Mechanistic analysis of hepatitis B virus entry and the host factors using new cell culture systems

• Project/Area Number: 17H04085
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Watashi Koichi 国立感染症研究所, 治療薬・ワクチン開発研究センター, 治療薬開発総括研究官 (40378948)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2019: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2017: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: HBV / 感染 / NTCP / EGFR / ウィルス

Outline of Final Research Achievements

Elucidation of the hepatitis B virus (HBV) cellular entry mechanisms is essential for understanding the tissue and species tropism of HBV infection and for developing new prevention and treatment against HBV. In this study, using highly HBV susceptible cell culture system, we identified a new cellular factor essential for HBV entry. We also revealed the mechanisms underlying the post-cell attachment of HBV and its application to drug development. Furthermore, we identified a molecular requirement for HBV cellular attachment and inhibitors of HBV attachment. This finding provides not only a virus-host interaction required for HBV infection but also a basis that determines the infection susceptibility of cells.

Academic Significance and Societal Importance of the Research Achievements

これまでHBVが侵入受容体NTCPを介して標的細胞表面へ吸着した後にどのように侵入するかはほとんど明らかでなかったが、本研究によって上皮成長因子受容体(EGFR)がHBV-NTCP複合体の細胞表面からのエンドサイトーシスに必須であることを明らかにした。またEGFRチロシンキナーゼ阻害薬であるゲフィチニブはHBV感染を抑制することが示された。このことは、HBV侵入が、NTCPを介した吸着およびEGFRを介した内在化と多段階に制御されていることを提唱するものであり、またEGFRが新たな抗HBV薬の標的となることも示したものである。

Research Products

• [Int'l Joint Research] INTS(フランス)
• [Int'l Joint Research] Pennsylvania State University(米国)
• [Int'l Joint Research] National Taiwan University(台湾)
• [Int'l Joint Research] INTS(フランス)
• [Int'l Joint Research] インディアナ大学/ロスアラモス国立研究所(米国)
• [Int'l Joint Research] ヴィットウォーターズラント大学(南アフリカ)
• [Journal Article] Surfeit 4 contributes to the replication of hepatitis C virus using double membrane vesicles. (2020)
  • Author(s): Kong L, Aoyagi H, Yang Z, Ouyang T, Matsuda M, Fujimoto A, Watashi K, Suzuki R, Arita M, Yamagoe S, Dohmae N, Suzuki T, Suzuki T, Muramatsu M, Wakita T, Aizaki H.
  • Journal Title: Journal of Virology Volume: 94 Issue: 2
  • DOI: 10.1128/jvi.00858-19
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Anti‐viral effects of interferon‐λ3 on hepatitis B virus infection in cell culture (2020)
  • Author(s): Yamada Norie、Murayama Asako、Shiina Masaaki、Aly Hussein Hassan、Iwamoto Masashi、Tsukuda Senko、Watashi Koichi、Tanaka Tomohisa、Moriishi Kohji、Nishitsuji Hironori、Sugiyama Masaya、Mizokami Masashi、Shimotohno Kunitada、Muramatsu Masamichi、Murata Kazumoto、Kato Takanobu
  • Journal Title: Hepatology Research Volume: 50 Issue: 3 Pages: 283-291
  • DOI: 10.1111/hepr.13449
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The machinery for endocytosis of epidermal growth factor receptor coordinates the transport of incoming hepatitis B virus to the endosomal network. (2020)
  • Author(s): Iwamoto M, Saso W, Nishioka K, Ohashi H, Sugiyama R, Ryo A, Ohki M, Yun JH, Park SY, Ohshima T, Suzuki R, Aizaki H, Muramatsu M, Matano T, Iwami S, Sureau C, Wakita T, Watashi K
  • Journal Title: J Biol Chem Volume: 295 Issue: 3 Pages: 800-807
  • DOI: 10.1074/jbc.ac119.010366
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Prolyl Isomerase Pin1 Regulates the Stability of Hepatitis B Virus Core Protein. (2020)
  • Author(s): Nishi M, Miyakawa K, Matsunaga S, Khatun H, Yamaoka Y, Watashi K, Sugiyama M, Kimura H, Wakita T, Ryo A.
  • Journal Title: Frontiers in Cell and Developmental Biology Volume: 8 Pages: 26-26
  • DOI: 10.3389/fcell.2020.00026
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Engineering Cellular Biosensors with Customizable Antiviral Responses Targeting Hepatitis B Virus (2020)
  • Author(s): Matsunaga Satoko、Jeremiah Sundararaj S.、Miyakawa Kei、Kurotaki Daisuke、Shizukuishi Sayaka、Watashi Koichi、Nishitsuji Hironori、Kimura Hirokazu、Tamura Tomohiko、Yamamoto Naoki、Shimotohno Kunitada、Wakita Takaji、Ryo Akihide
  • Journal Title: iScience Volume: 23 Issue: 3 Pages: 100867-100867
  • DOI: 10.1016/j.isci.2020.100867
  • Peer Reviewed / Open Access
• [Journal Article] The Beginning of Ending Hepatitis C Virus: A Summary of the 26th International Symposium on Hepatitis C Virus and Related Viruses (2020)
  • Author(s): Shin Eui-Cheol、Han Ji Won、Kang Wonseok、Kato Takanobu、Kim Seong-Jun、Zhong Jin、Kim Seungtaek、Park Su-Hyung、Sung Pil Soo、Watashi Koichi、Park Jun Yong、Windisch Marc P.、Oh Jong-Won、Wakita Takaji、Han Kwang-Hyub、Jang Sung Key
  • Journal Title: Viruses Volume: 12 Issue: 3 Pages: 302-302
  • DOI: 10.3390/v12030302
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] N‐terminal PreS1 Sequence Regulates Efficient Infection of Cell Culture‐generated Hepatitis B Virus (2020)
  • Author(s): Murayama A、Yamada N、Osaki 、Shiina 、Aly H H、Iwamoto M、Tsukuda S、Watashi K、Matsuda M、Suzuki R、Tanaka T、Moriishi K、Suzuki T、Nishitsuji H、Sugiyama M、Mizokami M、Shimotohno K、Wakita T、Muramatsu M、Liang T J、Kato T
  • Journal Title: Hepatology Volume: 「－」 Issue: 2 Pages: 520-532
  • DOI: 10.1002/hep.31308
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] From the Establishment of Hepatitis B Virus Cell Culture Systems to Drug Discovery (2019)
  • Author(s): 深野顕人、渡士幸一
  • Journal Title: YAKUGAKU ZASSHI Volume: 139 Issue: 1 Pages: 81-87
  • DOI: 10.1248/yakushi.18-00164-4
  • NAID: 130007541645
  • ISSN: 0031-6903, 1347-5231
  • Year and Date: 2019-01-01
  • Peer Reviewed / Open Access
• [Journal Article] A global scientific strategy to cure hepatitis B (2019)
  • Author(s): Revill PA, Chisari FV, Block JM, Dandri M, Gehring AJ, Guo H, Hu J, Kramvis A, Lampertico P, Janssen HLA, Levrero M, Li W, Liang TJ, Lim SG, Lu F, Penicaud MC, Tavis JE, Thimme R, Arbuthnot P, Boonstra PA, Chang KM, Chen PJ, Glebe D, Guidotti LG, Fellay J, Ferrari C, Jansen L, Lau DTY, Lok AS, Maini MK, Mason W, et al.
  • Journal Title: Lancet Gastroenterol Hepatol Volume: 4 Issue: 7 Pages: 545-558
  • DOI: 10.1016/s2468-1253(19)30119-0
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Retrotransposon elements among initial sites of hepatitis B virus integration into human genome in the HepG2-NTCP cell infection model (2019)
  • Author(s): Chauhan R, Shimizu Y, Watashi K, Wakita T, Fukasawa M, Michalak TI
  • Journal Title: Cancer Genet Volume: 235-236 Pages: 39-56
  • DOI: 10.1016/j.cancergen.2019.04.060
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Small Interfering RNA Screening for the Small GTPase Rab Proteins Identifies Rab5B as a Major Regulator of Hepatitis B Virus Production (2019)
  • Author(s): Inoue Jun、Ninomiya Masashi、Umetsu Teruyuki、Nakamura Takuya、Kogure Takayuki、Kakazu Eiji、Iwata Tomoaki、Takai Satoshi、Sano Akitoshi、Fukuda Mitsunori、Watashi Koichi、Isogawa Masanori、Tanaka Yasuhito、Shimosegawa Tooru、McNiven Mark A.、Masamune Atsushi
  • Journal Title: Journal of Virology Volume: 93 Issue: 15
  • DOI: 10.1128/jvi.00621-19
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] An interferon-like small chemical compound CDM-3008 suppresses hepatitis B virus through induction of interferon-stimulated genes (2019)
  • Author(s): Furutani Yutaka、Toguchi Mariko、Shiozaki-Sato Yumi、Qin Xian-Yang、Ebisui Etsuko、Higuchi Shoko、Sudoh Masayuki、Suzuki Harukazu、Takahashi Nobuaki、Watashi Koichi、Wakita Takaji、Kakeya Hideaki、Kojima Soichi
  • Journal Title: PLOS ONE Volume: 14 Issue: 6 Pages: e0216139-e0216139
  • DOI: 10.1371/journal.pone.0216139
  • NAID: 120006644069
  • Peer Reviewed / Open Access
• [Journal Article] Bardoxolone methyl as a novel potent antiviral agent against hepatitis B and C viruses in human hepatocyte cell culture systems. (2019)
  • Author(s): Nio Yasunori、Sasai Machiko、Akahori Yuichi、Okamura Hitomi、Hasegawa Hikari、Oshima Mizuki、Watashi Koichi、Wakita Takaji、Ryo Akihide、Tanaka Yasuhito、Hijikata Makoto
  • Journal Title: Antiviral Research Volume: 169 Pages: 104537-104537
  • DOI: 10.1016/j.antiviral.2019.104537
  • Peer Reviewed
• [Journal Article] Pyrimidotriazine derivatives as selective inhibitors of HBV capsid assembly (2019)
  • Author(s): Toyama M, Sakakibara N, Takeda M, Okamoto M, Watashi K, Wakita T, Sugiyama M, Mizokami M, Ikeda M, Baba M
  • Journal Title: Virus Res Volume: 271 Pages: 197677-197677
  • DOI: 10.1016/j.virusres.2019.197677
  • Peer Reviewed
• [Journal Article] Development of an in vitro cholestatic drug-induced liver injury evaluation system using HepG2-hNTCP-C4 cells in sandwich configuration (2019)
  • Author(s): Sakai Yoko、Okumura Hiroki、Iwao Takahiro、Watashi Koichi、Ito Kousei、Matsunaga Tamihide
  • Journal Title: Toxicology in Vitro Volume: 61 Pages: 104619-104619
  • DOI: 10.1016/j.tiv.2019.104619
  • Peer Reviewed
• [Journal Article] Immunomodulatory mechanism of acyclic nucleoside phosphates in treatment of hepatitis B virus infection. (2019)
  • Author(s): Murata K, Tsukuda S, Suizu F, Kimura A, Sugiyama M, Watashi K, Noguchi M, Mizokami M.
  • Journal Title: Hepatology Volume: 71 Issue: 5 Pages: 1533-1545
  • DOI: 10.1002/hep.30956
  • Peer Reviewed
• [Journal Article] Synthesis of nucleotide analogues, EFdA, EdA and EdAP, and the effect of EdAP on hepatitis B virus replication (2019)
  • Author(s): Kamata Mai、Takeuchi Toshifumi、Hayashi Ei、Nishioka Kazane、Oshima Mizuki、Iwamoto Masashi、Nishiuchi Kota、Kamo Shogo、Tomoshige Shusuke、Watashi Koichi、Kamisuki Shinji、Ohrui Hiroshi、Sugawara Fumio、Kuramochi Kouji
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 84 Issue: 2 Pages: 217-227
  • DOI: 10.1080/09168451.2019.1673696
  • Peer Reviewed
• [Journal Article] Non-nucleoside hepatitis B virus polymerase inhibitors identified by an in vitro polymerase elongation assay (2019)
  • Author(s): Nakajima Shogo、Watashi Koichi、Fukano Kento、Tsukuda Senko、Wakae Kousho、Aizaki Hideki、Muramatsu Masamichi、Wakita Takaji、Toyoda Tetsuya
  • Journal Title: Journal of Gastroenterology Volume: 55 Issue: 4 Pages: 441-452
  • DOI: 10.1007/s00535-019-01643-0
  • Peer Reviewed
• [Journal Article] Epidermal growth factor receptor is a host entry cofactor triggering hepatitis B virus internalization (2019)
  • Author(s): M. Iwamoto, W. Saso, R. Sugiyama, K. Ishii, M. Ohki, S. Nagamori, R. Suzuki, H. Aizaki, A. Ryo, J-H Yun, S-Y Park, N. Ohtani, M. Muramatsu, S. Iwami, Y. Tanaka, C. Sureau, T. Wakita and K. Watashi
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 116 Issue: 17 Pages: 8487-8492
  • DOI: 10.1073/pnas.1811064116
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Concept of Viral Inhibitors via NTCP. (2019)
  • Author(s): 1)Tsukuda S, Fukano K, Watashi K, Wakita T.
  • Journal Title: Semin Liver Dis. Volume: 39 Issue: 01 Pages: 78-95
  • DOI: 10.1055/s-0038-1676804
  • Peer Reviewed
• [Journal Article] Screening for inhibitor of episomal DNA identified dicumarol as a hepatitis B virus inhibitor (2019)
  • Author(s): Takeuchi F, Ikeda S, Tsukamoto Y, Iwasawa Y, Qihao C, Otakaki Y, Ryota O, Yao WL, Narita R, Makoto H, Watashi K, Wakita T, Takeuchi K, Chayama K, Kogure A, Kato H, Fujita T
  • Journal Title: PLoS One Volume: 14 Issue: 2 Pages: e0212233-e0212233
  • DOI: 10.1371/journal.pone.0212233
  • NAID: 120006822498
  • Peer Reviewed / Open Access
• [Journal Article] Activation of protein kinase R by hepatitis C virus RNA-dependent RNA polymerase. (2019)
  • Author(s): Suzuki R, Matsuda M, Shimoike T, Watashi K, Aizaki H, Kato T, Suzuki T, Muramatsu M, Wakita T.
  • Journal Title: Virology Volume: 529 Pages: 226-233
  • DOI: 10.1016/j.virol.2019.01.024
  • Peer Reviewed
• [Journal Article] Troglitazone Impedes the Oligomerization of Sodium Taurocholate Cotransporting Polypeptide and Entry of Hepatitis B Virus Into Hepatocytes (2019)
  • Author(s): Fukano Kento、Tsukuda Senko、Oshima Mizuki、Suzuki Ryosuke、Aizaki Hideki、Ohki Mio、Park Sam-Yong、Muramatsu Masamichi、Wakita Takaji、Sureau Camille、Ogasawara Yuki、Watashi Koichi
  • Journal Title: Frontiers in Microbiology Volume: 9 Pages: 3257-3257
  • DOI: 10.3389/fmicb.2018.03257
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mathematical analysis of a transformed ODE from a PDE multiscale model of hepatitis C virus infection (2019)
  • Author(s): Kitagawa K., Kuniya T., Nakaoka S., Asai Y., Watashi K. and Iwami S.
