| Budget Amount *help |
¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2017: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
This study is to develop a scanning probe microscopy technique that can visualize the nanoscale physical dynamics occurring in the living cell surface. In order to visualize and measure the cell surface without damage, we significantly improved in actual performances of scanning ion conductance microscopy. By developing a high-speed scanner and its vibration control technique, improving the signal-to-noise ratio of the high-resolution nanopipette probe, and improving the stability of the ion conduction measurement, we were able to visualize the dynamics of the nanostructures on living cell surfaces within a few seconds per frame. The spatiotemporal resolution of our developed system demonstrated the highest performance than other existing similar scanning probe techniques. Furthermore, we have succeeded in developing a technique to simultaneously measure the topography of cell surface and physical properties, such as the local mechanical properties and local surface charge density.
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