| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Outline of Final Research Achievements |
Recently we established procedures to directly convert human fibroblasts into osteoblasts by transducing some transcription factor genes or by treating the cells with a chemical compound. To apply this technology to bone regenerative therapy, it should be important to determine optimal procedures of the reprogramming, to understand the mechanisms of reprogramming, and to create 3D bone tissue in culture that is suitable for tailor-made autologous transplantation. In this project we explored chemical compounds that inhibit the molecules involved in the direct reprogramming by means of AI-based combinatorial chemistry. We also developed 3D bone tissue in culture using the directly converted osteoblasts. The results offered valuable information to apply the direct reprogramming technology to bone regenerative medicine in the near future.
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