• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of CTCs (Circulating Tumor Cells) Detection method by Employing Dielectrophoretic Phenomenon

Research Project

Project/Area Number 17H04704
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Life / Health / Medical informatics
Research InstitutionKure National College of Technology (2018-2020)
Fuzzy Logic Systems Institute (2017)

Principal Investigator

Eguchi Masanori  呉工業高等専門学校, 電気情報工学分野, 准教授 (60613594)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥25,220,000 (Direct Cost: ¥19,400,000、Indirect Cost: ¥5,820,000)
Fiscal Year 2020: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Keywords循環腫瘍細胞 / 誘電泳動 / 絶縁体ベース誘電泳動 / エレクトロローテーション / 計測工学 / 癌 / 画像診断 / インピーダンス計測
Outline of Final Research Achievements

In this study, we developed microwell arrays and micropillar electrode arrays that can generate electro-rotation as basic technologies (devices) for detecting circulating tumor cells. We measured the dielectric properties of a human lung cancer-derived cell line (PC-9) and demonstrated their usefulness. In addition, we developed an insulator-based dielectrophoresis device that can measure the dielectric properties of cells at high speed. This device consists of an aluminum electrode and a creek-gap-shaped insulator on a glass substrate, and is capable of measuring both positive and negative dielectrophoretic forces. In addition, It can measure the dielectric properties by frequency sweeping.

Academic Significance and Societal Importance of the Research Achievements

本研究は,Cellsearchで問題となっている検出感度の向上だけでなく,抗原抗体反応や免疫染色等を行わずラベルフリーかつ全自動で検出可能な装置の実現にむけた基盤技術を確立する.本研究で開発する基板技術により,循環腫瘍細胞を非侵襲に高感度検出,細胞判別ができ,検出した循環腫瘍細胞を用いて抗癌剤や分子標的療法薬等の評価を行うことが可能となる.

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (31 results)

All 2021 2020 2019 2018

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (27 results) (of which Int'l Joint Research: 16 results)

  • [Journal Article] Performance of Ag-Ag2S core-shell nanoparticle-based random network reservoir computing device2021

    • Author(s)
      Hadiyawarman, Yuki Usami, Takumi Kotooka, Saman Azhari, Masanori Eguchi, Hirofumi Tanaka
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 60 Issue: SC Pages: SCCF02-SCCF02

    • DOI

      10.35848/1347-4065/abe206

    • NAID

      120007193028

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Control of the neuromorphic learning behavior based on the aggregation of thiol-protected Ag-Ag2S core?shell nanoparticles2019

    • Author(s)
      Hadiyawarman、Eguchi Masanori、Tanaka Hirofumi
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 59 Issue: 1 Pages: 015001-015001

    • DOI

      10.7567/1347-4065/ab5c77

    • NAID

      120007038823

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Insulator‐based dielectrophoresis combined with the isomotive AC electric field and applied to single cell analysis2019

    • Author(s)
      Tada Shigeru、Eguchi Masanori、Okano Kohei
    • Journal Title

      ELECTROPHORESIS

      Volume: 40 Issue: 10 Pages: 1494-1497

    • DOI

      10.1002/elps.201800380

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of the dielectrophoretic proper- ties of cells using the isomotive AC electric field2018

    • Author(s)
      Shigeru Tada, Yui Omi, Masanori Eguchi
    • Journal Title

      Biomicrofluidics

      Volume: 12 Issue: 4 Pages: 044103-044103

    • DOI

      10.1063/1.5031054

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] 電界結合方式を用いた非接触給電型誘電泳動デバイス2021

    • Author(s)
      堀越悠斗, 藤井蒼太, 氷室貴大, 江口正徳, 山川烈
    • Organizer
      2021年電子情報通信学会総合大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Creek-gap型絶縁体を有する絶縁体ベース誘電泳動デバイスの開発2021

