| Budget Amount *help |
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
|
|---|
| Outline of Final Research Achievements |
In this study, an electrochemical aptasensor for on-site detection of infectious norovirus in the environment was developed by optimizing the electrochemical measurement condition and identifying the measurement condition that enables sensitive norovirus detection. A standard curve with a good linear relationship between the electrochemical signal and murine norovirus concentration was obtained under the optimized measurement condition. Applicability of the electrochemical aptasensor technology to the detection of virus in treated wastewater was evaluated using murine norovirus as a model virus, which demonstrated a decrease of peak current depending on virus concentration. The results of this study indicated that our electrochemical aptasensor technology could detect norovius of as low as 1 virus/L in treated wastewater in combination with a virus concentration method.
|
