: 11 Issue: 8 Pages: 1015-1015

    • DOI

      10.3390/catal11081015

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Novel angular naphthopyrone formation by Arp1p dehydratase involved in <i>Aspergillus fumigatus</i> melanin biosynthesis2021

    • Author(s)
      Nambu Natsuki、Tsai Huei‐Fung、Chang Yun C.、Kwon‐Chung K. J.、Yoshida Tomoki、Tanaka Nobutada、Tomoda Hiroshi、Ebizuka Yutaka、Fujii Isao
    • Journal Title

      Environmental Microbiology Reports

      Volume: 13 Issue: 6 Pages: 822-829

    • DOI

      10.1111/1758-2229.13013

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Shimalactone Biosynthesis Involves Spontaneous Double Bicyclo‐Ring Formation with 8π‐6π Electrocyclization2020

    • Author(s)
      I. Fujii、M. Hashimoto、K. Konishi、A. Unezawa、H. Sakuraba、K. Suzuki、H. Tsushima、M. Iwasaki、S. Yoshida、A. Kudo、R. Fujita、A. Hichiwa、K. Saito、T. Asano、J. Ishikawa、D. Wakana、Y. Goda、A. Watanabe、M. Watanabe、Y. Masumoto、J. Kanazawa、H. Sato、M. Uchiyama
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 59 Issue: 22 Pages: 8464-8470

    • DOI

      10.1002/anie.202001024

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional expression of a highly-reducing polyketide synthase of Emericella variecolor IFM42010, an asteltoxin-producing strain, resulted in production of two polyenoic β-ketolactones with opposite stereochemistry2019

    • Author(s)
      Hashimoto Makoto、Ichijo Hitomi、Fujiwara Kotaro、Sugasawa Hitoshi、Abo Seika、Matsudo Kimihito、Uchiyama Nahoko、Goda Yukihiro、Fujii Isao
    • Journal Title

      Bioorganic & Medicinal Chemistry Letters

      Volume: 29 Issue: 24 Pages: 126686-126686

    • DOI

      10.1016/j.bmcl.2019.126686

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the biosynthetic gene cluster for himeic acid A, a ubiquitin-activating enzyme (E1) inhibitor, in Aspergillus japonicus2018

    • Author(s)
      Makoto Hashimoto, Hikaru Kato, Ayako Katsuki, Sachiko, Tsukamoto, Isao Fujii
    • Journal Title

      ChemBioChem

      Volume: 19 Issue: 6 Pages: 535-539

    • DOI

      10.1002/cbic.201700584

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] pH-Dependent production of himeic acid A and its non-enzymatic conversions to himeic acids B and C2018

    • Author(s)
      Ayako Katsuki, Hikaru Kato, Yurika Tahara, Makoto Hashimoto, Isao Fujii, Sachiko Tsukamoto
    • Journal Title

      Bioorg. Med. Chem.

      Volume: 26 Issue: 8 Pages: 1869-1874

    • DOI

      10.1016/j.bmc.2018.02.034

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Discovery of a Fungal Multicopper Oxidase That Catalyzes the Regioselective Coupling of a Tricyclic Naphthopyranone To Produce Atropisomers2018

    • Author(s)
      Mio Kawaguchi, Taichi Ohshiro, Masayuki Toyoda, Satoshi Ohte, Junji Inokoshi, Isao Fujii, Hiroshi Tomoda
    • Journal Title

      Angwandte Chemie Int. Edt.

      Volume: 57 Issue: 18 Pages: 5115-5119

    • DOI

      10.1002/anie.201800415

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] シマラクトン生合成遺伝子の酵母異種発現2020

    • Author(s)
      飛知和あいか、齋藤来春、橋元 誠、浅野 孝、藤井 勲
    • Organizer
      第140年会日本薬学会、京都
    • Related Report
      2019 Annual Research Report
  • [Presentation] シマラクトン生合成遺伝子の酵母異種発現系の構築2019

    • Author(s)
      齋藤来春、飛知和あいか、小野寺駿太、志賀勇貴、橋元 誠、浅野 孝、藤井 勲
    • Organizer
      第58回日本薬学会東北支部大会、仙台
    • Related Report
      2019 Annual Research Report
  • [Presentation] 糸状菌ポリケタイドの生物合成研究2019

    • Author(s)
      藤井 勲
    • Organizer
      第41回東北薬学セミナー、仙台
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Emericella variecolor GF10のメロテルペノイド生合成遺伝子クラスター2019

    • Author(s)
      内藤未早妃、工藤 萌、千葉 環、松川美保、宮 彩子、橋元 誠、林 宏明、藤井 勲
    • Organizer
      第138年会日本薬学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ユビキチン活性化酵素の選択的阻害剤himeic acid AのpH依存的生成とhimeic acids B, Cへの非酵素的変換2018

    • Author(s)
      勝木理子、田原由莉歌、加藤 光、橋元 誠、藤井 勲、塚本佐知子
    • Organizer
      第60回天然有機化合物討論回
    • Related Report
      2018 Annual Research Report
  • [Presentation] ゲノム編集技術CRISPR/Cas9を用いた糸状菌天然物の異種生産2018

    • Author(s)
      佐藤優哉、瀧野純矢、椎名哲也、鵜飼孝大、南篤志、尾﨑太郎、劉 成緯、藤井 勲、丸山潤一、及川英秋
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] CRISPR/Cas9システムを用いた糸状菌由来天然物の異種生産2018

    • Author(s)
      佐藤優哉、瀧野純矢、椎名哲也、鵜飼孝大、南 篤志、尾﨑太郎、劉 成偉、藤井 勲、丸山潤一、及川英秋
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Asteltoxin生産菌Emericella variecolor IFM42010由来ポリケタイド合成酵素遺伝子の機能解析2017

    • Author(s)
      橋元 誠、安保聖香、松戸公仁、佐藤紀恵、藤井 勲
    • Organizer
      第56年会日本薬学会東北支部大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 糸状菌Emericella variecolor IFM4210のasteltoxin生合成遺伝子2017

    • Author(s)
      橋元 誠、佐藤紀恵、藤井 勲
    • Organizer
      第17回糸状菌分子生物学コンファレンス
    • Related Report
      2017 Annual Research Report
  • [Presentation] 植物病原性糸状菌で見いだされたalternapyrone生合成遺伝子クラスターの機能解析2017

    • Author(s)
      佐藤優哉、南 篤志、熊倉直祐、Gan Pamela、尾崎太郎、劉 成緯、藤井 勲、白須 賢、及川英秋
    • Organizer
      第17回糸状菌分子生物学コンファレンス
    • Related Report
      2017 Annual Research Report
  • [Remarks] 岩手医科大学薬学部天然物化学分野ホームページ

    • URL

      http://inpc.iwate-med.ac.jp/iNPC_IMU/HOME.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] 岩手医科大学薬学部・天然物化学分野ホームページ

    • URL

      http://inpc.iwate-med.ac.jp/iNPC_IMU/HOME.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] 岩手医科大学薬学部天然物化学講座ホームページ

    • URL

      http://inpc.iwate-med.ac.jp

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi