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Mechanism clarification of new symptoms in DFNA1 causing from constitutively active DAI1 mutants

Research Project

Project/Area Number 17H04042
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionKobe University

Principal Investigator

Ueyama Takehiko  神戸大学, バイオシグナル総合研究センター, 准教授 (80346254)

Co-Investigator(Kenkyū-buntansha) 坂口 博史  京都府立医科大学, 医学(系)研究科(研究院), 准教授 (00515223)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywords感音難聴 / DFNA1 / DIAPH1 / DIA1 / 遺伝性難聴 / 進行性難聴 / 血液異常 / 遺伝 / DIA1
Outline of Final Research Achievements

1. Noise exposure induced significantly decreased number of the ribbon synapse in hair cells in mice expressing a DIA1 mutant (p.R1213X) compared with control mice, suggesting that noise is one of factors leading to progressive hearing loss in DFNA1 patients.
2. In mice expressing the DIA1 mutant, the mutant was localized at the apical junctional complex (AJC) of hair cells, where showed morphological abnormalities by TEM. Thus, AJC is likely the main lesion in DFNA1.
3. In aged mice expressing the DIA1 mutant, size of the platelets was spread over a wider range, compared with control mice. Detailed studies analyzing the mechanism manifesting the phenotype are going on.

Academic Significance and Societal Importance of the Research Achievements

遺伝性感音難聴の一病型であるDFNA1の進行性難聴の発症機序を解明した。1. 主要病変部位は、変異体が局在する蝸牛有毛細胞の頂側結合である、2. 騒音により有毛細胞内のリボンシナプス数が減少する。
これらは、DFNA1の治療戦略上、大きな成果である。現在、引き続きDFNA1の治療薬開発を進めているが、本研究の継続・成功により、有効な治療薬のない感音難聴の治療法開発の扉を開けることが出来ると確信している。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (19 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (1 results) Journal Article (12 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 12 results,  Open Access: 9 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Int'l Joint Research] Seoul University(韓国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Rac-Dependent Signaling from Keratinocytes Promotes Differentiation of Intradermal White Adipocytes2020

    • Author(s)
      Ueyama Takehiko、Sakuma Megumi、Nakatsuji Mio、Uebi Tatsuya、Hamada Takeshi、Aiba Atsu、Saito Naoaki
    • Journal Title

      Journal of Investigative Dermatology

      Volume: 140 Issue: 1 Pages: 75-84.e6

    • DOI

      10.1016/j.jid.2019.06.140

    • NAID

      120006733143

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Differential disruption of autoinhibition and defect in assembly of cytoskeleton during cell division decide the fate of human DIAPH1-related cytoskeletopathy2019

    • Author(s)
      Kim Bong Jik、Ueyama Takehiko、Miyoshi Takushi、Lee Seungmin、Han Jin Hee、Park Hye-Rim、Kim Ah Reum、Oh Jayoung、Kim Min Young、Kang Yong Seok、Oh Doo Yi、Yun Jiwon、Hwang Sang Mee、Kim Nayoung K D、Park Woong-Yang、Kitajiri Shin-ichiro、Choi Byung Yoon
    • Journal Title

      Journal of Medical Genetics

      Volume: 56 Issue: 12 Pages: 818-827

    • DOI

      10.1136/jmedgenet-2019-106282

    • NAID

      120007032326

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Spinocerebellar ataxia type 14 caused by a nonsense mutation in the PRKCG gene2019

    • Author(s)
      Shirafuji Toshihiko、Shimazaki Haruo、Miyagi Tatsuhiro、Ueyama Takehiko、Adachi Naoko、Tanaka Shigeru、Hide Izumi、Saito Naoaki、Sakai Norio
    • Journal Title

      Molecular and Cellular Neuroscience

      Volume: 98 Pages: 46-53

    • DOI

      10.1016/j.mcn.2019.05.005

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rho-family small GTPases: from highly polarized sensory neurons to cancer cells2019

    • Author(s)
      Ueyama T
    • Journal Title

      Cells

      Volume: 8 Issue: 2 Pages: 92-92

    • DOI

      10.3390/cells8020092

    • NAID

      120006599240

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Roles of Cdc42 and Rac in Bergmann glia during cerebellar corticogenesis2018

    • Author(s)
      Sakamoto I, Ueyama T, Hayashibe M, Nakamura T, Mohri H, Kiyonari H, Shigyo M, Tohda C, Saito N
    • Journal Title

      Exp. Neurol.

