Predicting the emergence of drug-resistant malaria: Development of a comprehensive laboratory evolution system with accelerated evolution system

• Project/Area Number: 17H04074
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: Juntendo University
• Principal Investigator: Mita Toshihiro 順天堂大学, 医学(系)研究科(研究院), 教授 (80318013)
• Co-Investigator(Kenkyū-buntansha): 平井 誠 順天堂大学, 医学部, 准教授 (50326849)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2018: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2017: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: マラリア / 薬剤耐性 / 進化 / 高度変異原虫 / ミューテータ / 実験室進化 / 実験進化

Outline of Final Research Achievements

We successfully produced protozoa in a few weeks that could survive and proliferate at the piperaquine concentration 6 times higher than that of susceptible strains. There were eight common mutations in these, including the N351I mutation in the chloroquine-resistant gene, crt. When this mutation was introduced into a susceptible strain, a slight decrease in susceptibility to piperaquine and a marked decrease in growth rate were observed. These indicate that the remaining mutation(s) may be involved in the decreased susceptibility and/or compensation of fitness. Further analysis is underway on the significance of these mutations to piperaquine resistance. In a validation study on real malaria samples, we has evidenced the emergence of artemisinin-resistant falciparum malaria in Uganda, which was the first report of the artemisinin resistance in Africa. We have also revealed that there was nearly half the resistance to mefloquine in Uganda.

Academic Significance and Societal Importance of the Research Achievements

本研究によってマラリア原虫が薬剤耐性を獲得するまでのプロセスをラボで迅速に解明する道が開けた。自然界に薬剤耐性マラリアが出現する前に起こる遺伝的な変化を解明することによって、流行地域における耐性が出現する前にアラートを出すことを可能とする。この段階で迅速に治療政策を変更することによって耐性が実際に出現することを防止できる。これによって新薬開発→耐性出現→新薬開発、という終わりないレースに終止符を打つ。2050年には抗生物質耐性病原体の蔓延による世界の死亡数はがん死亡数を上回るとされており、本研究構想が対策の実際面や開発に与える社会的波及面でのインパクトは高い。

Research Products

• [Int'l Joint Research] グル大学(ウガンダ)
• [Int'l Joint Research] オックスフォード大学(英国)
• [Int'l Joint Research] WHO(スイス)
• [Int'l Joint Research] デバイン大学(パプアニューギニア)
• [Int'l Joint Research] ウォルター・アンド・イライザ・ホール医学研究所(オーストラリア)
• [Int'l Joint Research]
• [Int'l Joint Research] グル大学(ウガンダ)
• [Int'l Joint Research] デバイン大学(パプアニューギニア)
• [Int'l Joint Research] オックスフォード大学(英国)
• [Int'l Joint Research] マヒドン大学(タイ)
• [Journal Article] Evidence of Artemisinin-Resistant Malaria in Africa. (2021)
  • Author(s): Balikagala B, Fukuda N, Ikeda M, Katuro OT, Tachibana SI, Yamauchi M, Opio W, Emoto S, Anywar DA, Kimura E, Palacpac NMQ, Odongo-Aginya EI, Ogwang M, Horii T, Mita T.
  • Journal Title: N Engl J Med. Volume: 385 Issue: 13 Pages: 1163-71
  • DOI: 10.1056/nejmoa2101746
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Derivatives of Dictyostelium differentiation-inducing factors suppress the growth of Plasmodium parasites in vitro and in vivo. (2021)
  • Author(s): Mita T, Hirai M, Maki Y, Nahar S, Yoshida N, Oshima Y, Kikuchi H, Kubohara Y.
  • Journal Title: Biochem Pharmacol Volume: - Pages: 114834-114834
  • DOI: 10.1016/j.bcp.2021.114834
  • Peer Reviewed / Open Access
• [Journal Article] Pb103 Regulates Zygote/Ookinete Development in Plasmodium berghei via Double Zinc Finger Domains. (2021)
  • Author(s): Hirai M, Maeta A, Mori T and Mita T.
  • Journal Title: Pathogens Volume: 10(12) Issue: 12 Pages: 1536-1536
  • DOI: 10.3390/pathogens10121536
  • Peer Reviewed / Open Access
• [Journal Article] Increase in the proportion of Plasmodium falciparum with kelch13 C580Y mutation and decline in pfcrt and pfmdr1 mutant alleles in Papua New Guinea. (2021)
  • Author(s): Yoshida N, Yamauchi M, Morikawa R, Hombhanje F, Mita T.
