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Pathophysiological modulation of high-grade malignant potentials of pancreato-biliary cancer, and its clinical application

Research Project

Project/Area Number 17H04057
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionHirosaki University

Principal Investigator

KIJIMA Hiroshi  弘前大学, 医学研究科, 教授 (90204859)

Co-Investigator(Kenkyū-buntansha) 後藤 慎太郎  弘前大学, 医学研究科, 助手 (00826901)
吉澤 忠司  弘前大学, 医学研究科, 助教 (70761071)
袴田 健一  弘前大学, 医学研究科, 教授 (30271802)
呉 雲燕  弘前大学, 医学研究科, 助教 (40636586)
猪口 貞樹  東海大学, 医学部, 教授 (60160008)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2019: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywords胆道癌 / 膵癌 / 上皮間葉転換 / 浸潤性増殖 / 癌微小環境 / 時計遺伝子 / 細胞・組織 / 腫瘍 / 高悪性度
Outline of Final Research Achievements

High-grade malignant potentials of pancreato-biliary cancer is characterized by the cancer microenvironment with rapid cancer cell growth, epithelial-mesenchymal transition (EMT) of cancer cells, and lymphatic/venous/perineural invasion. In this study, we clarified several pathological properties of pancreato-biliary cancer cells as follows: (1) Co-culture assay demonstrated that the mesenchymal stem cells affected the aggressive migration/invasion abilities of cancer cells. (2) Three-dimensional culture study revealed characteristic steps of lymphatic invasion. (3) Image analysis showed immature fibrosis (desmoplasia) and cancer-associated fibroblasts (CAF) played important roles in patients’ prognosis.

Academic Significance and Societal Importance of the Research Achievements

近年,癌細胞の上皮間葉転換 (epithelial- mesenchymal transition, EMT) が癌の増殖・進展に重要であることが唱えられている。今回の研究で,胆道癌・膵癌が高悪性度形質を示す微小環境特性が,早期浸潤病巣より認められる癌細胞の上皮間葉転換によることが明らかとなった。特に,癌細胞と間葉系幹細胞との直接的な相互作用が,癌の高悪性度に関連していることが示された点は,高悪性度形質の制御に向けた大きな成果と言える。一方で,今後は,胆道癌・膵癌の高悪性度形質を制御し,患者予後を改善させるような臨床応用に向けた研究が求められる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (23 results)

All 2019 2018 2017 Other

All Journal Article (15 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 15 results,  Open Access: 15 results) Presentation (6 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Remarks (2 results)

  • [Journal Article] Histopathological Characteristics of Pancreatic Cancer Stroma Induced by Neoadjuvant Chemotherapy.2019

    • Author(s)
      Goto S, Ishido K, Yoshizawa T, Haga T, Morohashi S, Wu Y, Seino H, Hakamada K, Kijima H.
    • Journal Title

      Biomed Res J.

      Volume: 3 Pages: 61-64

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Retinoic Acid-Inducible Gene-i and CXCL10 are Involved in Biliary Atresia.2019

    • Author(s)
      Kimura T, Imaizumi T, Yoshida T, Shimada T, Hayakari R, Kawaguchi S, Yoshida H, Kobayashi T, Hirabayashi T, Mizukami H, Kijima H, Hakamada K.
    • Journal Title

      Hirosaki Med J.

      Volume: 69 Pages: 86-94

    • NAID

      130007656021

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Type I Collagen Induces DEC Expression and Epithelial-Mesenchymal Transition (EMT) in Human Breast Cancer MCF-7 Cells.2019

    • Author(s)
      Tsugeno Y, Yoshizawa T, Wu Y, Goto S, Haga T, Suzuki T, Seino H, Morohashi S, Kijima H.
    • Journal Title

      BioMed Res J.

      Volume: 3 Pages: 125-131

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Investigating the association between radiological images and the pathology of rectal cancer treated with neoadjuvant chemotherapy.2019

    • Author(s)
      Morohashi S, Morohashi H, Seino H, Yoshizawa T, Haga T, Goto S, Wu Y, Sakamoto Y, Hakamada K, Kijima H
    • Journal Title

      Mol Clin Oncol.