      Volume: 302 Pages: 57-67

    • NAID

      120006733139

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Hearing vulnerability after noise exposure in a mouse model of reactive oxygen species overproduction2018

    • Author(s)
      Morioka Shigefumi、Sakaguchi Hirofumi、Yamaguchi Taro、Ninoyu Yuzuru、Mohri Hiroaki、Nakamura Takashi、Hisa Yasuo、Ogita Kiyokazu、Saito Naoaki、Ueyama Takehiko
    • Journal Title

      Journal of Neurochemistry

      Volume: 146 Issue: 4 Pages: 459-473

    • DOI

      10.1111/jnc.14451

    • NAID

      120006545101

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pharmacological induction of heat shock proteins ameliorates toxicity of mutant PKCγ in spinocerebellar ataxia type 142018

    • Author(s)
      Nakazono, A., Adachi, N., Takahashi, H., Seki, T., Hamada, D., Ueyama, T., Sakai, N. and Saito, N.
    • Journal Title

      J. Biol. Chem.

      Volume: 293 Issue: 38 Pages: 14758-14774

    • DOI

      10.1074/jbc.ra118.002913

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Role of Cysteine String Protein Alpha (CSPα) Phosphorylation at Serine 10, and 34, by Protein Kinase Cγ for Presynaptic Maintenance.2018

    • Author(s)
      Shirafuji T, Ueyama T, Adachi N, Yoshino KI, Sotomaru Y, Uwada J, Kaneoka A, Ueda T, Tanaka S, Hide I, Saito N, Sakai N
    • Journal Title

      J. Neurosci.

      Volume: 38 Issue: 2 Pages: 278-290

    • DOI

      10.1523/jneurosci.1649-17.2017

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of Cdc42 and Rac in Bergmann glia during cerebellar corticogenesis. Exp. Neurol.2018

    • Author(s)
      Sakamoto I, Ueyama T, Hayashibe M, Nakamura T, Mohri H, Kiyonari H, Shigyo M, Tohda C, Saito N.
    • Journal Title

      Exp. Neurol.

      Volume: 302 Pages: 57-67

    • DOI

      10.1016/j.expneurol.2017.12.003

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Novel role of Rac-Mid1 signaling in medial cerebellar development.2017

    • Author(s)
      Nakamura T, Ueyama T, Ninoyu Y, Sakaguchi H, Choijookhuu N, Hishikawa Y, Kiyonari H, Kohta M, Sakahara M, de Curtis I, Kohmura E, Hisa Y, Aiba A, Saito N.
    • Journal Title

      Development

      Volume: 144 Issue: 10 Pages: 1863-1875

    • DOI

      10.1242/dev.147900

    • NAID

      120006363303

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Golgi-Associated Protein Kinase C-ε Is Delivered to Phagocytic Cups: Role of Phosphatidylinositol 4-Phosphate2017

    • Author(s)
      Hanes Cheryl M.、D’Amico Anna E.、Ueyama Takehiko、Wong Alexander C.、Zhang Xuexin、Hynes W. Frederick、Barroso Margarida M.、Cady Nathaniel C.、Trebak Mohamed、Saito Naoaki、Lennartz Michelle R.
    • Journal Title

      J. Immunol

      Volume: 199 Issue: 1 Pages: 271-277

    • DOI

      10.4049/jimmunol.1700243

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Validation of Anti-CSPα, SNAP25, Tyrosine Hydroxylase, Ubiquitin, Cleaved Caspase 3, and pSer PKC Motif Antibodies for Utilization in Western Blotting2017

    • Author(s)
      Shirafuji T, Ueyama T, Tanaka S, Hide I, Saito N and Sakai N
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 50 Issue: 6 Pages: 177-180

    • DOI

      10.1267/ahc.17028

    • NAID

      130006285168

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] ROSによる聴覚伝達障害の発症メカニズム解明2020

    • Author(s)
      陳 林 毛利宏明 齋藤尚亮 上山健彦
    • Organizer
      第93回日本薬理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 遺伝子改変マウスからの疾患病態解明と治療法開発戦略へのフィードバック2019

    • Author(s)
      上山 健彦
    • Organizer
      第60回日本組織細胞化学会総会・学術集会および第13回日中合同組織細胞化学セミナー
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 難聴を生み出す活性酸素発生源細胞の同定とその機能・障害メカニズムの解明2019

    • Author(s)
      毛利 宏明、二之湯 弦、坂口 博史、齋藤 尚亮、上山 健彦
    • Organizer
      第60回日本組織細胞化学会総会・学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] DFNA1疾患モデルマウスを用いたDIA1分子の局在と病態メカニズムの解2019

    • Author(s)
      二之湯弦、坂口博史、陳林、毛利宏明、齋藤尚亮、上山健彦
    • Organizer
      第60回日本組織細胞化学会総会・学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 活性酸素種(ROS)過剰発現モデルマウスにおける聴覚機能解析と聴覚保護因子の検索2019

    • Author(s)
      森岡 繁文、坂口 博史、山口 太郎、二之湯 弦、毛利 宏明、中村 高志、久 育男、荻田 喜代一、齋藤 尚亮、上山 健彦
    • Organizer
      第60回日本組織細胞化学会総会・学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 脳・脊髄損傷後修復時のアストロサイトにおける新規シグナリング2017

    • Author(s)
      上山健彦
    • Organizer
      第58回日本組織細胞化学会総会・学術集会
    • Related Report
      2017 Annual Research Report
    • Invited

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Published: 2017-04-28   Modified: 2021-02-19  

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