Analysis of reparative dentin formation regulated by dental pulp stem cells using a fate mapping approach
| Project/Area Number |
17H04374
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Tokyo Dental College (2018-2019)
Matsumoto Dental University (2017) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
荒井 敦 松本歯科大学, 歯学部, 准教授 (00532772)
細矢 明宏 北海道医療大学, 歯学部, 准教授 (70350824)
小林 泰浩 松本歯科大学, 総合歯科医学研究所, 教授 (20264252)
宇田川 信之 松本歯科大学, 歯学部, 教授 (70245801)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2017: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Keywords | 象牙芽細胞 / 第三象牙質 / 歯髄幹細胞 / ジフテリア毒素 / 細胞の枯渇実験 / フェイトマッピング解析 / 骨芽細胞 / 細胞枯渇実験 / 修復象牙質 / PTH / 骨髄間葉系幹細胞 |
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| Outline of Final Research Achievements |
Tertiary dentin formation is induced in response to dental cavity preparation for dental treatment. In this biological process, cavity preparation induces odontoblastic cell death and fewer odontoblasts are thus regenerated from dental pulp stem cells, resulting in induced reparative dentin formation. However, the relationship between odontoblastic cell death and the regulation of tertiary dentin formation is unclear. In this study, we examined the effects of odontoblastic depletion on dentinogenesis activation using genetically modified mice. We found that dentin formation increased in response to odontoblastic cell death. This result suggests that there is a dental pulp niche environment regulated by odontoblastic cell death and that this regulatory network is essential for the activation of reparative dentin formation.
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| Academic Significance and Societal Importance of the Research Achievements |
歯の修復は、失われた硬組織を補填する生体防御反応であり、臨床現場では、より早期の修復象牙質形成が求められる。しかしながら、これまで硬組織修復のメカニズムはよくわかっていなかった。本研究成果により、象牙芽細胞の細胞死が、歯髄環境に影響を及ぼし、未分化歯髄細胞画分の象牙芽細胞分化を誘導することが明らかになった。今後、以上の実験系を活用することにより、硬組織修復の促進を目指した治療法の提案に繋がることが期待される。
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Report
(4 results)
Research Products
(52 results)
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[Journal Article] Parathyroid Hormone Shifts Cell Fate of a Leptin Receptor-Marked Stromal Population from Adipogenic to Osteoblastic Lineage.2019
Author(s)
Yang M, Arai A, Udagawa N, Zhao L, Nishida D, Murakami K, Hiraga T, Takao-Kawabata R, Matsuo K, Komori T, Kobayashi Y, Takahashi N, Isogai Y, Ishizuya T, Yamaguchi A, Mizoguchi T
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Journal Title
J Bone Miner Res
Volume: 34
Issue: 10
Pages: 1952-1963
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The W9 peptide directly stimulates osteoblast differentiation via RANKL signaling2017
Author(s)
Nakamura, M., Nakamichi, Y., Mizoguchi, T., Koide, M., Yamashita, T., Ara, T., Nakamura, H., Penninger, JM., Furuya, Y., Yasuda, H., and Udagawa, N.
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Journal Title
Journal of Oral Biosciences
Volume: 59
Pages: 146-151
Related Report
Peer Reviewed
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[Presentation] 象牙芽細胞の枯渇は修復象牙質を誘導する2019
Author(s)
趙麗娟, 荒井敦, 堀部寛治, 細矢明宏, 増子倫也, 小林泰浩, 宇田川信之, 高橋直之, 李憲起, 各務秀明, 溝口利英
Organizer
第4回日本骨免疫学会ウインターセミナー
Related Report
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[Presentation] Depletion of odontoblasts induces reparative dentin formation2019
Author(s)
Zhao L., Arai A., Udagawa N., Horibe K., Hosoya A., Masuko R., Okabe K., Shin M., Kobayashi Y., Takahashi N., Li X., Kagami H., Mizoguchi T.
Organizer
The 7th Seoul Symposium on Bone Health in conjunction with the 31th Spring Scientific Congress of Korean Society for Bone and Mineral Research
Related Report
Int'l Joint Research
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[Presentation] Vdr in osteoblast-lineage cells primarily mediates a 1α,25(OH)2D3 derivative-induced increase in bone mass by suppressing bone resorption2018
Author(s)
Nakamichi Y., Udagawa N., Horibe K., Mizoguchi T., Yamamoto Y., Nakamura T., Hosoya A., Kato, S., Suda, T., and Takahashi N.
Organizer
20th Vitamin D Workshop
Related Report
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[Presentation] 破骨細胞に発現するSiglec-15の中和抗体は骨吸収を抑制しながら骨芽細胞の分化を促進する2018
Author(s)
宇田川信之, 小出雅則, 上原俊介, 荒井敦, 溝口利英, 山下照仁, 中村美どり, 小林泰浩, 高橋直之, 熊倉誠一郎, 福田千恵, 津田英資
Organizer
第36回日本骨代謝学会学術集会
Related Report
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[Presentation] Sialic acid-binding immunoglobulin-like lectin 15 (Siglec-15) plays important roles in the induction of both bone-resorbing activity of osteoclasts and osteoblast differentiation.2018
Author(s)
Udagawa N., Koide M., Uehara S., Arai A., Mizoguchi T., Yamashita T., Nakamura M., Kobayashi Y., Takahashi N., Kumakura S., Fukuda C., Tsuda E.
Organizer
Annual Meeting of the American Society for Bone and Mineral Research
Related Report
Int'l Joint Research
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[Presentation] Vdr in osteoblast-lineage cells primarily mediates a 1α,25(OH)2D3 derivative-induced increase in bone mass by suppressing bone resorption2017
Author(s)
Nakamichi, Y., Udagawa, N., Horibe, K., Mizoguchi, T., Yamamoto, Y., Nakamura, T., Hosoya, A., Kato, S., Suda, T., and Takahashi, N.
Organizer
20th Vitamin D Workshop, March, 2017
Related Report
Int'l Joint Research
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