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Development of multidimensional molecular diagnostic system based on high-throughput molecular tensile testing

Research Project

Project/Area Number 17H04746
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Medical systems
Research InstitutionUniversity of Yamanashi

Principal Investigator

UKITA Yoshiaki  山梨大学, 大学院総合研究部, 准教授 (40578100)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥26,000,000 (Direct Cost: ¥20,000,000、Indirect Cost: ¥6,000,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2017: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Keywords分子間相互作用 / 生体分子間相互作用 / 分子間相互作用解析技術 / 分子引張試験 / 力学スペクトル / 生体材料
Outline of Final Research Achievements

In this study, we worked on the development of a new molecular analysis technology by mechanically measuring the binding of antigen-antibody reaction. The principle of this technology is based on a molecular tensile test by applying centrifugal force, and it is suitable for simultaneous measurements of multiple single molecular interactions. The main results obtained from this research are (1) development of a comprehensive molecular tensile test system, (2) development of adhesion measurement technology for about 1000 particles, and (3) elucidation of physical interactions between particle substrates (4). Development of physical interaction reduction methods.

Academic Significance and Societal Importance of the Research Achievements

免疫反応を用いるセンサーは免疫センサーと呼ばれ、医療、環境、食品等幅広い分野で活用されている。一方、免疫反応が高い特異性を有するとは言え、超高感度測定等精密な測定においては、狭雑分子の誤認識が問題になるケースがある。これは、従来型の免疫測定系がセンサーに結合した物質の量を指標としていることによると考えた。そこで、本研究では分子の結合力測定原理を新たに開発することで、結合の質の評価に基づく新発想の免疫センサーを実現することを目的とした。これにより、信頼性の高い前立腺癌マーカーの解析技術の実現に貢献できると考える。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (8 results)

All 2021 2020 2019 2018

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Dynamic Measurement Method for Bio-molecular Interactions by Using Centrifugal Force2019

    • Author(s)
      Mao Otake, Yoshiaki Ukita
    • Journal Title

      Analytical Sciences

      Volume: 35 Issue: 10 Pages: 1123-1127

    • DOI

      10.2116/analsci.19P137

    • NAID

      130007726240

    • ISSN
      0910-6340, 1348-2246
    • Year and Date
      2019-10-10
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Force Analysis Method of Single Molecule Interaction Using Centrifugal Force2019

    • Author(s)
      Mao Otake, Yoshiaki Ukita
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 58 Issue: SI Pages: SIIC03-SIIC03

    • DOI

      10.7567/1347-4065/ab1b5e

    • NAID

      210000156642

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 固相表面へのナノ粒子修飾による平面と粒 子間の界面相互作用の制御2021

    • Author(s)
      大竹 真央,久米 真司,浮田 芳昭
    • Organizer
      化学とマイクロ・ナノシステム学会 第43回研究会(CHEMINAS 43)
    • Related Report
      2020 Annual Research Report
  • [Presentation] 画像投影法による付着微粒子のスクリーニング2020

    • Author(s)
      大竹真央、浮田芳昭
    • Organizer
      第 67 回応用物理学会春季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Dynamic analysis method of single molecule interaction using centrifugal force2019

    • Author(s)
      Mao Otake, Yoshiaki Ukita
    • Organizer
      14th International Conference on Atomically Controlled Surfaces, Interfaces and Nanostructures (ACSIN-14)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 分子間相互作用並列測定のための抗体修飾基板における架橋剤の検討2019

    • Author(s)
      大竹 真央,浮田 芳昭
    • Organizer
      第66回応用物理学会春季学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] マイクロ流体工学を基盤とする免疫測定技術の開発2019

    • Author(s)
      浮田芳昭
    • Organizer
      平成30年度 日本分析化学会関東支部山梨地区交流会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] ハイスループットな単一分子の力学解析法の提案2018

    • Author(s)
      大竹真央、浮田芳昭
    • Organizer
      第65回応用物理学会春期学術講演会
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2022-01-27  

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