  • Journal Title: Malar J. Volume: 20 Issue: 1 Pages: 410-410
  • DOI: 10.1186/s12936-021-03933-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Isolation of Mutants With Reduced Susceptibility to Piperaquine From a Mutator of the Rodent Malaria Parasite Plasmodium berghei. (2021)
  • Author(s): Ikeda M, Hirai M, Tachibana SI, Mori T, Mita T.
  • Journal Title: Front Cell Infect Microbiol. Volume: - Pages: 672691-672691
  • DOI: 10.3389/fcimb.2021.672691
  • Peer Reviewed / Open Access
• [Journal Article] Fitness of sulfadoxine-resistant Plasmodium berghei harboring a single mutation in dihydropteroate synthase (dhps). (2021)
  • Author(s): Yamauchi M, Hirai M, Tachibana SI, Mori T, Mita T.
  • Journal Title: Acta Trop. Volume: - Pages: 106049-106049
  • DOI: 10.1016/j.actatropica.2021.106049
  • Peer Reviewed / Open Access
• [Journal Article] Ex vivo susceptibility of Plasmodium falciparum to antimalarial drugs in Northern Uganda (2021)
  • Author(s): Fukuda N, Tachibana S-I、Ikeda M、Sakurai-Yatsushiro M、Balikagala B、Katuro O、Yamauchi M、Emoto S、Hashimoto M、Yatsushiro S、Sekihara Makoto、et al
  • Journal Title: Parasitology International Volume: 81 Pages: 102277-102277
  • DOI: 10.1016/j.parint.2020.102277
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Malaria parasite species composition of Plasmodium infections among asymptomatic and symptomatic school-age children in rural and urban areas of Kinshasa, Democratic Republic of Congo. (2021)
  • Author(s): Nundu SS, Culleton R, Simpson SV, Arima H, Muyembe JJ, Mita T,Ahuka S, Yamamoto T.
  • Journal Title: Malar J. Volume: 20 Issue: 1 Pages: 389-389
  • DOI: 10.1186/s12936-021-03919-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Postulated Process of Axoneme Organization in the Male Gametogenesis of Malaria Parasite Plasmodium berghei (2020)
  • Author(s): Kubo-Irie Miyoko、Hirai Makoto、Irie Masaru、Mohri Hideo
  • Journal Title: Zoological Science Volume: 38 Issue: 2 Pages: 187-192
  • DOI: 10.2108/zs200064
  • NAID: 210000163224
  • Peer Reviewed / Open Access
• [Journal Article] Quantitative Detection of Plasmodium falciparum Using, LUNA-FL, A Fluorescent Cell Counter (2020)
  • Author(s): Hashimoto Muneaki、Yokota Kazumichi、Kajimoto Kazuaki、Matsumoto Musashi、Tatsumi Atsuro、Yamamoto Kenichi、Hyodo Tomonori、Matsushita Kiichiro、Minakawa Noboru、Mita Toshihiro、Oka Hiroaki、Kataoka Masatoshi
  • Journal Title: Microorganisms Volume: 8 Issue: 9 Pages: 1356-1356
  • DOI: 10.3390/microorganisms8091356
  • Peer Reviewed / Open Access
• [Journal Article] Development of a quantitative, portable, and automated fluorescent blue-ray device-based malaria diagnostic equipment with an on-disc SiO2 nanofiber filter (2020)
  • Author(s): Yamamoto Takeki、Hashimoto Muneaki、Nagatomi Kenji、Nogami Takahiro、Sofue Yasuyuki、Hayashi Takuya、Ido Yusuke、Yatsushiro Shouki、Abe Kaori、Kajimoto Kazuaki、Tamari Noriko、Awuor Beatrice、Sonye George、Kongere James、Munga Stephen、Ohashi Jun、Oka Hiroaki、Minakawa Noboru、Kataoka Masatoshi、Mita Toshihiro
