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Identification of biomolecules controlled by food ingredients and elucidation of their functions

Research Project

Project/Area Number 17H04725
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Eating habits
Research InstitutionOkayama University

Principal Investigator

Nakamura Toshiyuki  岡山大学, 環境生命科学研究科, 助教 (90706988)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥25,740,000 (Direct Cost: ¥19,800,000、Indirect Cost: ¥5,940,000)
Fiscal Year 2020: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2018: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Keywords食品機能 / イソチオシアネート / ポリフェノール / 標的分子
Outline of Final Research Achievements

We investigated the interaction of benzyl isothiocyanate (BITC) with biomolecules. BITC modified ethanolamine was detected from cultured cells after treatment of BITC. It was suggested that phosphatidylethanolamine modified with BITC was degraded by phosphatases. In addition, some proteins were modified with BITC. A correlation was found between the activity and BITC modification in one of the modified proteins, suggesting that BITC directly regulated the protein activity via the modification.

Academic Significance and Societal Importance of the Research Achievements

本研究では食品成分のひとつであるBITCと生体分子の相互作用を示した。食品成分は生体分子と相互作用することにより効果を発揮すると考えられるが、どの生体分子と相互作用して効果を示しているのか未だ十分に明らかではない。そのため、食品機能獲得の個人差や、生活習慣や疾病の有無などによる悪影響への懸念は推測の域を出ない。本研究成果は、このような個人差や悪影響のない個々人に適した食品の選択が可能になり、食品機能の安心安全な獲得に寄与することが期待できる。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (22 results)

All 2021 2020 2019 2018

All Journal Article (10 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 10 results) Presentation (12 results) (of which Int'l Joint Research: 7 results)

  • [Journal Article] Luteolin suppresses 5-hydroxytryptamine elevation in stimulated RBL-2H3 cells and experimental colitis mice2021

    • Author(s)
      Suga N, Murakami A, Arimitsu H, Nakamura T, Nakamura Y, Kato Y.
    • Journal Title

      Journal of Clinical Biochemistry and Nutrition

      Volume: 69 Issue: 1 Pages: 20-27

    • DOI

      10.3164/jcbn.20-192

    • NAID

      130008060391

    • ISSN
      0912-0009, 1880-5086
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A multidrug resistance-associated protein inhibitor is a potential enhancer of the benzyl isothiocyanate-induced apoptosis induction in human colorectal cancer cells.2021

    • Author(s)
      Yang Q, Nakamura T, Seto M, Miyagawa M, Xu W, Zhu B, Munemasa S, Murata Y, Nakamura Y.
    • Journal Title

      Journal of Biochemical and Molecular Toxicology

      Volume: - Issue: 7

    • DOI

      10.1002/jbt.22791

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Stomatal response to isothiocyanates inArabidopsis thaliana2020

    • Author(s)
      Afrin Sonya、Okuma Eiji、Tahjib-Ul-Arif Md、Jahan Md Sarwar、Nakamura Toshiyuki、Nakamura Yoshimasa、Munemasa Shintaro、Murata Yoshiyuki
    • Journal Title

      Journal of Experimental Botany

      Volume: 71 Issue: 22 Pages: 6921-6931

    • DOI

      10.1093/jxb/eraa420

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Characterization of benzyl isothiocyanate extracted from mashed green papaya by distillation2019

    • Author(s)
      Nakamura Toshiyuki、Murata Yoshiyuki、Nakamura Yoshimasa
    • Journal Title

      Food Chemistry

      Volume: 299 Pages: 125118-125118

    • DOI

      10.1016/j.foodchem.2019.125118

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamics of the Cellular Metabolism of Leptosperin Found in Manuka Honey2019

    • Author(s)
      Kato Yoji、Kawai Masaki、Kawai Shota、Okano Yayako、Rokkaku Natsumi、Ishisaka Akari、Murota Kaeko、Nakamura Toshiyuki、Nakamura Yoshimasa、Ikushiro Shinichi
    • Journal Title

      Journal of Agricultural and Food Chemistry

      Volume: 67 Issue: 39 Pages: 10853-10862

    • DOI

      10.1021/acs.jafc.9b03894

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Covalent Modification of Phosphatidylethanolamine by Benzyl Isothiocyanate and the Resultant Generation of Ethanolamine Adduct as Its Metabolite2019

    • Author(s)
      Nakamura Toshiyuki、Hirakawa Miho、Nakamura Yoshimasa、Ishisaka Akari、Kitamoto Noritoshi、Murakami Akira、Kato Yoji
    • Journal Title

      Chemical Research in Toxicology

      Volume: 32 Issue: 4 Pages: 638-644

    • DOI

      10.1021/acs.chemrestox.8b00331

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Methyl-β-cyclodextrin potentiates the BITC-induced anti-cancer effect through modulation of the Akt phosphorylation in human colorectal cancer cells2018

    • Author(s)
      Yang Qifu、Miyagawa Miku、Liu Xiaoyang、Zhu Beiwei、Munemasa Shintaro、Nakamura Toshiyuki、Murata Yoshiyuki、Nakamura Yoshimasa
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 82 Issue: 12 Pages: 2158-2167

