Elucidation of mechanism of sleep bruxism: a high-throughput assay to detect electrophysiological dysfunction using patient-specific iPSCs
| Project/Area Number |
17H04395
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Prosthodontics/ Dental materials science and
|
|---|
| Research Institution | Showa University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
赤松 和土 順天堂大学, 医学(系)研究科(研究院), 教授 (60338184)
美島 健二 昭和大学, 歯学部, 教授 (50275343)
安部 友佳 昭和大学, 歯学部, 講師 (80614156)
松本 貴志 昭和大学, 歯学部, 助教 (00635039)
田中 準一 昭和大学, 歯学部, 講師 (40710166)
戸澤 有理恵 昭和大学, 歯学部, 兼任講師 (70783356)
菅沼 岳史 昭和大学, 歯学部, 教授 (10196694)
|
|---|
| Project Period (FY) |
2017-04-01 – 2020-03-31
|
| Project Status |
Completed (Fiscal Year 2019)
|
| Budget Amount *help |
¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2017: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
|
|---|
| Keywords | 睡眠時ブラキシズム / iPS細胞 / セロトニン2A受容体 / ハイスループット |
|---|
| Outline of Final Research Achievements |
The aim of this study was to elucidate the mechanism of sleep bruxism and to improve the analytical infrastructure based on the technology of patient-specific induced pluripotent stem cells (iPSCs) that a high-throughput assay can be applied to an electrophysiological analysis of SNP-related neural dysfunction. We established iPSCs from both patients with sleep bruxism and healthy controls, and then differentiated to neural cells. Immunostaining was performed with anti-5-HT2A receptor and anti-GABA antibodies. Then electrophysiological recordings were performed on these neurons to record responses following current injection and the bath application of TCB-2. Repetitive action potentials were generated in response to current injection. Inward current was generated by administration of TCB-2, that indicted depolarizing response via 5-HT2A receptor. We successfully established the monitoring system to examine the effect of SB-associated genetic variant.
|
| Academic Significance and Societal Importance of the Research Achievements |
睡眠時ブラキシズム（睡眠中の歯ぎしり）は様々な歯科的問題の原因となり得ますが，メカニズムはまだ不明な点も多く，現在，対症療法のみで対処するのが一般的です．この研究によって確立された解析システムを発展させることで，細胞レベルでの発症メカニズム解明が期待できます．また，この解明が進んで新たな治療法が開発されれば，個々人の状態に合った治療法を選択できるようになると考えられます．
|
Report
(4 results)
Research Products
(27 results)
-
-
-
-
-
[Journal Article] Generation of neural cells using iPSCs from sleep bruxism patients with 5-HT2A polymorphism2017
Author(s)
Hoashi Y, Okamoto S, Abe Y, Matsumoto T, Tanaka J, Yoshida Y, Imaizumi K, Mishima K, Akamatsu W, Okano H, Baba K
-
Journal Title
Journal of Prosthodontic Research
Volume: 61
Issue: 3
Pages: 242-250
DOI
NAID
ISSN
1883-1958, 1883-9207
Related Report
Peer Reviewed
-
-
[Journal Article] Association between patterns of jaw motor activity during sleep and clinical signs and symptoms of sleep bruxism.2016
Author(s)
Yoshida Y, Suganuma T, Takaba M, Ono Y, Abe Y, Yoshizawa S, Sakai T, Yoshizawa A, Nakamura H, Kawana F, Baba K.
-
Journal Title
J Sleep Res.
Volume: 印刷中
Issue: 4
Pages: 415-421
DOI
Related Report
Peer Reviewed
-
[Presentation] Patch-clamp recordings of neurons induced from sleep bruxism patient-specific iPSC2019
Author(s)
Nakai K, Abe Y, Hoashi Y, Nakamura S, Shiga T, Avijite K S, Yasuhara R, Matsumoto T, Kotani K, Inoue T, Mishima K, Akamatsu W, Baba K
Organizer
97th General Session & Exhibition of the IADR
Related Report
Int'l Joint Research
-
[Presentation] Electrophysiological recordings of neurons derived from sleep bruxism patient-specific iPSCs2019
Author(s)
Nakai K, Abe Y, Shiga T, Hoashi Y, Nakamura S, Avijite K S, Yasuhara R, Matsumoto T, Kotani K, Inoue T, Mishima K, Akamatsu W, Baba K
Organizer
ISSCR 2019 Annual Meeting
Related Report
Int'l Joint Research
-
[Presentation] 睡眠時ブラキシズム特異的iPS細胞由来GABA作動性神経細胞の電気生理学的評価2019
Author(s)
中井健人, 安部友佳, 帆足有理恵, Avijite Kumer Sarkar, 小溪啓介, 松本貴志, 安原理佳, 美島健二, 中村史朗, 井上富雄, 志賀孝宏, 赤松和土, 馬場一美
Organizer
日本補綴歯科学会 第128回学術大会
Related Report
-
-
-
-
-
-
-
-
-
[Presentation] In vitro disease modeling for sleep bruxism using induced pluripotent stem cells2017
Author(s)
Tozawa Yurie, Nakai Kento, Yoneima Kazuaki, Tanaka Junichi, Matsumoto Takashi, Abe Yuka, Imaizumi Kento, Mishima Kenji, Akamatsu Wado, Okano Hideyuki, Baba Kazuyoshi
Organizer
65th Annual Meeting of Japanese Association for Dental Research
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
