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Visualization of dynamics of nanoscale physical properties of living cell surface probed by high-speed ion conductance microscopy

Research Project

Project/Area Number 17H04818
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field General applied physics
Research InstitutionKanazawa University

Principal Investigator

Watanabe Shinji  金沢大学, ナノ生命科学研究所, 准教授 (70455864)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2017: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywordsプローブ顕微鏡 / ナノピペット / ナノポア / 生細胞イメージング / イオン伝導顕微鏡 / 表面電荷 / 生細胞 / 生体膜 / ピペット / 電気伝導計測 / イオン電流 / 細胞 / ラフト / 走査プローブ顕微鏡 / 物理計測・制御
Outline of Final Research Achievements

This study is to develop a scanning probe microscopy technique that can visualize the nanoscale physical dynamics occurring in the living cell surface. In order to visualize and measure the cell surface without damage, we significantly improved in actual performances of scanning ion conductance microscopy. By developing a high-speed scanner and its vibration control technique, improving the signal-to-noise ratio of the high-resolution nanopipette probe, and improving the stability of the ion conduction measurement, we were able to visualize the dynamics of the nanostructures on living cell surfaces within a few seconds per frame. The spatiotemporal resolution of our developed system demonstrated the highest performance than other existing similar scanning probe techniques. Furthermore, we have succeeded in developing a technique to simultaneously measure the topography of cell surface and physical properties, such as the local mechanical properties and local surface charge density.

Academic Significance and Societal Importance of the Research Achievements

生物の境界の世界である、細胞表層で生じている様々な物理動態を計測する技術は、生命現象を理解ための重要な計測技術となるに違いない。本研究は、細胞表層のナノ形状動態の可視化技術や、ナノ物性の動態を計測する技術の開発を推進し、従来計測技術の性能を大きく向上した。これによって、従来は捉えることが不可能だった細胞表層の生命現象を計測できる可能性を大きく広げた。

Report

(4 results)
  • 2020 Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (50 results)

All 2020 2019 2018 2017

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (44 results) (of which Int'l Joint Research: 16 results,  Invited: 7 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Thermally driven approach to fill sub-10-nm pipettes with batch production2019

    • Author(s)
      L. Sun, K. Shigyou, T. Ando, S. Watanabe
    • Journal Title

      Anal. Chem.

      Volume: 91 Issue: 21 Pages: 14080-14084

    • DOI

      10.1021/acs.analchem.9b03848

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of high-speed ion conductance microscopy2019

    • Author(s)
      S. Watanabe, S. Kitazawa, L. Sun, N. Kodera, T. Ando
    • Journal Title

      Rev. Sci. Instrum.

      Volume: 90 Issue: 12 Pages: 123704-123704

    • DOI

      10.1063/1.5118360

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-speed XYZ nanopositioner for scanning ion conductance microscopy2017

    • Author(s)
      S. Watanabe and T. Ando
    • Journal Title

      Appl. Phys. Lett.

      Volume: 111 Issue: 11 Pages: 113106-113106

    • DOI

      10.1063/1.4993296

    • NAID

      120006370925

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] Sub-10nmナノピペットのTEM観察とその電気抵抗の理論解析2020

    • Author(s)
      執行 航希, Sun Linhao, 滝ヶ浦 尚平, 矢島 陸, 田嶋 将, 田岡 東, 宮澤 佳甫, 福間 剛士, 渡邉 信嗣, 安藤 敏夫
    • Organizer
      第67回応用物理学会春季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ガラスナノピペットを用いた探針増強ラマン散乱2020

    • Author(s)
      矢島 陸, 執行 航希, 安藤 敏夫, 渡辺 信嗣
    • Organizer
      第67回応用物理学会春季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 高速イオン伝導顕微鏡による細胞表層物性のナノスケールマッピング2020

    • Author(s)
      Linhao Sun, 開発 秀星, 北澤 怜子, 芳坂 綾子, 渡辺信嗣
    • Organizer
      第67回応用物理学会春季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 高速SICMによる生細胞イメージング2019

    • Author(s)
      渡辺信嗣
    • Organizer
      学振167委員会 第91回研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 高速イオン伝導顕微鏡による生細胞のナノ計測2019

    • Author(s)
      渡辺信嗣
    • Organizer
      日本顕微鏡学会第75回学術講演会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] High-speed ion conductance microscopy for probing nanoscale surface properties of live cells2019

    • Author(s)
      Shinji Watanabe
    • Organizer
      M&BE10
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 高速イオン伝導顕微鏡で計測する生細胞界面のナノスケールの物理挙動2019

    • Author(s)
      渡辺信嗣
    • Organizer
      第9回分子モーター討論会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ガラスナノピペットを用いた非標識単分子検出センサーの開発2019

    • Author(s)
      矢島 陸, 渡辺信嗣
    • Organizer
      第9回分子モーター討論会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 高速走査型イオン伝導顕微鏡による表面電荷イメージング2019

    • Author(s)
      開発 秀星, 執行 航希, 安藤 敏夫, 渡辺 信嗣
    • Organizer
      第80回応用物理学会秋季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 走査型イオン伝導顕微鏡による生細胞表面の機械的性質のマッピング2019

    • Author(s)
      北澤 怜子, Sun Linhao, 芳坂 綾子, 中山 隆宏, 紺野 宏記, 柴田 幹大, 渡辺 信嗣
    • Organizer
      第80回応用物理学会秋季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Mapping of surface charge by high speed ion conductance microscopy2019

    • Author(s)
      開発 秀星, 執行 航希, 安藤 敏夫, 渡辺 信嗣
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Mapping of mechanical property on live cell surface by scanning ion conductance microscope2019

    • Author(s)
      北澤 怜子, Sun Linhao, 芳坂 綾子, 中山 隆宏, 紺野 宏記, 柴田 幹大, 渡辺 信嗣
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 高速イオン伝導顕微鏡による生細胞表層のナノスケール物性マッピング2019

    • Author(s)
      渡辺信嗣
    • Organizer
      2019年日本表面真空学会学術講演会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Morphological Dynamics and Mechanics of Drug-Stimulated Living Cell Visualized by High Speed Scanning Ion Conductance Microscope (HS-SICM)2019

    • Author(s)
      L. Sun, S. Kitazawa, S. Watanabe
    • Organizer
      33rd International Microprocesses and Nanotechnology Conference (MNC2020)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Nanoscale visualization of live cell surface by high-speed ion conductance microscopy2019

    • Author(s)
      Shinji Watanabe
    • Organizer
      Joint USBI-NanoLSI workshop
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Visualization of nanomechanical responses of living cell surface captured by high-speed ion conductance microscope2019

    • Author(s)
      S. Kaihatsu, K. Shigyou, T. Ando, S. Watanabe
    • Organizer
      ICSPM27
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Visualization of surface charge difference of lipid bilayers on mica substrate by scanning ion conductance microscopy2019

    • Author(s)
      S. Kitazawa, L. Sun, A. Housaka, T. Watanabe-Nakayama, H. Konno, S. Watanabe
    • Organizer
      ICSPM27
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Modulated 1/f^a Noise Behavior by Bias Voltage and Concentration Gradient in Conically Shaped Quartz Nanopipettes2019

    • Author(s)
      Linhao Sun, Kazuki shigyou, Toshio Ando, Shinji Watanabe
    • Organizer
      2019年第66回応用物理学会春季学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 高速イオン伝導顕微鏡による脂質膜のナノ計測2019

    • Author(s)
      渡辺 信嗣, 開発 秀星, 北澤 怜子, 田嶋 将, Sun Linhao, 芳坂 綾子, 執行 航希, 安藤 敏夫
    • Organizer
      2019年第66回応用物理学会春季学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 高速イオン伝導顕微鏡の開発2018

    • Author(s)
      渡辺信嗣
    • Organizer
      M&BE分科会6月研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] バイオ物質をリアルタイムで観察する!2018

    • Author(s)
      渡辺信嗣
    • Organizer
      新潟大学振興会技術講演会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Development of nanoscale mapping of surface charge density of lipid membranes by high-speed ion conductance microscopy2018

    • Author(s)
      開発 秀星、執行 航希、安藤 敏夫、渡辺 信嗣
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Observation of Morphological Changes in Nanostructures on Live Cell Surfaces by High Speed Ion Conductance Microscopy2018

    • Author(s)
      北澤 怜子、芳坂 綾子、中山 隆宏、紺野 宏記、渡辺 信嗣
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 高速イオン伝導顕微鏡による細胞表面のナノ構造体の形態変化の観察2018

    • Author(s)
      北澤 怜子、芳坂 綾子、中山 隆宏、紺野 宏記、渡辺 信嗣
    • Organizer
      第79回応用物理学会秋季学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 走査型イオン伝導顕微鏡による脂質膜の表面電荷密度の高速検出2018

    • Author(s)
      開発 秀星、執行 航希、安藤 敏夫、渡辺 信嗣
    • Organizer
      第79回応用物理学会秋季学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Nanoscale Mapping of Surface Charge Density of Lipid Membranes by Scanning Ion Conductance Microscopy2018

    • Author(s)
      S. Kaihatsu、K. Shigyo、T. Ando、S. Watanabe
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 走査型イオン伝導顕微鏡による脂質膜の表面電荷密度の高速検出2018

    • Author(s)
      開発 秀星、執行 航希、安藤 敏夫、渡辺 信嗣
    • Organizer
      第ACSIN-14 & ICSPM26
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Morphological Changes in Nanostructures on Living Cell Surface Captured by High-Speed Ion Conductance Microscopy2018

