| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
|
|---|
| Outline of Final Research Achievements |
ATP receptor channel P2X2 shows voltage-dependent gating, in spite of the absence of canonical voltage-sensor in the molecule. We approached the molecular mechanisms by voltage-clamp fluorometry analysis using unnatural Fluorescent Amino Acid (fUAA). When fUAA was introduced at Ala337 by mutagenesis in the transmembrane domain 2 (TM2), voltage dependent fluorescence change was observed. As the change was linear in the recorded voltage range, it was shown that it is not due to conformational change of P2X2 protein but due to an electrochromic effect of fUAA, and that 337 position is located at a converged electric field. As it is known Phe44 in TM1 is close to Ala337 in the presence of ATP, and as we showed the importance of the interaction between Ala337 and Phe44 for activation by mutagenesis, it was suggested that the interaction between them in the strong electric field is voltage-dependent and that it underlies the voltage-dependent gating of P2X2.
|
