Epidemiological surveys of tick-borne pathogens in Kenya

• Project/Area Number: 17H04661
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Hygiene and public health
• Research Institution: Yamaguchi University (2019-2021); Nagasaki University (2017-2018)
• Principal Investigator: HAYASAKA DAISUKE 山口大学, 共同獣医学部, 教授 (10346926)
• Co-Investigator(Kenkyū-buntansha): 井上 真吾 長崎大学, 熱帯医学研究所, 准教授 (00346925) ; 高野 愛 山口大学, 共同獣医学部, 准教授 (90700055)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2018: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: ケニア / マダニ / ウイルス / マダニ媒介病原体 / マダニ媒介ウイルス / リケッチア / 血清疫学調査 / マダニ媒介細菌 / 細菌 / ボレリア / 病原体 / 人獣共通感染症

Outline of Final Research Achievements

In this study, we attempted to detect tick-borne pathogens from ticks in Kenya. RT-PCR results showed that Orthonairovirus specific bands were detected from Amblyomma and Rhipicephalus spp. Further analyses were not performed due to the influence of COVID-19, but we could establish a research base for investigation of tick-borne diseases in Kenya.

Academic Significance and Societal Importance of the Research Achievements

マダニは、ヒトや動物に様々な病原体を媒介し、日本では日本紅斑熱や重症熱性血小板減少症候群など致死性の感染症がよく知られている。一方、アフリカにもマダニ媒介感染症の発生はあるが、どのような病原体が存在しているかについては不明な点が多い。本研究は、ケニアにおいてマダニ媒介病原体の実態を調べる研究で、現地での感染症の予防に有用な情報を提供する意義が大きい。

