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Evaluation of mechanical interaction between cell spheroid and culture environment based on mechano-index activated sorting

Research Project

Project/Area Number 17H04913
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Intelligent mechanics/Mechanical systems
Research InstitutionNagoya University

Principal Investigator

Sakuma Shinya  名古屋大学, 工学研究科, 助教 (40724464)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2017: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Keywords機械的特性計測 / スフェロイド / ソーティング / MEMS / microTAS / 機械特性計測
Outline of Final Research Achievements

In this study, we demonstrated a mechano-index activated sorting of cell spheroids based on a robot-integrated microfluidic chip. The chip contains a pair of force sensor probes, where force sensing is based on measuring the deformation of beams of the probes. By utilizing the constructed system, we obtained Young’s modulus of spheroids from the data of deformation and force which was measured by the images taken though a CCD camera. We performed the experiment of continuous mechanical indexing of cell spheroid using 26 MSC spheroids, and the results showed that the measurement throughput of 3.14 spheroids per minutes. Finally, we demonstrated the on-chip sorting based on the mechanical index of Young’s modulus using a mixed solution of MSC spheroids and microbeads, and succeeded in the continuous machine index activated on-chip sorting of MSC: 5/5 and beads: 5/5.

Academic Significance and Societal Importance of the Research Achievements

オンチップソーティング技術は,細胞集団の中から特定の指標に基づきある細胞集団を分取する技術であり,単一の細胞・スフェロイド・オルガノイド解析において重要な技術として広く用いられている.現在は,蛍光活性型細胞ソーティング(FACS)に代表されるように,分取指標として蛍光指標が主流であるが,さらなる詳細な単一細胞の解析,および,従来では見つけることができなかったユニークな細胞の発見・分取のためには,新たな計測モダリティが必要であると考えられる.本研究で構築した機械指標活性型ソーティングは,従来のソーティングモダリティに機械指標という新たなモダリティを付与すると考えられる.

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (20 results)

All 2020 2019 2018 2017

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (17 results) (of which Int'l Joint Research: 7 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] High-Speed On-Chip Mixing by Microvortex Generated by Controlling Local Jet Flow Using Dual Membrane Pumps2019

    • Author(s)
      Yusuke Kasai, Shinya Sakuma, Fumihito Arai
    • Journal Title

      IEEE Robotics and Automation Letters

      Volume: 4(3) Issue: 3 Pages: 2839-2846

    • DOI

      10.1109/lra.2019.2921696

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Continuous Mechanical Indexing of Single-Cell Spheroids Using a Robot-Integrated Microfluidic Chip2019

    • Author(s)
      Shinya Sakuma, Ko Nakahara, Fumihito Arai
    • Journal Title

      IEEE Robotics and Automation Letters

      Volume: 4(3) Issue: 3 Pages: 2973-2980

    • DOI

      10.1109/lra.2019.2923976

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] High-speed and high-resolution on-chip pumping utilizing asymmetric flow resistors2020

    • Author(s)
      Makoto Saito, Yusuke Kasai, Hiroki Kumon, Shinya Sakuma, Fumihito Arai
    • Organizer
      The 33rd International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2020)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Spatiotemporally generated microfluids with the aid of high-speed flow control2019

    • Author(s)
      Yusuke Kasai, Makoto Saito, Shinya Sakuma, Fumihito Arai,
    • Organizer
      The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Spatiotemporally generated microfluids: traveling vortex for on-chip sorting of large particles2019

    • Author(s)
      Yusuke Kasai, Shinya Sakuma, Fumihito Arai
    • Organizer
      The 30th 2019 International Symposium on Micro-NanoMechatronics and Human Science (MHS2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Spatiotemporally generated microfluids: asymmetric flow resistor for on-chip pumping2019

    • Author(s)
      Makoto Saito, Shinya Sakuma, Yusuke Kasai, Fumihito Arai
    • Organizer
      The 30th 2019 International Symposium on Micro-NanoMechatronics and Human Science (MHS2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 超高速流体が拓く非構造化マイクロフルイズ2019

    • Author(s)
      佐久間臣耶,笠井宥佑,齋藤真,新井史人
    • Organizer
      化学とマイクロ・ナノシステム学会第39回研究会(CHEMINAS 39)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 超高速流体制御を用いた層流領域での非対称流生成によるオンチップポンピング2019

    • Author(s)
      齋藤真,佐久間臣耶,笠井宥佑,新井史人
    • Organizer
      第37回日本ロボット学会学術講演会(RSJ2019)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 輸送渦の時空間的生成によるオンチップマルチソーティング2019

    • Author(s)
      佐久間臣耶,笠井宥佑,新井史人
    • Organizer
      化学とマイクロ・ナノシステム学会第40回研究会(CHEMINAS 40)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 定在渦を用いたマイクロ流路の時空間的流体抵抗制御2019

    • Author(s)
      齋藤真,佐久間臣耶,笠井宥佑,新井史人
    • Organizer
      化学とマイクロ・ナノシステム学会第40回研究会(CHEMINAS 40)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 輸送渦の時空間的生成を用いたオンチップソーティング2019

    • Author(s)
      笠井宥佑,佐久間臣耶,新井史人
    • Organizer
      第20回計測自動制御学会システムインテグレーション部門講演会(SI2019)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 非対称マイクロ流体抵抗を用いたオンチップ流体制御2019

    • Author(s)
      齋藤真,佐久間臣耶,笠井宥佑,新井史人
    • Organizer
      第20回計測自動制御学会システムインテグレーション部門講演会(SI2019)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Toward high-throughput sorting of single spheroids based on mechano-index on a microfluidic chip2018

    • Author(s)
      Kou Nakahara, Shinya Sakuma, Fumihito Arai
    • Organizer
      The 29th Int. Symp. on Micro-Nano Mechatronics and Human Science (MHS2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 機械的特徴量に基づいたオンチップ細胞ソーティング2018

    • Author(s)
      中原康, 佐久間臣耶, 新井史人
    • Organizer
      日本機械学会・ロボティクスメカトロニクス講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 機械的指標に基づくセルソーティングシステム の提案2018

    • Author(s)
      中原康, 佐久間臣耶, 新井史人
    • Organizer
      日本ロボット学会学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] On-chip cell separation based on mechanical characteristics2017

    • Author(s)
      Kou Nakahara, Shinya Sakuma, Fumihito Arai
    • Organizer
      The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Automated on-chip sorting system for separation of spheroid based on the mechanical2017

    • Author(s)
      Kou Nakahara, Shinya Sakuma, Fumihito Arai
    • Organizer
      2017 international symposium on Micro-NanoMechatronics and Human Science (MHS2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] スフェロイドの機械特性に基づく分離を目指したオンチップソーティングシステム2017

    • Author(s)
      中原康, 佐久間臣耶, 新井史人
    • Organizer
      化学とマイクロ・ナノシステム学会第35回研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 細胞の機械特性に基づくオンチップ連続ソーティング,ポスター発表2017

    • Author(s)
      中原康, 佐久間臣耶, 新井史人
    • Organizer
      化学とマイクロ・ナノシステム学会第36回研究会
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] マイクロポンプおよび流量の制御方法2019

    • Inventor(s)
      佐久間臣耶,齋藤真,笠井宥佑,新井史人
    • Industrial Property Rights Holder
      佐久間臣耶,齋藤真,笠井宥佑,新井史人
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-159784
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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