| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2017: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Outline of Final Research Achievements |
In this study, the X-ray crystal structures of VepA and BEC, which are the major virulence factors of Vibrio parahaemolyticus and BEC-producing Clostridium perfringens, respectively, were analyzed to understand the mechanism of activity expression by the effector/toxin. For VepA, the structure of the complex with the chaperone VecA could be determined. Regarding BEC, X-ray crystal structures of apo-type and NADH complex were obtained for BECa having enzymatic activity, and the substrate recognition mechanism was elucidated at the atomic level. In addition, we were able to obtain new knowledge about the action of BECb on target cells.
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