    • Author(s)
      中林龍, 松村浩太朗, 氷室貴大, 江口正徳
    • Organizer
      2021年電子情報通信学会総合大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] DNAが架橋した先端鋭利型電極の電気的特性解析2021

    • Author(s)
      奥清明, 西林寛大, 氷室貴大, 江口正徳
    • Organizer
      2021年電子情報通信学会総合大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Evaluation of Electrode Design of a Microfluidic Impedance Biosensor for DNase Assay2021

    • Author(s)
      Kanta Nishibayashi, Seimei Oku , Takahiro Himuro and Masanori Eguchi
    • Organizer
      International Symposium on Advances in Technology Education 2021
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 誘電泳動現象を用いた生体細胞単離法に関する研究2021

    • Author(s)
      中岡 佑輔, 岡田 麻美, 梶谷 直人, 江口 正徳
    • Organizer
      化学とマイクロ・ナノシステム学会第43回研究会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 生体細胞の誘電特性測定を目指した絶縁体ベース誘電泳動デバイスの設計2021

    • Author(s)
      中林 龍, 江口 正徳
    • Organizer
      化学とマイクロ・ナノシステム学会第43回研究会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 4重極型キャピラリー誘電泳動デバイスによる細胞分離2020

    • Author(s)
      大地 健吾, 多田 茂, 江口 正徳
    • Organizer
      日本機械学会年次大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Improvement on Identification Technique of Primary Tumor Using Ring Resonator with Oscillator Circuit at Centimeter Frequency Bands2020

    • Author(s)
      Shouta Sora, Futoshi Kuroki, Masanori Eguchi, Takeshi Yamakawa, and Fumihiro Tanaka
    • Organizer
      2020 IEEE Radio and Wireless Symposium (RWS)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Tweezers-Types of Electrodes in Video-assisted Thoracic Surgery for Lung Cancer at High Frequency Bands2020

    • Author(s)
      Masaya Sakamoto, Futoshi Kuroki, Masanori Eguchi, Takeshi Yamakawa, Fumihiro Tanaka
    • Organizer
      2020 IEEE Radio and Wireless Symposium (RWS)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Short-term and long-term memory of random aggregation device using Ag-Ag2S nanoparticles2019

    • Author(s)
      Hadiyawarman, Masanori Eguchi and Hirofumi Tanaka
    • Organizer
      第66回応用物理学会春季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] An Approach to Improve Quality-factor of Lossy Ring-resonator Type of Electrode for Circulating Tumor Cell Detection2019

    • Author(s)
      Shouta Sora, Kousei Kumahara, Futoshi Kuroki, Masanori Eguchi, Takeshi Yamakawa, Fumihiro Tanaka
    • Organizer
      2019 49th European Microwave Conference (EuMC)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] An Attempt to Evaluate Potential Hydrogen in Soil Using AM Radio Waves for Agricultural Applications2019

    • Author(s)
      Yuto Uchida, Kousei Kumahara, Tomoki Sakogawa, Futoshi Kuroki, Masanori Eguchi
    • Organizer
      2019 49th European Microwave Conference (EuMC)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Estimation of electric properties of mammalian cells using the creek-gap dielectrophoretic electrode2019

    • Author(s)
      N. Sato, S. Tada, M. Eguchi
    • Organizer
      The 10th Asian-Pacific Conference on Biomechanics
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Consideration on Estimation of Lung Cancer Position Using Tweezers-Types of Electrodes in High Frequency Bands2019

    • Author(s)
      Masaya Sakamoto, Futoshi Kuroki, Masanori Eguchi, Takeshi Yamakawa, and Fumihiro Tanaka
    • Organizer
      2019 Asia-Pacific Microwave Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Improvement on Identification Accuracy of Primary Tumor Using Ring Resonator Type of Electrode at Centimeter Frequencies2019

    • Author(s)
      Shouta Sora, Futoshi Kuroki, Masanori Eguchi, Takeshi Yamakawa, and Fumihiro Tanaka
    • Organizer
      2019 Asia-Pacific Microwave Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Numerical and Experimental Investigations on Ring Resonator Type of Electrode for Circulating Tumor Cell Detection2019

