Development of self-growing vascular graft using decellularized vascular tissue and human iPS cell-derived vascular cells
| Project/Area Number |
17H04291
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cardiovascular surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
Ikeda Tadashi 京都大学, 医学研究科, 准教授 (40281092)
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| Co-Investigator(Kenkyū-buntansha) |
湊谷 謙司 京都大学, 医学研究科, 教授 (20393241)
岸田 晶夫 東京医科歯科大学, 生体材料工学研究所, 教授 (60224929)
升本 英利 国立研究開発法人理化学研究所, 生命機能科学研究センター, 上級研究員 (70645754)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2017: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Keywords | 血管再生 / iPS細胞 / 移植・再生医療 / 循環器・高血圧 / 再生医学 / バイオテクノロジー / 細胞・組織 / 再生医療 / 脱細胞グラフト / ヒトiPS細胞 |
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| Outline of Final Research Achievements |
In the present study, we aimed to develop methods to prepare xenogeneic decellularized vascular grafts and to re-seed purified human iPS cell-derived vascular endothelial cells on the decellularized grafts to establish a self-growing vascular grafts for clinical use. Here we established a method to prepare highly purified human iPS cell-derived vascular endothelial cells and to recellularize the decellularized grafts. We also found that xenogeneic vascular grafts can be recellularized in the body of xenogeneic animal with fair recellularization.
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| Academic Significance and Societal Importance of the Research Achievements |
今後増加が予想される虚血性心疾患および下肢虚血性疾患に対する外科的バイパス手術における小口径バイパスグラフトは医療材料として今後ますます重要性が増すものと思われる。本研究成果は動物由来の脱細胞組織に対してヒトiPS細胞由来の血管細胞を播種・再細胞化することによる、いわゆる「ヒト化」血管グラフトを量的制限なく供給しうるための基礎研究的基盤を与えるものであり、医療経済を含む社会的な意義が高いものと考えられる。
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] Chronic optical pacing conditioning of h-iPSC engineered cardiac tissues.2019
Author(s)
Dwenger M, Kowalski WJ, Ye F, Yuan F, Tinney JP, Setozaki S, Nakane T, Masumoto H, Campbell P, Guido W, Keller BB.
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Journal Title
J Tissue Eng
Volume: 10
Pages: 204173141984174-204173141984174
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A therapeutic angiogenesis of sustained release of basic fibroblast growth factor using biodegradable gelatin hydrogel sheets in a canine chronic myocardial infarction model.2018
Author(s)
Kumagai M, Minakata K, Masumoto H, Yamamoto M, Yonezawa A, Ikeda T, Uehara K, Yamazaki K, Ikeda T, Matsubara K, Yokode M, Shimizu A, Tabata Y, Sakata R, Minatoya K.
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Journal Title
Heart Vessels
Volume: 33
Issue: 10
Pages: 1251-1257
DOI
Related Report
Peer Reviewed
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