      Volume: 11 Pages: 573-579

    • DOI

      10.3892/mco.2019.1931

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Neoangiogenesis of gastric submucosa-invasive adenocarcinoma2018

    • Author(s)
      Sasaki Hanae、Morohashi Satoko、Toba Takahito、Seino Hiroko、Yoshizawa Tadashi、Hirai Hideaki、Haga Toshihiro、Wu Yunyan、Kijima Hiroshi
    • Journal Title

      Oncology Letters

      Volume: 3 Pages: 3895-3900

    • DOI

      10.3892/ol.2018.9116

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Jaundice as an Initial Manifestation of Pancreatic Dedifferentiated Liposarcoma2018

    • Author(s)
      Tadashi Yoshizawa, Satoko Morohashi, Shintaro Goto, Toshihiro Haga, Hiroko Seino, Yunyan Wu, Kenichi Hakamada, Hiroshi Kijima
    • Journal Title

      Biomed Res J

      Volume: 2 Pages: 12-17

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] BHLH Transcription Factors DEC1 and DEC2: From Structure to Various Diseases2018

    • Author(s)
      Yunyan Wu, Hiroshi Kijima
    • Journal Title

      Biomed Res J

      Volume: 2 Pages: 28-33

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Toll-like receptor 3 signaling contributes to regional neutrophil recruitment in cultured human glomerular endothelial cells.2018

    • Author(s)
      Liu Q, Imaizumi T, Kawaguchi S, Aizawa T, Matsumiya T, Watanabe S, Tsugawa K, Yoshida H, Tsuruga K, Joh K, Kijima H, Tanaka H
    • Journal Title

      Nephron

      Volume: 139 Issue: 4 Pages: 349-358

    • DOI

      10.1159/000489507

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Epithelial-mesenchymal Transition (EMT) is Correlated with Patient's Prognosis of Lung Squamous Cell Carcinoma2018

    • Author(s)
      Aruga N, Kijima H, Masuda R, Onozawa H, Yoshizawa T, Tanaka M, Inokuchi S, Iwazaki M.
    • Journal Title

      Tokai J Exp Clin Med

      Volume: 43 Pages: 5-13

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expression of cancer-associated fibroblast markers in advanced colorectal cancer2018

    • Author(s)
      Nishishita Rie、Morohashi Satoko、Seino Hiroko、Wu Yunyan、Yoshizawa Tadashi、Haga Toshihiro、Saito Kensuke、Hakamada Kenichi、Fukuda Shinsaku、Kijima Hiroshi
    • Journal Title

      Oncol Lett.

      Volume: 15 Pages: 6195-6202

    • DOI

      10.3892/ol.2018.8097

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Impact of the histological phenotype of extrahepatic bile duct carcinoma2017

    • Author(s)
      Okano Kensuke、Yoshizawa Tadashi、Miura Takuya、Ishido Keinosuke、Kudo Daisuke、Kimura Norihisa、Wakiya Taichi、Wu Yunyan、Morohashi Satoko、Hakamada Kenichi、Kijima Hiroshi
    • Journal Title

      Mol Clin Oncol.

      Volume: 8 Pages: 54-60

    • DOI

      10.3892/mco.2017.1472

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Prognostic Impact of Extracapsular Lymph Node Invasion and Myofibroblastic Activity in Extrahepatic Bile Duct Cancer2017

    • Author(s)
      Yoshizawa Tadashi、Ishido Keinosuke、Saito Kensuke、Haga Toshihiro、Seino Hiroko、Wu Yunyan、Morohashi Satoko、Hakamada Kenichi、Kijima Hiroshi
    • Journal Title

      Clin Med Insights Pathol.

      Volume: 10 Pages: 1-7

    • DOI

      10.1177/1179555717729652

    • NAID

      120006583346

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] DEC1 and DEC2 Regulate Apoptosis of Human Prostate Adenocarcinoma LNCap Cells.2017

    • Author(s)
      Liu Q, Wu Y, Seino H, Yoshizawa T, Haga T, Morohashi S, Kijima H.
    • Journal Title

      Biomed Res J.