Research Products

• [Int'l Joint Research] Kenya Medical Research Institute(ケニア)
• [Int'l Joint Research] Kenya Medical Research Institute(ケニア)
• [Int'l Joint Research] ケニア国営医学研究機関(ケニア)
• [Int'l Joint Research] Kenya Medical Research Institute(Kenya)
• [Journal Article] Seroepidemiological surveys of tick-borne encephalitis virus and novel tick-borne viruses in wild boar in Nagasaki, Japan (2022)
  • Author(s): Luvai Elizabeth Ajema Chebichi、Uchida Leo、Tun Mya Myat Ngwe、Inoue Shingo、Weiyin Hu、Shimoda Hiroshi、Morita Kouichi、Hayasaka Daisuke
  • Journal Title: Ticks and Tick-borne Diseases Volume: 13 Issue: 1 Pages: 101860-101860
  • DOI: 10.1016/j.ttbdis.2021.101860
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Epidemiological study of Kabuto Mountain virus, a novel uukuvirus, in Japan (2022)
  • Author(s): TRAN Ngo T.B.、SHIMODA Hiroshi、MIZUNO Junko、ISHIJIMA Keita、YONEMITSU Kenzo、MINAMI Shohei、Supriyono、KURODA Yudai、TATEMOTO Kango、MENDOZA Milagros V.、TAKANO Ai、MUTO Masahiko、ISAWA Haruhiko、SAWABE Kyoko、HAYASAKA Daisuke、MAEDA Ken
  • Journal Title: Journal of Veterinary Medical Science Volume: 84 Issue: 1 Pages: 82-89
  • DOI: 10.1292/jvms.21-0577
  • NAID: 130008139950
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Zoonotic Infection with Oz Virus, a Novel Thogotovirus (2022)
  • Author(s): Tran Ngo T.B.、Shimoda Hiroshi、Ishijima Keita、Yonemitsu Kenzo、Minami Shohei、Kuroda Yudai、Tatemoto Kango、Mendoza Milagros V.、Kuwata Ryusei、Takano Ai、Muto Masahiko、Sawabe Kyoko、Isawa Haruhiko、Hayasaka Daisuke、Maeda Ken、Supriyono
  • Journal Title: Emerging Infectious Diseases Volume: 28 Issue: 2 Pages: 436-439
  • DOI: 10.3201/eid2802.211270
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Severe Fever with Thrombocytopenia Syndrome in Cats and Its Prevalence among Veterinarian Staff Members in Nagasaki, Japan (2021)
  • Author(s): Ando Tsuyoshi、Nabeshima Takeshi、Inoue Shingo、Tun Mya Myat Ngwe、Obata Miho、Hu Weiyin、Shimoda Hiroshi、Kurihara Shintaro、Izumikawa Koichi、Morita Kouichi、Hayasaka Daisuke
  • Journal Title: Viruses Volume: 13 Issue: 6 Pages: 1142-1142
  • DOI: 10.3390/v13061142
  • Peer Reviewed / Open Access
• [Journal Article] Pathogenetic Potential Relating to Metabolic Activity in a Mouse Model of Infection with the Chikungunya Virus East/Central/South African Genotype (2020)
  • Author(s): Ngwe Tun Mya Myat、Muthugala Rohitha、Kyaw Kyaw Aung、Shimada Satoshi、Morita Kouichi、Hayasaka Daisuke
  • Journal Title: Viruses Volume: 12 Issue: 2 Pages: 169-169
  • DOI: 10.3390/v12020169
  • NAID: 120006988301
  • Peer Reviewed / Open Access
• [Journal Article] Seroprevalence of yellow fever, dengue, West Nile and chikungunya viruses in children in Teso South Sub-County, Western Kenya (2020)
  • Author(s): Inziani Mary、Adungo Ferdinard、Awando Janet、Kihoro Richelle、Inoue Shingo、Morita Kouichi、Obimbo Elizabeth、Onyango Francis、Mwau Matilu
  • Journal Title: International Journal of Infectious Diseases Volume: 91 Pages: 104-110
  • DOI: 10.1016/j.ijid.2019.11.004
  • Peer Reviewed / Open Access
• [Journal Article] Detection and isolation of tick‐borne bacteria (Anaplasma spp., Rickettsia spp., and Borrelia spp.) in Amblyomma varanense ticks on lizard (Varanus salvator) (2019)
  • Author(s): Supriyono、Takano Ai、Kuwata Ryusei、Shimoda Hiroshi、Hadi Upik K.、Setiyono Agus、Agungpriyono Srihadi、Maeda Ken
  • Journal Title: Microbiology and Immunology Volume: 63 Issue: 8 Pages: 328-333
  • DOI: 10.1111/1348-0421.12721
  • NAID: 40021982758
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Detection of a novel tick-borne flavivirus and its serological surveillance. (2019)
  • Author(s): Shimoda H, Hayasaka D, Yoshii K, Yokoyama M, Suzuki K, Kodera Y, Takeda T, Mizuno J, Noguchi K, Yonemitsu K, Minami S, Kuwata R, Takano A, Maeda K.
  • Journal Title: Ticks Tick Borne Dis. Volume: - Issue: 4 Pages: 742-748
  • DOI: 10.1016/j.ttbdis.2019.03.006
  • Peer Reviewed / Open Access
• [Journal Article] Survey of tick-borne zoonotic viruses in wild deer in Hokkaido, Japan (2018)
  • Author(s): Leo Uchida, Daisuke Hayasaka, Mya Myat Ngwe Tun, Kouichi Morita, Yasukazu Muramatsu, Katsuro Hagiwara
  • Journal Title: Journal of Veterinary Medical Science Volume: 80 Issue: 6 Pages: 985-988
  • DOI: 10.1292/jvms.18-0017
  • NAID: 130007412692
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] A Simple Mechanism Based on Amino Acid Substitutions is not a Critical Determinant of High Mortality of Japanese Encephalitis Virus Infection in Mice (2018)
