| Budget Amount *help |
¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000)
Fiscal Year 2020: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2017: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
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| Outline of Final Research Achievements |
Some copper oxidases bear the cofactors which is generated by post-translationally chemical modification of the corresponding amino acid side chains near the copper active center. If such unusual cofactors are able to be formed on the small peptide and proteins, they will be promising tools for the modification of protein and the direct observation of the labeled-proteins in the cells. In this study, we have prepared rationally designed copper-binding peptides and proteins, which can induce the organic cofactors by posttranslationally chemical modifications. The structures and spectroscopic properties of the generated unusual amino acids were examined by several analytical methods including MS analysis and UV-vis spectroscopy.
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