Synthesis and biological evaluation of selenoneine, naturally occurring selenium containing compound
| Project/Area Number |
17H04001
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental and hygienic pharmacy
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| Research Institution | Chiba University |
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Principal Investigator |
Suzuki Noriyuki 千葉大学, 大学院薬学研究院, 准教授 (10376379)
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| Co-Investigator(Kenkyū-buntansha) |
小椋 康光 千葉大学, 大学院薬学研究院, 教授 (40292677)
阿南 弥寿美 昭和薬科大学, 薬学部, 講師 (40403860)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2017: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
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| Keywords | セレニウム / 酸化ストレス / 金属毒性 / 栄養学 / 環境 |
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| Outline of Final Research Achievements |
The purpose of this study is to clarify the significance of the naturally occurring selenium containing compound, selenoneine, by analysis of physicochemical, biochemical, and biological properties in detail. For this purpose, the effect of selenoneine on the antioxidant ability of mammalian cells was examined by using selenoneine obtained from genetically modified Aspergillus soyae.
As a result, it was found that selenoneine suppressed the cytotoxicity of oxidative stress inducer on cultured cells in a dose-dependent manner. Because the protective effect against the oxidative stress did not depend on the exposure time of selenoneine, this protective effect was not due to the protein expressions derived from selenoneine as selenium source, but due to the intrinsic activity of this selenium-containing compound itself. This result was consistent with the results that selenoneine showed glutathione peroxidase-like activity.
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| Academic Significance and Societal Importance of the Research Achievements |
セレンは欠乏によって死に至る必須微量元素である一方で、過剰症による死亡例も報告されている毒性元素でもある。経管栄養などの通常の食事を摂取できない患者へのセレンの補充にはその用量や投与する化学形態に関して注意が必要である。本研究の酸化ストレスに対する細胞保護効果や細胞増殖促進作用の検討において、亜セレン酸はセレン栄養源として効果的に作用すると共に濃度依存的に細胞毒性の発現が認められた。その一方で、セレノネインは細胞保護効果や増殖促進効果のみが認められ、細胞毒性は確認されなかった。以上のことからセレノネインは、高用量においても毒性を示さない、安全なセレン栄養源としての可能性を有していると言える。
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Report
(4 results)
Research Products
(54 results)
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[Journal Article] Preparation of envelope-type lipid nanoparticles containing gold nanorods for photothermal cancer therapy.2017
Author(s)
W. K. D. Paraiso, H. Tanaka, Y. Sato, D. Shirane, N. Suzuki, Y. Ogra, K. Tange, Y. Nakai, H. Yoshioka, H. Harashima, H. Akita
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Journal Title
Colloid. Surf. B: Biointerfaces
Volume: 160
Pages: 715-723
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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