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Pathological investigation and analysis of transboundary swine disease threaten to Japan in south Asia

Research Project

Project/Area Number 17H04639
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Veterinary medical science
Research InstitutionUniversity of Miyazaki

Principal Investigator

Yamaguchi Ryoji  宮崎大学, 農学部, 特別教授 (90150169)

Co-Investigator(Kenkyū-buntansha) 乗峰 潤三  宮崎大学, 農学部, 教授 (30627667)
菱川 善隆  宮崎大学, 医学部, 教授 (60304276)
Project Period (FY) 2017-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2021: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2020: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Keywords病理学的研究 / 豚熱CSF / アフリカ豚熱ASF / 豚 / 越境感染症 / 国際共同研究 / 東南アジア / ベトナム / 豚流行性下痢症PED / 豚の越境感染症 / アフリカ豚熱(ASF) / 豚熱(CSF) / 豚下痢症ウイルス感染症(PED) / 豚コレラ(豚熱) / CSF / アフリカ豚コレラ(アフリカ豚熱) / ASF / 病理 / 免疫細胞化学 / アフリカ豚コレラ / 豚コレラ / 免疫組織化学的染色 / 遺伝子解析 / 豚コレラ CSF / 病理学的解析 / ウイルス抗原分布 / PED / 豚流行性下痢(PED) / 抗体作成 / 豚の海外疾病 / 病理学 / 畜産学 / 国際貢献 / ウイルス性疾患
Outline of Final Research Achievements

We conducted pathological studies of transboundary infectious diseases in pigs, which poses a threat to Japan from Southeast Asia. Classical swine fever (CSF), African swine fever (ASF), and highly pathogenic PRRS have similarities of many macroscopic lesions including hemorrhage in lymph node and clinical signs. By pathological analysis, the process of lesion formation, the cells to be infected, and the comparison with the detection of viral genes were clearly different, and the difference in pathogenesis was clarified. It shown that viruses mainly infect lymphocytes, epithelial cells, vascular endothelium, and nerve cells in CSF, systemic macrophages and a few of hepatocytes and renal cells in ASF, and macrophages in lymphatic tissues and lungs in highly pathogenic PRRS in filed cases. This was an important finding for diagnosis assessment in the event of a new transboundary infectious disease in pigs to Japan.

Academic Significance and Societal Importance of the Research Achievements

産業動物感染症の病理学的研究は、遺伝子解析の機器が発達している中で、診断、病態解析の技術の中で重要となっている。特に生体内でのウイルスなどの病原体の分布と生体反応は病理以外では研究できない。問題点は豚の重要疾病の病理学的研究は抗体の入手の困難さ、病理学者が少ないこと、発生地の東南アジアの病理学的標本作製の技術的問題と材料を採取する国際共同研究としてのカウンターパートとの協力関係など多かった。今回はこれをすべてクリアする状況ができ病理学的研究が可能となり、豚の越境感染症の病理学的研究の結果、流行地での豚の疾患、豚熱、アフリカ豚熱、高病原性PRRS等の詳細な病気の形成過程が明確となった。

Report

(6 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (21 results)

All 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (6 results) Journal Article (12 results) (of which Int'l Joint Research: 9 results,  Peer Reviewed: 12 results,  Open Access: 4 results) Presentation (3 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Int'l Joint Research] Vietnam National University of Agri(ベトナム)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] University of Veterinary Science(ミャンマー)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Chulalongkorn University(タイ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Vietnam university of Agriculture(ベトナム)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Vietnam University of Agriculture(ベトナム)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] VietnumNationalUniversityofAgriculture(ベトナム)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Natural Mycoplasma bovis infection associated with epithelioid granulomatous caseonecrotic bronchiolitis at a progressed disease stage in calves2022

    • Author(s)
      Mathurot Suwanruengsri,Ryoko Uemura,Uda Zahli Izzati,Takuya Kanda,Naoyuki Fuke,Masahiro Yasuda,Takuya Hirai,Ryoji Yamaguchi
    • Journal Title

      Thai Journal of Veterinary Medicine

      Volume: 52 Pages: 33-44

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Pathological lesions and presence of viral antigens in four surviving pigs in African swine fever outbreak farms in Vietnam2021

    • Author(s)
      PORNTHUMMAWAT Apisit、TRUONG Quang Lam、HOA Nguyen Thi、LAN Nguyen Thi、IZZATI Uda Zahli、SUWANRUENGSRI Mathurot、NUEANGPHUET Phawut、HIRAI Takuya、YAMAGUCHI Ryoji
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 83 Issue: 11 Pages: 1653-1660

    • DOI

      10.1292/jvms.21-0409

    • NAID

      130008109059

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A persistent epidemic of porcine epidemic diarrhoea virus infection by serological survey of commercial pig farms in northern Vietnam2021

    • Author(s)
      Myint Ohnmar、Hoa Nguyen Thi、Fuke Naoyuki、Pornthummawat Apisit、Lan Nguyen Thi、Hirai Takuya、Yoshida Ayako、Yamaguchi Ryoji
    • Journal Title

      BMC Veterinary Research

      Volume: 17 Issue: 1

    • DOI

      10.1186/s12917-021-02941-7

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Neutrophil and M2-polarized Macrophage Infiltration, Expression of IL-8 and Apoptosis in Mycoplasma hyopneumoniae Pneumonia in Swine2021

    • Author(s)
      Nueangphuet Phawut、Suwanruengsri Mathurot、Fuke Naoyuki、Uemura Ryoko、Hirai Takuya、Yamaguchi Ryoji
    • Journal Title

      Journal of Comparative Pathology

      Volume: 189 Pages: 31-44

    • DOI

      10.1016/j.jcpa.2021.09.004

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Production of granulomas in <i>Mycoplasma bovis</i> infection associated with meningitis-meningoencephalitis, endocarditis, and pneumonia in cattle2021

    • Author(s)
      Suwanruengsri Mathurot、Uemura Ryoko、Kanda Takuya、Fuke Naoyuki、Nueangphuet Phawut、Pornthummawat Apisit、Yasuda Masahiro、Hirai Takuya、Yamaguchi Ryoji
    • Journal Title

      Journal of Veterinary Diagnostic Investigation

      Volume: 34 Issue: 1 Pages: 68-76

    • DOI

      10.1177/10406387211053254

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Distribution of Pseudorabies Virus Antigen in Hunting Dogs with Concurrent Paragonimus westermani Infection2021

    • Author(s)
      Izzati Uda Z.、Kaneko Yasuyuki、Kaneko Chiho、Yoshida Ayako、Suwanruengsri Mathurot、Okabayashi Tamaki、Hirai Takuya、Yamaguchi Ryoji
    • Journal Title

      Journal of Comparative Pathology

      Volume: 188 Pages: 44-51

    • DOI

      10.1016/j.jcpa.2021.08.004

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Pseudorabies virus infection in hunting dogs in Oita, Japan: Report from a prefecture free from Aujeszky’s disease in domestic pigs2021

    • Author(s)
      KANEKO Chiho、KANEKO Yasuyuki、SUDARYATMA Putu Eka、MEKATA Hirohisa、KIRINO Yumi、YAMAGUCHI Ryoji、OKABAYASHI Tamaki
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 83 Issue: 4 Pages: 680-684

    • DOI

      10.1292/jvms.20-0450

    • NAID

      130008023282

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Pathology of the outbreak of subgenotype 2.5 classical swine fever virus in northern Vietnam2020

    • Author(s)
      Izzati Uda Zahli、Hoa Nguyen Thi、Lan Nguyen Thi、Diep Nguyen Van、Fuke Naoyuki、Hirai Takuya、Yamaguchi Ryoji
    • Journal Title

      Veterinary Medicine and Science

      Volume: 7 Issue: 1 Pages: 164-174

    • DOI

      10.1002/vms3.339

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pathological investigation and viral antigen distribution of emerging African swine fever in Vietnam2020

    • Author(s)
      Izzati Uda Zahli、Inanaga Minori、Hoa Nguyen Thi、Nueangphuet Phawut、Myint Ohnmar、Truong Quang Lam、Lan Nguyen Thi、Norimine Junzo、Hirai Takuya、Yamaguchi Ryoji
    • Journal Title

      Transboundary and Emerging Diseases

      Volume: 67 Issue: 4 Pages: 2589-2601

    • DOI

      10.1111/tbed.13851

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] New tropisms of porcine epidemic diarrhoea virus (PEDV) in pigs naturally coinfected by variants bearing large deletions in the spike (S) protein and PEDVs possessing an intact S protein2020

    • Author(s)
      Van Diep Nguyen、Choijookhuu Narantsog、Fuke Naoyuki、Myint Ohnmar、Izzati Uda Zahli、Suwanruengsri Mathurot、Hishikawa Yoshitaka、Yamaguchi Ryoji
    • Journal Title

      Transboundary and Emerging Diseases

      Volume: 67 Issue: 6 Pages: 2589-2601

    • DOI

      10.1111/tbed.13607

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Development of indirect enzyme-linked immunosorbent assay for detection of porcine epidemic diarrhea virus specific antibodies (IgG) in serum of naturally infected pigs2019

    • Author(s)
      Myint Ohnmar、Yoshida Ayako、Sekiguchi Satoshi、Van Diep Nguyen、Fuke Naoyuki、Izzati Uda Zahli、Hirai Takuya、Yamaguchi Ryoji
    • Journal Title

      BMC Veterinary Research

      Volume: 15 Issue: 1 Pages: 0-0

    • DOI

      10.1186/s12917-019-2123-2

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Molecular characterization of US-like and Asian non-S INDEL strains of porcine epidemic diarrhea virus (PEDV) that circulated in Japan during 2013-2016 and PEDVs collected from recurrent outbreaks.2018

    • Author(s)
      Van Diep N, Sueyoshi M, Norimine J, Hirai T, Myint O, Teh APP, Izzati UZ, Fuke N, Yamaguchi R
    • Journal Title

      BMC Veterinary Research

      Volume: 14 Issue: 1 Pages: 96-107

    • DOI

      10.1186/s12917-018-1409-0

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Pathological study of subgenotype 2.5 classical swine fever virus endemic in Northern Vietnam2019

    • Author(s)
      Uda Zahli Izzati, Nguyen Thi Hoa, Nguyen Thi Lan, Nguyen Van Diep, Takuya Hirai, Ryoji Yamaguchi
    • Organizer
      第162回日本獣医学会学術集会、つくば国際会議場
    • Related Report
      2019 Annual Research Report
  • [Presentation] Pathological studies of possible transboundary diseases in pigs2019

    • Author(s)
      Ryoji YAMAGCUHI
    • Organizer
      アジア獣医病理学会、ベトナム
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Development of Indirect Enzyme-linked Immunosorbent Assay for Detection of Porcine Epidemic Diarrhea Virus Specific Antibodies (IgG) in Serum of Natural Infected Pigs2019

    • Author(s)
      Ohnmar Myint, Ayako Yoshida, Satoshi Sekiguchi , Nguyen Van Diep, Naoyuki Fuke, Uda Zahli Izzati, Takuya Hirai, Ryoji Yamaguchi*
    • Organizer
      第162回日本獣医学会学術集会、つくば国際会議場
    • Related Report
      2019 Annual Research Report

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Published: 2017-04-28   Modified: 2023-01-30  

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