    • Author(s)
      Shouta Sora, Kousei Kumahara, Masanori Eguchi, Futoshi Kuroki, Takeshi Yamakawa, and Fumihiro Tanaka
    • Organizer
      2019 IEEE Radio and Wireless Symposium (RWS)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] リング共振器型電極を用いた循環腫瘍細胞検出の高分解能化に関する検討2019

    • Author(s)
      空 翔太, 熊原 宏征, 江口 正徳, 黒木 太司, 山川 烈, 田中 文啓
    • Organizer
      電子情報通信学会マイクロ波研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 負性抵抗素子を用いた循環腫瘍細胞検出リング共振器型電極のQ値改善に関する検討2019

    • Author(s)
      空 翔太, 熊原 宏征, 江口 正徳, 黒木 太司, 山川 烈, 田中 文啓
    • Organizer
      電子情報通信学会総合大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] A Consideration on Detection of Circulating Tumor Cells Using Ring Resonator Type of Electrode2018

    • Author(s)
      Shouta Sora, Kousei Kumahara, Futoshi Kuroki, Masanori Eguchi, Hiroko Imasato, Takeshi Yamakawa, Kazue Yoneda, Fumihiro Tanaka
    • Organizer
      IEEE AP-S and URSI Int. Symp., 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 対向型循環腫瘍細胞検出マイクロ電極を構成するマイクロストリップ線路の伝送損失と Q値に関する検討2018

    • Author(s)
      空 翔太, 熊原 宏征, 江口 正徳, 黒木 太司, 山川 烈, 田中 文啓
    • Organizer
      電気情報通信学会ソサイエティ大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Experimental Investigation on Potentiality for Circulating Tumor Cell Detection Using Ring-resonator-shaped Electrode2018

    • Author(s)
      Shouta Sora, Kousei Kumahara, Futoshi Kuroki, Masanori Eguchi, Takeshi Yamakawa, and Fumihiro Tanaka
    • Organizer
      6th Smart City Workshop 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Orthogonally-face-to-face Type of Micro-electrode with Ring Resonator for Circulating Tumor Cell Detection2018

    • Author(s)
      Shouta Sora, Kousei Kumahara, Masanori Eguchi, Futoshi Kuroki, Takeshi Yamakawa, and Fumihiro Tanaka
    • Organizer
      2018 Asia-Pacific Microwave Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] リング共振器型電極を用いた循環腫瘍細胞検出の基礎検討2018

    • Author(s)
      空 翔太, 熊原 宏征, 江口 正徳, 黒木 太司, 山川 烈, 田中 文啓
    • Organizer
      電子情報通信学会マイクロ波研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Development of Pillar Electrode Array for Electrorotation Analysis of Single Cells2018

    • Author(s)
      Masanori Eguchi, Keiichi Horio, Futoshi Kuroki, Hiroko Imasato and Takeshi Yamakawa
    • Organizer
      World Automation Congress 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of Dielectrophoretic Properties of Cells by the Use of the Uniform Field Gradient2018

    • Author(s)
      Shigeru Tada, Yui Omi, Masanori Eguchi, Noriko Nakai and Akira Tsukamoto
    • Organizer
      World Automation Congress 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Consideration of Relationship between Shape and Angular Velocity of Particles under Electrorotation2018

    • Author(s)
      Keiichi Horio, Koya Matsuyama, Masanori Eguchi and Takeshi Yamakawa
    • Organizer
      World Automation Congress 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Numerical Investigation of Circulating Tumor Cell Detection Using Resonant Circuits2018

    • Author(s)
      Shouta Sora, Kousei Kumahara, Futoshi Kuroki, Masanori Eguchi, Takeshi Yamakawa, and Fumihiro Tanaka
    • Organizer
      World Automation Congress 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research

URL: 

Published: 2017-04-28   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi