Exploration and development of cold-adapted microorganisms useful in low-temperature biotechnology for chemical production and environmental conservation
| Project/Area Number |
17H04598
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 海外学術 |
|---|
| Research Field |
Biofunction/Bioprocess
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2017-04-01 – 2020-03-31
|
| Project Status |
Completed (Fiscal Year 2021)
|
| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
|
|---|
| Keywords | 好冷性微生物 / 低温菌 / 特殊環境 / バイオテクノロジー / 物質生産 / タンパク質生産 / タンパク質分泌生産 / 応用微生物 |
|---|
| Outline of Final Research Achievements |
We developed foreign protein production systems using the cold-adapted bacteria, Shewanella livingstonensis Ac10, Pseudoalteromonas nigrifaciens Sq02, and Shewanella vesiculosa HM13, isolated from low-temperature environments as the hosts. We succeeded in constructing a regulatable low-temperature protein expression system using S. livingstonensis Ac10 and a secretory protein expression system using P. nigrifaciens Sq02.
|
| Academic Significance and Societal Importance of the Research Achievements |
低温菌が生産する好冷性酵素は低温で高い活性を有し、低温で使用される食品加工用酵素や洗剤添加用酵素等として有用と考えられている。しかし、好冷性酵素は一般に熱安定性が低く、常温性酵素などと比べて容易に失活する。本研究で開発した低温菌を宿主としたタンパク質生産系は、タンパク質の熱変性が生じにくい低温での外来タンパク質生産を可能にするものであり、熱安定性の低い好冷性酵素の生産などに有用と考えられる。
|
Report
(4 results)
Research Products
(41 results)
