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Development of the anterograde neural tracing system for understanding the neural circuit for memory

Research Project

Project/Area Number 17H04984
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Neurophysiology / General neuroscience
Research InstitutionKyoto University

Principal Investigator

Hirano Yukinori  京都大学, 白眉センター, 特定准教授 (40580121)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
Fiscal Year 2019: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2018: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2017: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Keywords順行性神経標識 / 神経回路 / ショウジョウバエ / 記憶 / 順行性標識 / 神経ネットワーク
Outline of Final Research Achievements

We aimed to establish a novel technique by which the synaptic connection in the brain is visualized anterogradely. We adopted the artificial protein using Notch transmembrane protein. This artificial protein was optimized in vitro, and tested in Drosophila, which neural circuit can be labeled genetically. We successfully obtained the candidate protein which can be used for our specific aim, and will employ this to understand the complicated neural circuit for memory.

Academic Significance and Societal Importance of the Research Achievements

これまでの様々な技術の発展から、遺伝学的、および生化学的に複雑な脳神経回路が解き明かされてきた。一方で、記憶に代表されるような、限られた記憶神経が司る神経ネットワークを理解するためには、シナプス間結合のある神経のみを標識し、それらを操作する方法論の樹立が必要不可欠であるが、そのような試みはされてこなかった。本研究で確立した順行性標識法が簡易な手法として活用できれば、ウィルスを用いずに遺伝子改変動物で可能となり、脳回路研究の強力な推進力となるだろう。この手法は遺伝子改変が可能なあらゆる動物に応用可能であり、ショウジョウバエ研究にとどまらない、汎用性の高い手法であると考えている。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (6 results)

All 2019

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] The neural circuit linking mushroom body parallel circuits induces memory consolidation in Drosophila Sci U S A , 116 (32), 16080-16085 2019 Aug 62019

    • Author(s)
      Hiroko Awata, Mai Takakura, Yoko Kimura, Ikuko Iwata, Tomoko Masuda, Yukinori Hirano
    • Journal Title

      PNAS

      Volume: 116 Issue: 32 Pages: 16080-16085

    • DOI

      10.1073/pnas.1901292116

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] ショウジョウバエHDACによる非ヒストンタンパクの脱アセチル化を介した遺伝子発現誘導の時間制御2019

    • Author(s)
      Mai Takakura, Yukinori Hirano
    • Organizer
      日本神経科学学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] The Trisynaptic Disinhibitory Network linking Mushroom Body Parallel Circuits Induces Memory Consolidation in Drosophila.2019

    • Author(s)
      Yukinori Hirano
    • Organizer
      日本分子生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Decoding spaced training is mediated by a GABAergic and a trigger neuron in Drosophila.2019

    • Author(s)
      Yukinori Hirano
    • Organizer
      日本神経科学学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] The Trisynaptic Disinhibitory Network linking Mushroom Body Parallel Circuits Induces Memory Consolidation in Drosophila.2019

    • Author(s)
      Yukinori Hirano
    • Organizer
      Latest Advances in Development & Function of Neuronal Circuits
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ショウジョウバエ記憶中枢並列回路をつなぐ脱抑制回路が、遺伝子発現誘導を介した記憶 固定化を誘導する2019

    • Author(s)
      平野恭敬
    • Organizer
      日本神経化学会大会
    • Related Report
      2018 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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