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 6585-6585
  • DOI: 10.1038/s41598-020-63615-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Recovery and stable persistence of chloroquine sensitivity in Plasmodium falciparum parasites after its discontinued use in Northern Uganda (2020)
  • Author(s): Balikagala Betty、Sakurai-Yatsushiro Miki、Hirai Makoto et al
  • Journal Title: Malaria Journal Volume: 19 Issue: 1 Pages: 1-12
  • DOI: 10.1186/s12936-020-03157-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Highly Sensitive and Rapid Quantitative Detection of Plasmodium falciparum Using an Image Cytometer (2020)
  • Author(s): Hashimoto Muneaki、Yokota Kazumichi、Kajimoto Kazuaki、Matsumoto Musashi、Tatsumi Atsuro、Nakajima Yoshihiro、Mita Toshihiro、Minakawa Noboru、Oka Hiroaki、Kataoka Masatoshi
  • Journal Title: Microorganisms Volume: 8 Issue: 11 Pages: 1769-1769
  • DOI: 10.3390/microorganisms8111769
  • Peer Reviewed / Open Access
• [Journal Article] Emergence of artemisinin-resistant Plasmodium falciparum with kelch13 C580Y mutations on the island of New Guinea (2020)
  • Author(s): Miotto Olivo、Sekihara Makoto、Tachibana Shin-Ichiro、Yamauchi Masato et al
  • Journal Title: PLOS Pathogens Volume: 16 Issue: 12 Pages: e1009133-e1009133
  • DOI: 10.1371/journal.ppat.1009133
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Nucleic acid purification from dried blood spot on FTA Elute Card provides template for polymerase chain reaction for highly sensitive Plasmodium detection (2019)
  • Author(s): Hashimoto Muneaki、Bando Mika、Kido Jun-ichi、Yokota Kazumichi、Mita Toshihiro、Kajimoto Kazuaki、Kataoka Masatoshi
  • Journal Title: Parasitology International Volume: 73 Pages: 101941-101941
  • DOI: 10.1016/j.parint.2019.101941
  • Peer Reviewed / Open Access
• [Journal Article] Development of a highly sensitive, quantitative, and rapid detection system for Plasmodium falciparum-infected red blood cells using a fluorescent blue-ray optical system (2019)
  • Author(s): Yamamoto Takeki、Yatsushiro Shouki、Hashimoto Muneaki、Kajimoto Kazuaki、Ido Yusuke、Abe Kaori、Sofue Yasuyuki、Nogami Takahiro、Hayashi Takuya、Nagatomi Kenji、Minakawa Noboru、Oka Hiroaki、Mita Toshihiro、Kataoka Masatoshi
  • Journal Title: Biosensors and Bioelectronics Volume: 132 Pages: 375-381
  • DOI: 10.1016/j.bios.2019.02.064
  • Peer Reviewed / Open Access
• [Journal Article] See-through observation of malaria parasite behaviors in the mosquito vector (2019)
  • Author(s): Mori Toshiyuki、Hirai Makoto、Mita Toshihiro
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 1768-1768
  • DOI: 10.1038/s41598-019-38529-3
  • Peer Reviewed / Open Access
• [Journal Article] Plasmodium malariae and Plasmodium ovale infections and their association with common red blood cell polymorphisms in a highly endemic area of Uganda. (2019)
  • Author(s): Subissi L, Kanoi BN, Balikagala B, Egwang TG, Oguike M, Verra F, Proietti C, Bousema T, Drakeley CJ, Sepulveda N.
