| Budget Amount *help |
¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2017: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
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| Outline of Final Research Achievements |
Dopamine plays pivotal roles in diverse cognitive functions ranging from reward and motivation to feeding and sexual behaviors. Differential modulatory effects of dopamine neurotransmission may be defined by distinct expression profiles of functionally different receptor subtypes. We thus conducted systematic expression profiling of four dopamine receptors in the memory center of fruit flies, the mushroom body. By utilizing CRISPR/CAS9 genome editing techniques, we characterized the endogenous receptor gene and protein expressions. We found cellular and subcellular expressions distinct among receptor subtypes, which may characterize their functions as well as the functionality of the mushroom body's subcompartments. Our findings may provide neuroanatomical logic for dopamine neurotransmission causing opposing effects across different subdomains of a brain structure, which supports adaptive behaviors in an animal.
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