Development of the optimized and fuctional stem cell therapy by stimulating with the exogeneous growth factors

• Project/Area Number: 17H04359
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Juntendo University
• Principal Investigator: Mizuno Hiroshi 順天堂大学, 医学部, 教授 (80343606)
• Co-Investigator(Kenkyū-buntansha): 林 礼人 順天堂大学, 医学部, 教授 (10365645) ; 飛田 護邦 順天堂大学, 革新的医療技術開発研究センター, 准教授 (10599038) ; 田中 里佳 順天堂大学, 医学部, 先任准教授 (70509827)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2019: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
• Keywords: 再生医学 / 脂肪組織幹細胞 / 増殖因子 / 血管新生

Outline of Final Research Achievements

The purpose of this experiment was to establish the effective therapeutic approach using adipose-derived stem cell for tissue repair and regeneration by accelerating the release of the endogeneous growth factors which can be positive for the regeneration by the pre-stimulation with the exogeneous growth factors. The results showed that several numbers of positive gene expression and the increase of the protein level including IL-8 and CXCL1 by the stimulation with basic fibroblast growth factor were confirmed due to angiogenesis.

Academic Significance and Societal Importance of the Research Achievements

幹細胞を用いた再生医療は、従来幹細胞自身が有する成熟細胞への分化能の他に、幹細胞自身が放出する各種の増殖因子等が相同的に作用して再生効果を高めると考えられてきたが、近年では後者が有力であるのではと示唆されてきた。今回の研究ではbFGF刺激によってASCsから強く発現するタンパク質や遺伝子の網羅的解析を通じて仮説をある程度証明することができた。今後は幹細胞の持つ再生効果を最適化できるような外因性増殖因子やその量について検証していきたい。

Research Products

• [Journal Article] Current status of bone regeneration using adipose-derived stem cells (2018)
  • Author(s): Tajima S, Tobita M and Mizuno H
  • Journal Title: Histol Histopathol Volume: 33 Pages: 619-627
  • Peer Reviewed
• [Journal Article] Multipotency and secretome: the mechanisms behind the regenerative potential of adipose-derived stem cells (2017)
  • Author(s): Orgun D and Mizuno H
  • Journal Title: Plast Aesthet Res Volume: 4 Issue: 3 Pages: 32-40
  • DOI: 10.20517/2347-9264.2016.109
  • Peer Reviewed
• [Presentation] Latest advances for reconstructive and regenerative surgery: The Juntendo experiences (2019)
  • Author(s): Mizuno H
  • Organizer: 2019 Annual Meeting of Taiwan Society of Plastic Surgery
  • Int'l Joint Research / Invited
• [Presentation] 美容医療における再生医療の諸問題 (2019)
  • Author(s): 水野博司
  • Organizer: 第62回日本形成外科学会総会・学術集会
• [Presentation] 自家脂肪注入術の基礎 (2019)
  • Author(s): 水野博司
  • Organizer: 第62回日本形成外科学会総会・学術集会
• [Presentation] Recent trends of adipose derived stem cells for skin restoration, repair and regeneration (2018)
  • Author(s): Mizuno H
  • Organizer: 10th Juntendo University in collaboration with 17th Mae Fah Luang International Conference in Dermatology Aesthetic Dermatology and Anti Aging Medicine (Bangkok, Thailand, 2018)
  • Int'l Joint Research / Invited
• [Presentation] Mechanism of adipose-derived stem cells in regenerative medicine (2018)
  • Author(s): Mizuno H
  • Organizer: 1st Annual Scientific Meeting of REJASELINDO (Surakarta, Indonesia, 2018)
  • Int'l Joint Research / Invited
• [Presentation] Fat grafting in plastic and regenerative surgery (2018)
  • Author(s): Mizuno H
  • Organizer: 1st Annual Scientific Meeting of REJASELINDO (Surakarta, Indonesia, 2018)
  • Int'l Joint Research / Invited
• [Presentation] “Plastic and Regenerative Surgery”: historical review and state-of-the-art clinical science (2018)
  • Author(s): Mizuno H
  • Organizer: 5th TERMIS World Congress 2018 (Kyoto, Japan 2018)
  • Int'l Joint Research
• [Presentation] Multipotency and the secretome: the mechanisms behind the regenerative potential of ASCs (2017)
  • Author(s): Mizuno H and Karibe A
  • Organizer: PRS KOREA 2017
  • Int'l Joint Research / Invited
• [Book] 脂肪注入移植術 (2019)
  • Author(s): 水野博司
  • Total Pages: 135
  • Publisher: 克誠堂出版
  • ISBN: 9784771905191
• [Book] Adipose Derived Stem Cells: Methods and Protocols, Second Edition (2018)
  • Author(s): Tajima S, Tobita M and Mizuno H
  • Total Pages: 284
  • Publisher: Springer, New York, NY
  • ISBN: 9781493977970