  • Journal Title: Trans R Soc Trop Med Hyg. Volume: 113 Issue: 7 Pages: 370-8
  • DOI: 10.1093/trstmh/trz015
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Rapid selection of sulphadoxine-resistant Plasmodium falciparum and its effect on within-population genetic diversity in Papua New Guinea. (2018)
  • Author(s): Mita T, Hombhanje F, Takahashi N, Sekihara M, Yamauchi M, Tsukahara T, Kaneko A, Endo H, Ohashi J. (Corresponding author)
  • Journal Title: Scientific reports. Volume: 8 Issue: 1 Pages: 5565-5565
  • DOI: 10.1038/s41598-018-23811-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Lack of significant recovery of chloroquine sensitivity in Plasmodium falciparum parasites following discontinuance of chloroquine use in Papua New Guinea (2018)
  • Author(s): Sekihara M, Tachibana S-I, Yamauchi M, Yatsushiro S, Tiwara S, Fukuda N, Ikeda M, Mori T, Hirai M, Hombhanje F, Mita T
  • Journal Title: Malaria Journal Volume: 17 Issue: 1 Pages: 434-434
  • DOI: 10.1186/s12936-018-2585-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 薬剤耐性マラリア原虫の進化 (2018)
  • Author(s): 美田敏宏
  • Journal Title: 生物の科学 遺伝 Volume: 72 Pages: 382-388
• [Journal Article] 薬剤耐性マラリアの最新疫学知見 (2018)
  • Author(s): 美田敏宏
  • Journal Title: 病原微生物検出情報 国立感染症研究所 Volume: 39 Pages: 173-174
• [Journal Article] 蚊媒介感染症に関する最近の話題 (2017)
  • Author(s): 美田敏宏
  • Journal Title: 臨床と微生物 Volume: 44 Pages: 263-268
• [Journal Article] Absence of in vivo selection for K13 mutations after artemether-lumefantrine treatment in Uganda. (2017)
  • Author(s): Balikagala B, Mita T, Ikeda M, Sakurai M, Yatsushiro S, Takahashi N, Tachibana SI, Auma M, Ntege EH, Ito D, Takashima E, Palacpac NM, Egwang TG, Onen JO, Kataoka M, Kimura E, Horii T, Tsuboi T.
  • Journal Title: Malar J. Volume: 16(1) Issue: 1 Pages: 1-23
  • DOI: 10.1186/s12936-016-1663-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] ネズミマラリア原虫Kelch13のインターラクトーム解析 (2021)
  • Author(s): 平井 誠、森 稔幸、美田 敏宏
  • Organizer: 第90回日本寄生虫学会大会
• [Presentation] 熱帯熱マラリア原虫－血管内皮間の接着と脂質代謝異常の関連 (2021)
  • Author(s): 福田 直到，Balikagala B，江本 桜子，上野 剛，Katuro O，Palacpac N，木村 英作，三井田 孝，堀井 俊宏，美田 敏宏
  • Organizer: 第90回日本寄生虫学会大会
• [Presentation] パプアニューギニアにおけるKelch 13 C580Y変異熱帯熱マラリア原虫の出現と現状 (2021)
  • Author(s): 吉田 菜穂子、山内 祐人、森川 瞭介、宋 書馨、平井 誠、美田 敏宏
  • Organizer: 第90回日本寄生虫学会大会
• [Presentation] Emergence of artemisinin resistance in Africa (2021)
  • Author(s): Betty Balikagala
  • Organizer: 第90回日本寄生虫学会大会
• [Presentation] Papua New Guinea におけるマラリア迅速検査の感度と特異度の解析 (2021)
  • Author(s): シジルエルデネ ムンフソブド、吉田 菜穂子、千葉 結奈、Francis Hombhanje、美田 敏宏
  • Organizer: 第 80 回日本寄生虫学会東日本支部大会
• [Presentation] 20年にわたるパプアニューギニアでのサルファドキシン・ピリメサミンの使用は薬剤耐性をもたらしたか？ (2021)
  • Author(s): 原田 治亮、吉田 菜穂子、山内 祐人、Francis Hombhanje、美田 敏宏
  • Organizer: 第 80 回日本寄生虫学会東日本支部大会
• [Presentation] 血清リポタンパクは熱帯熱マラリア原虫の増殖を促進するのか？ (2021)
  • Author(s): 宋 書馨、吉田 菜穂子、稲葉 悠、堀 敦詞、福田 直到、三井田 孝、美田 敏宏
  • Organizer: 第 80 回日本寄生虫学会東日本支部大会
• [Presentation] ビオチン化によって同定された、マラリア原虫メス側受精因子 (2020)
