Exploiting sensitive aptasensor technologies for rapid on-site detection of infectious norovirus in the environment
| Project/Area Number |
17H04939
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
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| Keywords | ノロウイルス / 感染性 / アプタセンサー / 下水 / 電気化学 / アプタマー / バイオセンサー / 水環境 / 環境水 |
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| Outline of Final Research Achievements |
In this study, an electrochemical aptasensor for on-site detection of infectious norovirus in the environment was developed by optimizing the electrochemical measurement condition and identifying the measurement condition that enables sensitive norovirus detection. A standard curve with a good linear relationship between the electrochemical signal and murine norovirus concentration was obtained under the optimized measurement condition. Applicability of the electrochemical aptasensor technology to the detection of virus in treated wastewater was evaluated using murine norovirus as a model virus, which demonstrated a decrease of peak current depending on virus concentration. The results of this study indicated that our electrochemical aptasensor technology could detect norovius of as low as 1 virus/L in treated wastewater in combination with a virus concentration method.
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| Academic Significance and Societal Importance of the Research Achievements |
ノロウイルスの感染リスクを抑制するためには、その存在を現場で迅速・高感度に検出し、許容レベル以上のノロウイルス汚染が認められた場合には即座に対策を講じる必要がある。本研究で開発した電気化学アプタセンサー技術は、アプタマー（人工機能性核酸）を検出対象に合わせて変更するだけで任意の病原ウイルスに適用可能であるため、今後、新型コロナウイルスを含むあらゆる病原ウイルスの検出技術に応用できる可能性がある。
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Report
(5 results)
Research Products
(14 results)