  • Journal Title: Bulletin of Mathematical Biology Volume: 81(5) Issue: 5 Pages: 1427-1441
  • DOI: 10.1007/s11538-018-00564-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Peroxiredoxin 1, a novel HBx-interacting protein, interacts with exsome component 5 and negatively regulates hepatitis B virus (HBV) propagation through degradation of HBV RNA. (2019)
  • Author(s): Lin Deng, Xiang Gan, Masahiko Ito, Ming Chen, Hussein H. Aly, Chieko Matsui, Takayuki Abe, Koichi Watashi,Takaji Wakita, Tetsuro Suzuki, Toru Okamoto, Yoshiharu Matsuura, Masashi Mizokami, Ikuo Shoji, and Hak Hotta.
  • Journal Title: J. Virol Volume: 93 Issue: 6
  • DOI: 10.1128/jvi.02203-18
  • Peer Reviewed / Open Access
• [Journal Article] Acidic polysaccharides isolated from marine algae inhibit the early step of viral infection (2019)
  • Author(s): Ueno Mikinori、Nogawa Masato、Siddiqui Rokeya、Watashi Koichi、Wakita Takaji、Kato Nobuyuki、Ikeda Masanori、Okimura Takasi、Isaka Shogo、Oda Tatsuya、Ariumi Yasuo
  • Journal Title: International Journal of Biological Macromolecules Volume: 124 Pages: 282-290
  • DOI: 10.1016/j.ijbiomac.2018.11.152
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Single Adaptive Mutation in Sodium Taurocholate Cotransporting Polypeptide Induced by Hepadnaviruses Determines Virus Species Specificity (2018)
  • Author(s): Takeuchi Junko S.、Fukano Kento、Iwamoto Masashi、Tsukuda Senko、Suzuki Ryosuke、Aizaki Hideki、Muramatsu Masamichi、Wakita Takaji、Sureau Camille、Watashi Koichi
  • Journal Title: Journal of Virology Volume: 93 Issue: 5 Pages: 04132-18
  • DOI: 10.1128/jvi.01432-18
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] High-throughput neutralization assay for multiple flaviviruses based on single-round infectious particles using dengue virus type 1 reporter replicon. (2018)
  • Author(s): Matsuda M, Yamanaka A, Yato K, Yoshii K, Watashi K, Aizaki H, Konishi E, Takasaki T, Kato T, Muramatsu M, Wakita T, Suzuki R
  • Journal Title: Sci Rep Volume: 8 Issue: 1 Pages: 16624-16624
  • DOI: 10.1038/s41598-018-34865-y
  • Peer Reviewed / Open Access
• [Journal Article] The aryl hydrocarbon receptor?cytochrome P450 1A1 pathway controls lipid accumulation and enhances the permissiveness for hepatitis C virus assembly (2018)
  • Author(s): Ohashi Hirofumi、Nishioka Kazane、Nakajima Syo、Kim Sulyi、Suzuki Ryosuke、Aizaki Hideki、Fukasawa Masayoshi、Kamisuki Shinji、Sugawara Fumio、Ohtani Naoko、Muramatsu Masamichi、Wakita Takaji、Watashi Koichi
  • Journal Title: Journal of Biological Chemistry Volume: 293 Issue: 51 Pages: 19559-19571
  • DOI: 10.1074/jbc.ra118.005033
  • Peer Reviewed
• [Journal Article] Cell and Animal Models for Studying Hepatitis B Virus Infection and Drug Development (2018)
  • Author(s): Hu J, Lin YY, Chen PJ, Watashi K, Wakita T
  • Journal Title: Gastroenterology Volume: 156 Issue: 2 Pages: 338-354
  • DOI: 10.1053/j.gastro.2018.06.093
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] IL-1β/ATF3-mediated induction of Ski2 expression enhances hepatitis B virus x mRNA degradation (2018)
  • Author(s): Shiromoto Fumihiro、Aly Hussein H.、Kudo Haruka、Watashi Koichi、Murayama Asako、Watanabe Noriyuki、Zheng Xin、Kato Takanobu、Chayama Kazuaki、Muramatsu Masamichi、Wakita Takaji
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 503 Issue: 3 Pages: 1854-1860
  • DOI: 10.1016/j.bbrc.2018.07.126
  • Peer Reviewed
• [Journal Article] Screening of microRNAs for a repressor of hepatitis B virus replication (2018)
  • Author(s): Naito Y, Hamada-Tsutsumi S, Yamamoto Y, Kogure A, Yoshioka Y, Watashi K, Ochiya T, Tanaka Y
  • Journal Title: Oncotarget Volume: 9 Issue: 52 Pages: 29857-29868
  • DOI: 10.18632/oncotarget.25557
  • Peer Reviewed / Open Access
• [Journal Article] Flap endonuclease 1 is involved in cccDNA formation in the hepatitis B virus (2018)
  • Author(s): Kitamura Kouichi、Que Lusheng、Shimadu Miyuki、Koura Miki、Ishihara Yuuki、Wakae Kousho、Nakamura Takashi、Watashi Koichi、Wakita Takaji、Muramatsu Masamichi
  • Journal Title: PLOS Pathogens Volume: 14 Issue: 6 Pages: e1007124-e1007124
  • DOI: 10.1371/journal.ppat.1007124
  • NAID: 120006602144
  • Peer Reviewed / Open Access
• [Journal Article] Development of a cell-based assay to identify hepatitis B virus entry inhibitors targeting the sodium taurocholate cotransporting polypeptide (2018)
  • Author(s): Miyakawa Kei、Matsunaga Satoko、Yamaoka Yutaro、Dairaku Mina、Fukano Kento、Kimura Hirokazu、Chimuro Tomoyuki、Nishitsuji Hironori、Watashi Koichi、Shimotohno Kunitada、Wakita Takaji、Ryo Akihide
  • Journal Title: Oncotarget Volume: 9 Issue: 34 Pages: 23681-23694
  • DOI: 10.18632/oncotarget.25348
  • Peer Reviewed / Open Access
• [Journal Article] Rosmarinic acid is a novel inhibitor for Hepatitis B virus replication targeting viral epsilon RNA-polymerase interaction (2018)
  • Author(s): Tsukamoto Yuta、Ikeda Sotaro、Uwai Koji、Taguchi Riho、Chayama Kazuaki、Sakaguchi Takemasa、Narita Ryo、Yao Wan-Ling、Takeuchi Fumihiko、Otakaki Yukie、Watashi Koichi、Wakita Takaji、Kato Hiroki、Fujita Takashi
  • Journal Title: PLOS ONE Volume: 13 Issue: 5 Pages: e0197664-e0197664
  • DOI: 10.1371/journal.pone.0197664
  • NAID: 120006649572
  • Peer Reviewed / Open Access
• [Journal Article] De Novo Macrocyclic Peptide Inhibitors of Hepatitis B Virus Cellular Entry. (2018)
  • Author(s): Passioura T, Watashi K, Fukano K, Shimura S, Saso W, Morishita R, Ogasawara Y, Tanaka Y, Mizokami M, Sureau C, Suga H, Wakita T.
  • Journal Title: Cell Chem Biol. Volume: 25 Issue: 7 Pages: 906-915
  • DOI: 10.1016/j.chembiol.2018.04.011
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A new strategy to identify hepatitis B virus entry inhibitors by AlphaScreen technology targeting the envelope-receptor interaction. (2018)
  • Author(s): Saso W, Tsukuda S, Ohashi H, Fukano K, Morishita R, Matsunaga S, Ohki M, Ryo A, Park SY, Suzuki R, Aizaki H, Muramatsu M, Sureau C, Wakita T, Matano T, Watashi K.
  • Journal Title: Biochem Biophys Res Commun Volume: 印刷中 Issue: 2 Pages: 374-379
  • DOI: 10.1016/j.bbrc.2018.04.187
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Inhibitory effect of fasiglifam on hepatitis B virus infections through suppression of the sodium taurocholate cotransporting polypeptide (2018)
  • Author(s): Nio Y, Akahori Y, Okamura H, Watashi K, Wakita T, Hijikata M
  • Journal Title: Biochem Biophys Res Commun Volume: 501 Issue: 3 Pages: 820-825
  • DOI: 10.1016/j.bbrc.2018.04.199
  • Peer Reviewed
• [Journal Article] A PDE multiscale model of hepatitis C virus infection can be transformed to a system of ODEs (2018)
  • Author(s): K. Kitagawa, S. Nakaoka, Y. Asai, K. Watashi, S. Iwami
  • Journal Title: Journal of Theoretical Biology Volume: 48 Pages: 80-85
  • DOI: 10.1016/j.jtbi.2018.04.006
  • Peer Reviewed / Open Access
• [Journal Article] Novel stable HBV producing cell line systems for expression and screening antiviral inhibitor of hepatitis B virus in human hepatoma cell line (2018)
  • Author(s): Ogura N, Ogawa K, Watashi K, Ito T, Wakita T
  • Journal Title: Biochem Biophys Res Commun Volume: 498 Issue: 1 Pages: 64-71
  • DOI: 10.1016/j.bbrc.2018.02.175
  • Peer Reviewed
• [Journal Article] Chemical array system, a platform to identify novel hepatitis B virus entry inhibitors targeting sodium taurocholate cotransporting polypeptide (2018)
  • Author(s): Kaneko M, Futamura Y, Tsukuda S, Kondoh Y, Sekine T, Hirano H, Fukano K, Ohashi H, Saso W, Morishita R, Matsunaga S, Kawai F, Ryo A, Park S-Y, Suzuki R, Aizaki H, Ohtani N, Sureau C, Wakita T, Osada H, Watashi K
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 2769-2769
  • DOI: 10.1038/s41598-018-20987-w
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Revealing the characteristics of antiviral agents (2017)
  • Author(s): 大橋啓史、渡士幸一
  • Journal Title: Uirusu Volume: 67 Issue: 2 Pages: 133-142
  • DOI: 10.2222/jsv.67.133
  • ISSN: 0042-6857, 1884-3433
  • Year and Date: 2017-12-25
• [Journal Article] Involvement of PUF60 in Transcriptional and Post-transcriptional Regulation of Hepatitis B Virus Pregenomic RNA Expression (2017)
  • Author(s): Sun Suofeng、Nakashima Kenji、Ito Masahiko、Li Yuan、Chida Takeshi、Takahashi Hirotaka、Watashi Koichi、Sawasaki Tatsuya、Wakita Takaji、Suzuki Tetsuro
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 12874-12874
  • DOI: 10.1038/s41598-017-12497-y
  • Peer Reviewed / Open Access
• [Journal Article] Reply to Padmanabhan and Dixit: Hepatitis C virus entry inhibitors for optimally boosting direct-acting antiviral-based treatments. (2017)
  • Author(s): Ohashi HK, Koizumi Y, Fukano K, Wakita T, Perelson AS, Iwami S, Watashi K
  • Journal Title: Proc Acad Natl Acad USA Volume: 114 Issue: 23
  • DOI: 10.1073/pnas.1705234114
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Functional association of cellular microtubules with viral capsid assembly supports efficient hepatitis B virus replication (2017)
  • Author(s): Iwamoto M、Cai D、Sugiyama M、Suzuki R、Aizaki H、Ryo A、Ohtani N、Tanaka Y、Mizokami M、Wakita T、Guo H、Watashi K
  • Journal Title: Sci Rep Volume: 7 Issue: 1 Pages: 10620-10620
  • DOI: 10.1038/s41598-017-11015-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Hepatitis B virus prevents excessive viral production via reduction of cell death-inducing DFF45-like effectors (2017)
  • Author(s): Yasumoto Jun、Kasai Hirotake、Yoshimura Kentaro、Otoguro Teruhime、Watashi Koichi、Wakita Takaji、Yamashita Atsuya、Tanaka Tomohisa、Takeda Sen、Moriishi Kohji
  • Journal Title: Journal of General Virology Volume: 98 Issue: 7 Pages: 1762-1773
  • DOI: 10.1099/jgv.0.000813
  • Peer Reviewed
• [Journal Article] ssAAVs containing cassettes encoding SaCas9 and guides targeting hepatitis B virus inactivate replication of the virus in cultured cells (2017)
  • Author(s): Scott T, Moyo B, Nicholson S, Maepa MB, Watashi K, Ely A, Weinberg MS, Arbuthnot P
  • Journal Title: Sci Rep Volume: 7 Issue: 1 Pages: 7401-7401
  • DOI: 10.1038/s41598-017-07642-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 目覚ましいC型肝炎治療の進歩 (2017)
  • Author(s): 渡士幸一
  • Journal Title: 感染・炎症・免疫 Volume: 47 Pages: 71-72
• [Journal Article] 抗B型肝炎ウイルス薬の現状と新規薬剤開発のアプローチ (2017)
  • Author(s): 九十田千子、渡士幸一
  • Journal Title: 化学療法の領域 Volume: 33 Pages: 73-78
• [Presentation] B型肝炎ウイルス培養系と 薬剤開発への応用 (2019)
  • Author(s): 渡士 幸一
  • Organizer: 京都大学ウイルス・再生医科学研究所 再生医学・再生医療の先端融合的共同研究拠点 ウイルス感染症・生命科学先端融合的共同研究拠点 平成30年度共同研究合同報告会
  • Invited
• [Presentation] NTCP/SLC10A1 の胆汁酸取り込み機能に影響しない選択的B型肝炎ウイルス侵入阻害ペプチドの同定 (2019)
  • Author(s): 深野 顕人, Toby PASSIOURA, 志村 聡美, 佐宗 若奈, 森下 了, 小笠原 裕樹, 田中 靖人, 溝上 雅史, Camille SUREAU, 脇田 隆字, 菅 裕明, 渡士 幸一
  • Organizer: 日本薬学会第139年会
• [Presentation] Interaction of host and hepatitis viruses as a target for drug development (2018)
  • Author(s): Koichi Watashi
  • Organizer: Taiwan Society of Virology, Symposium in Memory of Dr. Kuan-Teh Jeang: Human Retrovirus and Hepatitis virus
  • Int'l Joint Research / Invited
• [Presentation] Clinical and Virological Significance of NTCP Receptor in HBV (2018)
  • Author(s): Koichi Watashi
  • Organizer: 2018 APASL Single Topic Conference on Hepatitis B Virus (2018 APASL STC Taipei)
  • Int'l Joint Research / Invited
• [Presentation] Cell entry inhibition as an antiviral strategy (2018)
  • Author(s): Koichi Watashi
  • Organizer: 2018 APASL Single Topic Conference on Hepatitis B Virus (2018 APASL STC Taipei)
  • Int'l Joint Research / Invited
• [Presentation] 核内受容体を利用した肝炎ウイルス増殖戦略 (2018)
  • Author(s): 渡士 幸一
  • Organizer: 日本大学医学部セミナー
  • Invited
• [Presentation] HBVエントリー阻害剤Tomatineの同定と抗HBV活性の解析 (2018)
  • Author(s): 古谷裕, 戸口真理子, 佐藤裕美, 九十田千子, 渡士幸一, 湯川博, 石川哲也, 黒田俊一, 上田啓次, 田中靖人, 脇田隆字, 小嶋聡一
  • Organizer: 第28回抗ウイルス療法学会学術集会・総会
• [Presentation] スフィンゴ脂質のC型肝炎ウイルス複製における役割の解析 (2018)
  • Author(s): 相崎英樹, ホッサムゲワイド, 青柳東代, Zheng Xin, Fransec Puig-Basagoiti, 渡士幸一, 鈴木亮介, 熊谷圭悟, 山地俊之, 深澤征義, 酒巻有里子, 花田賢太郎, 村松正道, 脇田隆字
  • Organizer: 第28回抗ウイルス療法学会学術集会・総会
• [Presentation] 培養細胞系を利用した HBV cccDNA ダイナミクスの定量的検討 (2018)
  • Author(s): 渡士 幸一, 九十田 千子, 脇田 隆字
  • Organizer: 第54回日本肝臓学会総会
• [Presentation] B型肝炎ウイルスの創薬研究 (2018)
  • Author(s): 脇田 隆字, 渡士 幸一
  • Organizer: 第54回日本肝臓学会総会
• [Presentation] Aryl hydrocarbon receptor-cytochrome p450 1a1 pathway regulates hepatic lipid biosynthesis to maximize Hepatitis C virus production (2018)
  • Author(s): Ohashi H, Nakajima S, Kim S, Suzuki R, Aizaki H, Fukasawa M, Kamisuki S, Sugawara F, Ohtani N, Muramatsu M, Wakita T, Watashi K
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] Hepatitis C virus infection triggers the transactivation of aryl hydrocarbon receptor to rearrange hepatic lipid biosynthesis (2018)
  • Author(s): Ohashi H, Nakajima S, Kim S, Suzuki R, Aizaki H, Fukasawa M, Kamisuki S, Sugawara F, Ohtani N, Muramatsu M, Wakita T, Watashi K
  • Organizer: The 66th Annual Meeting of the Japanese Society for Virology
• [Presentation] Oligomerization of NTCP is required for hepatitis B virus internalization (2018)
  • Author(s): Fukano K, Tsukuda S, Ohki M, Park S, Suzuki R, Aizaki H, Muramatsu M, Wakita T, Ogasawara Y, Watashi K
  • Organizer: The 66th Annual Meeting of the Japanese Society for Virology
• [Presentation] AlphaScreen technology targeting the viral envelope-receptor interaction identified a novel HBV entry inhibitor, rapamycin (2018)
  • Author(s): Saso W, Tsukuda S, Ohashi H, Fukano K, Morishita R, Matsunaga S, Ohki M, Ryo A, Park S, Suzuki R, Aizaki H, Muramatsu M, Wakita T, Matano T, Watashi K
  • Organizer: The 66th Annual Meeting of the Japanese Society for Virology
• [Presentation] Generation of monocloneal antibodies against hepatitis B virus preS1 region from antigen-specific memory B cells (2018)
  • Author(s): Yato K, Onodera T, Matsuda M, Fujimoto A, Watashi K, Aizaki H, Kato T, Moriishi K, Tamura K, Takahashi Y, Wakita T, Muramatsu M, Suzuki R
  • Organizer: The 66th Annual Meeting of the Japanese Society for Virology
• [Presentation] Establishment of a model for the quantification of hepatitis B virus cccDNA dynamics (2018)
  • Author(s): Kakizoe Y, Iwamoto M, Davenport MP, Watashi K, Iwami S
  • Organizer: The 66th Annual Meeting of the Japanese Society for Virology
• [Presentation] A comparison between HCV JFH-1 and Jc1 strains by quantitative analysis of infection dynamics (2018)
  • Author(s): Iwanani S, Ohashi H, Asai Y, Watashi K, Iwami S
  • Organizer: The 66th Annual Meeting of the Japanese Society for Virology
• [Presentation] HCV genome and abnomal oraganelles in the liver after elimination of hepatitis C virus by drug treatment (2018)
  • Author(s): Aizaki H, Aoyagi H, Iijima H, Zheng X, Matsuda M, Watashi K, Suzuki R, Masaki T, Shimada N, Kato K, Tsubota A, Saito T, Hayashi K, Enomoto M, Mimata A, Sakamaki Y, Ichinose S, Wakae K, Muramatsu M, Wakita T
  • Organizer: The 66th Annual Meeting of the Japanese Society for Virology
• [Presentation] Identification of aryl hydrocarbon receptor ligands inhibiting the lipid accumulation and hepatitis C virus production (2018)
  • Author(s): Nishioka K, Ohashi H, Suzuki R, Aizaki H, Muramatsu M, Wakita T, Watashi K
  • Organizer: The 66th Annual Meeting of the Japanese Society for Virology
• [Presentation] Drug screening for suppression of lipogenesis through downregulation of C/EBPa (2018)
  • Author(s): Satoh S, Onomura D, Watashi K, Ueda Y, Dansako H, Honda M, Kaneko S, Kato N
  • Organizer: The 66th Annual Meeting of the Japanese Society for Virology
• [Presentation] Bardoxolon methyl suppresses the prolifieration of both hepatitis B and C viruses in culture cells (2018)
  • Author(s): Akahori Y, Okamura H, Sasaki M, Hasegawa H, Watashi K, Wakita T, Tanaka Y, Nio Y, Hijikata M
  • Organizer: The 66th Annual Meeting of the Japanese Society for Virology
• [Presentation] Mechanistical study of antiviral activity of snake venom sPLA2 against HBV and HCV (2018)
  • Author(s): Miyayama Y, Chen M, Aoki-Utsubo C, Deng L, Shindo K, Noda T, Kohara M, Watashi K, Wakita T, Hijikata M, Hotta H
  • Organizer: The 66th Annual Meeting of the Japanese Society for Virology
• [Presentation] Identification of novel HBV entry inhibitors by in vitro technologies (2018)
  • Author(s): Koichi Watashi
  • Organizer: 6th Taiwan-Japan-Korea HBV Research Symposium 2018
  • Int'l Joint Research
• [Presentation] B型肝炎ウイルス侵入阻害ペプチドの同定とその機序の解析 (2018)
  • Author(s): 深野顕人、Passioura Toby、志村聡美、佐宗若奈、森下了、小笠原裕樹、田中靖人、溝上雅史、Sureau Camille、菅裕明、脇田隆字、渡士幸一
  • Organizer: 第15回ウイルス学キャンプin湯河原
• [Presentation] B型肝炎ウイルス侵入に関わる宿主因子の同定と作用機序解析 (2018)
  • Author(s): Wakana Saso, Masashi Iwamoto, Masamichi Muramatsu, Tetsuro Matano, Koichi Watashi
  • Organizer: 第15回ウイルス学キャンプin湯河原
• [Presentation] ウイルス-脂質相互作用に関わる核内受容体の解析 (2018)
  • Author(s): Kazane Nishioka, Hirofumi Ohashi, Ryosuke Suzuki, Hideki Aizaki, Masamichi Muramatsu, Takaji Wakita, Koichi Watashi
  • Organizer: 第15回ウイルス学キャンプin湯河原
• [Presentation] D型肝炎ウイルス生存戦略の理解と意義について (2018)
  • Author(s): 岩本将士, 岩見真吾, 渡士幸一
  • Organizer: 第15回ウイルス学キャンプin湯河原
• [Presentation] Oligomerization of NTCP induces hepatitis B virus internalization (2018)
  • Author(s): Kento Fukano, Senko Tsukuda, Mio Ohki, Sam-Yong Park, Ryosuke Suzuki, Hideki Aizaki, Masamichi Muramatsu, Takaji Wakita, Yuki Ogasawara, Koichi Watashi
  • Organizer: 2018 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] AlphaScreen assay targeting the NTCP-large surface antigen interaction identified rapamycin and its derivatives as novel hepatitis B virus entry inhibitors (2018)
  • Author(s): Wakana Saso, Senko Tsukuda, Hirofumi Ohashi, Kento Fukano, Ryo Morishita, Satoko Matsunaga, Mio Ohki, Akihide Ryo, Sam-Yong Park, Ryosuke Suzuki, Hideki Aizaki, Masamichi Muramatsu, Camille Sureau, Takaji Wakita, Tetsuro Matano, Koichi Watashi
  • Organizer: 2018 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] Ultrastructure of hepatocytes in chronic hepatitis B patients (2018)
  • Author(s): Haruyo Aoyagi, Hiroki Iijima, Xin Zheng, Yu Ting Kao, Koichi Watashi, Ryosuke Suzuki, Noritomo Shimada, Keizo Kato, Akihito Tsubota, Ayako Mimata, Yuriko Sakamaki, Shizuko Ichinose, Kenjiro Wake, Masamichi Muramatsu, Takaji Wakita, Hideki Aizaki
  • Organizer: 2018 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] Retrotransposon elements are among initial sites of HBV integration into human genome in HBV-HepG2-NTCP cell infection model (2018)
  • Author(s): Thomas Michalak, Ranjit Chauhan, Yoshimi Shimizu, Koichi Watashi, Takaji Wakita, Masayoshi Fukasawa
  • Organizer: 2018 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] RNA granule components regulate HBV replication (2018)
  • Author(s): Yasuo Ariumi, Koichi Watashi, Mikinori Ueno, Lin Deng, Hak Hotta, Takaji Wakita
  • Organizer: 2018 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] Rosmarinic acid is a novel inhibitor for Hepatitis B virus replication targeting viral epsilon RNA-polymerase binding (2018)
  • Author(s): Yuta Tsukamoto, Sotaro Ikeda, Koji Uwai, Riho Taguchi, Kazuaki Chayama, Takemasa Sakaguchi, Ryo Narita, Wan-Ling Yao, Fumihiko Takeuchi, Yukie Otakaki, Koichi Watashi, Takaji Wakita, Hiroki Kato, Takashi Fujita
  • Organizer: 2018 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] Engineering of HBV-specific SynNotch receptor to estabilsh biosensor cells (2018)
  • Author(s): Satoko Matsunaga, Kei Miyakawa, Sayaka Shizukuishi, Koichi Watashi, Takaji Wakita, Akihide Ryo
  • Organizer: 2018 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] Anti-HBV activity of novel pyrimidotriazinone derivatives through the inhibition of viral nucleocapsid assembly (2018)
  • Author(s): Masaaki Toyama, Norikazu Sakakibara, Mika Okamoto, Masanori Ikeda, Koichi Watashi, Takaji Wakita, Masanori Baba
  • Organizer: 2018 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] Interaction between nuclear factor-erythroid 2 related factor 2 and hepatitis B virus (2018)
  • Author(s): Yohei Miyayama, Hitomi Okamura, Machiko Sasai, Yuichi Akahori, Koichi Watashi, Takaji Wakita, Makoto Hijikata
  • Organizer: 2018 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] Acidic polysaccharides isolated from marine algae, ascophyllan and fucoidans, inhibit the early step of hepatitis B virus infection (2018)
  • Author(s): Mikinori Ueno, Masato Nogawa, Rokeya Siddiqui, Koichi Watashi, Takaji Wakita, Takasi Okimura, Shogo Isaka, Tatsuya Oda, Yasuo Ariumi
  • Organizer: 2018 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] Comparative analysis of antiviral activity of sPLA2 against HBV and HCV (2018)
  • Author(s): Hak Hotta, Ming Chen, Chie Aoki-Utsubo, Lin Deng, Yohei Miyayama, Makoto Hijikata, Keiko Shindo, Takeshi Noda, Michinori Kohara, Koichi Watashi, Takaji Wakita
  • Organizer: 2018 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] Flavivirus subviral particles-based DNA vaccine induces neutralizing antibodies against HCV (2018)
  • Author(s): Yato K, Matsuda M, Watanabe N, Nakajima S, Fujimoto A, Watashi K, Aizaki H, Kato T, Muramatsu M, Wakita T, Suzuki R
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] Identification of Aryl hydrocarbon receptor ligands inhibiting the lipid accumulation and Hepatitis C virus production (2018)
  • Author(s): Nishioka K, Ohashi H, Suzuki R, Aizaki H, Muramatsu M, Wakita T, Watashi K
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] Abnormal hepatocellular organelles remain to be observed in sustained virological response (SVR) patients (2018)
  • Author(s): Aoyagi H, Iijima H, Zheng X, Kao YT, Matsuda M, Watashi K, Suzuki R, Masaki T, Shimada N, Kato K, Tsubota A, Wake K, Wakita T, Aizaki H
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] Antiviral activity of phospholipase A2 group V (PLA2G5) against HCV (2018)
  • Author(s): Puig-Basagoiti F, Fukasawa M, Suzuki R, Watashi K, Muramatsu M, Wakita T, Aizaki H
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] IL-1b/ATF3 mediated regulation of HBx mRNA decay (2018)
  • Author(s): Aly HH, Watashi K, Watanabe N, Wakita T
  • Organizer: The 66th Annual Meeting of the Japanese Society for Virology
• [Presentation] Acidic polysaccharides isolated from marine algae, ascophyllan and fucoidans, inhibit the early step pf hepatits B virus infection (2018)
  • Author(s): Ueno M, Nogawa M, Siddiqui R, Watashi K, Wakita T, Okimura T, Isaka S, Oda T, Ariumi Y
  • Organizer: The 66th Annual Meeting of the Japanese Society for Virology
• [Presentation] Identification of host kinases that regulate hepatitis B virus internalization (2018)
  • Author(s): Iwamoto M, Saso W, Sugiyama M, Ishii K, Suzuki R, Aizaki H, Ryo A, Ohtani N, Muramatsu M, Iwami S, Tanaka Y, Wakita T, Watashi K
  • Organizer: The 66th Annual Meeting of the Japanese Society for Virology
• [Presentation] Isolation and structure determination of antiviral natural products from fungi (2018)
  • Author(s): Kamisuki S, Matsunaga H, Nishikori S, Takemoto K, Kaneko M, Nakajima S, Murakami M, Murakami H, Kuramochi K, Watashi K, Sugawara F
  • Organizer: American Society of Pharmacognosy 2018 Annual Meeting
  • Int'l Joint Research
• [Presentation] Novel predictors of the treatment outcome of Hepatitis B patients treated with entecavir and lamivudine nucleos(t)ide analogues (2018)
  • Author(s): Alexey Martyushev, Shingo Iwami, Koichi Watashi
  • Organizer: 2018 Annual Meeting of the Society for Mathematical Biology & the Japanese Society for Mathematical Biology
  • Int'l Joint Research
• [Presentation] B型肝炎ウイルス培養系の構築から創薬研究へ (2018)
  • Author(s): 渡士 幸一
  • Organizer: 日本薬学会第138年会
  • Invited
• [Presentation] SLC10A1/NTCPのトラフィッキング制御を標的とした新規B型肝炎ウイルス感染阻害戦略 (2018)
  • Author(s): 深野 顕人, 九十田 千子, 鈴木 亮介, 相崎 英樹, 村松 正道, 脇田 隆字, 小笠原 裕樹, 渡士 幸一
  • Organizer: 日本薬学会第138年会
• [Presentation] 生理活性化合物を利用した肝炎ウイルス生活環の解析 (2017)
  • Author(s): 渡士 幸一
  • Organizer: 国立成育医療研究センターセミナ-
  • Invited
• [Presentation] B型肝炎ウイルスの培養系と創薬研究 (2017)
  • Author(s): 渡士 幸一
  • Organizer: 第１９回 仙台 Liver Meeting
  • Invited
• [Presentation] 肝細胞内脂肪滴および脂質膜構造によるC型肝炎ウイルス増殖制御機構の解析 (2017)
  • Author(s): 渡士 幸一
  • Organizer: 第２８回 小野医学研究財団 研究成果発表会
  • Invited
• [Presentation] Ｂ型肝炎ウイルス基礎研究・創薬研究の最近の知見 (2017)
  • Author(s): 渡士 幸一
  • Organizer: 衛生微生物技術協議会第38回研究会
  • Invited
• [Presentation] トランスポーターと肝炎ウイルス (2017)
  • Author(s): 渡士 幸一
  • Organizer: 日本薬物動態学会第32回年会東京
  • Invited
• [Presentation] Targeting HBV-X by regulating Non-Stop mediated RNA decay (2017)
  • Author(s): Hussein H. Aly, Fumihiro Shiromoto, Takanobu Kato, Koichi Watashi, Kazuaki Chayama, Takaji Wakita
  • Organizer: 5th JAPAN-TAIWAN-KOREA HBV Research Symposium 2017
  • Int'l Joint Research
• [Presentation] Identification of HBV entry inhibitors without interfering with the NTCP transporter activity (2017)
  • Author(s): Kento Fukano, Satomi Shimura, Michael Peel, Ann Sluder, Fumihiro Kawai, Senko Tsukuda, Ryosuke Suzuki, Hideki Aizaki, Sam-Yong Park, Takaji Wakita, Yuki Ogasawara, Koichi Watashi
  • Organizer: 5th JAPAN-TAIWAN-KOREA HBV Research Symposium 2017
  • Int'l Joint Research
• [Presentation] 抗HBV効果を有するNeplanocin A誘導体の作用機序解析 (2017)
  • Author(s): 外山政明, 濱崎隆之, 岡本実佳, 渡士幸一, 脇田隆字, Ashoke Sharon, 馬場昌範
  • Organizer: 第27回抗ウイルス療法学会学術集会・総会
• [Presentation] 抗肝炎ウイルス薬の薬効・変異頻度定量評価系の開発と多剤併用の意義 (2017)
  • Author(s): 渡士幸一, 小泉吉輝, 大橋啓史, 中嶋翔, 田中靖人, 脇田隆字, Alan S. Perelson, 岩見真吾
  • Organizer: 第27回抗ウイルス療法学会学術集会・総会
• [Presentation] C型慢性肝炎のウイルス学的著効（SVR）後の肝実質細胞のオルガネラ異常の解析 (2017)
  • Author(s): 青柳東代, 飯島尋子, Francesc Puig-Basagoiti, Zheng Xin, Yu Ting Kao, Gewaid E. Hossam, 在津拓馬, 松田麻未, 渡士幸一, 鈴木亮介, 政木隆博, 島田紀明, 加藤慶三, 坪田昭人, 三又絢子, 酒巻有里子, 市野瀬志津子, 和氣健二郎, 脇田隆字, 相崎英樹
  • Organizer: 第27回抗ウイルス療法学会学術集会・総会
• [Presentation] B型肝炎ウイルス培養系を用いた創薬研究 (2017)
  • Author(s): 渡士幸一、九十田千子、脇田隆字
  • Organizer: 第53回日本肝臓学会総会
• [Presentation] 胆汁酸代謝調節機構を標的としたB型肝炎ウイルス制御の試み (2017)
  • Author(s): 伊藤清顕、渡士幸一、米田政志
  • Organizer: 第53回日本肝臓学会総会
• [Presentation] HCVの肝星細胞への感染および肝星細胞活性化のメカニズム解析 (2017)
  • Author(s): 相崎英樹、渡士幸一、鈴木哲朗、政木隆博、横山寛、脇田隆字
  • Organizer: 第53回日本肝臓学会総会
• [Presentation] Abnormal organelles were continuously observed in sustained virological response (SVR) patients (2017)
  • Author(s): Haruyo Aoyagi, Hiroko Iijima, Francesc Puig-Basagoiti, Zheng Xin, Yu Ting Kao, Gewaid E. Hossam, Takuma Zaitsu, Mami Matsuda, Koichi Watashi, Ryosuke Suzuki, Takahiro Masaki, Noritomo Shimada, Keizo Kato, Akihito Tsubota, Ayako Mimata, Yuriko Sakamaki, Shizuko Ichinose, Kenjiro Wake, Takaji Wakita, Hideki Aizaki
  • Organizer: The Asian Pacific Associaton for the Study of the Liver - Single Topic Conference 2017 Nagasaki
  • Int'l Joint Research
• [Presentation] Sphingomyelin participates in the formation of Hepatitis C virus membrane replication vesicles (2017)
  • Author(s): Hossam E. GEWAID, Haruyo AOYAGI, Minetaro ARITA, Koichi WATASHI, Ryosuke SUZUKI, Hussein ALY, Keigo KUMAJAI, Toshiyuki YAMAJI, Masayoshi FUKASAWA, Ayako MIMATA, Yuriko SAKAMAKI, Shizuko ICHINOSE, Kentaro HANADA, Takaji WAKITA, Hideki AIZAKI
  • Organizer: American Society for Virology - 2017 Annual Meeting
  • Int'l Joint Research
• [Presentation] The integrity of cellular microtubule structure is important for an efficient HBV capsid formation (2017)
  • Author(s): Iwamoto M, Cai D, Sugiyama M, Suzuki R, Aizaki H, Ryo A, Ohtani N, Tanaka Y, Mizokami M, Wakita T, Guo H, Watashi K
  • Organizer: 2017 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] Supporting evidence that HBV virions bud mainly through the ER/ERGIC membranes (2017)
  • Author(s): Hotta H, Chen M, Aoki-Utsubo C, Deng L, Watashi K, Wakita T
  • Organizer: 2017 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] HBV-X and the balance between degradation or inflammation (2017)
  • Author(s): Aly H, Shiromoto F Watashi K, Hoshino S, Kato T, Chayama K, Wakita T
  • Organizer: 2017 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] HBV exploit Ski2/RNA complex to degrade cytokines and suppress inflammation (2017)
  • Author(s): Aly HH, Shiromoto F, Watashi K, Kato T, Chayama K, Wakita T
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Membrane protein, embryonic lethal, abnormal vision, drosophila-like 1, interacts with NS5A and involves in hepatitis C virus replication (2017)
  • Author(s): Kao YT, Goto K, Aoyagi H, Hossam GE, Zheng X, Puig-Basagoiti F, Watashi K, Suzuki R, Yamagoe S, Dohmae N, Suzuki T, Okushin K, Tsutsumi T, Yotsuyanagi H, Moriya K, Koike K, Suzuki T, Wakita T, Aizaki H
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] The bidirectional interplay of hepatitis B virus and cell-death inducing DFF45-like effectors (2017)
  • Author(s): Kasai H, Yasumoto J, Yamashita A, Tanaka T, Watashi K, Wakita T, Otoguro T, Moriishi K
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Novel crosstalk of Xenobiotic Response with Lipid Biosynthesis Pathway regulates the Host Permissiveness to the Hepatitis C Virus Production (2017)
  • Author(s): Ohashi H, Nakajima S, Kim S, Fukasawa M, Suzuki R, Aizaki H, Sugawara F, Ohtani N, Wakita T, Watashi K
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Identification of a hepatitis B virus internalization inhibitor with a novel mode of action (2017)
  • Author(s): Fukano K, Tsukuda S, Suzuki R, Aizaki H, Wakita T, Ogasawara Y, Watashi K
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Interaction of hepatitis B virus core protein with microtubles is important for the efficient capsid formation (2017)
  • Author(s): Iwamoto M, Cai D, Sugiyama M, Suzuki R, Aizaki H, Ryo A, Ohtani N, Tanaka Y, Mizokami M, Wakita T, Watashi K
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Phosphorylation of protein kinase R and translational suppression by NS5B protein of hepatitis C virus (2017)
  • Author(s): Suzuki R, Matsuda M, Shimoike T, Watashi K, Aizaki H, Kato T, Suzuki T, Wakita T
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Generation of hepatitis C virus vaccine antigen based on Japanese encephalitis virus subviral particles (2017)
  • Author(s): Yato K, Matsuda M, Watanabe N, Nakajima S, Fujimoto A, Watashi K, Aizaki H, Kato T, Tamura K, Wakita T, Suzuki R
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Establishment of quantification method for the efficacy of anti-hepatitis virus combination treatments (2017)
  • Author(s): Watashi K, Koizumi Y, Ohashi H, Nakajima S, Tanaka Y, Wakita T, Perelson AS, Iwami S
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] NTCPを利用したHBV創薬研究およびNTCPを介したHBV侵入機構の解析 (2017)
  • Author(s): 九十田千子、渡士幸一、小嶋聡一、脇田隆字
  • Organizer: 2017年度 生命科学系合同年次大会
  • Invited
• [Presentation] Involvement of cell-death inducing DFF45-like effectors in HBV replication (2017)
  • Author(s): Jun Yasumoto, Hirotake Kasai, Kentaro Yoshimura, Teruhime Otoguro, Koichi Watashi, Takaji Wakita, Atsuya Yamashita, Tomohisa Tanaka, Sen Takeda, Kohji Moriishi
  • Organizer: 5th JAPAN-TAIWAN-KOREA HBV Research Symposium 2017
  • Int'l Joint Research
• [Presentation] Hepatitis B virus capsid formation is regulated by microtubules and their posttranslational modification (2017)
  • Author(s): Masashi Iwamoto, Masaya Sugiyama, Ryosuke Suzuki, Hideki Aizaki, Yasuhito Tanaka, Masashi Mizokami, Naoko Ohtani, Takaji Wakita, Koichi Watashi
  • Organizer: 5th JAPAN-TAIWAN-KOREA HBV Research Symposium 2017
  • Int'l Joint Research
• [Presentation] B型肝炎ウイルスの内在化阻害剤の同定 (2017)
  • Author(s): Kento Fukano, Senko Tsukuda, Ryosuke Suzuki, Hideki Aizaki, Takaji Wakita, Yuki Ogasawara, Koichi Watashi
  • Organizer: 第14回ウイルス学キャンプin湯河原
• [Presentation] Chemical array system for identifying anti-hepatitis B virus entry inhibitors (2017)
  • Author(s): 九十田千子、金子学、渡士幸一、小嶋聡一、脇田隆字
  • Organizer: 第14回ウイルス学キャンプin湯河原
• [Presentation] Crosstalk of Xenobiotic Metabolism Pathway with Lipid Metabolism regulates the Host Permissiveness to Hepatitis C Virus Production (2017)
  • Author(s): Hirofumi Ohashi, Syo Nakajima, Sulyi Kim, Ryosuke Suzuki, Hideki Aizaki, Masayoshi Fukasawa, Shinji Kamisuki, Fumio Sugawara, Naoko Ohtani, Takaji Wakita, Koichi Watashi
  • Organizer: 第14回ウイルス学キャンプin湯河原
• [Presentation] 培養細胞を用いたB型肝炎ウイルス cccDNAノ動態解析 (2017)
  • Author(s): Masashi Iwamoto, Yusuke Kakizoe, Shingo Iwami, Koichi Watashi
  • Organizer: 第14回ウイルス学キャンプin湯河原
• [Presentation] Antiviral effect of bile acid derivatives on hepatitis B virus infection (2017)
  • Author(s): Okumura A, Ito K, Watashi K, Takeuchi J, Inoue R, Suzuki S, Sakamoto K, Okutsu S, Umezawa K, Wakita T, Yoneda M
  • Organizer: 2017 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] Modulation of HBV life cycle by sulforaphane (2017)
  • Author(s): Okamura H, Sasai M, Nio Y, Watashi K, Wakita T, Hijikata M
  • Organizer: 2017 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] Fatty acids with certain carbon chain length can support hepatitis B virus production (2017)
  • Author(s): Okamura H, Nio Y, Watashi K, Wakita T, Hijikata M
  • Organizer: 2017 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] An Epidemiology Study of Acute Hepatitis B in Japan, April 1999 ~ December 2015 (2017)
  • Author(s): Zheng X, Aoyagi H, Hossam G, Zaitsu T, Puig-Basagoiti F, Kao Y, Watashi K, Suzuki R, Takahashi T, Sunagawa T, Oishi K, Wakita T, Aizaki H
  • Organizer: 2017 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] Structural Proteins of Hepatitis C virus Genotype 3a S310 Strain Permit Claudin-1- independent Entry (2017)
  • Author(s): Suzuki R, Yato K, Tosaka T, Hmwe SS, Matsuda M, Watashi K, Aizaki H, Fukasawa M, Matsuura Y, Wakita T
  • Organizer: 24th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] NS5A-Associated Membrane Protein, Embryonic Lethal, Abnormal Vision, Drosophila-Like 1, Involves in Hepatitis C Replication (2017)
  • Author(s): Kao YT, Goto K, Aoyagi H, Hossam GE, Zheng X, Puig-Basagoiti F, Watashi K, Suzuki R, Yamagoe S, Dohmae N, Suzuki T, Okushin K, Tsutsumi T, Yotsuyanagi H, Moriya K, Koike K, Suzuki T, Wakita T, Aizaki H
  • Organizer: 24th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] Novel Crosstalk of Xenobiotic Response with Lipid Metabolism Regulates the Host Permissivenessto Hepatitis C Virus Production (2017)
  • Author(s): Ohashi H, Nakajima S, Kim S, Suzuki R, Aizaki H, Fukasawa M, Kamisuki S, Sugawara F, Ohtani N, Wakita T, Watashi K
  • Organizer: 24th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] Broad antiviral activities of acidic polysaccharides isolated from marine algae (2017)
  • Author(s): Ueno M, Nogawa M, Siddiqui R, Watashi K, Wakita T, Kato T, Oda T, Ariumi Y
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Analysis of HBV life cycle in the HepG2 expressing human NTCP cell line (2017)
  • Author(s): Rahayu R, Ohsaki E, Honda T, Okamoto T, Watashi K, Ueda K
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Fatty acids synthesized from cellular fatty acid synthesis pathway play roles in hepatitis B virus production (2017)
  • Author(s): Okamura H, Nio Y, Watashi K, Wakita T, Hijikata M
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Human hepatic stellate cells are permissive for hepatitis C virus infection/replication and play important roles in fibrosis (2017)
  • Author(s): Zheng X, Zaitsu T, Aoyagi H, Matsuda M, Watanabe N, Fujimoto A, Watashi K, Suzuki R, Fukuhara T, Matsuura Y, Ichinose S, Wake K, Suzuki T, Iijima H, Yokoyama H, Masaki T, Matsuura T, Tamura K, Wakita T, Aizaki H
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Abnormal hepatocellular organelles remain to be observed in sustained virological response patients (2017)
  • Author(s): Aoyagi H, Iijima H, Puig-Basagoiti F, Zheng X, Kao YT, Hossam GE, Matsuda M, Watashi K, Suzuki R, Masaki T, Aizawa N, Shimada N, Kato K, Tsubota A, Mimata A, Sakamaki Y, Ichinose S, Wake K, Wakita T, Aizaki H
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Screening of FDA-approved drugs activating cyclic GMP-AMP synthase promoter (2017)
  • Author(s): Imai H, Dansako H, Ueda Y, Satoh S, Watashi K, Wakita T, Kato N
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Establishment of a neutralization assay for multiple flaviviruses based on single-round infectious particles (2017)
  • Author(s): Matsuda M, Yamanaka A, Yoshii K, Watashi K, Aizaki H, Konishi E, Takasaki T, Wakita T, Suzuki R
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Identification of a new class of HBV and HDV entry inhibitor, proanthocyanidin (2017)
  • Author(s): Senko Tsukuda, Koichi Watashi, Soichi Kojima, Takaji Wakita
  • Organizer: AASLD The Liver Meeting 2017
  • Int'l Joint Research
• [Book] Encyclopedia of Signaling Molecules, 2nd Edition (2017)
  • Author(s): Tsukuda S, Iwamoto M, Watashi K
  • Publisher: Springer Nature
• [Patent(Industrial Property Rights)] HBV感染阻害剤 (2019)
  • Inventor(s): 渡士幸一、脇田隆字、村松正道
  • Industrial Property Rights Holder: 渡士幸一、脇田隆字、村松正道
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2019-033601
  • Filing Date: 2019
• [Patent(Industrial Property Rights)] NTCPを標的とした抗B型肝炎ウイルス剤 (2018)
  • Inventor(s): 菅裕明、トビー パシオウラ、脇田隆字、渡士幸一
  • Industrial Property Rights Holder: 菅裕明、トビー パシオウラ、脇田隆字、渡士幸一
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2018-002814
  • Filing Date: 2018