      Volume: 1 Pages: 6-11

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] <b>DEC1 promotes hypoxia-induced epithelial-mesenchymal transition (EMT) in human hepatocellular carcinoma </b><b>cells </b>2017

    • Author(s)
      Murakami Keishu、Wu Yunyan、Imaizumi Tadaatsu、 Aoki Yuka、Liu Qiang、Yan Xu、Seino Hiroko、Yoshizawa Tadashi、Morohashi Satoko、Kato Yukio、Kijima Hiroshi
    • Journal Title

      Biomedical Research

      Volume: 38 Issue: 4 Pages: 221-227

    • DOI

      10.2220/biomedres.38.221

    • NAID

      130005966980

    • ISSN
      0388-6107, 1880-313X
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] DEC2 expression antagonizes cisplatin-induced apoptosis in human esophageal squamous cell carcinoma2017

    • Author(s)
      Sato Hidenobu、Wu Yunyan、Kato Yukio、Liu Qiang、Hirai Hideaki、Yoshizawa Tadashi、Morohashi Satoko、Watanabe Jun、Kijima Hiroshi
    • Journal Title

      Mol Med Rep.

      Volume: 16 Issue: 1 Pages: 43-48

    • DOI

      10.3892/mmr.2017.6571

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Functional analysis of clock gene products DEC1 and DEC2, as bHLH transcription factors.2019

    • Author(s)
      Hiroshi Kijima.
    • Organizer
      International Conference on Biomedical and Cancer Research. SciTech Biomed-Cancer Sciences 2019.
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Microenvironment of Pancreatic Cancer and Clinicopathological Significance.2019

    • Author(s)
      Shintaro Goto, Tadashi Yoshizawa, Hiroko Seino, Toshihiro Haga, Satoko Morohashi, Hiroshi Kijima.
    • Organizer
      International Conference on Biomedical and Cancer Research. SciTech Biomed-Cancer Sciences 2019.
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Transrational study between pancreatic cancer stroma and clinical image.2019

    • Author(s)
      Shintaro Goto, Tadashi Yoshizawa, Kana Saito, Takuya Shimanaka, Hiroko Seino, Takanobu Akaishi, Toshihiro Haga, Satoko Morohashi, Hiroshi Kijima.
    • Organizer
      Hirosaki Medical Science Forum 2019.
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Correlations between contrast enhanced CT image and histopathological characteristics of the pancreatic cancer stroma.2019

    • Author(s)
      Shintaro Goto, Seino Hiroko, Tadashi Yoshizawa, Toshihiro Haga, Satoko Morohashi, Keinosuke Ishido, Kenichi Hakamada, Hiroshi Kijima.
    • Organizer
      第16回日本病理学会カンファレンス.
    • Related Report
      2019 Annual Research Report
  • [Presentation] 膵癌の造影CT画像と病理学的特性との関連解析.2019

    • Author(s)
      齋藤佳南, 後藤慎太郎, 吉澤忠司, 羽賀敏博, 清野浩子, 諸橋聡子, 鬼島 宏.
    • Organizer
      第108回日本病理学会総会.
    • Related Report
      2019 Annual Research Report
  • [Presentation] 浸潤性膵管癌における癌間質の特性解析.2019

    • Author(s)
      嶋中拓弥, 後藤慎太郎, 吉澤忠司, 羽賀敏博, 清野浩子, 諸橋聡子, 鬼島宏.
    • Organizer
      第108回日本病理学会総会.
    • Related Report
      2019 Annual Research Report
  • [Remarks] 弘前大学大学院医学研究科病理生命科学講座ホームページ

    • URL

      http://www.med.hirosaki-u.ac.jp/~patho2/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 弘前大学大学院医学研究科病理生命科学講座

    • URL

      http://www.med.hirosaki-u.ac.jp/~patho2/

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

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