    • DOI

      10.1080/09168451.2018.1514249

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Benzyl isothiocyanate ameliorates lipid accumulation in 3T3-L1 preadipocytes during adipocyte differentiation2018

    • Author(s)
      Liang Ying、Sasaki Ikumi、Takeda Yuki、Zhu Beiwei、Munemasa Shintaro、Nakamura Toshiyuki、Murata Yoshiyuki、Nakamura Yoshimasa
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 82 Issue: 12 Pages: 2130-2139

    • DOI

      10.1080/09168451.2018.1514247

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Physiological relevance of covalent protein modification by dietary isothiocyanates2018

    • Author(s)
      Nakamura Toshiyuki、Abe-Kanoh Naomi、Nakamura Yoshimasa
    • Journal Title

      Journal of Clinical Biochemistry and Nutrition

      Volume: 62 Issue: 1 Pages: 11-19

    • DOI

      10.3164/jcbn.17-91

    • NAID

      130006301253

    • ISSN
      0912-0009, 1880-5086
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lymphatic metabolites of quercetin after intestinal administration of quercetin-3-glucoside and its aglycone in rats2018

    • Author(s)
      Nakamura T, Kinjo C, Nakamura Y, Kato Y, Nishikawa M, Hamada M, Nakajima N, Ikushiro S, Murota K.
    • Journal Title

      Arch Biochem Biophys.

      Volume: 645 Pages: 126-136

    • DOI

      10.1016/j.abb.2018.03.024

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] リンゴポリフェノールによるタンパク質ニトロ化の抑制作用2021

    • Author(s)
      江木琴美、Li Kexin、中村俊之、加藤陽二、宗正晋太郎、村田芳行、中村宜督
    • Organizer
      日本農芸化学会中四国支部 第58回講演会(例会)
    • Related Report
      2020 Annual Research Report
  • [Presentation] ベンジルイソチオシアネートは脂肪細胞によるインスリンシグナル伝達を強化する2020

    • Author(s)
      中田小百合、Ying Liang、中村俊之、宗正晋太郎、村田敏行、中村宜督
    • Organizer
      岡山大学次世代研究拠点シンポジウム2020
    • Related Report
      2020 Annual Research Report
  • [Presentation] 食品由来ポリフェノール類がアセトアルデヒドの皮膚表皮細胞への毒性に与える影響2020

    • Author(s)
      明神匠、Xu Wensi、宗正晋太郎、中村俊之、村田芳行、中村宜督
    • Organizer
      岡山大学次世代研究拠点シンポジウム2020
    • Related Report
      2020 Annual Research Report
  • [Presentation] Improvement of the benzyl isothiocyanate stability in aqueous solutions.2019

    • Author(s)
      Toshiyuki Nakamura, Yoshiyuki Murata, Yoshimasa Nakamura
    • Organizer
      The 7th International Conference on Food Factors (ICoFF)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of the target proteins of quercetin glycoside catabolites as cytoprotective agent2019

    • Author(s)
      Kouki Morita, Sayaka Nakashima, Toshiyuki Nakamura, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura
    • Organizer
      The 9th International Conference on Polyphenols and Health (ICPH)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Modulating effects of polyphenols on the acetaldehyde-induced cytotoxicity in human keratinocyte HaCaT cells2019

    • Author(s)
      Takumi Myojin, Wensi Xu, Shintaro Munemasa, Toshiyuki Nakamura, Yoshiyuki Murata, Yoshimasa Nakamura
    • Organizer
      The 9th International Conference on Polyphenols and Health (ICPH)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The distinguishable absorption and metabolism of quercetin coexisted with other food components2019

    • Author(s)
      Rikito Mitsuzane, Toshiyuki Nakamura, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura
    • Organizer
      The 7th International Conference on Food Factors (ICoFF)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characterization of the monoclonal antibody to α-carboxyethyl hydroxychroman2019

    • Author(s)
      Daiki Moriya, Toshiyuki Nakamura, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura
    • Organizer
      The 7th International Conference on Food Factors (ICoFF)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Difference in antioxidant capacity of rice extracts among rice categories and degree of milling and processing2019

    • Author(s)
      Hongyan Wu, Miho Hirooka, Toshiyuki Nakamura, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura
    • Organizer
      The 7th International Conference on Food Factors (ICoFF)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Benzyl isothiocyanate enhances insulin signaling in adipocytes2019

    • Author(s)
      Sayuri Nakata, Ying Liang, Toshiyuki Nakamura, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura
    • Organizer
      The 7th International Conference on Food Factors (ICoFF)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 青パパイヤ蒸留抽出液の生理活性および安定性の評価2018

    • Author(s)
      中村俊之、村田芳行、中村宜督
    • Organizer
      日本フードファクター学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] イソチオシアネート-リジン付加体の薬物代謝酵素発現への影響2018

    • Author(s)
      中村 俊之、加藤 陽二、村田 芳行、中村 宜督
    • Organizer
      農芸化学会
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2022-01-27  

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