| Budget Amount *help |
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2017: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
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| Outline of Final Research Achievements |
In this study, we demonstrated a mechano-index activated sorting of cell spheroids based on a robot-integrated microfluidic chip. The chip contains a pair of force sensor probes, where force sensing is based on measuring the deformation of beams of the probes. By utilizing the constructed system, we obtained Young’s modulus of spheroids from the data of deformation and force which was measured by the images taken though a CCD camera. We performed the experiment of continuous mechanical indexing of cell spheroid using 26 MSC spheroids, and the results showed that the measurement throughput of 3.14 spheroids per minutes. Finally, we demonstrated the on-chip sorting based on the mechanical index of Young’s modulus using a mixed solution of MSC spheroids and microbeads, and succeeded in the continuous machine index activated on-chip sorting of MSC: 5/5 and beads: 5/5.
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