• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of novel probes for fluorescence polarization and their application in cell biology

Research Project

Project/Area Number 17H04013
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including histology/embryology)
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Terada Sumio  東京医科歯科大学, 大学院医歯学総合研究科, 教授 (00262022)

Co-Investigator(Kenkyū-buntansha) 川岸 将彦  東京医科歯科大学, 大学院医歯学総合研究科, 助教 (60323606)
齊藤 健太  東京医科歯科大学, 大学院医歯学総合研究科, 助教 (60374659)
佐藤 啓介  東京医科歯科大学, 大学院医歯学総合研究科, 助教 (60644044)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2018: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
Keywords蛍光偏光 / 細胞骨格 / 細胞学 / 顕微分光法
Outline of Final Research Achievements

Monitoring molecular orientation is the key approach to study the spatial organization of molecular alignments within supramolecular complexes in living cells. Fluorescence polarization microscopy is a promising solution, but it is challenging to label target proteins with fluorescent probes. We have developed a genetically encoded probe for fluorescence polarization microscopy. Observation of living starfish embryos expressing our probe that specifically binds to actin revealed a novel 3D structure that had been escaping from previous observations.

Academic Significance and Societal Importance of the Research Achievements

開発に成功した新規標識法は汎用性が高く、この技術を利用することによって今後、蛍光偏光を利用した生命科学領域における計測や観測の試行の増加が見込まれる。また、この新規手法により、私たちのグループの場合の応用例のような、高い基礎学術的意義を有する知見が蓄積すれば、細胞生物学、生物物理学、発生生物学をはじめ、基礎生命科学から蛍光計測を利用する応用生命科学まで、広範囲の応用成果が見込まれ、その社会的意義は大きいと予想される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (16 results)

All 2020 2019 2018 Other

All Int'l Joint Research (3 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (10 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Int'l Joint Research] ウッズホール海洋生物学研究所(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ウッズホール海洋生物学研究所(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] ウッズホール海洋生物学研究所(米国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Genetically encoded orientation probes for F-actin for fluorescence polarization microscopy.2019

    • Author(s)
      Nakai N, Sato K, Tani T, Saito K, Sato F, Terada S.
    • Journal Title

      Microscopy

      Volume: 68 Issue: 68 Pages: 359-368

    • DOI

      10.1093/jmicro/dfz022

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 新規ユニバーサル蛍光偏光プローブPOLArISの開発.2020

    • Author(s)
      佐藤啓介, 杉崎綾奈, 斎藤健太, Mehta Shalin, 白水美香子, 谷知己, 寺田純雄.
    • Organizer
      第125回 日本解剖学会総会・全国学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 蛍光偏光ライブイメージングによるヒトデ卵の初期発生におけるアクチン動態の解析.2020

    • Author(s)
      杉崎綾奈, 佐藤啓介, 千葉和義, 川岸将彦, 寺田純雄.
    • Organizer
      第125回 日本解剖学会総会・全国学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 蛍光偏光ライブイメージングのためのプローブPOLArISの汎用性拡張の試み.2020

    • Author(s)
      中井紀, 佐藤啓介, 杉崎綾奈, 永嶋一貴, 齊藤健太, 川岸将彦, 谷知己, 寺田純雄.
    • Organizer
      第125回 日本解剖学会総会・全国学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] GFP-based F-actin Orientation Probes for Fluorescence Polarization Microscopy and Speckle F-actin Orientation Imaginag in Living Cells.2019

    • Author(s)
      N.Nakai,K.Sato,T.Tani,K.Saito,F.Sato,S.Terada.
    • Organizer
      2019 The American Society for Cell Biology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 蛍光偏光顕微鏡観察のための遺伝子にコードされたアクチンプローブの開発2019

    • Author(s)
      中井紀、佐藤文哉、佐藤啓介、谷知己、寺田純雄
    • Organizer
      第11回 脳統合機能研究センター・お茶の水ニューロサイエンス協会共催 若手インスパイアシンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 新規アクチン蛍光偏光プローブの開発2019

    • Author(s)
      杉崎綾奈、佐藤啓介、千葉和義、Mehta Shalin、谷知己、寺田純雄
    • Organizer
      第124回日本解剖学会総会・全国学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Development of genetically-encoded actin probes for fluorescence polarization microscopy2018

    • Author(s)
      Nori Nakai, Fumiya Sato, Keisuke Sato, Tomomi Tani, Sumio Terada
    • Organizer
      The American Society for Cell Biology Annual Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of genetically-encoded actin probes for fluorescence polarization microscopy2018

    • Author(s)
      Nori Nakai, Fumiya Sato, Keisuke Sato, Tomomi Tani, Sumio Terada
    • Organizer
      第70回日本細胞生物学会・第51回日本発生生物学会 合同大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 蛍光偏光顕微鏡観察のためのアクチンプローブPANDIAの改良2018

    • Author(s)
      中井紀、佐藤文哉、佐藤啓介、谷知己、寺田純雄
    • Organizer
      第123回日本解剖学会全国学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Toward deciphering the mystery of cytoskeletal dynamics. Keynote Speech2018

    • Author(s)
      寺田純雄
    • Organizer
      The 33rd Joint Annual Conference of Biomedical Science, Taiwan
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Patent(Industrial Property Rights)] (蛍光標識に関する新規手法)2020

    • Inventor(s)
      寺田純雄他4名
    • Industrial Property Rights Holder
      寺田純雄他4名
    • Industrial Property Rights Type
      特許
    • Filing Date
      2020
    • Related Report
      2019 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 抗原結合タンパク質と蛍光タンパク質または蛍光標識されるタグタンパク質との融合タンパク質2019

    • Inventor(s)
      寺田純雄、佐藤啓介、中井紀、齊藤健太、川岸将彦
    • Industrial Property Rights Holder
      寺田純雄、佐藤啓介、中井紀、齊藤健太、川岸将彦
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-034315
    • Filing Date
      2019
    • Related Report
      2018 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2023-03-23  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi