| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2019: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2017: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Outline of Final Research Achievements |
unx/Cbfb transcription factor complexes are known to play essential roles in regulating development of multiple types of cells. Our preliminary works showed that C-terminal end sequences of Runx and Cbfb2 are important for the function of Runx/Cbfb complexes. In this study, we focuses on the last Y residue in the Runx3 protein and replaced the Y with W, F or E residue. Analyses of these mutant lines suggested that the Y residue might be phosphorylated and this post-translational modification is quite essential for regulating the function of Runx protein.
As for the functional characterization of Cbfb2-specific C-terminal amino-acid sequences, proteomics approach and analyses of a new mutant strain lacking the above Cbfb2-specific C-terminal sequences failed to find Cbfb2-specific interacting partners and function, respectively. These finding suggest that RNA splicing at the Cbfb locus might contribute to regulate total amount of Cbfb protein.
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