| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2017: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Outline of Final Research Achievements |
Global spread of drug-resistant Plasmodium falciparum is a major obstacle to malaria treatment. Although the identification of drug resistance genes is crucial to efforts aimed at fighting resistant parasites, no effective approach has yet been developed. Here, we report genome-wide functional screening as a new approach to identify drug resistance genes in P. falciparum: genomic libraries of a drug-resistant strain are directly generated in drug-sensitive parasites and the drug resistance gene is then robustly identified from these libraries by drug screening. We successfully used this approach to identify multi-drug-resistant transporter 7, an ATP-binding cassette transporter, as a novel mefloquine resistance gene from a field-isolated parasite strain, thus demonstrating the practical utility of the method. Genome-wide functional screening will facilitate the identification of drug resistance genes and will contribute to the global fight against drug-resistant parasites.
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