    • Author(s)
      S. Kitazawa, A. Housaka, T. W. Nakayama, H. Konno, S. Watanabe
    • Organizer
      第ACSIN-14 & ICSPM26
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Visualization and Characterization of Biological Mebrane Structures using High-Speed Ion Conductance Microscopy2018

    • Author(s)
      Shinji Watanabe
    • Organizer
      The 2nd NanoLSI International Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Exploring 1/f noise behavior of ion current in a single channel of conically shaped pipette filled with electrolyte solution2018

    • Author(s)
      Linhao Sun, Kazuki Shigyo, Shohei Takigaura, and Shinji Watanabe
    • Organizer
      第65回応用物理学会春季学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] カーボンナノチューブを利用したSICMプローブの作成2018

    • Author(s)
      執行 航希、Yao Yun-Chiao、Noy Alexsandr
    • Organizer
      第65回応用物理学会春季学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] イオン伝導顕微鏡による生細胞表面の観察2018

    • Author(s)
      北澤怜子、渡辺 信嗣
    • Organizer
      日本生物物理学会中部支部講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 走査型プローブ顕微鏡の時間分解能向上に向けた水平走査制御手法の開発2018

    • Author(s)
      開発秀星、渡辺 信嗣
    • Organizer
      日本生物物理学会中部支部講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] High-speed Ion Conductance Microscope2018

    • Author(s)
      Shinji Watanabe
    • Organizer
      The 1st NanoLSI International Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ガラスピペットを用いたナノ解像顕微鏡の開発2017

    • Author(s)
      渡辺 信嗣
    • Organizer
      第8回ライフサイエンス研究交流セミナー
    • Related Report
      2017 Annual Research Report
  • [Presentation] Improvements in Fundamental Performance of Scanning Ion Conductance Microscope for Visualizing Morphological Changes in Biological Samples with Nanoscale2017

    • Author(s)
      Shinji Watanabe
    • Organizer
      25th International Colloquium on Scanning Probe Microscopy
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 高速イオン伝導顕微鏡による液中ナノイメージング技術とその応用2017

    • Author(s)
      渡辺 信嗣
    • Organizer
      日本表面科学会中部支部研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Development of High-Speed Ion Conductance Microscopy for Visualizing Morphological Changes in Biological Samples with Nanoscale Resolution2017

    • Author(s)
      Shinji Watanabe
    • Organizer
      SFS2017 International Symposium on Fluctuation and Structure out of Equilibrium 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] High-speed ion conductance microscopy for visualizing morphological changes in biological samples with nanoscale resolution2017

    • Author(s)
      Shinji Watanabe
    • Organizer
      International Symposium on Atomic Force Microscopy at Solid-Liquid Interfaces
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Controlling Inner Diameter of Nanopipette Tip for High-Resolution Ion Conductance Microscope2017

    • Author(s)
      Shohei Takigaura, Azuma Taoka, Yousuke Kikuchi, Keisuke Miyazawa, Takeshi Fukuma, and Shinji Watanabe
    • Organizer
      International Symposium on Atomic Force Microscopy at Solid-Liquid Interfaces
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A novel approach for making electrical contact of pipettes for high spatial resolution scanning ion conductance microscopy2017

    • Author(s)
      Linhao Sun, and Shinji Watanabe
    • Organizer
      International Symposium on Atomic Force Microscopy at Solid-Liquid Interfaces
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 高速イオン伝導顕微鏡の開発とその応用展開2017

    • Author(s)
      渡辺 信嗣
    • Organizer
      第13回固体イオニクスセミナー
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 高解像イオン伝導顕微鏡のためのナノピペットの先端形状制御2017

    • Author(s)
      滝ヶ浦尚平、田岡東、菊池洋輔、宮澤佳甫、福間剛士、Linhao Sun、渡辺信嗣
    • Organizer
      2017年第64回応用物理学会秋季学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Nanoscale imaging of biological samples using high-speed ion conductance microscopy2017

    • Author(s)
      Shinji Watanabe
    • Organizer
      ゆらぎと構造の協奏 第4回領域研究会
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] SICM用プローブの製造方法及びSICM用プローブ2018

    • Inventor(s)
      滝ヶ浦尚平,渡辺信嗣
    • Industrial Property Rights Holder
      滝ヶ浦尚平,渡辺信嗣
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-033897
    • Filing Date
      2018
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 表面計測方法、イオン伝導顕微鏡およびプローブ2017

    • Inventor(s)
      渡辺信嗣,安藤敏夫
    • Industrial Property Rights Holder
      渡辺信嗣,安藤敏夫
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-172666
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 液体充填方法、SICM用プローブの製造方法、SICM用プローブ及び SICM2017

    • Inventor(s)
      Sun Linhao,執行航希,渡辺信嗣,安藤敏夫
    • Industrial Property Rights Holder
      Sun Linhao,執行航希,渡辺信嗣,安藤敏夫
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-245642
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2025-01-30  

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