Study on locul native horse in Asian mountainous area including Himalayan mountains

• Project/Area Number: 17H04636
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Animal production science
• Research Institution: Okayama University
• Principal Investigator: Kunieda Tetsuo 岡山大学, 環境生命科学研究科, 教授 (80178011)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000); Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2018: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: ネパール / 在来家畜 / ウマ / 遺伝的多様性 / Y染色体 / 毛色関連遺伝子 / 在来馬 / mtDNA / ムスタン / 遺伝学 / 畜産学 / ゲノム

Outline of Final Research Achievements

To clarify the genetic characteristics of local native horses in the Himalayan mountains, we performed survey and collection of DNA samples in Mustang, Jumla and Ilam regions of Nepal from 2017 to 2019. Then,we investigated the genetic diversity of Y chromosome, mitochondrial DNA, and coat color-related genes using these horse DNA samples. The results revealed that Y chromosome haplotype of some Nepal native horses have very unique characteristics, mitochondrial DNA haplotypes showed high diversities as other Asian native horses, and there were a relatively high frequency of the allele of TBX3 gene, which is associated with the ancestral Dun coat color of horse.

Academic Significance and Societal Importance of the Research Achievements

ウマは歴史的に運輸、農耕、軍事等の人類の文明の発展に最も深く関わってきた重要な家畜である。歴史的に世界各地で飼育されてきた多様な在来馬の遺伝的特性を明らかにすることは、人類がどの様にウマを家畜化してそれを利用し、さらにそれら家畜化されたウマがどの様に世界各地に伝わってきたかを解明する上で重要な課題である。本研究の成果はこの様な家畜ウマの由来と伝播経路を解明する上で重要であるだけでなく、近年の産業化の進展にともない個体数が減少する中で危惧されているウマの遺伝的多様性の減少を回避し、在来馬として固有の性質を持つウマの集団を維持し保存する上でも重要である。

