| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2021: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2020: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2019: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2018: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Outline of Final Research Achievements |
As a part of studies on fungal iterative type I polyketide synthases (iPKSs) to elucidate their reaction control mechanisms, the biosynthetic gene cluster of shimlactone was cloned from Emericella variecolor GF10 and analyzed. Although it was difficult to express ShmA protein in yeast, ShmA protein with His-tag was well expressed in Aspergillus oryzae and successfully purified using Ni-affinity chromatography. Thus obtained homogeneous ShmA iPKS protein paves the way to further studies on its three dimensional structural elucidation as an example of fungal iPKS.
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