  • Author(s): Takamatsu Yuki、Uchida Leo、Raekiansyah Muhareva、Luz Mark、Morita Kouichi、Hayasaka Daisuke
  • Journal Title: Viruses Volume: 10 Issue: 2 Pages: 62-62
  • DOI: 10.3390/v10020062
  • NAID: 120006798166
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The genotypes of Orientia tsutsugamushi, identified in scrub typhus patients in northern Vietnam (2017)
  • Author(s): Nguyen Hang L.K.、Pham Hang T.T.、Nguyen Tinh V.、Hoang Phuong VM、Le Mai T.Q.、Takemura Taichiro、Hasebe Futoshi、Hayasaka Daisuke、Yamada Akio、Hotta Kozue
  • Journal Title: Transactions of The Royal Society of Tropical Medicine and Hygiene Volume: 111 Issue: 3 Pages: 137-139
  • DOI: 10.1093/trstmh/trx022
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Tick surveillance for Borrelia miyamotoi and phylogenetic analysis of isolates in Mongolia and Japan (2017)
  • Author(s): Iwabu-Itoh Yukie、Bazartseren Boldbaatar、Naranbaatar Oyunnomin、Yondonjamts Enkhmandakh、Furuno Kiwa、Lee Kyunglee、Sato Kozue、Kawabata Hiroki、Takada Nobuhiro、Andoh Masako、Kajita Hiroko、Oikawa Yosaburo、Nakao Minoru、Ohnishi Makoto、Watarai Masahisa、Shimoda Hiroshi、Maeda Ken、Takano Ai
  • Journal Title: Ticks and Tick-borne Diseases Volume: 8 Issue: 6 Pages: 850-857
  • DOI: 10.1016/j.ttbdis.2017.06.011
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 長崎県のイノシシを対象としたマダニ媒介ウイルスの血清疫学調査 (2021)
  • Author(s): Elizabeth A.C. Luvai、内田玲麻、Mya Myat Ngwe Tun、井上真吾、胡 蔚 殷、下田宙、森田公一、早坂大輔
  • Organizer: 第55回日本脳炎ウイルス生態学研究会
• [Presentation] イノシシを対象としたマダニ媒介ウイルスの血清疫学調査 (2021)
  • Author(s): Elizabeth Luvai, 内田 玲麻, Mya Myat Ngwe Tun, 胡 蔚殷, 下田 宙, 森田 公一, 早坂 大輔
  • Organizer: 第164回日本獣医学会学術集会
• [Presentation] イノシシを対象としたマダニ媒介ウイルスの血清疫学調査 (2021)
  • Author(s): Elizabeth Luvai, 内田 玲麻, Mya Myat Ngwe Tun, 胡 蔚殷, 下田 宙, 森田 公一, 早坂 大輔
  • Organizer: 第３回 SFTS 研究会 ・学術集会
• [Presentation] 動物におけるSFTSの現状 (2019)
  • Author(s): 早坂 大輔
  • Organizer: 市民公開シンポジウム SFTS発見の地より～SFTSの現在～
  • Invited
• [Presentation] 長崎県内のネコにおけるSFTSウイルス感染の疫学調査 (2019)
  • Author(s): Epidemiological survey of SFTS virus infections in cats in Nagasakai
  • Organizer: 第60回日本熱帯医学会大会
• [Presentation] Sero-epidemiological surveillance of Tick-borne viruses in sentinel animals in Nagasaki, Japan (2019)
  • Author(s): Elizabeth Luvai，Mya Myat Ngwe Tun，Shingo Inoue，Kouichi Morita，Daisuke Hayasaka
  • Organizer: 第60回日本熱帯医学会大会
• [Presentation] 長崎県内のネコにおけるSFTSV感染の調査 (2019)
  • Author(s): 安藤 豪、鍋島 武、井上 真吾、Mya Myat Ngwe Tun、森田 公一、早坂 大輔
  • Organizer: 第2回日本SFTS研究会学術総会
• [Presentation] モンゴルにおける節足動物媒介ウイルス感染症の調査 (2019)
  • Author(s): 下田宙、水野純子、野口慧多、黒田雄大、立本完吾、メンドーサ ミラグロス、鍬田龍星、高野愛、バザートセレン ボールドバートル、前田 健
  • Organizer: 第67回日本ウイルス学会学術集会
• [Presentation] 硬ダニ媒介性回帰熱群ボレリア菌のゲノム解析 (2019)
  • Author(s): 中尾蘭那、笠間健太郎、小椋義俊、林哲也、川端寛樹、下田宙、前田健、高野愛
  • Organizer: 第162回日本獣医学会学術集会
• [Presentation] ダイレクトシークエンス法によるRickettsia sp. Lonとマダニ宿主の多様性と共進化の解明 (2019)
  • Author(s): 笠間健太郎、後藤恭宏、小椋義俊、山本正悟、藤田博己、高野 愛、安藤秀二、林哲也
  • Organizer: 第92回日本細菌学会総会
• [Presentation] 長崎県のネコ、イヌにおけるSFTSの調査 (2018)
  • Author(s): 安藤豪、井上真吾、Mya Myat Ngwe Tun、森田公一、早坂大輔
  • Organizer: 第25回リケッチア研究会 第11回日本リケッチア臨床研究会
• [Presentation] 長崎県内のネコ及びイヌにおけるSFTSV感染の調査 (2018)
  • Author(s): 安藤豪、井上真吾、Mya Myat Ngwe Tun、森田公一、早坂大輔
  • Organizer: 第1回SFTS研究会・学術集会
• [Presentation] 長崎県におけるトフラウイルスの調査 (2018)
  • Author(s): 早坂大輔、安藤豪、嶋田聡、Luz Mark Anthony、Mya Myat Ngwe Tun、森田公一
  • Organizer: 第26回ダニと疾患のインターフェースに関するセミナー
• [Presentation] Epidemiological survey of tick-borne viruses in Nagasaki, Japan (2018)
  • Author(s): Daisuke Hayasaka, Yu Fuxun, Leo Uchida, Satoshi Shimada, Luz Mark Anthony, Mya Myat Ngwe Tun, Kouichi Morita
  • Organizer: 12th China Japan Virology Conference
  • Int'l Joint Research
• [Presentation] ボレリア感染症研究の現状 (2018)
  • Author(s): 高野愛、伊藤幸枝、DeMar Taylor、川端寛樹、前田健
  • Organizer: 第91回日本細菌学会総会
• [Presentation] 長崎県のイノシシにおけるダニ媒介性ウルス感染血清疫学調査 (2017)
  • Author(s): 早坂大輔、内田玲麻 、Mya Myat Ngwe Tun、嶋田聡、余福勲 、田口裕香、佐藤和也、森田公一
  • Organizer: 第52回日本脳炎ウイルス生態学研究会
• [Presentation] 長崎県のイノシシにおけるダニ媒介性ウルス感染血清疫学調査 (2017)
  • Author(s): 早坂大輔
  • Organizer: 第25回ダニと疾患のインターフェースに関するセミナー
• [Presentation] 節足動物媒介性感染症の現状と課題 (2017)
  • Author(s): 高野愛、鍬田龍星、下田宙、前田健
  • Organizer: 第160回日本獣医学会学術集会
• [Presentation] Detection and isolation of tick-borne bacteria in Aponomma lucasi ticks on lizard (Varanus salvator) in Indonesia (2017)
  • Author(s): Supriyono, Takano A, Kuwata R, Shimoda H, Panpiansil J, Hadi UK, Setiyono A, Agungpriyono S, Hondo E, Maeda K
  • Organizer: 第160回日本獣医学会学術集会