Research Products

• [Int'l Joint Research] ネパール農業研究機構（NARC)(ネパール)
• [Int'l Joint Research] Nepal Aricultural Reseach Council(ネパール)
• [Journal Article] Genotype distribution and allele frequencies of the genes associated with reproductive traits and hereditary disorders in Japanese native horses. (2019)
  • Author(s): Paul CR, Okuda Y, Nguyen BT, Le NAT, Ibi T, Kawamoto Y, Nozawa K, Kunieda T.
  • Journal Title: J Anim Genet Volume: 47 Pages: 29-36
  • NAID: 130007701689
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Hybrid sterility with meiotic metaphase arrest in intersubspecific mouse crosses. (2019)
  • Author(s): Nishino R, Petri S, Handel MA, Kunieda T, Fujiwara Y.
  • Journal Title: J Hered. Volume: 110 Issue: 2 Pages: 183-193
  • DOI: 10.1093/jhered/esy060
  • NAID: 130007485879
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Genetic characterization of Indonesian Pesisir cattle using mitochondrial DNA and Y-chromosomal haplotypes and loci associated with economical traits and coat color. (2018)
  • Author(s): Putra, D. E, Paul, R. C., Le, T. N. A., Okuda, Y., Yurnalis, Ibi, T., Kunieda, T.
  • Journal Title: J. Anim. Genet. Volume: 46 Pages: 17-23
  • NAID: 130006606820
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Genetic characterization of Vietnamese Yellow cattle using mitochondrial DNA and Y-chromosomal haplotypes and genes associated with economical traits. (2018)
  • Author(s): Le TNA, Vu HV, Okuda Y, Duong HT, Nguyen TB, Nguyen VH, Le PD, Kunieda T.
  • Journal Title: Anim Sci J. Volume: 89 Issue: 12 Pages: 1641-1647
  • DOI: 10.1111/asj.13099
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Coat color variation and allelic frequency of the genes associated with body composition and locomotion traits in Kazakhstan native horse. (2017)
  • Author(s): Kunieda T, Ezoe H, Okuda Y, Mannen H, Takahashi Y, Nomura K, Yamagata T, Yamamoto Y, Tsunoda, K, Bakhtin M, Kazymbet P, Alykhan M, Suleimenov MZ, Safronova O, Nishibori M.
  • Journal Title: Report of Society for Researches on Native Livestock, Volume: 28 Pages: 53-63
  • Int'l Joint Research
• [Presentation] Genetic characterization of Kazakhstan Kushum horse by mtDNA and y chromosome haplotypes and genes associated with coat color, body composition, and physical performance. (2019)
  • Author(s): Nguyen Ba Trung, Le Nu Anh Thu, Ripon C. Paul, Phuong T. K. Pham, Yu. Okuda, Kushaliye. Kaissar, Akhmedenov Kazhmurat, Sarsenova Bibigul, Meirat Bakhtin, Polat Kazymbet, Suleimenov Zh Maratbek, Alykhan Meldelokov, Masahide Nishibori, Tetsuo Kunieda.
  • Organizer: 20th Annual meeting of Japanese Society of Animal Genetics and breeding
• [Presentation] Genetic Characterization of Japanese Native Horses (2018)
  • Author(s): Chandra, P.R., Okuda, Y., Kawamoto, Y., Nozawa, K. Kunieda, T.
  • Organizer: 18th Asian Australasian Animal Production Congress
  • Int'l Joint Research
• [Presentation] Genetic Characterization of Kushum Horse by mtDNA and Genes Associated with Coat Color, Body Composition, and Physical Performance. (2018)
  • Author(s): Nguyen, B.T., Thu, L.N.A., Chandra, P.R., Okuda, Y., Kushaliyev Kaissar, J., Akhmedenov Kazhmurat, M., Bibigul, S., Bakhtin, M., Kazymbet, P., Maratbek, S.Zh., Meldelokov, A., Nishibori, M. & Kunieda, T.
  • Organizer: 18th Asian Australasian Animal Production Congress
• [Presentation] Genetic Characteristics and Diversity of Dairy Cattle Population in Fiji Revealed by Distribution of mtDNA Haplotypes. (2018)
  • Author(s): Tabuyaqona, J., Chandra, R.P., Le, N.A.T. Kunieda, T.
  • Organizer: 18th Asian Australasian Animal Production Congress
• [Presentation] Genetic Characterization of Vietnamese Yellow Cattle using Mitochondrial DNA and Y-chromosomal Haplotypes and Loci Associated with Economical Traits. (2018)
  • Author(s): Le, N.A.T., Vu, V.H., Okuda, Y., Nguyen, B.T., Le, D.P., Nguyen, H.V. Kunieda, T.
  • Organizer: 18th Asian Australasian Animal Production Congress
• [Presentation] Origin and genetic diseases of Japanese Black cattle (2018)
  • Author(s): Kunieda, T.
  • Organizer: The 30th World Buiatric Congress,
  • Invited
• [Presentation] Genetic diversity and hereditary disorders of Japanese beef cattle (2018)
  • Author(s): Kunieda, T.
  • Organizer: The 1st International Conference on Animal Production for Food Sustainability.
  • Invited
• [Presentation] Genetic Characterization of Japanese native horse (2018)
  • Author(s): Paul Ripon Chandra・奥田 ゆう・川本 芳・野澤 謙・国枝 哲夫
  • Organizer: 日本畜産学会第１２４回大会
• [Presentation] Coat color variation and allelic frequency of the genes associated with body composition and locomotion traits in Kazakhstan native horse. (2017)
  • Author(s): Y. Okuda, H. Moe, K. Moe, B. Kounnavongsa, S. Keonouchanh, B. Bouahom, T. Shimogiri, H. Mannen, M. Kanemaki, Y. Yamamoto, T. Kunieda
  • Organizer: 36th International Society for Animal Genetics Conference
  • Int'l Joint Research
• [Presentation] Genetic characteristics of Kazakhstan native horses. (2017)
  • Author(s): H. Ezoe, Y. Okuda, M. Nishibori, H. Mannen, Y. Takahashi, K. Nomura, T. Yamagata, Y. Yamamoto, K. Tsunoda, M. Bakhtin, P. Kazymbet, M. Alykhan, M. Suleimenov, O. Safronova, T. Kunieda1
  • Organizer: 36th International Society for Animal Genetics Conference
  • Int'l Joint Research