  • Author(s): 森 稔幸、平井 誠、美田 敏宏
  • Organizer: 第89回日本寄生虫学会誌上大会
• [Presentation] アルテミシニン耐性マーカーKelch13 ネズミマラリア原虫オルソログの機能解析 (2020)
  • Author(s): 平井 誠、森 稔幸、美田 敏宏
  • Organizer: 第89回日本寄生虫学会誌上大会
• [Presentation] 血管内皮との接着は熱帯熱マラリア原虫のアルテミシニン抵抗性を増強する (2020)
  • Author(s): 福田 直到，Balikagala B，山内祐人，牧 喜子，江本 桜子，落合 紗雪，Katuro O，橘 真一郎，Anywar D，Palacpac N，木村 英作，Odingo-Aginya E，三井田 孝，堀井 俊宏，美田 敏宏
  • Organizer: 第89回 日本寄生虫学会大会
• [Presentation] アフリカに出現したアルテミシニン耐性 (2020)
  • Author(s): 美田敏宏
  • Organizer: 日本熱帯医学会受賞記念講演
  • Invited
• [Presentation] In vivo artemisinin resistance has emerged in Uganda (Africa). (2020)
  • Author(s): Balikagala Betty,
  • Organizer: The United States-Japan Cooperative Medical Science Program. The 50th Joint Conference on Parasitic Diseases
  • Int'l Joint Research
• [Presentation] ビオチン化を利用した、マラリア原虫新規受精因子の探索 (2019)
  • Author(s): 森稔幸、平井誠、美田敏宏
  • Organizer: 第88回日本寄生虫学会大会
• [Presentation] ビオチン化を用いた、新規マラリア原虫赤血球侵入関連因子の探索 (2019)
  • Author(s): 落合紗雪、前田樹里、平井誠、美田敏宏、森稔幸
  • Organizer: 第88回日本寄生虫学会大会
• [Presentation] ウガンダにおけるマラリア予防政策とその実態 (2019)
  • Author(s): 片山瑞希、山内祐人、美田敏宏
  • Organizer: 第79回日本寄生虫学会東日本支部大会
• [Presentation] ウガンダ北部マラリア原虫集団におけるアルテミシニン耐性レベルの推移 (2019)
  • Author(s): 池田 美恵 , 金子 恵 , 橘 真一郎 , 山内 祐人 , バリカガラ ベティ, 江本 桜子 , 福田 直到 , 牧 喜子, 森 稔幸 , 平井 誠 , 橋本 宗明 , カツロ オスベルト , アウマ マリー, アニワー デニス , バラクパック ニリアン, 片岡 正俊, アギニア エマニュエル, 木村 英作, 堀井 俊宏 , 美田 敏宏
  • Organizer: 第88回日本寄生虫学会大会
• [Presentation] In-vivo artemisinin resistance may have emerged in Uganda, (2019)
  • Author(s): バリカガラ ベテイ , 池田 美恵 , 橘 真一郎 , 山内 祐人 , 福田 直到 , 関原 誠 , 江本 桜子 , Katuro Osbert T., Opio Walter, Auma Mary , Anywar Denis A. , 森 稔幸 ,平井 誠 , 片岡 正俊 , Palacpac Nirianne M.Q. , Odongo-Aginya Emmanuel I., 木村 英作 , 堀井 俊宏 , 美田 敏宏
  • Organizer: 第88回日本寄生虫学会大会
• [Presentation] 蚊-マウスモデルにおけるサルファドキシン耐性マラリア原虫の適応度に関する研究 (2019)
  • Author(s): 山内 祐人, 平井 誠, 橘 慎一郎, 森 稔幸, 美田 敏宏
  • Organizer: 第88回日本寄生虫学会大会
• [Presentation] マラリアの現状と薬剤耐性の最前線 (2019)
  • Author(s): 美田敏宏
  • Organizer: 日本細胞性粘菌学会第9回例会
  • Invited
• [Presentation] Synthetic biotin ligase enables to label surface invasion factors of cultured human malaria parasite, Plasmodium falciparum. (2019)
  • Author(s): Ochiai S, Maeda J, Hirai M, Mita T, Mori T.
  • Organizer: 68th Annual Meeting, Maryland USA
  • Int'l Joint Research
• [Presentation] Emergence of artemisinin-resistant P. falciparum with kelch13 C580Y mutations on the island of New Guinea. (2019)
  • Author(s): Miotto O, Sekihara M, Tachibana S, Yamauchi M, Pearson R, Amato R, Goncalves S, Somya M, Noviyanti R, Marfurt J, Auburn S, Price R, Mueller I, Ikeda M, Mori T, Hirai M, Tavul L, Hetzel M, Laman M, Barry A, Ringwald P, Ohashi J, Hombhanje F, Kwiatkowski D, Mita T
  • Organizer: American Society of Tropical Medicine and Hygiene Annual Meeting
  • Int'l Joint Research
• [Presentation] Epidemiology and evolution of artemisinin-resistant Plasmodium faliparum parasites. (2019)
  • Author(s): Mita T
  • Organizer: Asia - Pacific Scientific Workshop.
