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Comprehensive analysis of human dental biofilms and development of new control methods

Research Project

Project/Area Number 17H04384
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionOsaka University

Principal Investigator

Ebisu Shigeyuki  大阪大学, 歯学研究科, 招へい教授 (50116000)

Co-Investigator(Kenkyū-buntansha) 野杁 由一郎  新潟大学, 医歯学系, 教授 (50218286)
朝日 陽子  大阪大学, 歯学研究科, 助教 (50456943)
岡本 菜々子 (栗木菜々子 / 栗木 菜々子 / 和氣菜々子)  大阪大学, 歯学部附属病院, 医員 (60781432)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywordsデンタルバイオフィルム / in situ / シーケンス解析 / オーラルバイオフィルム / 細菌 / 共焦点レーザー顕微鏡 / 制御 / 次世代シーケンス解析 / 唾液 / 日内変動
Outline of Final Research Achievements

Using in situ biofilm model, it is revealed that the numbers of biofilm-forming bacteria were not affected by sleep and the abundances of obligates anaerobes increased after sleep. Furthermore, it was shown that the expression level of pathogenic genes in biofilm bacteria changed during sleep. In addition, the microbiota in different locations in the oral cavity is affected by sleep and changes depend on the genera of bacteria and on characteristics of the surface on which oral biofilms form.

Academic Significance and Societal Importance of the Research Achievements

デンタルバイオフィルムの病原性を最も効果的にコントロールするための介入時期については未解明な点が多く,これを明らかにするためにはデンタルバイオフィルムの日内変動を理解することが必要不可欠である。本研究により睡眠時におけるデンタルバイオフィルムの変化が明らかとなり,得られた研究成果はより効果的なデンタルバイオフィルムのコントロール法の確立に有意義な示唆を与えるものであると考えられる。

Report

(4 results)
  • 2020 Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (10 results)

All 2021 2020 2019 2018 2017

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (6 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Impacts of sleep on the characteristics of dental biofilm.2021

    • Author(s)
      Sotozono M, Kuriki N, Asahi Y, Noiri Y, Hayashi M, Motooka D, Nakamura S, Machi H, Iida T, Ebisu S.
    • Journal Title

      Sci Rep

      Volume: 11 Issue: 1 Pages: 138-138

    • DOI

      10.1038/s41598-020-80541-5

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Next-Generation Sequencing for Determining the Effect of Arginine on Human Dental Biofilms Using an In Situ Model2021

    • Author(s)
      Kuriki N, Asahi Y, Sotozono M, Machi H, Noiri Y, Hayashi M, Ebisu S.
    • Journal Title

      Pharmacy (Basel).

      Volume: 9(1) Issue: 1 Pages: 18-18

    • DOI

      10.3390/pharmacy9010018

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Assessment of the functional efficacy of root canal treatment with high-frequency waves in rats.2020

    • Author(s)
      Matsui S, Yoneda N, Maezono H, Kuremoto K, Ishimoto T, Nakano T, Yumoto H, Ebisu S, Noiri Y, Hayashi M.
    • Journal Title

      PLoS One.

      Volume: 15 Issue: 9 Pages: 987-996

    • DOI

      10.1371/journal.pone.0239660

    • NAID

      120007188421

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Factors that cause endodontic failures in general practices in Japan.2018

    • Author(s)
      Yamaguchi M, Noiri Y, Itoh Y, Komichi S, Yagi K, Uemura R, Naruse H, Matsui S, Kuriki N, Hayashi M, Ebisu S
    • Journal Title

      BMC Oral Health.

      Volume: 18(1) Issue: 1 Pages: 70-70

    • DOI

      10.1186/s12903-018-0530-6

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Effects of arginine on in situ dental biofilm by NGS2019

    • Author(s)
      Nanako kuriki, Maki Sotozono, Yoko Asahi, Hiroyuki Machi, Yuichiro Noiri, Mikako Hayashi, and Shigeyuki Ebisu
    • Organizer
      IADR
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Impact of Sleeping on Characteristics of in situ Dental Biofilm2019

    • Author(s)
      Maki Sotozono, Nanako kuriki, Yoko Asahi, Hiroyuki Machi, Yuichiro Noiri, Mikako Hayashi, and Shigeyuki Ebisu
    • Organizer
      IADR
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 口腔サンプルを用いて作製したバイオフィルムの実験モデル間の差に関する検討2019

    • Author(s)
      前薗 葉月,クランリアン キッティピット,外園 真規,朝日 陽子, 山口 幹代,栗木 菜々子,野杁 由一郎,林 美加子
    • Organizer
      第150回日本歯科保存学会春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 覚醒時および睡眠時に形成されるデンタルバイオフィルムの三次元的構造解析2018

    • Author(s)
      外園真規,栗木菜々子,朝日陽子,町博之,林美加子,野杁由一郎,恵比須繁之
    • Organizer
      第61回春季歯周病学会学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 睡眠がデンタルバイオフィルムに与える影響2018

    • Author(s)
      外園真規,栗木菜々子,朝日陽子,町博之,林美加子,野杁由一郎,恵比須繁之
    • Organizer
      第32回日本バイオフィルム学会学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 日中および睡眠時に形成されるデンタルバイオフィルムの比較検討 (Comparison of dental biofilm formed in daytime and nighttime)2017

    • Author(s)
      外園真規, 栗木菜々子, 朝日陽子, 町 博之, 林 美加子, 恵比須繁之
    • Organizer
      第147回日本歯科保存学会2017年度秋季学術大会
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2022-01-27  

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