| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Outline of Final Research Achievements |
The research aimed at developing fluorescence probes for visualization of the spatiotemporal dynamics of catecholamines in the brain, which are important neurotransmitters that control brain functions. In order to develop catecholamine-binding domain for fluorescence probes, we decided to obtain anti-catecholamine antibodies and convert them to single-chain fragments. We performed molecular design and organic synthesis of catecholamine derivatives and provided them for the production of immunoconjugates. A large number of hybridomas were obtained after treatment of mice with the conjugates, and antibodies binding to catecholamines were found through subsequent screening. However, a problem with their binding selectivity to physiological catecholamines has also been revealed. The findings obtained in this research are expected to be useful for future development of promising fluorescent probes.
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