Practical application of biomonitoring method for understanding the exposure of pyrethroid for home use

• Project/Area Number: 17H04132
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hygiene and public health
• Research Institution: Nagoya University
• Principal Investigator: Ueyama Jun 名古屋大学, 医学系研究科(保健), 准教授 (00397465)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2017: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
• Keywords: ピレスロイド系殺虫剤 / バイオモニタリング / 尿中代謝物 / 家庭用殺虫剤 / 室内環境測定 / 曝露 / 曝露評価

Outline of Final Research Achievements

Pyrethroid (PYR) is one of the main insecticides used not only in agriculture fields but also household environments and public places. We focused on the exposure assessment of transfluthrin, metofluthrin, profluthrin, and the other PYRs. A sensitive and efficient procedure for the high-throughput determination of nine urinary metabolites of pyrethroids such as 2,3,5,6-tetrafluorobenzyl alcohol (FB-Al), 4-methyl-2,3,5,6-tetrafluorobenzyl alcohol (CH3-FB-Al), 4-methoxymethyl-2,3,5,6-tetrafluorobenzyl alcohol (CH3OCH2-FB-Al), and 2,3,5,6-tetrafluoro-1,4-benzenedimethanol (HOCH2-FB-Al) has been developed using GC-MS/MS.Moreover, we revealed that exposure level to some PYRs has increased over the past decade, and that children are exposed to higher levels of hygiene-PYRs in summer than in winter.

Academic Significance and Societal Importance of the Research Achievements

これまでに一般生活者集団の尿から新規に開発されたピレスロイド系殺虫剤の曝露マーカーである尿中代謝物を定量分析した報告はない。本研究によって一般生活者集団から曝露マーカーをモニタリングできるようになり、日常的なピレスロイド系殺虫剤の曝露レベルを個人レベルで把握することを可能にした。この成果は、ピレスロイド系殺虫剤を含有する虫ケア商品などの、安全でかつ有効な使用に向けた使用方法あるいは法規制の立案に貢献できるものと思われる。