  • Int'l Joint Research
• [Presentation] Altered gut microbiota composition in Plasmodium falciparum patients in Uganda (2018)
  • Author(s): Taniguchi T. Miyauchi E. Olia A. Nagayasu E. Osbert K. Suzue K. Imai T. Shimokawa C. Onishi R. Odongo-Aginya EI. Palacpac N. Maruyama H. Kimura E. Mita T. Ohno H. Horii T.andHisaeda H.
  • Organizer: 14th International congress of parastiology
  • Int'l Joint Research
• [Presentation] Global issue of drug resistant malaria and population structure of malaria parasites for global malaria control (2018)
  • Author(s): Mita T. Tachibana S. Sekihara M. Yamauchi M. Hombhanje F.andOhashi J
  • Organizer: 14th International congress of parastiology
  • Int'l Joint Research
• [Presentation] Detection of Artemisinin-resistant Plasmodium falciparum with High Survival Rates in Uganda (2018)
  • Author(s): Mita T. Ikeda M. Kaneko M. Tachibana S. Balikagala B. Sakurai-Yatsushiro M. Yatsushiro S. Takahashi N. Yamauchi M. Sekihara M. Hashimoto M. Obwoya PS. Auma MA. Anywar DA. Odongo-Aginya EI. Hirai M. Ohashi J. Palacpac NMQ. Kataoka M. Tsuboi T. Kimura E.andHorii T
  • Organizer: 14th International congress of parastiology
  • Int'l Joint Research
• [Presentation] 原虫学・寄生虫学が現代のトップサイエンスにもたらす貢献 マラリアミューテーター マラリア原虫薬剤耐性進化解明の新たな研究ツール. (2017)
  • Author(s): 平井誠, 池田美恵, 橘真一郎, 美田敏宏.
  • Organizer: 生命科学系学会合同年次大会.
• [Presentation] End malaria-マラリアのeliminationをめざして アルテミシニン耐性の評価・検出ツールは出そろったか? 流行地でのフィールド調査から見えてきた問題点. (2017)
  • Author(s): 美田敏宏
  • Organizer: 生命科学系学会合同年次大会.
  • Invited
• [Presentation] 人類を助ける進化学研究：進化し続ける薬剤耐性マラリアの克服. (2017)
  • Author(s): 美田敏宏
  • Organizer: 公益信託 進化学振興木村資生基金 “微生物と人類の共存”をゲノムの視点から考える.
  • Invited
• [Presentation] ウガンダにおけるin vivo薬剤耐性試験およびRSAを用いたアルテミシニン耐性疫学調査. (2017)
  • Author(s): 池田美恵, 橘真一郎, 山内祐人, Auma M, 関原誠, 森稔幸, 平井誠, Palacpac N, 木村英作, Aginya E, 堀井俊宏, 美田敏宏.
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] ウガンダ国の熱帯熱マラリア患者において腸内細菌叢の変化が起こる. (2017)
  • Author(s): 谷口委代, 宮内栄治, OliaAlex, 長安英治, OsbertKaturo, 鈴江一友, 今井孝, 下川周子, 大西里咲, I. O-A, PalacpacNirianne, 丸山治彦, 木村英作, 美田敏宏, 大野博司, 堀井俊宏, 久枝一.
  • Organizer: グローバルヘルス合同大会プログラム・抄録集.
• [Presentation] ネズミマラリア原虫の有性生殖関連分子の機能解析. (2017)
  • Author(s): 前多晟壮, 森稔幸, 平井誠, 美田敏宏.
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] マラリア原虫有性生殖の透視観察法の開発 (2017)
  • Author(s): 森稔幸, 平井誠, 美田敏宏
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] ウガンダにおける熱帯熱マラリア原虫キニーネ感受性の実態. (2017)
  • Author(s): 山内祐人, 橘真一郎, 池田美恵, 関原誠, Balikagala B, 八代-櫻井美樹, 平井誠, Palacpac N, 木村英作, 堀井俊宏, 美田敏宏.
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] 線虫ミトコンドリアのプロテオーム解析 二次元電気泳動法とショットガン質量分析. (2017)
  • Author(s): 高宮信三郎, 數野彩子, 峯木礼子, 三浦芳樹, 美田敏宏.
  • Organizer: 生命科学系学会合同年次大会
• [Presentation] Ascaris suum 成虫およびCaenorhabditis elegans ミトコンドリアのプロテオーム解析: 二次元ゲル電気泳動法とショットガン質量分析法. (2017)
  • Author(s): 高宮信三郎, 数野彩子, 峯木礼子, 三浦芳樹, 美田敏宏
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] 宿主内競争による熱帯熱マラリア原虫のクロロキン感受性の回復. (2017)
  • Author(s): 橘真一郎, 池田美恵, Balikagala B, 櫻井美樹, 八代聖基, 関原誠, 山内祐人, Auma MA, 平井誠, 森稔幸, Palacpac N, 片岡正俊, 木村英作, 堀井俊宏, 美田敏宏.
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] Epidemiology of antimalarial drug resistance in Papua New Guinea. (2017)
  • Author(s): 関原誠, 橘真一郎, 美田敏宏.
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] Prerequisite Emergence of Background Mutations For Kelch13-Related Artemisinin-Resistant Plasmodium Falciparum Isolates. (2017)
  • Author(s): Tachibana S-I, Mori T, Hirai M, Mita T
  • Organizer: American Society of Tropical Medicine and Hygiene 66th Annual Meeting
  • Int'l Joint Research
• [Presentation] Development of See-Through Imaging Methods for Sexual Reproduction of Malaria Parasites. (2017)
  • Author(s): Mori T, Hirai M, Mita T
  • Organizer: American Society of Tropical Medicine and Hygiene 66th Annual Meeting
  • Int'l Joint Research
• [Presentation] Detection of artemisinin-resistant Plasmodium falciparum isolates with ex-vivo ring-stage survival assay in Uganda. (2017)
  • Author(s): Mita T, Ikeda M, Kaneko M, Balikagala B, Tachibana S-I, Sakurai-Yatsushiro M, Yatsushiro S, Takahashi N, Hashimoto M, Katuro O, Olia A, Obwoya P, Auma M, Anywar D, Odongo-Aginya E, Hirai M, Palacpac N, Kataoka M, Tsuboi T, Kimura E, Horii T.
  • Organizer: Keystone Symposia Conference: Malaria:
  • Int'l Joint Research
• [Presentation] A mutator Plasmodium berghei model to investigate antimalarial drug resistance. (2017)
  • Author(s): Mita T
  • Organizer: American Society of Tropical Medicine and Hygiene 66th Annual Meeting
  • Int'l Joint Research / Invited
• [Presentation] Pb102, A Novel Gene Essential for Female Fertility or Ookinete Maturation of Murine Malaria Parasite, Plasmodium Berghei. (2017)
  • Author(s): Maeta A, Hirai M, Mori T, Mita T
  • Organizer: American Society of Tropical Medicine and Hygiene 66th Annual Meeting
  • Int'l Joint Research
• [Presentation] Application of a cell microarray chip system for accurate, highly sensitive, and rapid diagnosis for malaria. (2017)
  • Author(s): Kataoka M, Yatsushiro S, Yamamoto T, Oka H, Okello-Onen J, Odongo-Aginya E, Palacpac N, Mita T, Baba Y, Horii T.
  • Organizer: Keystone Symposia Conference: Malaria
  • Int'l Joint Research
• [Presentation] Isolation of Piperaquine Resistant Rodent Malaria Parasite from Mutator Malaria. (2017)
  • Author(s): Hirai M, Ikeda M, Tachibana S-I, Mita T
  • Organizer: American Society of Tropical Medicine and Hygiene 66th Annual Meeting
  • Int'l Joint Research
• [Patent(Industrial Property Rights)] マラリア原虫の増殖抑制剤 (2019)
  • Inventor(s): 美田敏宏
  • Industrial Property Rights Holder: 美田敏宏
  • Industrial Property Rights Type: 特許
  • Filing Date: 2019
  • Acquisition Date: 2019
• [Patent(Industrial Property Rights)] マラリア原虫の増殖抑制剤 (2019)
  • Inventor(s): 美田敏宏、久保原禅
  • Industrial Property Rights Holder: 美田敏宏、久保原禅
  • Industrial Property Rights Type: 特許
  • Filing Date: 2019