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Deciphering metabolic behaviors of individual microbes in activated sludge by metatranscriptomics

Research Project

Project/Area Number 17H04716
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Modeling and technologies for environmental conservation and remediation
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Sato Yuya  国立研究開発法人産業技術総合研究所, エネルギー・環境領域, 主任研究員 (80711291)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥24,180,000 (Direct Cost: ¥18,600,000、Indirect Cost: ¥5,580,000)
Fiscal Year 2019: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2018: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2017: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywords活性汚泥 / 微生物環相互作用 / 微生物生態系 / メタトランスクリプトーム / 捕食性細菌 / メタゲノム / 代謝 / 微生物間相互作用 / 微生物 / 環境 / 種間相互作用 / メタトランスクリプトーム解析 / 共生
Outline of Final Research Achievements

Activated sludge, which is a complex microbial community composed of thousands kinds of microbes, has been used for wastewater treatment for over 100 years. Yet, because of its complexity, wastewater treatment mechanisms in activated sludge has remained unclear. This study aimed to decipher “who does what” in activated sludge. One of the findings of this study is that small minority nitrifiers can govern the overall performance of the activated sludge bioreactors, via interspecific interactions. Disagreement between population abundance and functional importance in the ecosystem was demonstrated; this uncertainty may contribute to the unpredictable deterioration of activated sludge bioreactors. Another finding is important role of predatory bacteria in controlling biomass of activated sludge. Predatory bacteria can contribute to reduce excessively grown microbes in activated sludge; resulting in reduction of microbial wastes.

Academic Significance and Societal Importance of the Research Achievements

自然環境では微生物は単独で存在することなく多種と共在しているが、そのような複雑な微生物コミュニティの中で「個々の微生物が何をしているのか」を明らかにすることは極めて難しい。本研究では、数千種からなる複雑な微生物コミュニティに遺伝子発現解析を適用し、個々の微生物の挙動を代謝レベルで解明することに成功しており、その技術的な進捗に大きな価値がある。また、研究対象に扱った活性汚泥は世界中で廃水処理に使われており、特定された重要微生物の情報や関連する知見は学術だけでなく産業的にも非常に重要である。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (19 results)

All 2020 2019 2018 2017 Other

All Journal Article (6 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (12 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Remarks (1 results)

  • [Journal Article] Transition of microbial community structures after development of membrane fouling in membrane bioreactors (MBRs)2020

    • Author(s)
      Sato Yuya、Zhao Yan-Jie、Hori Tomoyuki、Aoyagi Tomo、Inaba Tomohiro、Aizawa Hidenobu、Ogata Atsushi、Habe Hiroshi
    • Journal Title

      AMB Express

      Volume: 10 Issue: 1 Pages: 1-10

    • DOI

      10.1186/s13568-020-0959-2

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ごくわずかな硝化細菌が活性汚泥による重油分解の効率を左右する2020

    • Author(s)
      佐藤由也、堀知行、小池英明、羽部浩
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 78 Pages: 116-117

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Transcriptome analysis of activated sludge microbiomes reveals an unexpected role of minority nitrifiers in carbon metabolism2019

    • Author(s)
      Sato Yuya、Hori Tomoyuki、Koike Hideaki、Navarro Ronald R.、Ogata Atsushi、Habe Hiroshi
    • Journal Title

      Communications Biology

      Volume: 2 Issue: 1 Pages: 179-179

    • DOI

      10.1038/s42003-019-0418-2

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Activated sludge microbial communities of a chemical plant wastewater treatment facility with high-strength bromide ions and aromatic substances2019

    • Author(s)
      Zhao Yan-Jie†、Sato Yuya†、Inaba Tomohiro、Aoyagi Tomo、Hori Tomoyuki、Habe Hiroshi
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 65 Issue: 2 Pages: 106-110

    • DOI

      10.2323/jgam.2018.05.002

    • NAID

      130007651705

    • ISSN
      0022-1260, 1349-8037
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 食べる:捕食の関係が作り出す水処理技術の新たな展開2018

    • Author(s)
      佐藤由也、稲葉知大、青柳智、堀知行、羽部浩
    • Journal Title

      環境バイオテクノロジー学会誌

      Volume: 18 Pages: 21-26

    • Related Report
      2018 Annual Research Report
  • [Journal Article] メタトランスクリプトーム解析:RNA-seqで環境を診る2018

    • Author(s)
      佐藤由也、小池英明
    • Journal Title

      生物工学

      Volume: 96 Pages: 403-407

    • Related Report
      2018 Annual Research Report
  • [Presentation] 活性汚泥の網羅的遺伝子発現解析(de novo RNA-seq)で見えた希少種の決定的な役割2018

    • Author(s)
      佐藤由也、堀知行、小池英明、Navaro Ronald R.、尾形敦、羽部浩
    • Organizer
      環境バイオテクノロジー学会2018年度大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 微生物コミュニティにおける捕食性細菌の代謝戦略2018

    • Author(s)
      佐藤由也、稲葉知大、堀知行、羽部浩
    • Organizer
      日本微生物生態学会第32回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] De novo RNA-seq deciphers an unexpected metabolic link between C and N in a complex microbiome2018

    • Author(s)
      佐藤由也、堀知行、小池英明、Navaro Ronald R.、尾形敦、羽部浩
    • Organizer
      ISME17 (17th International Symposium on Microbial Ecology)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 活性汚泥における微生物捕食メカニズム解析と汚泥減容化への応用2018

    • Author(s)
      佐藤由也、稲葉知大、堀知行、成廣隆、羽部浩
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] De novo メタトランスクリプトーム解析とメタボローム解析の併用による捕食性細菌群の代謝戦略の解明2018

    • Author(s)
      佐藤由也、稲葉知大、堀知行、羽部浩
    • Organizer
      佐藤由也、稲葉知大、堀知行、羽部浩
    • Related Report
      2018 Annual Research Report
  • [Presentation] De novo RNA-seq:微生物群集の挙動から環境中の反応をあぶり出す2017

    • Author(s)
      佐藤由也
    • Organizer
      第77回分析化学討論会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] メタトランスクリプトーム解析による活性汚泥中の微生物捕食の特徴づけ2017

    • Author(s)
      佐藤由也、堀知行、稲葉知大、Navarro Ronald R.、尾形敦、羽部浩
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] De novo RNA-seq:活性汚泥微生物群の網羅的遺伝子発現解析で見えた希少種の決定的な役割2017

    • Author(s)
      佐藤由也、堀知行、小池英明、Navarro Ronald R.、尾形敦、羽部浩
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] RNA-seqによる活性汚泥中の微生物捕食の特徴付け2017

    • Author(s)
      佐藤由也、稲葉知大、堀知行、羽部浩
    • Organizer
      第20回日本水環境学会シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 活性汚泥における微生物捕食作用の促進要因2017

    • Author(s)
      佐藤由也、稲葉知大、堀知行、羽部浩
    • Organizer
      日本水環境学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 活性汚泥における微生物捕食作用の機構解明と捕食促進による汚泥減容効果2017

    • Author(s)
      佐藤由也、稲葉知大、堀知行、羽部浩
    • Organizer
      本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 食べる:捕食の関係が作り出す水処理技術の新たな展開2017

    • Author(s)
      佐藤由也、稲葉知大、堀知行、羽部浩
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Remarks] 産業技術総合研究所 環境微生物研究グループ

    • URL

      https://unit.aist.go.jp/emri/114envmicrob/index.html

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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