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Establishment of a diagnostic method for asymptomatic infected people using a proprietary device in malaria endemic areas

Research Project

Project/Area Number 17H04650
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Parasitology (including sanitary zoology)
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Hashimoto Muneaki  国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (30407308)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2020: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Keywordsマラリア / 検査 / 診断機器開発 / アフリカ / 診断 / 機器開発 / 熱帯熱マラリア / 無症状マラリア / 診断機器 / 薬剤耐性 / 種の同定 / パラシテミア / ケニア / 感染症 / 診断法
Outline of Final Research Achievements

The purpose of this study was to develop a next-generation diagnostic device for eradicating malaria by using an automatic malaria diagnostic device originally developed by the applicants for asymptomatic infected people in malaria endemic areas and comparing it with the current method. After improving the device, it became clear that the device could be used for diagnosis with high accuracy. Specifically, using the nested PCR method as a reference method, the sensitivity and specificity of this device were 100% and 92.8%, respectively. In addition, for 53 malarial parasite-positive specimens, the parasitemia value calculated by this device was compared with the value calculated by microscopy, revealing the high quantification of this device.

Academic Significance and Societal Importance of the Research Achievements

マラリア流行地では無症候感染者(繰返しの感染により部分的な免疫の獲得し、低感染状態の感染者)が原虫のドナーになっており、マラリア撲滅のためには無症候感染者の治療による伝播阻止が鍵になる。このような背景から、流行地でのフィールドワークで無症候感染者にも使用可能な迅速かつ易操作な高感度マラリア診断デバイスの開発が望まれている。申請者らはこれらを可能にし得るデバイスを独自に開発した。本研究では罹患率が特に高いマラリア流行地であるケニアにおいて無症候感染者を対象に本デバイスを用いた原虫の高感度検出を可能にし、マラリア撲滅に大きく貢献すると期待される。

Report

(5 results)
  • 2022 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (16 results)

All 2020 2019 2018 2017

All Journal Article (11 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 11 results,  Open Access: 8 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Quantitative Detection of Plasmodium falciparum Using, LUNA-FL, A Fluorescent Cell Counter2020

    • Author(s)
      Hashimoto Muneaki、Yokota Kazumichi、Kajimoto Kazuaki、Matsumoto Musashi、Tatsumi Atsuro、Yamamoto Kenichi、Hyodo Tomonori、Matsushita Kiichiro、Minakawa Noboru、Mita Toshihiro、Oka Hiroaki、Kataoka Masatoshi
    • Journal Title

      Microorganisms

      Volume: 8 Issue: 9 Pages: 1356-1356

    • DOI

      10.3390/microorganisms8091356

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Highly Sensitive and Rapid Quantitative Detection of Plasmodium falciparum Using an Image Cytometer2020

    • Author(s)
      Hashimoto Muneaki、Yokota Kazumichi、Kajimoto Kazuaki、Matsumoto Musashi、Tatsumi Atsuro、Nakajima Yoshihiro、Mita Toshihiro、Minakawa Noboru、Oka Hiroaki、Kataoka Masatoshi
    • Journal Title

      Microorganisms

      Volume: 8 Issue: 11 Pages: 1769-1769

    • DOI

      10.3390/microorganisms8111769

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a quantitative, portable, and automated fluorescent blue-ray device-based malaria diagnostic equipment with an on-disc SiO2 nanofiber filter2020

    • Author(s)
      Yamamoto Takeki、Hashimoto Muneaki、Nagatomi Kenji、Nogami Takahiro、Sofue Yasuyuki、Hayashi Takuya、Ido Yusuke、Yatsushiro Shouki、Abe Kaori、Kajimoto Kazuaki、Tamari Noriko、Awuor Beatrice、Sonye George、Kongere James、Munga Stephen、Ohashi Jun、Oka Hiroaki、Minakawa Noboru、Kataoka Masatoshi、Mita Toshihiro
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 6585-6585

    • DOI

      10.1038/s41598-020-63615-2

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Nucleic acid purification from dried blood spot on FTA Elute Card provides template for polymerase chain reaction for highly sensitive Plasmodium detection2019

    • Author(s)
      Hashimoto Muneaki、Bando Mika、Kido Jun-ichi、Yokota Kazumichi、Mita Toshihiro、Kajimoto Kazuaki、Kataoka Masatoshi
    • Journal Title

      Parasitology International

      Volume: 73 Pages: 101941-101941

    • DOI

      10.1016/j.parint.2019.101941

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Small-scale culture of Plasmodium falciparum using μ-Slide Angiogenesis followed by automatic infection rate counting to assess drug effects2019

    • Author(s)
      Hashimoto Muneaki、Ido Yusuke、Tanaka Masato、Yokota Kazumichi、Kajimoto Kazuaki、Kataoka Masatoshi
    • Journal Title

      Parasitology International

      Volume: 69 Pages: 54-58

    • DOI

      10.1016/j.parint.2018.11.005

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a highly sensitive, quantitative, and rapid detection system for Plasmodium falciparum-infected red blood cells using a fluorescent blue-ray optical system2019

    • Author(s)
      Yamamoto Takeki、Yatsushiro Shouki、Hashimoto Muneaki、Kajimoto Kazuaki、Ido Yusuke、Abe Kaori、Sofue Yasuyuki、Nogami Takahiro、Hayashi Takuya、Nagatomi Kenji、Minakawa Noboru、Oka Hiroaki、Mita Toshihiro、Kataoka Masatoshi
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 132 Pages: 375-381

    • DOI

      10.1016/j.bios.2019.02.064

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Loop-Mediated Isothermal Amplification in Microchambers on a Cell Microarray Chip for Identification of Plasmodium Species2019

    • Author(s)
      Ido Yusuke、Hashimoto Muneaki、Yatsushiro Shouki、Tanaka Masato、Yokota Kazumichi、Kajimoto Kazuaki、Kataoka Masatoshi
    • Journal Title

      Journal of Parasitology

      Volume: 105 Issue: 1 Pages: 69-69

    • DOI

      10.1645/18-107

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PSEUDO iRBC BEADS AS POSITIVE CONTROL FOR CELL MICROARRAY CHIP-BASED DETECTION OF PLASMODIUM-INFECTED RBCs2018

    • Author(s)
      Hashimoto Muneaki、Numata Masahiko、Yatsushiro Shouki、Ido Yusuke、Tanaka Masato、Kajimoto Kazuaki、Kataoka Masatoshi
    • Journal Title

      Journal of Parasitology

      Volume: in press Issue: 3 Pages: 283-288

    • DOI

      10.1645/17-144

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In situ loop-mediated isothermal amplification (LAMP) for identification of Plasmodium species in wide-range thin blood smears2018

    • Author(s)
      Hashimoto Muneaki、Sakamoto Hirokazu、Ido Yusuke、Tanaka Masato、Yatsushiro Shouki、Kajimoto Kazuaki、Kataoka Masatoshi
    • Journal Title

      Malaria Journal

      Volume: 17 Issue: 1 Pages: 235-235

    • DOI

      10.1186/s12936-018-2381-7

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hydrophilic-treated plastic plates for wide-range analysis of Giemsa-stained red blood cells and automated Plasmodium infection rate counting2017

    • Author(s)
      Hashimoto Muneaki、Yatsushiro Shouki、Yamamura Shohei、Tanaka Masato、Sakamoto Hirokazu、Ido Yusuke、Kajimoto Kazuaki、Bando Mika、Kido Jun-ichi、Kataoka Masatoshi
    • Journal Title

      Malaria Journal

      Volume: 16 Issue: 1 Pages: 321-321

    • DOI

      10.1186/s12936-017-1975-9

    • NAID

      120006767282

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sensitivity of Loop-Mediated Isothermal Amplification Increases 100-Fold after TritonTM?X-100 Treatment of?Plasmodium-Infected Erythrocytes2017

    • Author(s)
      Hashimoto Muneaki
    • Journal Title

      SOJ Microbiology & Infectious Diseases

      Volume: 5 Issue: 4 Pages: 1-2

    • DOI

      10.15226/sojmid/5/4/00177

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] In situ loop-mediated isothermal amplification (LAMP) for identification of Plasmodium species in wide-range thin blood smears2018

    • Author(s)
      橋本 宗明、坂本 寛和、井戸 佑介、田中 正人、八代 聖基、梶本 和昭、片岡 正俊
    • Organizer
      日本寄生虫学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The malaria cell disc system: Development of a portable and highly sensitive CDplayer-like automatic malaria diagnosis device2018

    • Author(s)
      Muneaki Hashimoto
    • Organizer
      日本熱帯医学会大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 赤血球を単層配列させる技術をマラリア診断・研究に応用する2018

    • Author(s)
      橋本宗明
    • Organizer
      分子寄生虫学ワークショップ・分子寄生虫・マラリア研究フォーラム
    • Related Report
      2017 Annual Research Report
  • [Presentation] Preparation of a uniform monolayer of Giemsa-stained red blood cells on hydrophilic-treated plastic plates for malaria diagnosis2017

    • Author(s)
      Muneaki Hashimoto, Shohei Yamamura, Hirokazu Sakamoto, Masato Tanaka, Shouki Yatsushiro, Kazuaki Kajimoto, Masatoshi Kataoka
    • Organizer
      日本寄生虫学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Preparation of a uniform monolayer of Giemsa-stained red blood cells on hydrophilic-treated plastic plates for malaria diagnosis2017

    • Author(s)
      Muneaki Hashimoto, Shouki Yatsushiro, Shohei Yamamura, Masato Tanaka, Hirokazu Sakamoto, Yusuke Ido, Kazuaki Kajimoto, Mika Bando, Jun-ichi Kido, Masatoshi Kataoka
    • Organizer
      AMERICAN SOCIETY OF TROPICAL MEDICINE and HYGIENE
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2024-01-30  

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