| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2020: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Outline of Final Research Achievements |
In this study, we first developed methods for generating skeletal cells, including osteoblasts, from human pluripotent stem cells (hPSCs). Gene expression profiling and open chromatin profiling in hPSC-derived endochondral bone tissues further identified cell-type-distinct gene expression profiles and open chromatin regions. Our datasets provide not only epigenome dynamics, but also enhancer regions that govern osteoblast differentiation during human skeletal development. Integrative analyses of the datasets, including cross-species analysis for conservation of target regions, will lead to identification of target enhancer regions for epigenome editing-mediated bone regeneration.
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