Research Products

• [Int'l Joint Research] 上海交通大学(中国)
• [Journal Article] Ten-year temporal trends (2006?2015) and seasonal-differences in urinary metabolite concentrations of novel, hygiene-used pyrethroids in Japanese children (2020)
  • Author(s): Hamada Risa、Ueda Yuko、Wada Keiko、Saito Isao、Nomura Hiroshi、Kamijima Michihiro、Nakane Kunihiko、Nagata Chisato、Kondo Takaaki、Ueyama Jun
  • Journal Title: International Journal of Hygiene and Environmental Health Volume: 225 Pages: 113448-113448
  • DOI: 10.1016/j.ijheh.2019.113448
  • Peer Reviewed
• [Journal Article] Freeze-drying enables homogeneous and stable sample preparation for determination of fecal short-chain fatty acids (2020)
  • Author(s): Ueyama Jun、Oda Masaya、Hirayama Masaaki、Sugitate Kuniyo、Sakui Norihiro、Hamada Risa、Ito Mikako、Saito Isao、Ohno Kinji
  • Journal Title: Analytical Biochemistry Volume: 589 Pages: 113508-113508
  • DOI: 10.1016/j.ab.2019.113508
  • Peer Reviewed
• [Journal Article] Exposure levels of organophosphate pesticides in Japanese diapered children: Contributions of exposure-related behaviors and mothers’ considerations of food selection and preparation (2020)
  • Author(s): Oya Naoko、Ito Yuki、Ebara Takeshi、Kato Sayaka、Hioki Keisuke、Aoi Arisa、Ueyama Jun、Oguri Tomoko、Shoji Naoto、Sugiura-Ogasawara Mayumi、Saitoh Shinji、Kamijima Michihiro
  • Journal Title: Environment International Volume: 134 Pages: 105294-105294
  • DOI: 10.1016/j.envint.2019.105294
  • Peer Reviewed / Open Access
• [Journal Article] Association between Redox State of Human Serum Albumin and Exercise Capacity in Elderly Women: A Cross-Sectional Study (2020)
  • Author(s): Ashikawa H，Adachi T, Ueyama J, Yamada S
  • Journal Title: Geriatr Gerontol Int Volume: 20 Issue: 3 Pages: 256-260
  • DOI: 10.1111/ggi.13849
  • Peer Reviewed
• [Journal Article] Occupational exposure limits for cumene, 2,4-dichlorophenoxy acetic acid, silicon carbide whisker, benzyl alcohol, and methylamine, and carcinogenicity, occupational sensitizer, and reproductive toxicant classifications (2019)
  • Author(s): The Committee for Recommendation of Occupational Exposure Limits, Japan Society for Occupational Health
  • Journal Title: Journal of Occupational Health Volume: 61 Issue: 4 Pages: 328-330
  • DOI: 10.1002/1348-9585.12073
  • NAID: 130007683730
  • ISSN: 1341-9145, 1348-9585
  • Year and Date: 2019-07-20
  • Peer Reviewed / Open Access
• [Journal Article] Prenatal exposure to organophosphate pesticides, maternal paraoxonase 1 genotype, and childhood neurodevelopment at 24 months of age in Shandong, China (2019)
  • Author(s): Wang Yiwen、Zhang Yan、Ji Lin、Zhou Yijun、Shi Rong、Kamijima Michihiro、Ueyama Jun、Gao Yu、Tian Ying
  • Journal Title: Environmental Science and Pollution Research Volume: 27 Issue: 2 Pages: 1969-1977
  • DOI: 10.1007/s11356-019-06740-4
  • Int'l Joint Research
• [Journal Article] Biomonitoring method for neonicotinoid insecticides in urine of non-toilet-trained children using LC-MS/MS (2019)
  • Author(s): Ueyama Jun、Aoi Arisa、Ueda Yuko、Oya Naoko、Sugiura Yuka、Ito Yuki、Ebara Takeshi、Kamijima Michihiro
  • Journal Title: Food Additives & Contaminants: Part A Volume: 37 Issue: 2 Pages: 304-315
  • DOI: 10.1080/19440049.2019.1696020
  • NAID: 120006808760
  • Peer Reviewed
• [Journal Article] Cohort profile: Aichi regional sub-cohort of the Japan Environment and Children’s Study (JECS-A) (2019)
  • Author(s): Ebara Takeshi、Yamada Yasuyuki、Shoji Naoto、Ito Yuki、Nakagawa Atsuko、Miyachi Taishi、Ozaki Yasuhiko、Omori Toyonori、Suzuki Sadao、Kojima Masayo、Ueyama Jun、Tomizawa Motohiro、Kato Sayaka、Oguri Tomoko、Matsuki Taro、Sato Hirotaka、Oya Naoko、Sugiura-Ogasawara Mayumi、Saitoh Shinji、Kamijima Michihiro
  • Journal Title: BMJ Open Volume: 9 Issue: 11 Pages: e028105-e028105
  • DOI: 10.1136/bmjopen-2018-028105
  • Peer Reviewed / Open Access
• [Journal Article] 93.Human serum albumin redox state is associated with decreased renal function in a community-dwelling population. (2019)
  • Author(s): Fujii R, Ueyama J, Kanno T, Suzuki K, Hamajima N, Wakai K, Hasegawa Y, Kondo T.
  • Journal Title: Am J Physiol Renal Physiol. 2019 Jan 1;316(1):F214-F218 Volume: 316 Issue: 1 Pages: 214-218
  • DOI: 10.1152/ajprenal.00138.2018
  • Peer Reviewed
• [Journal Article] Association between exposure to household smoking and dental caries in preschool children: a cross-sectional study. (2019)
  • Author(s): Goto Y, Wada K, Konishi K, Uji T, Koda S, Mizuta F, Yamakawa M, Watanabe K, Ando K, Ueyama J, Kondo T, Nagata C.
  • Journal Title: Environ Health Prev Med. Volume: 24(1) Issue: 1 Pages: 9-9
  • DOI: 10.1186/s12199-019-0764-1
  • NAID: 210000130327
  • Peer Reviewed / Open Access
• [Journal Article] Intra-individual variations of organophosphate pesticide metabolite concentrations in repeatedly collected urine samples from pregnant women in Japan (2019)
  • Author(s): Hioki Keisuke、Ito Yuki、Oya Naoko、Nakayama Shoji F.、Isobe Tomohiko、Ebara Takeshi、Shibata Kanemitsu、Nishikawa Naomi、Nakai Kunihiko、Kamida Tomota、Ueyama Jun、Sugiura-Ogasawara Mayumi、Kamijima Michihiro
  • Journal Title: Environmental Health and Preventive Medicine Volume: 24 Issue: 1 Pages: 7-7
  • DOI: 10.1186/s12199-019-0761-4
  • Peer Reviewed / Open Access
• [Journal Article] Within-individual and interlaboratory variability analyses of urinary metabolites measurements of organophosphorus insecticides (2019)
  • Author(s): Ito Yuki、Ueyama Jun、Nakayama Shoji F、Isobe Tomohiko、Oya Naoko、Sato Hirotaka、Ebara Takeshi、Yoshimasu Kouichi、Tsuno Kanami、Tatsuta Nozomi、Nakai Kunihiko、Kamijima Michihiro
  • Journal Title: Journal of Exposure Science & Environmental Epidemiology Volume: in press Issue: 4 Pages: 721-729
  • DOI: 10.1038/s41370-019-0124-7
  • Peer Reviewed
• [Journal Article] Occupational Exposure Limits for ethylidene norbornene, ethyleneimine, benomyl, and 2,3-epoxypropyl methacrylate, and classifications on carcinogenicity (2018)
  • Author(s): Committee for Recommendation of Occupational Exposure Limits, Japan Society for Occupational Health, Araki A, Azuma K, Endo G, Endo Y, Fukushima T, Hara K, Hori H, Horie S, Horiguchi H, Ichiba M, Ichihara G, Ikeda M, Ishitake T, Ito A, Ito Y, Iwasawa S, Kakumu T, Kamijima M, Karita K.
  • Journal Title: Journal of Occupational Health Volume: 60 Issue: 4 Pages: 333-335
  • DOI: 10.1539/joh.2018-0137-OP
  • NAID: 130007420038
  • ISSN: 1341-9145, 1348-9585
  • Year and Date: 2018-07-20
  • Peer Reviewed / Open Access
• [Journal Article] A new method for simultaneous quantification of fosphenytoin, phenytoin and its primary metabolite 5-(4-hydroxyphenyl)-5-phenylhydantoin in whole blood by ultra-performance liquid chromatography-tandem mass spectrometry (2018)
  • Author(s): Suzuki Takayoshi、Ogawa Tadashi、Ueyama Jun、Iwai Masae、Kondo Fumio、Seno Hiroshi
  • Journal Title: Legal Medicine Volume: 34 Pages: 64-69
  • DOI: 10.1016/j.legalmed.2018.08.006
  • Peer Reviewed
• [Journal Article] A sensitive and efficient procedure for the high-throughput determination of nine urinary metabolites of pyrethroids by GC-MS/MS and its application in a sample of Japanese children (2018)
  • Author(s): Ueda Yuko、Oda Masaya、Saito Isao、Hamada Risa、Kondo Takaaki、Kamijima Michihiro、Ueyama Jun
  • Journal Title: Analytical and Bioanalytical Chemistry Volume: 410 Issue: 24 Pages: 6207-6217
  • DOI: 10.1007/s00216-018-1229-x
  • Peer Reviewed
• [Journal Article] Common variant of BCAS3 is associated with gout risk in Japanese population: the first replication study after gout GWAS in Han Chinese. (2018)
  • Author(s): Sakiyama M, Matsuo H, Nakaoka H, Kawamura Y, Kawaguchi M, Higashino T, Nakayama A, Akashi A, Ueyama J, Kondo T, Wakai K, Sakurai Y, Yamamoto K, Ooyama H, Shinomiya N.
  • Journal Title: BMC Med Genet. Volume: 19 Issue: 1 Pages: 96-96
  • DOI: 10.1186/s12881-018-0583-z
  • Peer Reviewed / Open Access
• [Presentation] 松くい虫防除で散布されたフェニトロチオンの作業者暴露と尿中バイオマーカーの関係 (2020)
  • Author(s): 阿部智早絵，丸 諭，上山 純, 斎藤 勲, 本山直樹
  • Organizer: 日本農薬学会第45回大会
• [Presentation] バイオモニタリングを用いた除草剤グリホサートの曝露評価に向けた高感度尿中グリホサート測定法の開発 (2020)
  • Author(s): 野村洸司、浜田梨沙、斎藤勲、近藤高明、佐藤光夫、上島通浩、上山純
  • Organizer: 第90回日本衛生学会学術総会
• [Presentation] Characteristics of Urinary Metabolites as a Biomarker of Household Pyrethroid Insecticides (2019)
  • Author(s): R. Hamada, K. Wada, M. Oda, Y. Ueda, I. Saito, M. Kamijima, K. Nakane, T. Kondo, C. Nagata, J. Ueyama
  • Organizer: 11th International Symposium on Biological Monitoring in Occupational and Environmental Health
  • Int'l Joint Research
• [Presentation] 害虫忌避剤ディートの尿中代謝物高感度測定法の開発 (2018)
  • Author(s): 上山純、上瀬佳菜、浜田梨沙、中根邦彦、近藤高明、佐藤光夫、斎藤勲
  • Organizer: 第89回日本衛生学会学術総会
• [Presentation] 日本人小児における尿中含フッ素芳香族ピレスロイド系殺虫剤代謝物濃度の経年推移 (2018)
  • Author(s): 浜田梨沙、和田恵子、市川みずき、上田裕子、小田真也、 上島通浩、近藤高明、永田知里、斎藤勲、上山純
  • Organizer: 第45回日本毒性学会学術年会
• [Presentation] Development of the urine storage and high-throughput analytical methods for biomonitoring of pyrethroids for large-scale epidemiological studies (2018)
  • Author(s): Jun Ueyama, Risa Hamada, Honoka Kishida, Shinya Oda, Mitsuo Satoh, Hirotaka Sato, Michihiro Kamijima, Isao Saito
  • Organizer: 第21回環境ホルモン学会研究発表会
• [Presentation] バイオモニタリング手法を用いた日本人小児ピレスロイド系殺虫剤曝露レベルの国際比較 (2018)
  • Author(s): 上山 純、上田裕子、伊藤由起、榎原 毅、大矢奈穂子、加藤沙耶香、小栗朋子、庄司直人、齋藤伸治、上島通浩
  • Organizer: 第８８回 日本衛生学会学術総会
• [Presentation] 新規ピレスロイド系殺虫剤代謝物の3歳児尿中濃度に関する基礎的調査 (2018)
  • Author(s): 上田裕子、市川みずき、斎藤勲、大坂彩、野村洸司、近藤高明、中根邦彦, 上山純
  • Organizer: 第８８回 日本衛生学会学術総会